Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.26.21 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ebc29d2a49774f1d91212fc7809d1301",
"name": "M1_20170605_1814_dsRNA_L4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:26:43"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8221 (max 8222) in '8221'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6645
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84991e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.930916
Ran for 1050 frames Cell Events: 59 mean_circ: 0.92765
Ran for 1550 frames Cell Events: 92 mean_circ: 0.931125
Ran for 2050 frames Cell Events: 133 mean_circ: 0.931125
Ran for 2550 frames Cell Events: 153 mean_circ: 0.932464
Ran for 3050 frames Cell Events: 189 mean_circ: 0.932057
Ran for 3550 frames Cell Events: 214 mean_circ: 0.93296
Ran for 4550 frames Cell Events: 265 mean_circ: 0.93119
Ran for 5050 frames Cell Events: 287 mean_circ: 0.931516
Ran for 5550 frames Cell Events: 312 mean_circ: 0.932233
Ran for 6050 frames Cell Events: 338 mean_circ: 0.932733
Ran for 6550 frames Cell Events: 373 mean_circ: 0.932921
Ran for 8050 frames Cell Events: 438 mean_circ: 0.934176
Ran for 8550 frames Cell Events: 458 mean_circ: 0.93397
Ran for 9050 frames Cell Events: 484 mean_circ: 0.934197
Ran for 11050 frames Cell Events: 580 mean_circ: 0.932823
Ran for 11550 frames Cell Events: 604 mean_circ: 0.932812
Ran for 12550 frames Cell Events: 656 mean_circ: 0.932066
Ran for 13050 frames Cell Events: 684 mean_circ: 0.932004
Ran for 13550 frames Cell Events: 721 mean_circ: 0.9325
Ran for 14550 frames Cell Events: 786 mean_circ: 0.932841
Ran for 15050 frames Cell Events: 833 mean_circ: 0.932774
Ran for 17550 frames Cell Events: 989 mean_circ: 0.93409
Ran for 18050 frames Cell Events: 1014 mean_circ: 0.934264
Ran for 18550 frames Cell Events: 1033 mean_circ: 0.934438
Ran for 19050 frames Cell Events: 1056 mean_circ: 0.934772
Ran for 19550 frames Cell Events: 1071 mean_circ: 0.934847
Ran for 20050 frames Cell Events: 1093 mean_circ: 0.934785
Ran for 22050 frames Cell Events: 1208 mean_circ: 0.935213
Ran for 23550 frames Cell Events: 1279 mean_circ: 0.935253
Ran for 24050 frames Cell Events: 1302 mean_circ: 0.935418
Ran for 24550 frames Cell Events: 1323 mean_circ: 0.93529
Ran for 25050 frames Cell Events: 1363 mean_circ: 0.935355
Ran for 25550 frames Cell Events: 1384 mean_circ: 0.935224
Ran for 26050 frames Cell Events: 1418 mean_circ: 0.935121
Ran for 27050 frames Cell Events: 1464 mean_circ: 0.935202
Ran for 27550 frames Cell Events: 1499 mean_circ: 0.935354
Ran for 28050 frames Cell Events: 1531 mean_circ: 0.935364
Ran for 29050 frames Cell Events: 1606 mean_circ: 0.935431
Ran for 30050 frames Cell Events: 1686 mean_circ: 0.935426
Ran for 31050 frames Cell Events: 1754 mean_circ: 0.935208
Ran for 32050 frames Cell Events: 1830 mean_circ: 0.934522
Ran for 32550 frames Cell Events: 1851 mean_circ: 0.934511
Ran for 34050 frames Cell Events: 1934 mean_circ: 0.93473
Ran for 34550 frames Cell Events: 1967 mean_circ: 0.934734
Ran for 35050 frames Cell Events: 1997 mean_circ: 0.934796
Ran for 36550 frames Cell Events: 2094 mean_circ: 0.934728
Ran for 37550 frames Cell Events: 2177 mean_circ: 0.934666
Ran for 38050 frames Cell Events: 2211 mean_circ: 0.934565
Ran for 39050 frames Cell Events: 2281 mean_circ: 0.934604
Ran for 42050 frames Cell Events: 2461 mean_circ: 0.934817
Ran for 42550 frames Cell Events: 2493 mean_circ: 0.934882
Ran for 43050 frames Cell Events: 2524 mean_circ: 0.934893
Ran for 43550 frames Cell Events: 2543 mean_circ: 0.935011
Ran for 44550 frames Cell Events: 2613 mean_circ: 0.934828
Ran for 45050 frames Cell Events: 2638 mean_circ: 0.9349
Ran for 45550 frames Cell Events: 2669 mean_circ: 0.934882
Ran for 46550 frames Cell Events: 2727 mean_circ: 0.934775
Ran for 48050 frames Cell Events: 2841 mean_circ: 0.93477
Ran for 49050 frames Cell Events: 2918 mean_circ: 0.934636
Ran for 51050 frames Cell Events: 3006 mean_circ: 0.93468
Ran for 51550 frames Cell Events: 3035 mean_circ: 0.934783
Ran for 52050 frames Cell Events: 3058 mean_circ: 0.934776
Ran for 52550 frames Cell Events: 3081 mean_circ: 0.934776
Ran for 53050 frames Cell Events: 3112 mean_circ: 0.934836
Ran for 53550 frames Cell Events: 3154 mean_circ: 0.93481
Ran for 54050 frames Cell Events: 3186 mean_circ: 0.934804
Ran for 54550 frames Cell Events: 3211 mean_circ: 0.934898
Ran for 55550 frames Cell Events: 3291 mean_circ: 0.934991
Ran for 56050 frames Cell Events: 3327 mean_circ: 0.935042
Ran for 56550 frames Cell Events: 3352 mean_circ: 0.935142
Ran for 57050 frames Cell Events: 3375 mean_circ: 0.93517
Ran for 58050 frames Cell Events: 3420 mean_circ: 0.935106
Ran for 61050 frames Cell Events: 3609 mean_circ: 0.934994
Ran for 62050 frames Cell Events: 3673 mean_circ: 0.935079
Ran for 63550 frames Cell Events: 3765 mean_circ: 0.934859
Ran for 64050 frames Cell Events: 3789 mean_circ: 0.93489
Ran for 64550 frames Cell Events: 3806 mean_circ: 0.934909
Ran for 65050 frames Cell Events: 3833 mean_circ: 0.934955
Ran for 67050 frames Cell Events: 3981 mean_circ: 0.934691
Ran for 68050 frames Cell Events: 4047 mean_circ: 0.934771
Ran for 69050 frames Cell Events: 4115 mean_circ: 0.934837
Ran for 70550 frames Cell Events: 4195 mean_circ: 0.934776
Ran for 71050 frames Cell Events: 4220 mean_circ: 0.934792
Ran for 71550 frames Cell Events: 4248 mean_circ: 0.934892
Ran for 72050 frames Cell Events: 4278 mean_circ: 0.934722
Ran for 72550 frames Cell Events: 4308 mean_circ: 0.93474
Ran for 73550 frames Cell Events: 4366 mean_circ: 0.934866
Ran for 74050 frames Cell Events: 4408 mean_circ: 0.934917
Ran for 74550 frames Cell Events: 4440 mean_circ: 0.934741
Ran for 75050 frames Cell Events: 4469 mean_circ: 0.934668
Ran for 75550 frames Cell Events: 4492 mean_circ: 0.934663
Ran for 76050 frames Cell Events: 4530 mean_circ: 0.934616
Ran for 76550 frames Cell Events: 4560 mean_circ: 0.934635
Ran for 77550 frames Cell Events: 4612 mean_circ: 0.93468
Ran for 78050 frames Cell Events: 4639 mean_circ: 0.934628
Ran for 80550 frames Cell Events: 4784 mean_circ: 0.934712
Ran for 81550 frames Cell Events: 4842 mean_circ: 0.934719
Ran for 82050 frames Cell Events: 4870 mean_circ: 0.934706
Ran for 82550 frames Cell Events: 4906 mean_circ: 0.934633
Ran for 83550 frames Cell Events: 4966 mean_circ: 0.934703
Ran for 85050 frames Cell Events: 5074 mean_circ: 0.934701
Ran for 85550 frames Cell Events: 5103 mean_circ: 0.934564
Ran for 86050 frames Cell Events: 5145 mean_circ: 0.934517
Ran for 86550 frames Cell Events: 5165 mean_circ: 0.934455
Ran for 87050 frames Cell Events: 5192 mean_circ: 0.934449
Ran for 87550 frames Cell Events: 5216 mean_circ: 0.934462
Ran for 88550 frames Cell Events: 5279 mean_circ: 0.93448
Ran for 89050 frames Cell Events: 5311 mean_circ: 0.934436
Ran for 89550 frames Cell Events: 5345 mean_circ: 0.934385
Ran for 90550 frames Cell Events: 5399 mean_circ: 0.934302
Ran for 91050 frames Cell Events: 5440 mean_circ: 0.934302
Ran for 91550 frames Cell Events: 5479 mean_circ: 0.934334
Ran for 92050 frames Cell Events: 5505 mean_circ: 0.934293
Ran for 92550 frames Cell Events: 5537 mean_circ: 0.934202
Ran for 93050 frames Cell Events: 5577 mean_circ: 0.934162
Ran for 97050 frames Cell Events: 5857 mean_circ: 0.934038
Ran for 97550 frames Cell Events: 5882 mean_circ: 0.933994
Ran for 98550 frames Cell Events: 5947 mean_circ: 0.933977
Ran for 99050 frames Cell Events: 5985 mean_circ: 0.934058
Ran for 99550 frames Cell Events: 6010 mean_circ: 0.934138
Ran for 100050 frames Cell Events: 6033 mean_circ: 0.934114
Ran for 100550 frames Cell Events: 6063 mean_circ: 0.934193
Ran for 102050 frames Cell Events: 6193 mean_circ: 0.934132
Ran for 102550 frames Cell Events: 6226 mean_circ: 0.934159
Ran for 103050 frames Cell Events: 6266 mean_circ: 0.934159
Ran for 103550 frames Cell Events: 6288 mean_circ: 0.934063
Ran for 104550 frames Cell Events: 6345 mean_circ: 0.934075
Ran for 105050 frames Cell Events: 6378 mean_circ: 0.934059
Ran for 105550 frames Cell Events: 6407 mean_circ: 0.934047
Ran for 106050 frames Cell Events: 6435 mean_circ: 0.934029
Ran for 106550 frames Cell Events: 6475 mean_circ: 0.934027
Ran for 109050 frames Cell Events: 6649 mean_circ: 0.933917
Ran for 109550 frames Cell Events: 6672 mean_circ: 0.933911
Ran for 110050 frames Cell Events: 6712 mean_circ: 0.933835
Ran for 112550 frames Cell Events: 6882 mean_circ: 0.93358
Ran for 114050 frames Cell Events: 6988 mean_circ: 0.933499
Ran for 114550 frames Cell Events: 7027 mean_circ: 0.933481
Ran for 115050 frames Cell Events: 7063 mean_circ: 0.933496
Ran for 116550 frames Cell Events: 7171 mean_circ: 0.933429
Ran for 117050 frames Cell Events: 7198 mean_circ: 0.93344
Ran for 117550 frames Cell Events: 7236 mean_circ: 0.933416
Ran for 118050 frames Cell Events: 7262 mean_circ: 0.933427
Ran for 118550 frames Cell Events: 7286 mean_circ: 0.933437
Ran for 119050 frames Cell Events: 7313 mean_circ: 0.933446
Ran for 119550 frames Cell Events: 7345 mean_circ: 0.933429
Ran for 121550 frames Cell Events: 7493 mean_circ: 0.933448
Ran for 122550 frames Cell Events: 7565 mean_circ: 0.933406
Ran for 124050 frames Cell Events: 7656 mean_circ: 0.933392
Ran for 124550 frames Cell Events: 7679 mean_circ: 0.933383
Ran for 125050 frames Cell Events: 7707 mean_circ: 0.933381
Ran for 125550 frames Cell Events: 7738 mean_circ: 0.933398
Ran for 126050 frames Cell Events: 7766 mean_circ: 0.9334
Ran for 126550 frames Cell Events: 7795 mean_circ: 0.933391
Ran for 127050 frames Cell Events: 7832 mean_circ: 0.933411
Ran for 129550 frames Cell Events: 8005 mean_circ: 0.933465
Ran for 130050 frames Cell Events: 8042 mean_circ: 0.933471
Ran for 130550 frames Cell Events: 8091 mean_circ: 0.933476
Ran for 131550 frames Cell Events: 8190 mean_circ: 0.933366



End this measurment..



Stopping time: 1.84998e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933358
Mean Area: 611.391
Mean FL-1 intensity: 3466.65
Mean FL-2 intensity: 4394.65
Mean FL-3 intensity: 0

frames processed: 131662
Total Time: 66005ms
Number of Runs (new bg img) 0
Frames collected: 131795
Skipped frames: 38059
Rescued Frames: 2068684
Samples DAQ: 32950113
Samples written: 65897364
Samples per Channel: 32948682
FPS: 1996.74

Samples per second: 499184
Expected samples/s: 500000

Events: 8222
Events per second: 124.566
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :195417
# 1 frames :54735
# 2 frames :2739
# 3 frames :1090
# 4 frames :430
# 5 frames :2480
# 6 frames :1119
# 7 frames :965
# 8 frames :514
# 9 frames :536
# 10 frames :475
# 11 frames :268
# 12 frames :294
# 13 frames :196
# 14 frames :202
# 15 frames :133
# 16 frames :114
# 17 frames :96
# 18 frames :94
# 19 frames :60
# 20 frames :58
# 21 frames :40
# 22 frames :27
# 23 frames :15
# 24 frames :16
# 25 frames :14
# 26 frames :16
# 27 frames :15
# 28 frames :10
# 29 frames :4
# 30 frames :6
# 31 frames :4
# 32 frames :8
# 33 frames :7
# 34 frames :2
# 35 frames :4
# 36 frames :2
# 37 frames :1
# 38 frames :1
# 39 frames :4
# 41 frames :3
# 42 frames :2
# 43 frames :1
# 44 frames :2
# 45 frames :1
# 46 frames :1
# 49 frames :2
# 50 frames :1
# 53 frames :1
# 80 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6645
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0