Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.41.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "69f36a8d35296cf221b7c6b70fc6ae1c",
"name": "M1_20170606_1448_dsRNA_N4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:41:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8070 (max 8071) in '8070'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8626
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92395e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 49 mean_circ: 0.936369
Ran for 1550 frames Cell Events: 63 mean_circ: 0.927325
Ran for 2050 frames Cell Events: 72 mean_circ: 0.926895
Ran for 4050 frames Cell Events: 159 mean_circ: 0.922736
Ran for 5050 frames Cell Events: 216 mean_circ: 0.927984
Ran for 5550 frames Cell Events: 235 mean_circ: 0.92952
Ran for 6050 frames Cell Events: 257 mean_circ: 0.929866
Ran for 7050 frames Cell Events: 308 mean_circ: 0.929728
Ran for 8050 frames Cell Events: 360 mean_circ: 0.931174
Ran for 8550 frames Cell Events: 375 mean_circ: 0.930932
Ran for 9050 frames Cell Events: 400 mean_circ: 0.930574
Ran for 10050 frames Cell Events: 450 mean_circ: 0.930557
Ran for 11050 frames Cell Events: 518 mean_circ: 0.929881
Ran for 12050 frames Cell Events: 568 mean_circ: 0.929499
Ran for 12550 frames Cell Events: 599 mean_circ: 0.930138
Ran for 13550 frames Cell Events: 662 mean_circ: 0.929182
Ran for 15550 frames Cell Events: 785 mean_circ: 0.929481
Ran for 16050 frames Cell Events: 812 mean_circ: 0.929499
Ran for 16550 frames Cell Events: 840 mean_circ: 0.929191
Ran for 17050 frames Cell Events: 864 mean_circ: 0.929434
Ran for 17550 frames Cell Events: 887 mean_circ: 0.929778
Ran for 18050 frames Cell Events: 907 mean_circ: 0.929774
Ran for 18550 frames Cell Events: 943 mean_circ: 0.929817
Ran for 19550 frames Cell Events: 1016 mean_circ: 0.929736
Ran for 20050 frames Cell Events: 1039 mean_circ: 0.929289
Ran for 20550 frames Cell Events: 1073 mean_circ: 0.929605
Ran for 21050 frames Cell Events: 1105 mean_circ: 0.929908
Ran for 22550 frames Cell Events: 1207 mean_circ: 0.930228
Ran for 24550 frames Cell Events: 1323 mean_circ: 0.929888
Ran for 25050 frames Cell Events: 1358 mean_circ: 0.93002
Ran for 25550 frames Cell Events: 1385 mean_circ: 0.930289
Ran for 26050 frames Cell Events: 1409 mean_circ: 0.930393
Ran for 26550 frames Cell Events: 1434 mean_circ: 0.93065
Ran for 27050 frames Cell Events: 1460 mean_circ: 0.930747
Ran for 27550 frames Cell Events: 1488 mean_circ: 0.93094
Ran for 28050 frames Cell Events: 1514 mean_circ: 0.931037
Ran for 28550 frames Cell Events: 1560 mean_circ: 0.930873
Ran for 29050 frames Cell Events: 1592 mean_circ: 0.931027
Ran for 29550 frames Cell Events: 1604 mean_circ: 0.930927
Ran for 30550 frames Cell Events: 1662 mean_circ: 0.93126
Ran for 31050 frames Cell Events: 1680 mean_circ: 0.931325
Ran for 32550 frames Cell Events: 1761 mean_circ: 0.931806
Ran for 33050 frames Cell Events: 1784 mean_circ: 0.931611
Ran for 33550 frames Cell Events: 1804 mean_circ: 0.931611
Ran for 34550 frames Cell Events: 1859 mean_circ: 0.931933
Ran for 35550 frames Cell Events: 1904 mean_circ: 0.931672
Ran for 36050 frames Cell Events: 1929 mean_circ: 0.931706
Ran for 36550 frames Cell Events: 1964 mean_circ: 0.931282
Ran for 37550 frames Cell Events: 2037 mean_circ: 0.9313
Ran for 38050 frames Cell Events: 2075 mean_circ: 0.931399
Ran for 39550 frames Cell Events: 2149 mean_circ: 0.93141
Ran for 40050 frames Cell Events: 2181 mean_circ: 0.931544
Ran for 40550 frames Cell Events: 2217 mean_circ: 0.931198
Ran for 41050 frames Cell Events: 2229 mean_circ: 0.931039
Ran for 41550 frames Cell Events: 2251 mean_circ: 0.931152
Ran for 46050 frames Cell Events: 2576 mean_circ: 0.931504
Ran for 46550 frames Cell Events: 2608 mean_circ: 0.931444
Ran for 47550 frames Cell Events: 2672 mean_circ: 0.931496
Ran for 49550 frames Cell Events: 2795 mean_circ: 0.931315
Ran for 50050 frames Cell Events: 2822 mean_circ: 0.931394
Ran for 50550 frames Cell Events: 2846 mean_circ: 0.931163
Ran for 51050 frames Cell Events: 2868 mean_circ: 0.931166
Ran for 51550 frames Cell Events: 2893 mean_circ: 0.93103
Ran for 52050 frames Cell Events: 2919 mean_circ: 0.930891
Ran for 52550 frames Cell Events: 2946 mean_circ: 0.930812
Ran for 53050 frames Cell Events: 2958 mean_circ: 0.930781
Ran for 54550 frames Cell Events: 3045 mean_circ: 0.930829
Ran for 55050 frames Cell Events: 3064 mean_circ: 0.930857
Ran for 55550 frames Cell Events: 3081 mean_circ: 0.930988
Ran for 56050 frames Cell Events: 3096 mean_circ: 0.930996
Ran for 56550 frames Cell Events: 3128 mean_circ: 0.930979
Ran for 57050 frames Cell Events: 3153 mean_circ: 0.930938
Ran for 58550 frames Cell Events: 3242 mean_circ: 0.931281
Ran for 59550 frames Cell Events: 3322 mean_circ: 0.931351
Ran for 61550 frames Cell Events: 3457 mean_circ: 0.931539
Ran for 62050 frames Cell Events: 3490 mean_circ: 0.931453
Ran for 63050 frames Cell Events: 3552 mean_circ: 0.931593
Ran for 63550 frames Cell Events: 3596 mean_circ: 0.931697
Ran for 64550 frames Cell Events: 3672 mean_circ: 0.931651
Ran for 65050 frames Cell Events: 3701 mean_circ: 0.931595
Ran for 65550 frames Cell Events: 3738 mean_circ: 0.931595
Ran for 66050 frames Cell Events: 3773 mean_circ: 0.931563
Ran for 68050 frames Cell Events: 3913 mean_circ: 0.931414
Ran for 69050 frames Cell Events: 3968 mean_circ: 0.931277
Ran for 70550 frames Cell Events: 4046 mean_circ: 0.931297
Ran for 71050 frames Cell Events: 4074 mean_circ: 0.93127
Ran for 71550 frames Cell Events: 4103 mean_circ: 0.931272
Ran for 72550 frames Cell Events: 4151 mean_circ: 0.931293
Ran for 73550 frames Cell Events: 4200 mean_circ: 0.931005
Ran for 74550 frames Cell Events: 4280 mean_circ: 0.931161
Ran for 76050 frames Cell Events: 4387 mean_circ: 0.931033
Ran for 76550 frames Cell Events: 4412 mean_circ: 0.930985
Ran for 77550 frames Cell Events: 4471 mean_circ: 0.931081
Ran for 78050 frames Cell Events: 4498 mean_circ: 0.931046
Ran for 80050 frames Cell Events: 4615 mean_circ: 0.930943
Ran for 81050 frames Cell Events: 4678 mean_circ: 0.930897
Ran for 81550 frames Cell Events: 4706 mean_circ: 0.930865
Ran for 82050 frames Cell Events: 4731 mean_circ: 0.9309
Ran for 83050 frames Cell Events: 4783 mean_circ: 0.93085
Ran for 83550 frames Cell Events: 4807 mean_circ: 0.930735
Ran for 84550 frames Cell Events: 4855 mean_circ: 0.930748
Ran for 86050 frames Cell Events: 4931 mean_circ: 0.930759
Ran for 87550 frames Cell Events: 5001 mean_circ: 0.93064
Ran for 88050 frames Cell Events: 5033 mean_circ: 0.930636
Ran for 88550 frames Cell Events: 5065 mean_circ: 0.930628
Ran for 89550 frames Cell Events: 5130 mean_circ: 0.93068
Ran for 92050 frames Cell Events: 5272 mean_circ: 0.930722
Ran for 93050 frames Cell Events: 5330 mean_circ: 0.930691
Ran for 95050 frames Cell Events: 5459 mean_circ: 0.93062
Ran for 95550 frames Cell Events: 5482 mean_circ: 0.930686
Ran for 96050 frames Cell Events: 5510 mean_circ: 0.930687
Ran for 96550 frames Cell Events: 5539 mean_circ: 0.930717
Ran for 98050 frames Cell Events: 5637 mean_circ: 0.930836
Ran for 100050 frames Cell Events: 5766 mean_circ: 0.930637
Ran for 101050 frames Cell Events: 5811 mean_circ: 0.930703
Ran for 101550 frames Cell Events: 5839 mean_circ: 0.930691
Ran for 104550 frames Cell Events: 6004 mean_circ: 0.930903
Ran for 105050 frames Cell Events: 6023 mean_circ: 0.930927
Ran for 105550 frames Cell Events: 6048 mean_circ: 0.930975
Ran for 106050 frames Cell Events: 6074 mean_circ: 0.930977
Ran for 106550 frames Cell Events: 6107 mean_circ: 0.930896
Ran for 107050 frames Cell Events: 6140 mean_circ: 0.93094
Ran for 108050 frames Cell Events: 6188 mean_circ: 0.930935
Ran for 108550 frames Cell Events: 6218 mean_circ: 0.930955
Ran for 109050 frames Cell Events: 6244 mean_circ: 0.930926
Ran for 109550 frames Cell Events: 6258 mean_circ: 0.930907
Ran for 110550 frames Cell Events: 6308 mean_circ: 0.93095
Ran for 113550 frames Cell Events: 6466 mean_circ: 0.930748
Ran for 114550 frames Cell Events: 6518 mean_circ: 0.930562
Ran for 117050 frames Cell Events: 6675 mean_circ: 0.930563
Ran for 118050 frames Cell Events: 6718 mean_circ: 0.930513
Ran for 118550 frames Cell Events: 6745 mean_circ: 0.930527
Ran for 119550 frames Cell Events: 6807 mean_circ: 0.930539
Ran for 120550 frames Cell Events: 6876 mean_circ: 0.93047
Ran for 121050 frames Cell Events: 6917 mean_circ: 0.930456
Ran for 121550 frames Cell Events: 6947 mean_circ: 0.930362
Ran for 122050 frames Cell Events: 6977 mean_circ: 0.930425
Ran for 122550 frames Cell Events: 7009 mean_circ: 0.930427
Ran for 123050 frames Cell Events: 7032 mean_circ: 0.930429
Ran for 124050 frames Cell Events: 7074 mean_circ: 0.930413
Ran for 125050 frames Cell Events: 7134 mean_circ: 0.930391
Ran for 126050 frames Cell Events: 7205 mean_circ: 0.930235
Ran for 127050 frames Cell Events: 7266 mean_circ: 0.930292
Ran for 128050 frames Cell Events: 7334 mean_circ: 0.930247
Ran for 129050 frames Cell Events: 7403 mean_circ: 0.930279
Ran for 129550 frames Cell Events: 7427 mean_circ: 0.930317
Ran for 130050 frames Cell Events: 7459 mean_circ: 0.930335
Ran for 131050 frames Cell Events: 7516 mean_circ: 0.930326
Ran for 132550 frames Cell Events: 7600 mean_circ: 0.930367
Ran for 134050 frames Cell Events: 7674 mean_circ: 0.93028
Ran for 134550 frames Cell Events: 7687 mean_circ: 0.930324
Ran for 136050 frames Cell Events: 7766 mean_circ: 0.930364
Ran for 136550 frames Cell Events: 7788 mean_circ: 0.930337
Ran for 137550 frames Cell Events: 7846 mean_circ: 0.930287
Ran for 138050 frames Cell Events: 7875 mean_circ: 0.930322
Ran for 138550 frames Cell Events: 7904 mean_circ: 0.930328
Ran for 139050 frames Cell Events: 7928 mean_circ: 0.930302



End this measurment..



Stopping time: 1.92402e+009
****************************************
Measurement finished

Mean Circularitiy: 0.930204
Mean Area: 657.687
Mean FL-1 intensity: 3875.09
Mean FL-2 intensity: 5155.49
Mean FL-3 intensity: 0

frames processed: 139801
Total Time: 70700.7ms
Number of Runs (new bg img) 0
Frames collected: 141226
Skipped frames: 36200
Rescued Frames: 984019
Samples DAQ: 35307287
Samples written: 70612854
Samples per Channel: 35306427
FPS: 1997.52

Samples per second: 499379
Expected samples/s: 500000

Events: 8071
Events per second: 114.157
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :215902
# 1 frames :54450
# 2 frames :2668
# 3 frames :1031
# 4 frames :486
# 5 frames :239
# 6 frames :2082
# 7 frames :1027
# 8 frames :1023
# 9 frames :556
# 10 frames :384
# 11 frames :373
# 12 frames :293
# 13 frames :200
# 14 frames :237
# 15 frames :183
# 16 frames :152
# 17 frames :144
# 18 frames :97
# 19 frames :68
# 20 frames :77
# 21 frames :67
# 22 frames :56
# 23 frames :47
# 24 frames :31
# 25 frames :29
# 26 frames :37
# 27 frames :19
# 28 frames :18
# 29 frames :16
# 30 frames :11
# 31 frames :14
# 32 frames :6
# 33 frames :8
# 34 frames :11
# 35 frames :11
# 36 frames :6
# 37 frames :6
# 38 frames :3
# 39 frames :9
# 40 frames :1
# 41 frames :6
# 42 frames :1
# 43 frames :3
# 44 frames :2
# 45 frames :4
# 47 frames :1
# 48 frames :1
# 51 frames :2
# 52 frames :1
# 54 frames :1
# 56 frames :1
# 61 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8626
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0