Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.43.05 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e551319458276fda99d021da3338f6f5",
"name": "M1_20170606_1510_dsRNA_N6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:43:25"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8082 (max 8083) in '8082'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9855
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92522e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 4050 frames Cell Events: 80 mean_circ: 0.94701
Ran for 4550 frames Cell Events: 89 mean_circ: 0.94701
Ran for 5050 frames Cell Events: 126 mean_circ: 0.94701
Ran for 5550 frames Cell Events: 140 mean_circ: 0.950509
Ran for 7050 frames Cell Events: 159 mean_circ: 0.951226
Ran for 9550 frames Cell Events: 193 mean_circ: 0.95194
Ran for 12550 frames Cell Events: 264 mean_circ: 0.952084
Ran for 17050 frames Cell Events: 349 mean_circ: 0.950245
Ran for 20550 frames Cell Events: 494 mean_circ: 0.950693
Ran for 21550 frames Cell Events: 546 mean_circ: 0.950735
Ran for 23050 frames Cell Events: 637 mean_circ: 0.951228
Ran for 24050 frames Cell Events: 700 mean_circ: 0.950921
Ran for 26550 frames Cell Events: 842 mean_circ: 0.95087
Ran for 28050 frames Cell Events: 924 mean_circ: 0.951247
Ran for 29550 frames Cell Events: 1012 mean_circ: 0.950854
Ran for 31050 frames Cell Events: 1093 mean_circ: 0.951236
Ran for 32050 frames Cell Events: 1153 mean_circ: 0.951233
Ran for 33050 frames Cell Events: 1197 mean_circ: 0.95107
Ran for 35050 frames Cell Events: 1309 mean_circ: 0.951397
Ran for 36050 frames Cell Events: 1365 mean_circ: 0.951322
Ran for 37050 frames Cell Events: 1417 mean_circ: 0.951515
Ran for 38050 frames Cell Events: 1485 mean_circ: 0.951617
Ran for 39050 frames Cell Events: 1539 mean_circ: 0.951682
Ran for 40050 frames Cell Events: 1602 mean_circ: 0.951917
Ran for 41050 frames Cell Events: 1647 mean_circ: 0.951804
Ran for 43050 frames Cell Events: 1783 mean_circ: 0.951844
Ran for 43550 frames Cell Events: 1819 mean_circ: 0.951717
Ran for 44050 frames Cell Events: 1843 mean_circ: 0.951735
Ran for 45050 frames Cell Events: 1909 mean_circ: 0.951639
Ran for 46050 frames Cell Events: 1972 mean_circ: 0.951723
Ran for 48050 frames Cell Events: 2103 mean_circ: 0.951449
Ran for 48550 frames Cell Events: 2123 mean_circ: 0.951442
Ran for 49550 frames Cell Events: 2170 mean_circ: 0.951591
Ran for 51050 frames Cell Events: 2276 mean_circ: 0.951471
Ran for 51550 frames Cell Events: 2315 mean_circ: 0.951219
Ran for 52050 frames Cell Events: 2345 mean_circ: 0.95114
Ran for 53550 frames Cell Events: 2433 mean_circ: 0.951297
Ran for 57050 frames Cell Events: 2660 mean_circ: 0.951442
Ran for 58050 frames Cell Events: 2726 mean_circ: 0.951359
Ran for 60050 frames Cell Events: 2840 mean_circ: 0.950996
Ran for 60550 frames Cell Events: 2878 mean_circ: 0.950959
Ran for 61050 frames Cell Events: 2903 mean_circ: 0.950992
Ran for 61550 frames Cell Events: 2945 mean_circ: 0.950965
Ran for 62050 frames Cell Events: 2981 mean_circ: 0.950979
Ran for 65550 frames Cell Events: 3244 mean_circ: 0.950923
Ran for 66050 frames Cell Events: 3269 mean_circ: 0.95101
Ran for 67050 frames Cell Events: 3320 mean_circ: 0.95105
Ran for 68050 frames Cell Events: 3387 mean_circ: 0.95097
Ran for 70050 frames Cell Events: 3500 mean_circ: 0.95106
Ran for 71550 frames Cell Events: 3607 mean_circ: 0.95104
Ran for 72550 frames Cell Events: 3655 mean_circ: 0.95104
Ran for 73050 frames Cell Events: 3685 mean_circ: 0.951074
Ran for 74050 frames Cell Events: 3759 mean_circ: 0.951132
Ran for 75050 frames Cell Events: 3828 mean_circ: 0.951187
Ran for 76550 frames Cell Events: 3924 mean_circ: 0.951163
Ran for 78050 frames Cell Events: 4001 mean_circ: 0.951177
Ran for 79050 frames Cell Events: 4040 mean_circ: 0.951195
Ran for 80050 frames Cell Events: 4100 mean_circ: 0.951239
Ran for 80550 frames Cell Events: 4136 mean_circ: 0.951218
Ran for 81550 frames Cell Events: 4179 mean_circ: 0.95128
Ran for 83050 frames Cell Events: 4288 mean_circ: 0.951198
Ran for 83550 frames Cell Events: 4305 mean_circ: 0.951155
Ran for 86050 frames Cell Events: 4461 mean_circ: 0.951097
Ran for 86550 frames Cell Events: 4485 mean_circ: 0.951084
Ran for 88050 frames Cell Events: 4580 mean_circ: 0.951023
Ran for 88550 frames Cell Events: 4603 mean_circ: 0.951077
Ran for 89550 frames Cell Events: 4660 mean_circ: 0.951118
Ran for 91550 frames Cell Events: 4786 mean_circ: 0.951034
Ran for 92550 frames Cell Events: 4874 mean_circ: 0.951081
Ran for 93050 frames Cell Events: 4900 mean_circ: 0.951131
Ran for 93550 frames Cell Events: 4922 mean_circ: 0.951163
Ran for 95550 frames Cell Events: 5033 mean_circ: 0.951131
Ran for 96050 frames Cell Events: 5058 mean_circ: 0.951131
Ran for 96550 frames Cell Events: 5092 mean_circ: 0.951131
Ran for 97050 frames Cell Events: 5105 mean_circ: 0.951136
Ran for 97550 frames Cell Events: 5121 mean_circ: 0.951119
Ran for 99050 frames Cell Events: 5170 mean_circ: 0.951047
Ran for 101550 frames Cell Events: 5342 mean_circ: 0.951132
Ran for 102550 frames Cell Events: 5413 mean_circ: 0.951194
Ran for 104550 frames Cell Events: 5549 mean_circ: 0.951178
Ran for 105050 frames Cell Events: 5569 mean_circ: 0.951152
Ran for 106050 frames Cell Events: 5648 mean_circ: 0.951113
Ran for 106550 frames Cell Events: 5674 mean_circ: 0.950996
Ran for 108550 frames Cell Events: 5781 mean_circ: 0.950911
Ran for 110050 frames Cell Events: 5868 mean_circ: 0.950852
Ran for 110550 frames Cell Events: 5907 mean_circ: 0.950852
Ran for 111550 frames Cell Events: 5968 mean_circ: 0.950887
Ran for 112050 frames Cell Events: 5997 mean_circ: 0.950916
Ran for 112550 frames Cell Events: 6026 mean_circ: 0.950926
Ran for 113550 frames Cell Events: 6079 mean_circ: 0.950895
Ran for 114550 frames Cell Events: 6159 mean_circ: 0.950832
Ran for 115050 frames Cell Events: 6180 mean_circ: 0.950879
Ran for 115550 frames Cell Events: 6208 mean_circ: 0.950828
Ran for 116050 frames Cell Events: 6230 mean_circ: 0.950863
Ran for 116550 frames Cell Events: 6265 mean_circ: 0.95089
Ran for 118050 frames Cell Events: 6363 mean_circ: 0.950936
Ran for 119050 frames Cell Events: 6435 mean_circ: 0.950893
Ran for 119550 frames Cell Events: 6460 mean_circ: 0.950933
Ran for 120550 frames Cell Events: 6521 mean_circ: 0.950974
Ran for 121050 frames Cell Events: 6561 mean_circ: 0.950974
Ran for 121550 frames Cell Events: 6598 mean_circ: 0.95098
Ran for 126050 frames Cell Events: 6884 mean_circ: 0.951124
Ran for 127050 frames Cell Events: 6954 mean_circ: 0.951104
Ran for 127550 frames Cell Events: 6990 mean_circ: 0.951113
Ran for 128550 frames Cell Events: 7068 mean_circ: 0.951022
Ran for 130050 frames Cell Events: 7159 mean_circ: 0.95099
Ran for 130550 frames Cell Events: 7191 mean_circ: 0.950993
Ran for 131050 frames Cell Events: 7219 mean_circ: 0.951021
Ran for 131550 frames Cell Events: 7243 mean_circ: 0.951048
Ran for 132550 frames Cell Events: 7299 mean_circ: 0.951009
Ran for 134550 frames Cell Events: 7416 mean_circ: 0.951006
Ran for 136050 frames Cell Events: 7502 mean_circ: 0.951041
Ran for 137050 frames Cell Events: 7564 mean_circ: 0.951075
Ran for 137550 frames Cell Events: 7609 mean_circ: 0.951075
Ran for 138550 frames Cell Events: 7667 mean_circ: 0.951062
Ran for 141050 frames Cell Events: 7833 mean_circ: 0.951112
Ran for 141550 frames Cell Events: 7869 mean_circ: 0.951089
Ran for 142050 frames Cell Events: 7908 mean_circ: 0.951074
Ran for 144050 frames Cell Events: 8037 mean_circ: 0.951053



End this measurment..



Stopping time: 1.9253e+009
****************************************
Measurement finished

Mean Circularitiy: 0.951013
Mean Area: 621.67
Mean FL-1 intensity: 3769.04
Mean FL-2 intensity: 4561.59
Mean FL-3 intensity: 0

frames processed: 130678
Total Time: 72452.4ms
Number of Runs (new bg img) 0
Frames collected: 144761
Skipped frames: 52732
Rescued Frames: 1241933
Samples DAQ: 36190291
Samples written: 72380496
Samples per Channel: 36190248
FPS: 1998.02

Samples per second: 499504
Expected samples/s: 500000

Events: 8083
Events per second: 111.563
jumped back (PMT): 3215
#Frames read, frequency
# 0 frames :212083
# 1 frames :44709
# 2 frames :2770
# 3 frames :884
# 4 frames :539
# 5 frames :245
# 6 frames :1236
# 7 frames :1210
# 8 frames :1008
# 9 frames :587
# 10 frames :496
# 11 frames :301
# 12 frames :220
# 13 frames :145
# 14 frames :212
# 15 frames :213
# 16 frames :182
# 17 frames :140
# 18 frames :82
# 19 frames :75
# 20 frames :66
# 21 frames :79
# 22 frames :61
# 23 frames :48
# 24 frames :59
# 25 frames :45
# 26 frames :29
# 27 frames :44
# 28 frames :28
# 29 frames :31
# 30 frames :22
# 31 frames :24
# 32 frames :27
# 33 frames :14
# 34 frames :4
# 35 frames :11
# 36 frames :11
# 37 frames :10
# 38 frames :11
# 39 frames :6
# 40 frames :4
# 41 frames :2
# 42 frames :6
# 43 frames :2
# 44 frames :6
# 45 frames :2
# 46 frames :1
# 47 frames :7
# 48 frames :3
# 49 frames :2
# 50 frames :4
# 51 frames :2
# 52 frames :7
# 53 frames :1
# 54 frames :3
# 55 frames :1
# 56 frames :2
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 60 frames :1
# 61 frames :1
# 62 frames :3
# 65 frames :1
# 68 frames :1
# 69 frames :1
# 70 frames :1
# 74 frames :2
# 75 frames :1
# 76 frames :2
# 78 frames :1
# 81 frames :2
# 82 frames :1
# 83 frames :1
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# 85 frames :1
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# 88 frames :3
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# 94 frames :4
# 95 frames :3
# 98 frames :2
# 99 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9855
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0