Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.43.32 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e30470552426c7f0541f28aea3897395",
"name": "M1_20170606_1512_dsRNA_N6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:43:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8865 (max 8866) in '8865'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.966
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92539e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.949217
Ran for 1550 frames Cell Events: 103 mean_circ: 0.946487
Ran for 3050 frames Cell Events: 233 mean_circ: 0.944175
Ran for 6050 frames Cell Events: 467 mean_circ: 0.939609
Ran for 6550 frames Cell Events: 511 mean_circ: 0.940511
Ran for 8550 frames Cell Events: 627 mean_circ: 0.940832
Ran for 11050 frames Cell Events: 808 mean_circ: 0.940537
Ran for 12050 frames Cell Events: 876 mean_circ: 0.940688
Ran for 17050 frames Cell Events: 1272 mean_circ: 0.940221
Ran for 18050 frames Cell Events: 1340 mean_circ: 0.940085
Ran for 18550 frames Cell Events: 1375 mean_circ: 0.939956
Ran for 19050 frames Cell Events: 1406 mean_circ: 0.93997
Ran for 21050 frames Cell Events: 1524 mean_circ: 0.939974
Ran for 22550 frames Cell Events: 1623 mean_circ: 0.939698
Ran for 23550 frames Cell Events: 1681 mean_circ: 0.939713
Ran for 24050 frames Cell Events: 1713 mean_circ: 0.939503
Ran for 26050 frames Cell Events: 1832 mean_circ: 0.939296
Ran for 26550 frames Cell Events: 1865 mean_circ: 0.939277
Ran for 27550 frames Cell Events: 1933 mean_circ: 0.93936
Ran for 28550 frames Cell Events: 2007 mean_circ: 0.939227
Ran for 29050 frames Cell Events: 2043 mean_circ: 0.939254
Ran for 29550 frames Cell Events: 2074 mean_circ: 0.939307
Ran for 33050 frames Cell Events: 2336 mean_circ: 0.938843
Ran for 33550 frames Cell Events: 2362 mean_circ: 0.938861
Ran for 34050 frames Cell Events: 2397 mean_circ: 0.938952
Ran for 34550 frames Cell Events: 2432 mean_circ: 0.939019
Ran for 35550 frames Cell Events: 2496 mean_circ: 0.939062
Ran for 36050 frames Cell Events: 2532 mean_circ: 0.939193
Ran for 36550 frames Cell Events: 2561 mean_circ: 0.939181
Ran for 37050 frames Cell Events: 2596 mean_circ: 0.939181
Ran for 39050 frames Cell Events: 2749 mean_circ: 0.93939
Ran for 41550 frames Cell Events: 2882 mean_circ: 0.939142
Ran for 42550 frames Cell Events: 2949 mean_circ: 0.938977
Ran for 43050 frames Cell Events: 2984 mean_circ: 0.939013
Ran for 44050 frames Cell Events: 3064 mean_circ: 0.938935
Ran for 44550 frames Cell Events: 3104 mean_circ: 0.93903
Ran for 46550 frames Cell Events: 3250 mean_circ: 0.939072
Ran for 47050 frames Cell Events: 3272 mean_circ: 0.93908
Ran for 47550 frames Cell Events: 3298 mean_circ: 0.939085
Ran for 48050 frames Cell Events: 3329 mean_circ: 0.939142
Ran for 48550 frames Cell Events: 3354 mean_circ: 0.939118
Ran for 49550 frames Cell Events: 3413 mean_circ: 0.939006
Ran for 51050 frames Cell Events: 3524 mean_circ: 0.938862
Ran for 52050 frames Cell Events: 3586 mean_circ: 0.93897
Ran for 52550 frames Cell Events: 3619 mean_circ: 0.938985
Ran for 53550 frames Cell Events: 3685 mean_circ: 0.938858
Ran for 54050 frames Cell Events: 3711 mean_circ: 0.938878
Ran for 55050 frames Cell Events: 3773 mean_circ: 0.938744
Ran for 55550 frames Cell Events: 3810 mean_circ: 0.938706
Ran for 56550 frames Cell Events: 3880 mean_circ: 0.938701
Ran for 57550 frames Cell Events: 3974 mean_circ: 0.938661
Ran for 58550 frames Cell Events: 4041 mean_circ: 0.93873
Ran for 59050 frames Cell Events: 4084 mean_circ: 0.938746
Ran for 60550 frames Cell Events: 4203 mean_circ: 0.938775
Ran for 63050 frames Cell Events: 4379 mean_circ: 0.938646
Ran for 64050 frames Cell Events: 4442 mean_circ: 0.938569
Ran for 65050 frames Cell Events: 4517 mean_circ: 0.938516
Ran for 66550 frames Cell Events: 4609 mean_circ: 0.938508
Ran for 67550 frames Cell Events: 4669 mean_circ: 0.938554
Ran for 68550 frames Cell Events: 4722 mean_circ: 0.938545
Ran for 70550 frames Cell Events: 4844 mean_circ: 0.938454
Ran for 71050 frames Cell Events: 4866 mean_circ: 0.938509
Ran for 73550 frames Cell Events: 5039 mean_circ: 0.938481
Ran for 74050 frames Cell Events: 5070 mean_circ: 0.938482
Ran for 74550 frames Cell Events: 5106 mean_circ: 0.938404
Ran for 75050 frames Cell Events: 5153 mean_circ: 0.938404
Ran for 77550 frames Cell Events: 5339 mean_circ: 0.938431
Ran for 78050 frames Cell Events: 5373 mean_circ: 0.938453
Ran for 79050 frames Cell Events: 5436 mean_circ: 0.938533
Ran for 80550 frames Cell Events: 5542 mean_circ: 0.938537
Ran for 81550 frames Cell Events: 5593 mean_circ: 0.938539
Ran for 82050 frames Cell Events: 5615 mean_circ: 0.938521
Ran for 82550 frames Cell Events: 5646 mean_circ: 0.938551
Ran for 84050 frames Cell Events: 5724 mean_circ: 0.938564
Ran for 84550 frames Cell Events: 5761 mean_circ: 0.938518
Ran for 88050 frames Cell Events: 5985 mean_circ: 0.938386
Ran for 88550 frames Cell Events: 6016 mean_circ: 0.938421
Ran for 90050 frames Cell Events: 6126 mean_circ: 0.938361
Ran for 90550 frames Cell Events: 6159 mean_circ: 0.938294
Ran for 94050 frames Cell Events: 6451 mean_circ: 0.93817
Ran for 95050 frames Cell Events: 6513 mean_circ: 0.938112
Ran for 98550 frames Cell Events: 6761 mean_circ: 0.938072
Ran for 100550 frames Cell Events: 6907 mean_circ: 0.937954
Ran for 101050 frames Cell Events: 6943 mean_circ: 0.937959
Ran for 103050 frames Cell Events: 7084 mean_circ: 0.937979
Ran for 105050 frames Cell Events: 7204 mean_circ: 0.93787
Ran for 105550 frames Cell Events: 7231 mean_circ: 0.937879
Ran for 107050 frames Cell Events: 7360 mean_circ: 0.937907
Ran for 110050 frames Cell Events: 7603 mean_circ: 0.937722
Ran for 111550 frames Cell Events: 7694 mean_circ: 0.937734
Ran for 113050 frames Cell Events: 7770 mean_circ: 0.937799
Ran for 114550 frames Cell Events: 7871 mean_circ: 0.937812
Ran for 116050 frames Cell Events: 7975 mean_circ: 0.937776
Ran for 117050 frames Cell Events: 8029 mean_circ: 0.937833
Ran for 119050 frames Cell Events: 8178 mean_circ: 0.937775
Ran for 120550 frames Cell Events: 8265 mean_circ: 0.937756
Ran for 121050 frames Cell Events: 8296 mean_circ: 0.937737
Ran for 122050 frames Cell Events: 8366 mean_circ: 0.937699
Ran for 124050 frames Cell Events: 8495 mean_circ: 0.937638
Ran for 126050 frames Cell Events: 8616 mean_circ: 0.937649
Ran for 126550 frames Cell Events: 8644 mean_circ: 0.937655
Ran for 128550 frames Cell Events: 8752 mean_circ: 0.937627
Ran for 129050 frames Cell Events: 8794 mean_circ: 0.937659
Ran for 129550 frames Cell Events: 8834 mean_circ: 0.937651



End this measurment..



Stopping time: 1.92545e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937662
Mean Area: 623.378
Mean FL-1 intensity: 3546.6
Mean FL-2 intensity: 4893.65
Mean FL-3 intensity: 0

frames processed: 128122
Total Time: 65130.4ms
Number of Runs (new bg img) 0
Frames collected: 130121
Skipped frames: 54731
Rescued Frames: 1321797
Samples DAQ: 32530506
Samples written: 65060444
Samples per Channel: 32530222
FPS: 1997.85

Samples per second: 499463
Expected samples/s: 500000

Events: 8866
Events per second: 136.127
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :211877
# 1 frames :37107
# 2 frames :2349
# 3 frames :858
# 4 frames :474
# 5 frames :246
# 6 frames :1804
# 7 frames :936
# 8 frames :938
# 9 frames :506
# 10 frames :389
# 11 frames :392
# 12 frames :249
# 13 frames :184
# 14 frames :274
# 15 frames :214
# 16 frames :200
# 17 frames :158
# 18 frames :125
# 19 frames :105
# 20 frames :84
# 21 frames :72
# 22 frames :72
# 23 frames :61
# 24 frames :58
# 25 frames :44
# 26 frames :37
# 27 frames :35
# 28 frames :37
# 29 frames :26
# 30 frames :30
# 31 frames :33
# 32 frames :20
# 33 frames :14
# 34 frames :14
# 35 frames :18
# 36 frames :16
# 37 frames :6
# 38 frames :6
# 39 frames :16
# 40 frames :4
# 41 frames :6
# 42 frames :8
# 43 frames :4
# 44 frames :5
# 45 frames :2
# 46 frames :7
# 47 frames :3
# 48 frames :5
# 49 frames :2
# 50 frames :5
# 51 frames :3
# 52 frames :3
# 53 frames :3
# 54 frames :1
# 56 frames :3
# 57 frames :1
# 59 frames :1
# 61 frames :2
# 63 frames :1
# 67 frames :3
# 73 frames :1
# 76 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.966
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0