Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.45.21 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fc3d3a776fde575e142ca09c93c2cc1d",
"name": "M1_20170606_1538_dsRNA_N8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:45:41"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8160 (max 8161) in '8160'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0443
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92691e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.947198
Ran for 1050 frames Cell Events: 53 mean_circ: 0.930201
Ran for 2050 frames Cell Events: 109 mean_circ: 0.933437
Ran for 3050 frames Cell Events: 158 mean_circ: 0.936066
Ran for 6050 frames Cell Events: 356 mean_circ: 0.936039
Ran for 6550 frames Cell Events: 384 mean_circ: 0.936387
Ran for 8050 frames Cell Events: 490 mean_circ: 0.936992
Ran for 8550 frames Cell Events: 523 mean_circ: 0.936919
Ran for 9050 frames Cell Events: 542 mean_circ: 0.936084
Ran for 9550 frames Cell Events: 568 mean_circ: 0.936338
Ran for 10550 frames Cell Events: 626 mean_circ: 0.936434
Ran for 11050 frames Cell Events: 660 mean_circ: 0.936687
Ran for 11550 frames Cell Events: 692 mean_circ: 0.93673
Ran for 12050 frames Cell Events: 712 mean_circ: 0.936728
Ran for 13050 frames Cell Events: 784 mean_circ: 0.935593
Ran for 13550 frames Cell Events: 816 mean_circ: 0.93526
Ran for 14550 frames Cell Events: 865 mean_circ: 0.935288
Ran for 18550 frames Cell Events: 1151 mean_circ: 0.935301
Ran for 20550 frames Cell Events: 1252 mean_circ: 0.935798
Ran for 21550 frames Cell Events: 1308 mean_circ: 0.935939
Ran for 22050 frames Cell Events: 1349 mean_circ: 0.936001
Ran for 23550 frames Cell Events: 1438 mean_circ: 0.9356
Ran for 24050 frames Cell Events: 1460 mean_circ: 0.935828
Ran for 24550 frames Cell Events: 1488 mean_circ: 0.935696
Ran for 25050 frames Cell Events: 1510 mean_circ: 0.935812
Ran for 25550 frames Cell Events: 1544 mean_circ: 0.935799
Ran for 26050 frames Cell Events: 1581 mean_circ: 0.935633
Ran for 27050 frames Cell Events: 1658 mean_circ: 0.935201
Ran for 28050 frames Cell Events: 1715 mean_circ: 0.935666
Ran for 30050 frames Cell Events: 1814 mean_circ: 0.935909
Ran for 31050 frames Cell Events: 1866 mean_circ: 0.936057
Ran for 32550 frames Cell Events: 1971 mean_circ: 0.936579
Ran for 33050 frames Cell Events: 1996 mean_circ: 0.936593
Ran for 34050 frames Cell Events: 2052 mean_circ: 0.936174
Ran for 35050 frames Cell Events: 2087 mean_circ: 0.936423
Ran for 36050 frames Cell Events: 2147 mean_circ: 0.936623
Ran for 36550 frames Cell Events: 2174 mean_circ: 0.936626
Ran for 37050 frames Cell Events: 2204 mean_circ: 0.936721
Ran for 37550 frames Cell Events: 2219 mean_circ: 0.93682
Ran for 38050 frames Cell Events: 2252 mean_circ: 0.936797
Ran for 38550 frames Cell Events: 2280 mean_circ: 0.936802
Ran for 39050 frames Cell Events: 2307 mean_circ: 0.936612
Ran for 40050 frames Cell Events: 2358 mean_circ: 0.936877
Ran for 40550 frames Cell Events: 2379 mean_circ: 0.936969
Ran for 41550 frames Cell Events: 2429 mean_circ: 0.936851
Ran for 42050 frames Cell Events: 2448 mean_circ: 0.93674
Ran for 42550 frames Cell Events: 2474 mean_circ: 0.936832
Ran for 43550 frames Cell Events: 2545 mean_circ: 0.936396
Ran for 44050 frames Cell Events: 2579 mean_circ: 0.936454
Ran for 45050 frames Cell Events: 2641 mean_circ: 0.93675
Ran for 46050 frames Cell Events: 2710 mean_circ: 0.936772
Ran for 46550 frames Cell Events: 2743 mean_circ: 0.936572
Ran for 47050 frames Cell Events: 2771 mean_circ: 0.936536
Ran for 47550 frames Cell Events: 2795 mean_circ: 0.936377
Ran for 48050 frames Cell Events: 2815 mean_circ: 0.936463
Ran for 48550 frames Cell Events: 2837 mean_circ: 0.93644
Ran for 50050 frames Cell Events: 2916 mean_circ: 0.936723
Ran for 50550 frames Cell Events: 2948 mean_circ: 0.936674
Ran for 52050 frames Cell Events: 3052 mean_circ: 0.93655
Ran for 52550 frames Cell Events: 3086 mean_circ: 0.936516
Ran for 53050 frames Cell Events: 3117 mean_circ: 0.936471
Ran for 53550 frames Cell Events: 3147 mean_circ: 0.936517
Ran for 54050 frames Cell Events: 3175 mean_circ: 0.936498
Ran for 54550 frames Cell Events: 3207 mean_circ: 0.936543
Ran for 55050 frames Cell Events: 3245 mean_circ: 0.936559
Ran for 56550 frames Cell Events: 3325 mean_circ: 0.936381
Ran for 57050 frames Cell Events: 3353 mean_circ: 0.936402
Ran for 58050 frames Cell Events: 3409 mean_circ: 0.936386
Ran for 58550 frames Cell Events: 3429 mean_circ: 0.93645
Ran for 59050 frames Cell Events: 3464 mean_circ: 0.936457
Ran for 60050 frames Cell Events: 3544 mean_circ: 0.936311
Ran for 61550 frames Cell Events: 3644 mean_circ: 0.936511
Ran for 63550 frames Cell Events: 3754 mean_circ: 0.936558
Ran for 64550 frames Cell Events: 3814 mean_circ: 0.936609
Ran for 66050 frames Cell Events: 3866 mean_circ: 0.9366
Ran for 67050 frames Cell Events: 3937 mean_circ: 0.936608
Ran for 67550 frames Cell Events: 3976 mean_circ: 0.936608
Ran for 68550 frames Cell Events: 4018 mean_circ: 0.936808
Ran for 70550 frames Cell Events: 4110 mean_circ: 0.936755
Ran for 72550 frames Cell Events: 4238 mean_circ: 0.936768
Ran for 73050 frames Cell Events: 4266 mean_circ: 0.936784
Ran for 73550 frames Cell Events: 4291 mean_circ: 0.936814
Ran for 74550 frames Cell Events: 4342 mean_circ: 0.93691
Ran for 76050 frames Cell Events: 4447 mean_circ: 0.936928
Ran for 76550 frames Cell Events: 4480 mean_circ: 0.936926
Ran for 77050 frames Cell Events: 4501 mean_circ: 0.936931
Ran for 77550 frames Cell Events: 4530 mean_circ: 0.936857
Ran for 78050 frames Cell Events: 4547 mean_circ: 0.936891
Ran for 78550 frames Cell Events: 4577 mean_circ: 0.936823
Ran for 79050 frames Cell Events: 4614 mean_circ: 0.936814
Ran for 80550 frames Cell Events: 4688 mean_circ: 0.936888
Ran for 82050 frames Cell Events: 4776 mean_circ: 0.9368
Ran for 83050 frames Cell Events: 4853 mean_circ: 0.936821
Ran for 83550 frames Cell Events: 4883 mean_circ: 0.936816
Ran for 85550 frames Cell Events: 4999 mean_circ: 0.936801
Ran for 87050 frames Cell Events: 5093 mean_circ: 0.936952
Ran for 87550 frames Cell Events: 5128 mean_circ: 0.936915
Ran for 88550 frames Cell Events: 5210 mean_circ: 0.936759
Ran for 95050 frames Cell Events: 5620 mean_circ: 0.936905
Ran for 96050 frames Cell Events: 5683 mean_circ: 0.936923
Ran for 96550 frames Cell Events: 5713 mean_circ: 0.936981
Ran for 97050 frames Cell Events: 5749 mean_circ: 0.936944
Ran for 98050 frames Cell Events: 5822 mean_circ: 0.936935
Ran for 98550 frames Cell Events: 5853 mean_circ: 0.936863
Ran for 99050 frames Cell Events: 5891 mean_circ: 0.936943
Ran for 100550 frames Cell Events: 5971 mean_circ: 0.936926
Ran for 101050 frames Cell Events: 6001 mean_circ: 0.936972
Ran for 104550 frames Cell Events: 6219 mean_circ: 0.937048
Ran for 105050 frames Cell Events: 6246 mean_circ: 0.937026
Ran for 105550 frames Cell Events: 6279 mean_circ: 0.937021
Ran for 106050 frames Cell Events: 6308 mean_circ: 0.937031
Ran for 106550 frames Cell Events: 6332 mean_circ: 0.937048
Ran for 107050 frames Cell Events: 6358 mean_circ: 0.937073
Ran for 107550 frames Cell Events: 6372 mean_circ: 0.937087
Ran for 109550 frames Cell Events: 6485 mean_circ: 0.937137
Ran for 110050 frames Cell Events: 6519 mean_circ: 0.937191
Ran for 110550 frames Cell Events: 6547 mean_circ: 0.937229
Ran for 112050 frames Cell Events: 6631 mean_circ: 0.937265
Ran for 112550 frames Cell Events: 6655 mean_circ: 0.937223
Ran for 115050 frames Cell Events: 6817 mean_circ: 0.937315
Ran for 115550 frames Cell Events: 6844 mean_circ: 0.93734
Ran for 116050 frames Cell Events: 6875 mean_circ: 0.937307
Ran for 117050 frames Cell Events: 6955 mean_circ: 0.937352
Ran for 118550 frames Cell Events: 7040 mean_circ: 0.937166
Ran for 119050 frames Cell Events: 7059 mean_circ: 0.937171
Ran for 120050 frames Cell Events: 7111 mean_circ: 0.937177
Ran for 120550 frames Cell Events: 7146 mean_circ: 0.937204
Ran for 122050 frames Cell Events: 7245 mean_circ: 0.937051
Ran for 122550 frames Cell Events: 7268 mean_circ: 0.937009
Ran for 123050 frames Cell Events: 7295 mean_circ: 0.936986
Ran for 123550 frames Cell Events: 7324 mean_circ: 0.936986
Ran for 124050 frames Cell Events: 7349 mean_circ: 0.936981
Ran for 124550 frames Cell Events: 7382 mean_circ: 0.937021
Ran for 125050 frames Cell Events: 7399 mean_circ: 0.936976
Ran for 125550 frames Cell Events: 7431 mean_circ: 0.936971
Ran for 127550 frames Cell Events: 7539 mean_circ: 0.937001
Ran for 128050 frames Cell Events: 7569 mean_circ: 0.937036
Ran for 129550 frames Cell Events: 7673 mean_circ: 0.936992
Ran for 132050 frames Cell Events: 7834 mean_circ: 0.937001
Ran for 132550 frames Cell Events: 7874 mean_circ: 0.937034
Ran for 133550 frames Cell Events: 7928 mean_circ: 0.937047
Ran for 135550 frames Cell Events: 8062 mean_circ: 0.936983
Ran for 136050 frames Cell Events: 8086 mean_circ: 0.936976



End this measurment..



Stopping time: 1.92698e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936973
Mean Area: 648.704
Mean FL-1 intensity: 3417.44
Mean FL-2 intensity: 4408.8
Mean FL-3 intensity: 0

frames processed: 135396
Total Time: 68702.8ms
Number of Runs (new bg img) 0
Frames collected: 137200
Skipped frames: 67128
Rescued Frames: 1671848
Samples DAQ: 34302344
Samples written: 68599744
Samples per Channel: 34299872
FPS: 1997.01

Samples per second: 499250
Expected samples/s: 500000

Events: 8161
Events per second: 118.787
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :214209
# 1 frames :48206
# 2 frames :2630
# 3 frames :945
# 4 frames :565
# 5 frames :202
# 6 frames :1710
# 7 frames :1088
# 8 frames :915
# 9 frames :542
# 10 frames :524
# 11 frames :493
# 12 frames :296
# 13 frames :194
# 14 frames :202
# 15 frames :203
# 16 frames :170
# 17 frames :146
# 18 frames :102
# 19 frames :83
# 20 frames :64
# 21 frames :69
# 22 frames :49
# 23 frames :52
# 24 frames :37
# 25 frames :41
# 26 frames :32
# 27 frames :25
# 28 frames :26
# 29 frames :21
# 30 frames :24
# 31 frames :19
# 32 frames :17
# 33 frames :11
# 34 frames :12
# 35 frames :8
# 36 frames :5
# 37 frames :6
# 38 frames :3
# 39 frames :6
# 40 frames :9
# 41 frames :4
# 42 frames :1
# 43 frames :3
# 44 frames :2
# 45 frames :3
# 46 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :3
# 54 frames :2
# 55 frames :2
# 56 frames :1
# 59 frames :1
# 62 frames :1
# 64 frames :1
# 72 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0443
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0