Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.46.17 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9f5aa22149fb53853fe91d0696ff56df",
"name": "M1_20170606_1549_dsRNA_O1-faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:46:37"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8180 (max 8181) in '8180'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0213
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92758e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 51 mean_circ: 0.946662
Ran for 1550 frames Cell Events: 77 mean_circ: 0.943618
Ran for 2050 frames Cell Events: 110 mean_circ: 0.944643
Ran for 3550 frames Cell Events: 203 mean_circ: 0.941953
Ran for 5050 frames Cell Events: 324 mean_circ: 0.939931
Ran for 7050 frames Cell Events: 487 mean_circ: 0.939848
Ran for 7550 frames Cell Events: 512 mean_circ: 0.940446
Ran for 9050 frames Cell Events: 619 mean_circ: 0.941549
Ran for 9550 frames Cell Events: 646 mean_circ: 0.941381
Ran for 11050 frames Cell Events: 710 mean_circ: 0.9413
Ran for 12050 frames Cell Events: 785 mean_circ: 0.94119
Ran for 12550 frames Cell Events: 822 mean_circ: 0.9413
Ran for 13050 frames Cell Events: 853 mean_circ: 0.940508
Ran for 16050 frames Cell Events: 1058 mean_circ: 0.940345
Ran for 17050 frames Cell Events: 1146 mean_circ: 0.940445
Ran for 18050 frames Cell Events: 1223 mean_circ: 0.94068
Ran for 18550 frames Cell Events: 1265 mean_circ: 0.940709
Ran for 19050 frames Cell Events: 1313 mean_circ: 0.940589
Ran for 20050 frames Cell Events: 1390 mean_circ: 0.940554
Ran for 21050 frames Cell Events: 1485 mean_circ: 0.940263
Ran for 23550 frames Cell Events: 1667 mean_circ: 0.940225
Ran for 25050 frames Cell Events: 1774 mean_circ: 0.94027
Ran for 27050 frames Cell Events: 1918 mean_circ: 0.940028
Ran for 27550 frames Cell Events: 1956 mean_circ: 0.939864
Ran for 28050 frames Cell Events: 1994 mean_circ: 0.939716
Ran for 35050 frames Cell Events: 2518 mean_circ: 0.939532
Ran for 35550 frames Cell Events: 2552 mean_circ: 0.939572
Ran for 36050 frames Cell Events: 2574 mean_circ: 0.939601
Ran for 37050 frames Cell Events: 2640 mean_circ: 0.939415
Ran for 38050 frames Cell Events: 2694 mean_circ: 0.939415
Ran for 38550 frames Cell Events: 2731 mean_circ: 0.939466
Ran for 39050 frames Cell Events: 2776 mean_circ: 0.939477
Ran for 40550 frames Cell Events: 2874 mean_circ: 0.939271
Ran for 41050 frames Cell Events: 2917 mean_circ: 0.939116
Ran for 42550 frames Cell Events: 3027 mean_circ: 0.939022
Ran for 43050 frames Cell Events: 3049 mean_circ: 0.93901
Ran for 44050 frames Cell Events: 3111 mean_circ: 0.939115
Ran for 46050 frames Cell Events: 3253 mean_circ: 0.939082
Ran for 47050 frames Cell Events: 3328 mean_circ: 0.938953
Ran for 48550 frames Cell Events: 3444 mean_circ: 0.938814
Ran for 49050 frames Cell Events: 3475 mean_circ: 0.938761
Ran for 49550 frames Cell Events: 3497 mean_circ: 0.938777
Ran for 50050 frames Cell Events: 3537 mean_circ: 0.93878
Ran for 51050 frames Cell Events: 3596 mean_circ: 0.93876
Ran for 52050 frames Cell Events: 3669 mean_circ: 0.938741
Ran for 54550 frames Cell Events: 3840 mean_circ: 0.938869
Ran for 56050 frames Cell Events: 3940 mean_circ: 0.938816
Ran for 56550 frames Cell Events: 3981 mean_circ: 0.938765
Ran for 58050 frames Cell Events: 4063 mean_circ: 0.938771
Ran for 59550 frames Cell Events: 4160 mean_circ: 0.938589
Ran for 61050 frames Cell Events: 4263 mean_circ: 0.938571
Ran for 61550 frames Cell Events: 4293 mean_circ: 0.938504
Ran for 62050 frames Cell Events: 4321 mean_circ: 0.938469
Ran for 62550 frames Cell Events: 4347 mean_circ: 0.938497
Ran for 64050 frames Cell Events: 4458 mean_circ: 0.938604
Ran for 64550 frames Cell Events: 4504 mean_circ: 0.938639
Ran for 67050 frames Cell Events: 4693 mean_circ: 0.938622
Ran for 67550 frames Cell Events: 4729 mean_circ: 0.938637
Ran for 68050 frames Cell Events: 4771 mean_circ: 0.938634
Ran for 68550 frames Cell Events: 4808 mean_circ: 0.938583
Ran for 69050 frames Cell Events: 4839 mean_circ: 0.938614
Ran for 69550 frames Cell Events: 4877 mean_circ: 0.938644
Ran for 70550 frames Cell Events: 4933 mean_circ: 0.938676
Ran for 71050 frames Cell Events: 4957 mean_circ: 0.938695
Ran for 71550 frames Cell Events: 4989 mean_circ: 0.938776
Ran for 72050 frames Cell Events: 5009 mean_circ: 0.938811
Ran for 72550 frames Cell Events: 5049 mean_circ: 0.938795
Ran for 74050 frames Cell Events: 5154 mean_circ: 0.938749
Ran for 74550 frames Cell Events: 5185 mean_circ: 0.938774
Ran for 76550 frames Cell Events: 5349 mean_circ: 0.938828
Ran for 77050 frames Cell Events: 5380 mean_circ: 0.938819
Ran for 77550 frames Cell Events: 5410 mean_circ: 0.938853
Ran for 82550 frames Cell Events: 5836 mean_circ: 0.938922
Ran for 83050 frames Cell Events: 5870 mean_circ: 0.93899
Ran for 85550 frames Cell Events: 6007 mean_circ: 0.938901
Ran for 86050 frames Cell Events: 6050 mean_circ: 0.938852
Ran for 88550 frames Cell Events: 6268 mean_circ: 0.93902
Ran for 91550 frames Cell Events: 6478 mean_circ: 0.939164
Ran for 92550 frames Cell Events: 6539 mean_circ: 0.939072
Ran for 93550 frames Cell Events: 6609 mean_circ: 0.939034
Ran for 94550 frames Cell Events: 6679 mean_circ: 0.939017
Ran for 95550 frames Cell Events: 6774 mean_circ: 0.938972
Ran for 96050 frames Cell Events: 6807 mean_circ: 0.938893
Ran for 99050 frames Cell Events: 7032 mean_circ: 0.938642
Ran for 99550 frames Cell Events: 7056 mean_circ: 0.938664
Ran for 100050 frames Cell Events: 7087 mean_circ: 0.938652
Ran for 102550 frames Cell Events: 7295 mean_circ: 0.938587
Ran for 103050 frames Cell Events: 7331 mean_circ: 0.938618
Ran for 105550 frames Cell Events: 7495 mean_circ: 0.938627
Ran for 107550 frames Cell Events: 7650 mean_circ: 0.938622
Ran for 109050 frames Cell Events: 7762 mean_circ: 0.938716
Ran for 109550 frames Cell Events: 7808 mean_circ: 0.938706
Ran for 112550 frames Cell Events: 8005 mean_circ: 0.938598



End this measurment..



Stopping time: 1.92764e+009
****************************************
Measurement finished

Mean Circularitiy: 0.938438
Mean Area: 636.05
Mean FL-1 intensity: 3829.01
Mean FL-2 intensity: 4984.69
Mean FL-3 intensity: 0

frames processed: 112189
Total Time: 57559.2ms
Number of Runs (new bg img) 0
Frames collected: 114961
Skipped frames: 72863
Rescued Frames: 1825778
Samples DAQ: 28740756
Samples written: 57480638
Samples per Channel: 28740319
FPS: 1997.27

Samples per second: 499318
Expected samples/s: 500000

Events: 8181
Events per second: 142.132
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :184870
# 1 frames :34531
# 2 frames :1883
# 3 frames :790
# 4 frames :355
# 5 frames :183
# 6 frames :1912
# 7 frames :824
# 8 frames :833
# 9 frames :479
# 10 frames :361
# 11 frames :253
# 12 frames :196
# 13 frames :170
# 14 frames :216
# 15 frames :207
# 16 frames :145
# 17 frames :118
# 18 frames :91
# 19 frames :79
# 20 frames :68
# 21 frames :77
# 22 frames :78
# 23 frames :48
# 24 frames :38
# 25 frames :30
# 26 frames :27
# 27 frames :26
# 28 frames :25
# 29 frames :26
# 30 frames :15
# 31 frames :24
# 32 frames :13
# 33 frames :9
# 34 frames :14
# 35 frames :7
# 36 frames :11
# 37 frames :9
# 38 frames :4
# 39 frames :11
# 40 frames :7
# 41 frames :5
# 42 frames :8
# 43 frames :3
# 44 frames :2
# 45 frames :3
# 46 frames :8
# 47 frames :4
# 48 frames :5
# 49 frames :1
# 50 frames :3
# 51 frames :6
# 52 frames :3
# 53 frames :1
# 55 frames :2
# 57 frames :1
# 58 frames :3
# 59 frames :2
# 60 frames :1
# 61 frames :2
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 68 frames :1
# 70 frames :1
# 71 frames :1
# 72 frames :1
# 76 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0213
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0