Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.55.09 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "55f8ace0922077e72746852636e644cd",
"name": "M1_20170606_1802_dsRNA_Z3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:55:32"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9395 (max 9396) in '9395'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2393
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.93558e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 12 mean_circ: 0.93209
Ran for 1550 frames Cell Events: 48 mean_circ: 0.94244
Ran for 2050 frames Cell Events: 72 mean_circ: 0.942886
Ran for 3050 frames Cell Events: 109 mean_circ: 0.945758
Ran for 3550 frames Cell Events: 125 mean_circ: 0.945729
Ran for 5050 frames Cell Events: 168 mean_circ: 0.946582
Ran for 5550 frames Cell Events: 181 mean_circ: 0.947376
Ran for 6550 frames Cell Events: 230 mean_circ: 0.946953
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Ran for 7550 frames Cell Events: 282 mean_circ: 0.948277
Ran for 8050 frames Cell Events: 295 mean_circ: 0.947332
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Ran for 9550 frames Cell Events: 344 mean_circ: 0.94591
Ran for 10050 frames Cell Events: 368 mean_circ: 0.945926
Ran for 10550 frames Cell Events: 389 mean_circ: 0.946335
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Ran for 11550 frames Cell Events: 431 mean_circ: 0.947053
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Ran for 13550 frames Cell Events: 502 mean_circ: 0.947266
Ran for 14050 frames Cell Events: 526 mean_circ: 0.947026
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Ran for 20050 frames Cell Events: 748 mean_circ: 0.947005
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Ran for 67050 frames Cell Events: 2415 mean_circ: 0.947335
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Ran for 73050 frames Cell Events: 2629 mean_circ: 0.947134
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Ran for 74050 frames Cell Events: 2654 mean_circ: 0.9472
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Ran for 79550 frames Cell Events: 2854 mean_circ: 0.946921
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Ran for 81050 frames Cell Events: 2911 mean_circ: 0.947109
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Ran for 82050 frames Cell Events: 2951 mean_circ: 0.946921
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Ran for 83050 frames Cell Events: 2979 mean_circ: 0.946894
Ran for 83550 frames Cell Events: 2996 mean_circ: 0.946932
Ran for 84050 frames Cell Events: 3011 mean_circ: 0.946959
Ran for 84550 frames Cell Events: 3029 mean_circ: 0.947001
Ran for 85550 frames Cell Events: 3062 mean_circ: 0.946952
Ran for 86050 frames Cell Events: 3088 mean_circ: 0.946966
Ran for 86550 frames Cell Events: 3104 mean_circ: 0.946909
Ran for 87050 frames Cell Events: 3118 mean_circ: 0.946944
Ran for 87550 frames Cell Events: 3141 mean_circ: 0.94691
Ran for 88050 frames Cell Events: 3157 mean_circ: 0.946915
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Ran for 90050 frames Cell Events: 3225 mean_circ: 0.946785
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Ran for 91050 frames Cell Events: 3268 mean_circ: 0.94686
Ran for 93050 frames Cell Events: 3334 mean_circ: 0.946819
Ran for 93550 frames Cell Events: 3353 mean_circ: 0.946851
Ran for 94050 frames Cell Events: 3381 mean_circ: 0.946876
Ran for 95050 frames Cell Events: 3424 mean_circ: 0.946824
Ran for 95550 frames Cell Events: 3450 mean_circ: 0.946743
Ran for 96050 frames Cell Events: 3468 mean_circ: 0.94674
Ran for 97550 frames Cell Events: 3511 mean_circ: 0.946584
Ran for 98550 frames Cell Events: 3552 mean_circ: 0.946618
Ran for 99550 frames Cell Events: 3599 mean_circ: 0.946663
Ran for 100050 frames Cell Events: 3611 mean_circ: 0.946686
Ran for 100550 frames Cell Events: 3633 mean_circ: 0.946601
Ran for 103050 frames Cell Events: 3734 mean_circ: 0.94631
Ran for 103550 frames Cell Events: 3753 mean_circ: 0.946316
Ran for 104550 frames Cell Events: 3784 mean_circ: 0.946406
Ran for 106050 frames Cell Events: 3836 mean_circ: 0.946462
Ran for 107050 frames Cell Events: 3865 mean_circ: 0.946451
Ran for 107550 frames Cell Events: 3885 mean_circ: 0.946476
Ran for 108050 frames Cell Events: 3907 mean_circ: 0.946538
Ran for 108550 frames Cell Events: 3924 mean_circ: 0.946569
Ran for 111550 frames Cell Events: 4037 mean_circ: 0.946536
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Ran for 114050 frames Cell Events: 4119 mean_circ: 0.946428
Ran for 115550 frames Cell Events: 4180 mean_circ: 0.946315
Ran for 117050 frames Cell Events: 4230 mean_circ: 0.946207
Ran for 118050 frames Cell Events: 4260 mean_circ: 0.946121
Ran for 119050 frames Cell Events: 4287 mean_circ: 0.946099
Ran for 121050 frames Cell Events: 4383 mean_circ: 0.945984
Ran for 121550 frames Cell Events: 4398 mean_circ: 0.945935
Ran for 122050 frames Cell Events: 4416 mean_circ: 0.94591
Ran for 122550 frames Cell Events: 4440 mean_circ: 0.945876
Ran for 123050 frames Cell Events: 4458 mean_circ: 0.945862
Ran for 126550 frames Cell Events: 4590 mean_circ: 0.945603
Ran for 127550 frames Cell Events: 4630 mean_circ: 0.945593
Ran for 129550 frames Cell Events: 4705 mean_circ: 0.94572
Ran for 130050 frames Cell Events: 4730 mean_circ: 0.945781
Ran for 132050 frames Cell Events: 4817 mean_circ: 0.945808
Ran for 132550 frames Cell Events: 4844 mean_circ: 0.94573
Ran for 136050 frames Cell Events: 4989 mean_circ: 0.945733
Ran for 136550 frames Cell Events: 5008 mean_circ: 0.945793
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Ran for 141550 frames Cell Events: 5202 mean_circ: 0.945814
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Ran for 143050 frames Cell Events: 5258 mean_circ: 0.945863
Ran for 144050 frames Cell Events: 5294 mean_circ: 0.945875
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Ran for 146050 frames Cell Events: 5368 mean_circ: 0.945881
Ran for 146550 frames Cell Events: 5392 mean_circ: 0.945929
Ran for 147050 frames Cell Events: 5409 mean_circ: 0.945964
Ran for 147550 frames Cell Events: 5428 mean_circ: 0.94596
Ran for 148050 frames Cell Events: 5445 mean_circ: 0.945923
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Ran for 152050 frames Cell Events: 5578 mean_circ: 0.945817
Ran for 152550 frames Cell Events: 5606 mean_circ: 0.945817
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Ran for 153550 frames Cell Events: 5656 mean_circ: 0.945778
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Ran for 202050 frames Cell Events: 7543 mean_circ: 0.945218
Ran for 202550 frames Cell Events: 7565 mean_circ: 0.945195
Ran for 203050 frames Cell Events: 7584 mean_circ: 0.945177
Ran for 204050 frames Cell Events: 7623 mean_circ: 0.945143
Ran for 205550 frames Cell Events: 7695 mean_circ: 0.945187
Ran for 206050 frames Cell Events: 7710 mean_circ: 0.945177
Ran for 207550 frames Cell Events: 7772 mean_circ: 0.945216
Ran for 208050 frames Cell Events: 7792 mean_circ: 0.945191
Ran for 208550 frames Cell Events: 7817 mean_circ: 0.9452
Ran for 209050 frames Cell Events: 7835 mean_circ: 0.945213
Ran for 209550 frames Cell Events: 7854 mean_circ: 0.945197
Ran for 210050 frames Cell Events: 7875 mean_circ: 0.945163
Ran for 211050 frames Cell Events: 7915 mean_circ: 0.945191
Ran for 211550 frames Cell Events: 7935 mean_circ: 0.945142
Ran for 212550 frames Cell Events: 7982 mean_circ: 0.945117
Ran for 213050 frames Cell Events: 8002 mean_circ: 0.945089
Ran for 213550 frames Cell Events: 8035 mean_circ: 0.94511
Ran for 214050 frames Cell Events: 8044 mean_circ: 0.945126
Ran for 214550 frames Cell Events: 8063 mean_circ: 0.945157
Ran for 215550 frames Cell Events: 8109 mean_circ: 0.945174
Ran for 217050 frames Cell Events: 8186 mean_circ: 0.945259
Ran for 217550 frames Cell Events: 8207 mean_circ: 0.945232
Ran for 218050 frames Cell Events: 8234 mean_circ: 0.945217
Ran for 219050 frames Cell Events: 8278 mean_circ: 0.945234
Ran for 219550 frames Cell Events: 8294 mean_circ: 0.94529
Ran for 220050 frames Cell Events: 8327 mean_circ: 0.945242
Ran for 220550 frames Cell Events: 8351 mean_circ: 0.945242
Ran for 221050 frames Cell Events: 8376 mean_circ: 0.945265
Ran for 221550 frames Cell Events: 8397 mean_circ: 0.945257
Ran for 223550 frames Cell Events: 8477 mean_circ: 0.945289
Ran for 224050 frames Cell Events: 8497 mean_circ: 0.945284
Ran for 225050 frames Cell Events: 8525 mean_circ: 0.945301
Ran for 225550 frames Cell Events: 8544 mean_circ: 0.945267
Ran for 226550 frames Cell Events: 8582 mean_circ: 0.94528
Ran for 227050 frames Cell Events: 8609 mean_circ: 0.945318
Ran for 227550 frames Cell Events: 8631 mean_circ: 0.945291
Ran for 228050 frames Cell Events: 8650 mean_circ: 0.945249
Ran for 228550 frames Cell Events: 8670 mean_circ: 0.945246
Ran for 229050 frames Cell Events: 8695 mean_circ: 0.945267
Ran for 230550 frames Cell Events: 8746 mean_circ: 0.945269
Ran for 231050 frames Cell Events: 8756 mean_circ: 0.945249
Ran for 231550 frames Cell Events: 8769 mean_circ: 0.945238
Ran for 232050 frames Cell Events: 8784 mean_circ: 0.945245
Ran for 232550 frames Cell Events: 8798 mean_circ: 0.945225
Ran for 233050 frames Cell Events: 8819 mean_circ: 0.9452
Ran for 233550 frames Cell Events: 8836 mean_circ: 0.945245
Ran for 234550 frames Cell Events: 8862 mean_circ: 0.945295
Ran for 235050 frames Cell Events: 8885 mean_circ: 0.945268
Ran for 235550 frames Cell Events: 8903 mean_circ: 0.945274
Ran for 236050 frames Cell Events: 8923 mean_circ: 0.945258
Ran for 236550 frames Cell Events: 8947 mean_circ: 0.945241
Ran for 237050 frames Cell Events: 8970 mean_circ: 0.945238
Ran for 238050 frames Cell Events: 9006 mean_circ: 0.945246
Ran for 238550 frames Cell Events: 9025 mean_circ: 0.945241
Ran for 239050 frames Cell Events: 9044 mean_circ: 0.945236
Ran for 239550 frames Cell Events: 9059 mean_circ: 0.945193
Ran for 240050 frames Cell Events: 9075 mean_circ: 0.945208
Ran for 240550 frames Cell Events: 9097 mean_circ: 0.945239
Ran for 241050 frames Cell Events: 9121 mean_circ: 0.945242
Ran for 241550 frames Cell Events: 9143 mean_circ: 0.945256
Ran for 242050 frames Cell Events: 9157 mean_circ: 0.945245
Ran for 242550 frames Cell Events: 9178 mean_circ: 0.945237
Ran for 243050 frames Cell Events: 9199 mean_circ: 0.945242
Ran for 243550 frames Cell Events: 9218 mean_circ: 0.945248
Ran for 244550 frames Cell Events: 9235 mean_circ: 0.94523
Ran for 245050 frames Cell Events: 9255 mean_circ: 0.945237
Ran for 245550 frames Cell Events: 9276 mean_circ: 0.945266
Ran for 246550 frames Cell Events: 9321 mean_circ: 0.945301
Ran for 247050 frames Cell Events: 9342 mean_circ: 0.945283
Ran for 247550 frames Cell Events: 9366 mean_circ: 0.945283
Ran for 248050 frames Cell Events: 9381 mean_circ: 0.945266



End this measurment..



Stopping time: 1.9357e+009
****************************************
Measurement finished

Mean Circularitiy: 0.945294
Mean Area: 646.997
Mean FL-1 intensity: 3970.17
Mean FL-2 intensity: 3968.62
Mean FL-3 intensity: 0

frames processed: 246315
Total Time: 124268ms
Number of Runs (new bg img) 0
Frames collected: 248372
Skipped frames: 103224
Rescued Frames: 1780558
Samples DAQ: 62092981
Samples written: 124185780
Samples per Channel: 62092890
FPS: 1998.68

Samples per second: 499669
Expected samples/s: 500000

Events: 9396
Events per second: 75.6107
jumped back (PMT): 6117
#Frames read, frequency
# 0 frames :353699
# 1 frames :124126
# 2 frames :4894
# 3 frames :2003
# 4 frames :925
# 5 frames :2390
# 6 frames :2041
# 7 frames :1467
# 8 frames :918
# 9 frames :679
# 10 frames :636
# 11 frames :499
# 12 frames :367
# 13 frames :343
# 14 frames :277
# 15 frames :221
# 16 frames :173
# 17 frames :152
# 18 frames :115
# 19 frames :125
# 20 frames :93
# 21 frames :77
# 22 frames :63
# 23 frames :63
# 24 frames :50
# 25 frames :31
# 26 frames :40
# 27 frames :34
# 28 frames :28
# 29 frames :26
# 30 frames :21
# 31 frames :19
# 32 frames :18
# 33 frames :12
# 34 frames :10
# 35 frames :10
# 36 frames :10
# 37 frames :11
# 38 frames :5
# 39 frames :5
# 40 frames :4
# 41 frames :6
# 42 frames :6
# 43 frames :2
# 44 frames :1
# 45 frames :3
# 46 frames :2
# 47 frames :2
# 48 frames :2
# 49 frames :1
# 51 frames :2
# 52 frames :1
# 54 frames :1
# 57 frames :1
# 59 frames :1
# 69 frames :1
# 73 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2393
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0