Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.03.24 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "69c40e77b1609bfde3679ee32314dad9",
"name": "M1_20170606_2010_dsRNA_P6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:03:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9064 (max 9066) in '9064'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1521
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.94326e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 51 mean_circ: 0.913285
Ran for 1550 frames Cell Events: 82 mean_circ: 0.931437
Ran for 5050 frames Cell Events: 329 mean_circ: 0.946578
Ran for 6050 frames Cell Events: 406 mean_circ: 0.946574
Ran for 6550 frames Cell Events: 429 mean_circ: 0.947103
Ran for 8550 frames Cell Events: 537 mean_circ: 0.948592
Ran for 9050 frames Cell Events: 562 mean_circ: 0.948923
Ran for 10050 frames Cell Events: 624 mean_circ: 0.948872
Ran for 10550 frames Cell Events: 656 mean_circ: 0.949292
Ran for 11050 frames Cell Events: 690 mean_circ: 0.949292
Ran for 11550 frames Cell Events: 737 mean_circ: 0.949915
Ran for 13050 frames Cell Events: 830 mean_circ: 0.950199
Ran for 13550 frames Cell Events: 851 mean_circ: 0.950279
Ran for 14550 frames Cell Events: 932 mean_circ: 0.950659
Ran for 15550 frames Cell Events: 986 mean_circ: 0.950467
Ran for 16050 frames Cell Events: 1021 mean_circ: 0.95081
Ran for 18050 frames Cell Events: 1147 mean_circ: 0.951146
Ran for 18550 frames Cell Events: 1184 mean_circ: 0.951141
Ran for 19550 frames Cell Events: 1225 mean_circ: 0.95135
Ran for 20550 frames Cell Events: 1279 mean_circ: 0.951114
Ran for 21550 frames Cell Events: 1339 mean_circ: 0.951144
Ran for 22050 frames Cell Events: 1368 mean_circ: 0.951271
Ran for 22550 frames Cell Events: 1410 mean_circ: 0.951178
Ran for 23050 frames Cell Events: 1448 mean_circ: 0.951206
Ran for 23550 frames Cell Events: 1476 mean_circ: 0.951324
Ran for 24050 frames Cell Events: 1512 mean_circ: 0.951414
Ran for 26050 frames Cell Events: 1612 mean_circ: 0.951637
Ran for 27050 frames Cell Events: 1681 mean_circ: 0.951488
Ran for 27550 frames Cell Events: 1701 mean_circ: 0.951387
Ran for 28550 frames Cell Events: 1777 mean_circ: 0.951452
Ran for 29050 frames Cell Events: 1800 mean_circ: 0.951433
Ran for 29550 frames Cell Events: 1824 mean_circ: 0.951406
Ran for 30550 frames Cell Events: 1889 mean_circ: 0.951566
Ran for 31550 frames Cell Events: 1940 mean_circ: 0.951802
Ran for 32050 frames Cell Events: 1965 mean_circ: 0.951779
Ran for 33550 frames Cell Events: 2043 mean_circ: 0.951547
Ran for 34550 frames Cell Events: 2086 mean_circ: 0.951489
Ran for 36550 frames Cell Events: 2194 mean_circ: 0.951636
Ran for 37550 frames Cell Events: 2266 mean_circ: 0.951812
Ran for 38050 frames Cell Events: 2306 mean_circ: 0.95172
Ran for 38550 frames Cell Events: 2338 mean_circ: 0.951773
Ran for 39050 frames Cell Events: 2369 mean_circ: 0.951878
Ran for 39550 frames Cell Events: 2395 mean_circ: 0.951823
Ran for 40050 frames Cell Events: 2419 mean_circ: 0.951873
Ran for 41050 frames Cell Events: 2475 mean_circ: 0.951858
Ran for 42050 frames Cell Events: 2545 mean_circ: 0.951706
Ran for 43550 frames Cell Events: 2628 mean_circ: 0.951789
Ran for 44050 frames Cell Events: 2662 mean_circ: 0.951889
Ran for 45050 frames Cell Events: 2707 mean_circ: 0.951893
Ran for 45550 frames Cell Events: 2747 mean_circ: 0.951871
Ran for 46050 frames Cell Events: 2787 mean_circ: 0.951862
Ran for 48050 frames Cell Events: 2935 mean_circ: 0.951713
Ran for 48550 frames Cell Events: 2958 mean_circ: 0.951729
Ran for 51050 frames Cell Events: 3115 mean_circ: 0.951775
Ran for 51550 frames Cell Events: 3139 mean_circ: 0.951762
Ran for 52550 frames Cell Events: 3199 mean_circ: 0.951936
Ran for 53550 frames Cell Events: 3241 mean_circ: 0.951929
Ran for 54550 frames Cell Events: 3300 mean_circ: 0.951789
Ran for 56050 frames Cell Events: 3399 mean_circ: 0.951539
Ran for 56550 frames Cell Events: 3429 mean_circ: 0.951601
Ran for 57050 frames Cell Events: 3456 mean_circ: 0.951552
Ran for 58550 frames Cell Events: 3545 mean_circ: 0.951574
Ran for 61050 frames Cell Events: 3683 mean_circ: 0.951656
Ran for 61550 frames Cell Events: 3708 mean_circ: 0.951628
Ran for 62050 frames Cell Events: 3748 mean_circ: 0.95172
Ran for 62550 frames Cell Events: 3766 mean_circ: 0.951811
Ran for 63050 frames Cell Events: 3792 mean_circ: 0.951846
Ran for 64050 frames Cell Events: 3848 mean_circ: 0.951819
Ran for 64550 frames Cell Events: 3862 mean_circ: 0.95185
Ran for 65050 frames Cell Events: 3888 mean_circ: 0.951843
Ran for 66050 frames Cell Events: 3949 mean_circ: 0.951836
Ran for 66550 frames Cell Events: 3972 mean_circ: 0.951866
Ran for 68550 frames Cell Events: 4093 mean_circ: 0.951768
Ran for 69550 frames Cell Events: 4169 mean_circ: 0.951668
Ran for 70550 frames Cell Events: 4238 mean_circ: 0.951722
Ran for 71550 frames Cell Events: 4310 mean_circ: 0.95142
Ran for 72050 frames Cell Events: 4344 mean_circ: 0.951378
Ran for 72550 frames Cell Events: 4365 mean_circ: 0.951382
Ran for 73050 frames Cell Events: 4398 mean_circ: 0.951295
Ran for 74050 frames Cell Events: 4456 mean_circ: 0.951111
Ran for 74550 frames Cell Events: 4479 mean_circ: 0.9511
Ran for 75050 frames Cell Events: 4511 mean_circ: 0.951114
Ran for 75550 frames Cell Events: 4539 mean_circ: 0.951074
Ran for 76050 frames Cell Events: 4576 mean_circ: 0.951121
Ran for 76550 frames Cell Events: 4599 mean_circ: 0.951215
Ran for 77050 frames Cell Events: 4635 mean_circ: 0.951215
Ran for 79050 frames Cell Events: 4757 mean_circ: 0.951352
Ran for 79550 frames Cell Events: 4786 mean_circ: 0.951348
Ran for 80050 frames Cell Events: 4820 mean_circ: 0.95127
Ran for 80550 frames Cell Events: 4846 mean_circ: 0.951318
Ran for 81050 frames Cell Events: 4866 mean_circ: 0.951334
Ran for 81550 frames Cell Events: 4900 mean_circ: 0.951328
Ran for 82550 frames Cell Events: 4975 mean_circ: 0.951296
Ran for 83550 frames Cell Events: 5015 mean_circ: 0.951299
Ran for 84050 frames Cell Events: 5045 mean_circ: 0.951293
Ran for 85550 frames Cell Events: 5185 mean_circ: 0.951064
Ran for 86050 frames Cell Events: 5216 mean_circ: 0.951035
Ran for 86550 frames Cell Events: 5241 mean_circ: 0.950974
Ran for 87550 frames Cell Events: 5303 mean_circ: 0.951008
Ran for 88050 frames Cell Events: 5336 mean_circ: 0.951039
Ran for 89050 frames Cell Events: 5384 mean_circ: 0.951081
Ran for 89550 frames Cell Events: 5427 mean_circ: 0.951046
Ran for 91050 frames Cell Events: 5523 mean_circ: 0.951024
Ran for 92550 frames Cell Events: 5619 mean_circ: 0.950994
Ran for 93550 frames Cell Events: 5671 mean_circ: 0.951019
Ran for 94050 frames Cell Events: 5695 mean_circ: 0.951046
Ran for 94550 frames Cell Events: 5740 mean_circ: 0.95104
Ran for 95050 frames Cell Events: 5771 mean_circ: 0.951064
Ran for 95550 frames Cell Events: 5808 mean_circ: 0.95115
Ran for 96050 frames Cell Events: 5831 mean_circ: 0.951118
Ran for 96550 frames Cell Events: 5856 mean_circ: 0.951135
Ran for 97050 frames Cell Events: 5872 mean_circ: 0.951133
Ran for 97550 frames Cell Events: 5897 mean_circ: 0.951154
Ran for 99550 frames Cell Events: 6012 mean_circ: 0.950934
Ran for 100050 frames Cell Events: 6031 mean_circ: 0.950934
Ran for 100550 frames Cell Events: 6064 mean_circ: 0.950837
Ran for 101050 frames Cell Events: 6089 mean_circ: 0.950811
Ran for 102550 frames Cell Events: 6170 mean_circ: 0.950845
Ran for 103050 frames Cell Events: 6194 mean_circ: 0.950834
Ran for 103550 frames Cell Events: 6223 mean_circ: 0.950845
Ran for 104050 frames Cell Events: 6248 mean_circ: 0.950874
Ran for 105550 frames Cell Events: 6336 mean_circ: 0.950867
Ran for 107550 frames Cell Events: 6464 mean_circ: 0.950804
Ran for 108550 frames Cell Events: 6532 mean_circ: 0.950665
Ran for 109050 frames Cell Events: 6565 mean_circ: 0.950607
Ran for 110050 frames Cell Events: 6633 mean_circ: 0.950558
Ran for 110550 frames Cell Events: 6668 mean_circ: 0.950563
Ran for 111550 frames Cell Events: 6715 mean_circ: 0.950448
Ran for 112050 frames Cell Events: 6745 mean_circ: 0.950445
Ran for 113050 frames Cell Events: 6827 mean_circ: 0.950334
Ran for 114050 frames Cell Events: 6898 mean_circ: 0.950303
Ran for 116050 frames Cell Events: 7038 mean_circ: 0.950252
Ran for 117050 frames Cell Events: 7112 mean_circ: 0.95028
Ran for 118050 frames Cell Events: 7179 mean_circ: 0.950348
Ran for 118550 frames Cell Events: 7196 mean_circ: 0.950355
Ran for 119550 frames Cell Events: 7266 mean_circ: 0.950438
Ran for 120550 frames Cell Events: 7352 mean_circ: 0.950294
Ran for 121550 frames Cell Events: 7421 mean_circ: 0.950306
Ran for 122050 frames Cell Events: 7448 mean_circ: 0.950317
Ran for 122550 frames Cell Events: 7470 mean_circ: 0.950345
Ran for 123050 frames Cell Events: 7502 mean_circ: 0.950317
Ran for 124050 frames Cell Events: 7554 mean_circ: 0.950333
Ran for 124550 frames Cell Events: 7585 mean_circ: 0.950329
Ran for 126050 frames Cell Events: 7743 mean_circ: 0.950355
Ran for 127550 frames Cell Events: 7875 mean_circ: 0.950296
Ran for 128050 frames Cell Events: 7898 mean_circ: 0.950306
Ran for 129050 frames Cell Events: 7956 mean_circ: 0.950341
Ran for 130050 frames Cell Events: 8002 mean_circ: 0.950351
Ran for 130550 frames Cell Events: 8031 mean_circ: 0.950356
Ran for 131550 frames Cell Events: 8081 mean_circ: 0.950373
Ran for 132050 frames Cell Events: 8100 mean_circ: 0.950412
Ran for 134050 frames Cell Events: 8218 mean_circ: 0.950451
Ran for 135550 frames Cell Events: 8301 mean_circ: 0.950429
Ran for 137550 frames Cell Events: 8429 mean_circ: 0.950353
Ran for 138050 frames Cell Events: 8464 mean_circ: 0.950366
Ran for 139050 frames Cell Events: 8526 mean_circ: 0.950313
Ran for 140550 frames Cell Events: 8615 mean_circ: 0.950282
Ran for 141550 frames Cell Events: 8684 mean_circ: 0.950267
Ran for 143550 frames Cell Events: 8782 mean_circ: 0.950249
Ran for 144050 frames Cell Events: 8820 mean_circ: 0.950265
Ran for 145050 frames Cell Events: 8889 mean_circ: 0.950136
Ran for 145550 frames Cell Events: 8922 mean_circ: 0.950115
Ran for 146050 frames Cell Events: 8954 mean_circ: 0.950061
Ran for 146550 frames Cell Events: 8991 mean_circ: 0.950067
Ran for 147550 frames Cell Events: 9065 mean_circ: 0.950046



End this measurment..



Stopping time: 1.94333e+009
****************************************
Measurement finished

Mean Circularitiy: 0.950034
Mean Area: 628.676
Mean FL-1 intensity: 4317.09
Mean FL-2 intensity: 4596.08
Mean FL-3 intensity: 0

frames processed: 147365
Total Time: 73884.5ms
Number of Runs (new bg img) 0
Frames collected: 147592
Skipped frames: 141395
Rescued Frames: 3566260
Samples DAQ: 36898134
Samples written: 73796174
Samples per Channel: 36898087
FPS: 1997.61

Samples per second: 499402
Expected samples/s: 500000

Events: 9066
Events per second: 122.705
jumped back (PMT): 4029
#Frames read, frequency
# 0 frames :223682
# 1 frames :57944
# 2 frames :3273
# 3 frames :1268
# 4 frames :515
# 5 frames :2049
# 6 frames :1390
# 7 frames :1114
# 8 frames :572
# 9 frames :382
# 10 frames :647
# 11 frames :363
# 12 frames :345
# 13 frames :249
# 14 frames :222
# 15 frames :191
# 16 frames :148
# 17 frames :151
# 18 frames :107
# 19 frames :88
# 20 frames :68
# 21 frames :52
# 22 frames :47
# 23 frames :32
# 24 frames :33
# 25 frames :28
# 26 frames :24
# 27 frames :17
# 28 frames :15
# 29 frames :13
# 30 frames :12
# 31 frames :3
# 32 frames :10
# 33 frames :6
# 34 frames :5
# 35 frames :2
# 36 frames :1
# 37 frames :4
# 38 frames :8
# 39 frames :3
# 40 frames :2
# 41 frames :6
# 42 frames :4
# 43 frames :2
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 48 frames :2
# 52 frames :2
# 61 frames :1
# 62 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1521
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0