Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.11.55 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0b5d974b48b2e0704a4238ead4b29478",
"name": "M1_20170606_2156_dsRNA_Q6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:12:14"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8051 (max 8052) in '8051'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1732
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.9496e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 71 mean_circ: 0.94676
Ran for 3050 frames Cell Events: 153 mean_circ: 0.947899
Ran for 5050 frames Cell Events: 246 mean_circ: 0.94845
Ran for 5550 frames Cell Events: 272 mean_circ: 0.948746
Ran for 6050 frames Cell Events: 280 mean_circ: 0.948476
Ran for 7550 frames Cell Events: 364 mean_circ: 0.949519
Ran for 9050 frames Cell Events: 433 mean_circ: 0.949528
Ran for 10050 frames Cell Events: 482 mean_circ: 0.950063
Ran for 10550 frames Cell Events: 510 mean_circ: 0.950525
Ran for 11050 frames Cell Events: 539 mean_circ: 0.950267
Ran for 12050 frames Cell Events: 619 mean_circ: 0.950485
Ran for 13050 frames Cell Events: 672 mean_circ: 0.950946
Ran for 14050 frames Cell Events: 707 mean_circ: 0.950899
Ran for 15050 frames Cell Events: 739 mean_circ: 0.950621
Ran for 15550 frames Cell Events: 762 mean_circ: 0.950775
Ran for 16050 frames Cell Events: 777 mean_circ: 0.95105
Ran for 17050 frames Cell Events: 832 mean_circ: 0.950945
Ran for 17550 frames Cell Events: 853 mean_circ: 0.950479
Ran for 18050 frames Cell Events: 883 mean_circ: 0.950643
Ran for 20050 frames Cell Events: 987 mean_circ: 0.951212
Ran for 21050 frames Cell Events: 1045 mean_circ: 0.951287
Ran for 21550 frames Cell Events: 1056 mean_circ: 0.951463
Ran for 22050 frames Cell Events: 1073 mean_circ: 0.951347
Ran for 23050 frames Cell Events: 1105 mean_circ: 0.951531
Ran for 24050 frames Cell Events: 1163 mean_circ: 0.951801
Ran for 24550 frames Cell Events: 1180 mean_circ: 0.951954
Ran for 25050 frames Cell Events: 1193 mean_circ: 0.952042
Ran for 26550 frames Cell Events: 1289 mean_circ: 0.95181
Ran for 27550 frames Cell Events: 1365 mean_circ: 0.951803
Ran for 28050 frames Cell Events: 1390 mean_circ: 0.951687
Ran for 28550 frames Cell Events: 1406 mean_circ: 0.951378
Ran for 29050 frames Cell Events: 1439 mean_circ: 0.951108
Ran for 31550 frames Cell Events: 1556 mean_circ: 0.951006
Ran for 32550 frames Cell Events: 1641 mean_circ: 0.950982
Ran for 33050 frames Cell Events: 1657 mean_circ: 0.951013
Ran for 34550 frames Cell Events: 1754 mean_circ: 0.951253
Ran for 35550 frames Cell Events: 1811 mean_circ: 0.951316
Ran for 38050 frames Cell Events: 1975 mean_circ: 0.950831
Ran for 39050 frames Cell Events: 2055 mean_circ: 0.951065
Ran for 39550 frames Cell Events: 2093 mean_circ: 0.950679
Ran for 40550 frames Cell Events: 2144 mean_circ: 0.95088
Ran for 42550 frames Cell Events: 2278 mean_circ: 0.950881
Ran for 44050 frames Cell Events: 2358 mean_circ: 0.950793
Ran for 45050 frames Cell Events: 2427 mean_circ: 0.950909
Ran for 45550 frames Cell Events: 2455 mean_circ: 0.950963
Ran for 46050 frames Cell Events: 2493 mean_circ: 0.950988
Ran for 46550 frames Cell Events: 2522 mean_circ: 0.950914
Ran for 48050 frames Cell Events: 2582 mean_circ: 0.951048
Ran for 48550 frames Cell Events: 2597 mean_circ: 0.950982
Ran for 49550 frames Cell Events: 2639 mean_circ: 0.950888
Ran for 50550 frames Cell Events: 2697 mean_circ: 0.950878
Ran for 51550 frames Cell Events: 2746 mean_circ: 0.950997
Ran for 52050 frames Cell Events: 2790 mean_circ: 0.950979
Ran for 52550 frames Cell Events: 2818 mean_circ: 0.951023
Ran for 53550 frames Cell Events: 2890 mean_circ: 0.950967
Ran for 54050 frames Cell Events: 2921 mean_circ: 0.950855
Ran for 55050 frames Cell Events: 2986 mean_circ: 0.950859
Ran for 56050 frames Cell Events: 3028 mean_circ: 0.950875
Ran for 56550 frames Cell Events: 3047 mean_circ: 0.950922
Ran for 57550 frames Cell Events: 3097 mean_circ: 0.951071
Ran for 58050 frames Cell Events: 3119 mean_circ: 0.951127
Ran for 59550 frames Cell Events: 3190 mean_circ: 0.951209
Ran for 61050 frames Cell Events: 3285 mean_circ: 0.95125
Ran for 61550 frames Cell Events: 3303 mean_circ: 0.951315
Ran for 62550 frames Cell Events: 3353 mean_circ: 0.951272
Ran for 64050 frames Cell Events: 3434 mean_circ: 0.951118
Ran for 64550 frames Cell Events: 3449 mean_circ: 0.951146
Ran for 66050 frames Cell Events: 3520 mean_circ: 0.951198
Ran for 67050 frames Cell Events: 3584 mean_circ: 0.951165
Ran for 67550 frames Cell Events: 3602 mean_circ: 0.951041
Ran for 68550 frames Cell Events: 3662 mean_circ: 0.950984
Ran for 69050 frames Cell Events: 3692 mean_circ: 0.951038
Ran for 69550 frames Cell Events: 3716 mean_circ: 0.951045
Ran for 70550 frames Cell Events: 3772 mean_circ: 0.951053
Ran for 71050 frames Cell Events: 3795 mean_circ: 0.951106
Ran for 72050 frames Cell Events: 3841 mean_circ: 0.951144
Ran for 72550 frames Cell Events: 3858 mean_circ: 0.951145
Ran for 73050 frames Cell Events: 3899 mean_circ: 0.951025
Ran for 73550 frames Cell Events: 3924 mean_circ: 0.951007
Ran for 74550 frames Cell Events: 3982 mean_circ: 0.951006
Ran for 75050 frames Cell Events: 4006 mean_circ: 0.95105
Ran for 75550 frames Cell Events: 4035 mean_circ: 0.951031
Ran for 77050 frames Cell Events: 4119 mean_circ: 0.950999
Ran for 77550 frames Cell Events: 4157 mean_circ: 0.950972
Ran for 78050 frames Cell Events: 4188 mean_circ: 0.950951
Ran for 78550 frames Cell Events: 4222 mean_circ: 0.950869
Ran for 79050 frames Cell Events: 4257 mean_circ: 0.950791
Ran for 80050 frames Cell Events: 4326 mean_circ: 0.950848
Ran for 80550 frames Cell Events: 4348 mean_circ: 0.950866
Ran for 81050 frames Cell Events: 4358 mean_circ: 0.950937
Ran for 81550 frames Cell Events: 4387 mean_circ: 0.950952
Ran for 82050 frames Cell Events: 4409 mean_circ: 0.950927
Ran for 83050 frames Cell Events: 4458 mean_circ: 0.950913
Ran for 83550 frames Cell Events: 4484 mean_circ: 0.950821
Ran for 84050 frames Cell Events: 4508 mean_circ: 0.950803
Ran for 84550 frames Cell Events: 4545 mean_circ: 0.95083
Ran for 85050 frames Cell Events: 4571 mean_circ: 0.950805
Ran for 85550 frames Cell Events: 4608 mean_circ: 0.950817
Ran for 86050 frames Cell Events: 4633 mean_circ: 0.950771
Ran for 87050 frames Cell Events: 4702 mean_circ: 0.950799
Ran for 87550 frames Cell Events: 4733 mean_circ: 0.950853
Ran for 88050 frames Cell Events: 4759 mean_circ: 0.95078
Ran for 89550 frames Cell Events: 4854 mean_circ: 0.950814
Ran for 90050 frames Cell Events: 4879 mean_circ: 0.950804
Ran for 90550 frames Cell Events: 4899 mean_circ: 0.950845
Ran for 91050 frames Cell Events: 4921 mean_circ: 0.950824
Ran for 91550 frames Cell Events: 4952 mean_circ: 0.950847
Ran for 92550 frames Cell Events: 5007 mean_circ: 0.950899
Ran for 93050 frames Cell Events: 5041 mean_circ: 0.950884
Ran for 93550 frames Cell Events: 5064 mean_circ: 0.950954
Ran for 94550 frames Cell Events: 5112 mean_circ: 0.950983
Ran for 95550 frames Cell Events: 5159 mean_circ: 0.951012
Ran for 96550 frames Cell Events: 5200 mean_circ: 0.951047
Ran for 97050 frames Cell Events: 5229 mean_circ: 0.951086
Ran for 97550 frames Cell Events: 5256 mean_circ: 0.951115
Ran for 98050 frames Cell Events: 5279 mean_circ: 0.951124
Ran for 98550 frames Cell Events: 5295 mean_circ: 0.951161
Ran for 99050 frames Cell Events: 5318 mean_circ: 0.951178
Ran for 100050 frames Cell Events: 5368 mean_circ: 0.951152
Ran for 101050 frames Cell Events: 5382 mean_circ: 0.951149
Ran for 102050 frames Cell Events: 5425 mean_circ: 0.951091
Ran for 102550 frames Cell Events: 5455 mean_circ: 0.951137
Ran for 103550 frames Cell Events: 5509 mean_circ: 0.951074
Ran for 104550 frames Cell Events: 5559 mean_circ: 0.95112
Ran for 105550 frames Cell Events: 5612 mean_circ: 0.95107
Ran for 106550 frames Cell Events: 5667 mean_circ: 0.950988
Ran for 107550 frames Cell Events: 5722 mean_circ: 0.951002
Ran for 109550 frames Cell Events: 5841 mean_circ: 0.950951
Ran for 110050 frames Cell Events: 5867 mean_circ: 0.950949
Ran for 110550 frames Cell Events: 5902 mean_circ: 0.950949
Ran for 111050 frames Cell Events: 5920 mean_circ: 0.950902
Ran for 112050 frames Cell Events: 5967 mean_circ: 0.95099
Ran for 113050 frames Cell Events: 6024 mean_circ: 0.950987
Ran for 113550 frames Cell Events: 6058 mean_circ: 0.951018
Ran for 115050 frames Cell Events: 6140 mean_circ: 0.951071
Ran for 116050 frames Cell Events: 6198 mean_circ: 0.951066
Ran for 117050 frames Cell Events: 6263 mean_circ: 0.951035
Ran for 119050 frames Cell Events: 6422 mean_circ: 0.951045
Ran for 119550 frames Cell Events: 6455 mean_circ: 0.951032
Ran for 121050 frames Cell Events: 6549 mean_circ: 0.950984
Ran for 122050 frames Cell Events: 6612 mean_circ: 0.95103
Ran for 124050 frames Cell Events: 6731 mean_circ: 0.9509
Ran for 124550 frames Cell Events: 6765 mean_circ: 0.950909
Ran for 125050 frames Cell Events: 6789 mean_circ: 0.950895
Ran for 126050 frames Cell Events: 6836 mean_circ: 0.950887
Ran for 127050 frames Cell Events: 6901 mean_circ: 0.95088
Ran for 127550 frames Cell Events: 6914 mean_circ: 0.950807
Ran for 128550 frames Cell Events: 6978 mean_circ: 0.950688
Ran for 129550 frames Cell Events: 7037 mean_circ: 0.950682
Ran for 131050 frames Cell Events: 7112 mean_circ: 0.950636
Ran for 131550 frames Cell Events: 7145 mean_circ: 0.950636
Ran for 134050 frames Cell Events: 7318 mean_circ: 0.950569
Ran for 134550 frames Cell Events: 7341 mean_circ: 0.950507
Ran for 135050 frames Cell Events: 7367 mean_circ: 0.95053
Ran for 135550 frames Cell Events: 7388 mean_circ: 0.950541
Ran for 136050 frames Cell Events: 7411 mean_circ: 0.950527
Ran for 138050 frames Cell Events: 7519 mean_circ: 0.95041
Ran for 139550 frames Cell Events: 7606 mean_circ: 0.95048
Ran for 141050 frames Cell Events: 7705 mean_circ: 0.950452
Ran for 142550 frames Cell Events: 7814 mean_circ: 0.950525
Ran for 143550 frames Cell Events: 7862 mean_circ: 0.950436
Ran for 144550 frames Cell Events: 7911 mean_circ: 0.950465
Ran for 145050 frames Cell Events: 7934 mean_circ: 0.950486



End this measurment..



Stopping time: 1.94967e+009
****************************************
Measurement finished

Mean Circularitiy: 0.9505
Mean Area: 642.512
Mean FL-1 intensity: 4383.95
Mean FL-2 intensity: 5018.4
Mean FL-3 intensity: 0

frames processed: 145075
Total Time: 73700.8ms
Number of Runs (new bg img) 0
Frames collected: 147219
Skipped frames: 163791
Rescued Frames: 5091625
Samples DAQ: 36804765
Samples written: 73609374
Samples per Channel: 36804687
FPS: 1997.52

Samples per second: 499379
Expected samples/s: 500000

Events: 8052
Events per second: 109.252
jumped back (PMT): 3920
#Frames read, frequency
# 0 frames :218638
# 1 frames :63197
# 2 frames :2951
# 3 frames :1103
# 4 frames :585
# 5 frames :1783
# 6 frames :1552
# 7 frames :985
# 8 frames :519
# 9 frames :386
# 10 frames :402
# 11 frames :341
# 12 frames :304
# 13 frames :217
# 14 frames :171
# 15 frames :141
# 16 frames :134
# 17 frames :106
# 18 frames :102
# 19 frames :81
# 20 frames :56
# 21 frames :51
# 22 frames :33
# 23 frames :41
# 24 frames :33
# 25 frames :29
# 26 frames :19
# 27 frames :28
# 28 frames :18
# 29 frames :26
# 30 frames :10
# 31 frames :12
# 32 frames :11
# 33 frames :7
# 34 frames :8
# 35 frames :5
# 36 frames :6
# 37 frames :7
# 38 frames :5
# 39 frames :5
# 40 frames :3
# 41 frames :3
# 42 frames :1
# 43 frames :3
# 44 frames :5
# 45 frames :3
# 47 frames :1
# 48 frames :1
# 49 frames :2
# 50 frames :2
# 52 frames :2
# 53 frames :1
# 54 frames :2
# 56 frames :1
# 58 frames :3
# 59 frames :1
# 63 frames :1
# 66 frames :1
# 68 frames :2
# 72 frames :1
# 80 frames :1
# 99 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1732
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0