Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.13.14 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d1c4f20e5dc9c364c7dfc977f5c866d4",
"name": "M1_20170606_2209_dsRNA_Q7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:13:35"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8089 (max 8090) in '8089'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2571
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.95036e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 102 mean_circ: 0.935747
Ran for 2550 frames Cell Events: 151 mean_circ: 0.937007
Ran for 3550 frames Cell Events: 221 mean_circ: 0.937603
Ran for 4050 frames Cell Events: 249 mean_circ: 0.938818
Ran for 5050 frames Cell Events: 311 mean_circ: 0.937691
Ran for 5550 frames Cell Events: 342 mean_circ: 0.938435
Ran for 6050 frames Cell Events: 366 mean_circ: 0.938886
Ran for 8050 frames Cell Events: 515 mean_circ: 0.939876
Ran for 8550 frames Cell Events: 543 mean_circ: 0.940068
Ran for 9050 frames Cell Events: 564 mean_circ: 0.939919
Ran for 9550 frames Cell Events: 577 mean_circ: 0.939432
Ran for 11050 frames Cell Events: 691 mean_circ: 0.937471
Ran for 12050 frames Cell Events: 747 mean_circ: 0.938394
Ran for 12550 frames Cell Events: 779 mean_circ: 0.938245
Ran for 13050 frames Cell Events: 814 mean_circ: 0.93822
Ran for 13550 frames Cell Events: 841 mean_circ: 0.938153
Ran for 15050 frames Cell Events: 951 mean_circ: 0.938137
Ran for 15550 frames Cell Events: 986 mean_circ: 0.938028
Ran for 16550 frames Cell Events: 1055 mean_circ: 0.938049
Ran for 17050 frames Cell Events: 1073 mean_circ: 0.938314
Ran for 17550 frames Cell Events: 1096 mean_circ: 0.938044
Ran for 18050 frames Cell Events: 1125 mean_circ: 0.938026
Ran for 18550 frames Cell Events: 1151 mean_circ: 0.93804
Ran for 20050 frames Cell Events: 1249 mean_circ: 0.937996
Ran for 21550 frames Cell Events: 1345 mean_circ: 0.93845
Ran for 22550 frames Cell Events: 1395 mean_circ: 0.938615
Ran for 23050 frames Cell Events: 1420 mean_circ: 0.938924
Ran for 23550 frames Cell Events: 1440 mean_circ: 0.938716
Ran for 25050 frames Cell Events: 1521 mean_circ: 0.938542
Ran for 25550 frames Cell Events: 1559 mean_circ: 0.93867
Ran for 26050 frames Cell Events: 1583 mean_circ: 0.938815
Ran for 28050 frames Cell Events: 1686 mean_circ: 0.938878
Ran for 30050 frames Cell Events: 1806 mean_circ: 0.939382
Ran for 31050 frames Cell Events: 1853 mean_circ: 0.93915
Ran for 33050 frames Cell Events: 1976 mean_circ: 0.939082
Ran for 33550 frames Cell Events: 2012 mean_circ: 0.939038
Ran for 34050 frames Cell Events: 2050 mean_circ: 0.938841
Ran for 34550 frames Cell Events: 2063 mean_circ: 0.938856
Ran for 36050 frames Cell Events: 2147 mean_circ: 0.938737
Ran for 36550 frames Cell Events: 2194 mean_circ: 0.938775
Ran for 37050 frames Cell Events: 2227 mean_circ: 0.938899
Ran for 38050 frames Cell Events: 2301 mean_circ: 0.938949
Ran for 40550 frames Cell Events: 2472 mean_circ: 0.938787
Ran for 41050 frames Cell Events: 2507 mean_circ: 0.93888
Ran for 43050 frames Cell Events: 2641 mean_circ: 0.938628
Ran for 43550 frames Cell Events: 2670 mean_circ: 0.938668
Ran for 44050 frames Cell Events: 2703 mean_circ: 0.938418
Ran for 45550 frames Cell Events: 2785 mean_circ: 0.938431
Ran for 46050 frames Cell Events: 2819 mean_circ: 0.938444
Ran for 46550 frames Cell Events: 2844 mean_circ: 0.938388
Ran for 47050 frames Cell Events: 2864 mean_circ: 0.93843
Ran for 48550 frames Cell Events: 2948 mean_circ: 0.938417
Ran for 49050 frames Cell Events: 2984 mean_circ: 0.938493
Ran for 49550 frames Cell Events: 3002 mean_circ: 0.938408
Ran for 50050 frames Cell Events: 3027 mean_circ: 0.938489
Ran for 50550 frames Cell Events: 3056 mean_circ: 0.938242
Ran for 51050 frames Cell Events: 3084 mean_circ: 0.938235
Ran for 51550 frames Cell Events: 3117 mean_circ: 0.938359
Ran for 52550 frames Cell Events: 3186 mean_circ: 0.938112
Ran for 53050 frames Cell Events: 3214 mean_circ: 0.938159
Ran for 53550 frames Cell Events: 3238 mean_circ: 0.938231
Ran for 54550 frames Cell Events: 3296 mean_circ: 0.938164
Ran for 55550 frames Cell Events: 3341 mean_circ: 0.938169
Ran for 56550 frames Cell Events: 3396 mean_circ: 0.938135
Ran for 57050 frames Cell Events: 3419 mean_circ: 0.938159
Ran for 57550 frames Cell Events: 3431 mean_circ: 0.938194
Ran for 58050 frames Cell Events: 3464 mean_circ: 0.938056
Ran for 58550 frames Cell Events: 3492 mean_circ: 0.938086
Ran for 59550 frames Cell Events: 3559 mean_circ: 0.938083
Ran for 60050 frames Cell Events: 3577 mean_circ: 0.938074
Ran for 60550 frames Cell Events: 3610 mean_circ: 0.938105
Ran for 61050 frames Cell Events: 3643 mean_circ: 0.938118
Ran for 61550 frames Cell Events: 3665 mean_circ: 0.937903
Ran for 62050 frames Cell Events: 3690 mean_circ: 0.93786
Ran for 62550 frames Cell Events: 3721 mean_circ: 0.937923
Ran for 63550 frames Cell Events: 3787 mean_circ: 0.937769
Ran for 64050 frames Cell Events: 3820 mean_circ: 0.937728
Ran for 65050 frames Cell Events: 3860 mean_circ: 0.937791
Ran for 65550 frames Cell Events: 3889 mean_circ: 0.937727
Ran for 66050 frames Cell Events: 3924 mean_circ: 0.937776
Ran for 66550 frames Cell Events: 3948 mean_circ: 0.937797
Ran for 67050 frames Cell Events: 3974 mean_circ: 0.937861
Ran for 67550 frames Cell Events: 3998 mean_circ: 0.937925
Ran for 68050 frames Cell Events: 4031 mean_circ: 0.937991
Ran for 68550 frames Cell Events: 4063 mean_circ: 0.937991
Ran for 70050 frames Cell Events: 4165 mean_circ: 0.937992
Ran for 70550 frames Cell Events: 4211 mean_circ: 0.937932
Ran for 71550 frames Cell Events: 4287 mean_circ: 0.937561
Ran for 72050 frames Cell Events: 4325 mean_circ: 0.937527
Ran for 75050 frames Cell Events: 4532 mean_circ: 0.937501
Ran for 75550 frames Cell Events: 4556 mean_circ: 0.937455
Ran for 76050 frames Cell Events: 4577 mean_circ: 0.937508
Ran for 79050 frames Cell Events: 4766 mean_circ: 0.937703
Ran for 79550 frames Cell Events: 4795 mean_circ: 0.937707
Ran for 80550 frames Cell Events: 4854 mean_circ: 0.937611
Ran for 81050 frames Cell Events: 4882 mean_circ: 0.937725
Ran for 81550 frames Cell Events: 4913 mean_circ: 0.937744
Ran for 82050 frames Cell Events: 4938 mean_circ: 0.937759
Ran for 83050 frames Cell Events: 5005 mean_circ: 0.937579
Ran for 84550 frames Cell Events: 5090 mean_circ: 0.937582
Ran for 86050 frames Cell Events: 5196 mean_circ: 0.937548
Ran for 86550 frames Cell Events: 5234 mean_circ: 0.937595
Ran for 87050 frames Cell Events: 5266 mean_circ: 0.93752
Ran for 87550 frames Cell Events: 5296 mean_circ: 0.937501
Ran for 88050 frames Cell Events: 5327 mean_circ: 0.937472
Ran for 89550 frames Cell Events: 5413 mean_circ: 0.937481
Ran for 90550 frames Cell Events: 5483 mean_circ: 0.93753
Ran for 91050 frames Cell Events: 5526 mean_circ: 0.937529
Ran for 92050 frames Cell Events: 5594 mean_circ: 0.937616
Ran for 92550 frames Cell Events: 5618 mean_circ: 0.937627
Ran for 93050 frames Cell Events: 5656 mean_circ: 0.937675
Ran for 94550 frames Cell Events: 5760 mean_circ: 0.937574
Ran for 95550 frames Cell Events: 5824 mean_circ: 0.937648
Ran for 96050 frames Cell Events: 5844 mean_circ: 0.937629
Ran for 97050 frames Cell Events: 5897 mean_circ: 0.937651
Ran for 98050 frames Cell Events: 5937 mean_circ: 0.937719
Ran for 98550 frames Cell Events: 5964 mean_circ: 0.937764
Ran for 99050 frames Cell Events: 6003 mean_circ: 0.937769
Ran for 99550 frames Cell Events: 6036 mean_circ: 0.937752
Ran for 100050 frames Cell Events: 6086 mean_circ: 0.937741
Ran for 100550 frames Cell Events: 6109 mean_circ: 0.937777
Ran for 101050 frames Cell Events: 6131 mean_circ: 0.937761
Ran for 102050 frames Cell Events: 6186 mean_circ: 0.937843
Ran for 102550 frames Cell Events: 6217 mean_circ: 0.937855
Ran for 103550 frames Cell Events: 6277 mean_circ: 0.937899
Ran for 105050 frames Cell Events: 6393 mean_circ: 0.93792
Ran for 106050 frames Cell Events: 6456 mean_circ: 0.937983
Ran for 107050 frames Cell Events: 6515 mean_circ: 0.937995
Ran for 107550 frames Cell Events: 6538 mean_circ: 0.938014
Ran for 108050 frames Cell Events: 6563 mean_circ: 0.938039
Ran for 108550 frames Cell Events: 6588 mean_circ: 0.938035
Ran for 109050 frames Cell Events: 6611 mean_circ: 0.938052
Ran for 109550 frames Cell Events: 6624 mean_circ: 0.938098
Ran for 110050 frames Cell Events: 6647 mean_circ: 0.938039
Ran for 110550 frames Cell Events: 6674 mean_circ: 0.938097
Ran for 111050 frames Cell Events: 6694 mean_circ: 0.938058
Ran for 113550 frames Cell Events: 6847 mean_circ: 0.937881
Ran for 114050 frames Cell Events: 6872 mean_circ: 0.937887
Ran for 114550 frames Cell Events: 6903 mean_circ: 0.937929
Ran for 115050 frames Cell Events: 6945 mean_circ: 0.937963
Ran for 115550 frames Cell Events: 6981 mean_circ: 0.937961
Ran for 116050 frames Cell Events: 7021 mean_circ: 0.937947
Ran for 116550 frames Cell Events: 7052 mean_circ: 0.937904
Ran for 118050 frames Cell Events: 7117 mean_circ: 0.937961
Ran for 118550 frames Cell Events: 7151 mean_circ: 0.937977
Ran for 121550 frames Cell Events: 7357 mean_circ: 0.937923
Ran for 122050 frames Cell Events: 7385 mean_circ: 0.937918
Ran for 122550 frames Cell Events: 7417 mean_circ: 0.937885
Ran for 123050 frames Cell Events: 7446 mean_circ: 0.93793
Ran for 124050 frames Cell Events: 7492 mean_circ: 0.937925
Ran for 127050 frames Cell Events: 7682 mean_circ: 0.937811
Ran for 127550 frames Cell Events: 7715 mean_circ: 0.937822
Ran for 128050 frames Cell Events: 7741 mean_circ: 0.937785
Ran for 128550 frames Cell Events: 7768 mean_circ: 0.937745
Ran for 129550 frames Cell Events: 7826 mean_circ: 0.937742
Ran for 130050 frames Cell Events: 7861 mean_circ: 0.937775
Ran for 131050 frames Cell Events: 7946 mean_circ: 0.937738
Ran for 132050 frames Cell Events: 8033 mean_circ: 0.93771



End this measurment..



Stopping time: 1.95043e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93772
Mean Area: 643.724
Mean FL-1 intensity: 4307.28
Mean FL-2 intensity: 5696.09
Mean FL-3 intensity: 0

frames processed: 131669
Total Time: 66494.6ms
Number of Runs (new bg img) 0
Frames collected: 132811
Skipped frames: 167804
Rescued Frames: 5291020
Samples DAQ: 33205036
Samples written: 66405222
Samples per Channel: 33202611
FPS: 1997.32

Samples per second: 499328
Expected samples/s: 500000

Events: 8090
Events per second: 121.664
jumped back (PMT): 27
#Frames read, frequency
# 0 frames :201320
# 1 frames :51772
# 2 frames :2853
# 3 frames :1168
# 4 frames :532
# 5 frames :2054
# 6 frames :1218
# 7 frames :1027
# 8 frames :548
# 9 frames :389
# 10 frames :708
# 11 frames :356
# 12 frames :302
# 13 frames :228
# 14 frames :208
# 15 frames :140
# 16 frames :124
# 17 frames :75
# 18 frames :72
# 19 frames :69
# 20 frames :47
# 21 frames :32
# 22 frames :37
# 23 frames :24
# 24 frames :24
# 25 frames :19
# 26 frames :20
# 27 frames :13
# 28 frames :11
# 29 frames :15
# 30 frames :11
# 31 frames :9
# 32 frames :7
# 33 frames :1
# 34 frames :2
# 35 frames :3
# 36 frames :6
# 37 frames :1
# 38 frames :2
# 40 frames :2
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 44 frames :2
# 45 frames :1
# 46 frames :1
# 49 frames :1
# 51 frames :1
# 55 frames :1
# 71 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2571
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0