Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.17.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d62790f23c7569294fa95a31abb91eb0",
"name": "M1_20170608_1358_dsRNA_T4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:17:52"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8487 (max 8488) in '8487'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7319
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09373e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 3550 frames Cell Events: 175 mean_circ: 0.941799
Ran for 4050 frames Cell Events: 191 mean_circ: 0.942775
Ran for 4550 frames Cell Events: 212 mean_circ: 0.942668
Ran for 5050 frames Cell Events: 226 mean_circ: 0.942373
Ran for 7050 frames Cell Events: 301 mean_circ: 0.944241
Ran for 8050 frames Cell Events: 346 mean_circ: 0.945658
Ran for 8550 frames Cell Events: 359 mean_circ: 0.946166
Ran for 10050 frames Cell Events: 420 mean_circ: 0.945802
Ran for 10550 frames Cell Events: 438 mean_circ: 0.945839
Ran for 12050 frames Cell Events: 490 mean_circ: 0.946196
Ran for 12550 frames Cell Events: 515 mean_circ: 0.946728
Ran for 14550 frames Cell Events: 590 mean_circ: 0.946279
Ran for 15050 frames Cell Events: 615 mean_circ: 0.946107
Ran for 16050 frames Cell Events: 655 mean_circ: 0.945734
Ran for 16550 frames Cell Events: 678 mean_circ: 0.945731
Ran for 17550 frames Cell Events: 722 mean_circ: 0.944769
Ran for 18050 frames Cell Events: 740 mean_circ: 0.944996
Ran for 19050 frames Cell Events: 781 mean_circ: 0.9447
Ran for 19550 frames Cell Events: 797 mean_circ: 0.944686
Ran for 22050 frames Cell Events: 921 mean_circ: 0.944233
Ran for 23050 frames Cell Events: 954 mean_circ: 0.943803
Ran for 25550 frames Cell Events: 1047 mean_circ: 0.943576
Ran for 27050 frames Cell Events: 1115 mean_circ: 0.943375
Ran for 28550 frames Cell Events: 1176 mean_circ: 0.943692
Ran for 29550 frames Cell Events: 1204 mean_circ: 0.943639
Ran for 30050 frames Cell Events: 1218 mean_circ: 0.943765
Ran for 30550 frames Cell Events: 1230 mean_circ: 0.943656
Ran for 31050 frames Cell Events: 1245 mean_circ: 0.943689
Ran for 31550 frames Cell Events: 1258 mean_circ: 0.943634
Ran for 32050 frames Cell Events: 1280 mean_circ: 0.943657
Ran for 32550 frames Cell Events: 1305 mean_circ: 0.943325
Ran for 33550 frames Cell Events: 1355 mean_circ: 0.943439
Ran for 38050 frames Cell Events: 1565 mean_circ: 0.944084
Ran for 38550 frames Cell Events: 1577 mean_circ: 0.944207
Ran for 40050 frames Cell Events: 1630 mean_circ: 0.944253
Ran for 42550 frames Cell Events: 1760 mean_circ: 0.943891
Ran for 44050 frames Cell Events: 1835 mean_circ: 0.943744
Ran for 45550 frames Cell Events: 1897 mean_circ: 0.943778
Ran for 46050 frames Cell Events: 1913 mean_circ: 0.944104
Ran for 46550 frames Cell Events: 1934 mean_circ: 0.944094
Ran for 47550 frames Cell Events: 1981 mean_circ: 0.944199
Ran for 49050 frames Cell Events: 2029 mean_circ: 0.944026
Ran for 50550 frames Cell Events: 2093 mean_circ: 0.943809
Ran for 53050 frames Cell Events: 2206 mean_circ: 0.943938
Ran for 54050 frames Cell Events: 2257 mean_circ: 0.943976
Ran for 54550 frames Cell Events: 2283 mean_circ: 0.943984
Ran for 55050 frames Cell Events: 2300 mean_circ: 0.944067
Ran for 56550 frames Cell Events: 2368 mean_circ: 0.944273
Ran for 60550 frames Cell Events: 2550 mean_circ: 0.944258
Ran for 61050 frames Cell Events: 2570 mean_circ: 0.944249
Ran for 61550 frames Cell Events: 2591 mean_circ: 0.944234
Ran for 62550 frames Cell Events: 2633 mean_circ: 0.944082
Ran for 64050 frames Cell Events: 2698 mean_circ: 0.944276
Ran for 65050 frames Cell Events: 2739 mean_circ: 0.94436
Ran for 65550 frames Cell Events: 2762 mean_circ: 0.944377
Ran for 66050 frames Cell Events: 2778 mean_circ: 0.944399
Ran for 68050 frames Cell Events: 2865 mean_circ: 0.944373
Ran for 68550 frames Cell Events: 2891 mean_circ: 0.944254
Ran for 69550 frames Cell Events: 2934 mean_circ: 0.94429
Ran for 70550 frames Cell Events: 2985 mean_circ: 0.944295
Ran for 71050 frames Cell Events: 3017 mean_circ: 0.944295
Ran for 71550 frames Cell Events: 3036 mean_circ: 0.944242
Ran for 73050 frames Cell Events: 3108 mean_circ: 0.944279
Ran for 74050 frames Cell Events: 3144 mean_circ: 0.944475
Ran for 75550 frames Cell Events: 3212 mean_circ: 0.944476
Ran for 77550 frames Cell Events: 3307 mean_circ: 0.944462
Ran for 78550 frames Cell Events: 3359 mean_circ: 0.944177
Ran for 81050 frames Cell Events: 3472 mean_circ: 0.944022
Ran for 81550 frames Cell Events: 3495 mean_circ: 0.943965
Ran for 82050 frames Cell Events: 3519 mean_circ: 0.943995
Ran for 83550 frames Cell Events: 3577 mean_circ: 0.943986
Ran for 84550 frames Cell Events: 3623 mean_circ: 0.944047
Ran for 86050 frames Cell Events: 3697 mean_circ: 0.944095
Ran for 87050 frames Cell Events: 3743 mean_circ: 0.944228
Ran for 87550 frames Cell Events: 3764 mean_circ: 0.944287
Ran for 89050 frames Cell Events: 3831 mean_circ: 0.944372
Ran for 92550 frames Cell Events: 3982 mean_circ: 0.944305
Ran for 94550 frames Cell Events: 4076 mean_circ: 0.944226
Ran for 95550 frames Cell Events: 4129 mean_circ: 0.944199
Ran for 96550 frames Cell Events: 4179 mean_circ: 0.944214
Ran for 97550 frames Cell Events: 4220 mean_circ: 0.944279
Ran for 98050 frames Cell Events: 4246 mean_circ: 0.944226
Ran for 99050 frames Cell Events: 4298 mean_circ: 0.944257
Ran for 100050 frames Cell Events: 4341 mean_circ: 0.944138
Ran for 102550 frames Cell Events: 4481 mean_circ: 0.944174
Ran for 103050 frames Cell Events: 4507 mean_circ: 0.944194
Ran for 103550 frames Cell Events: 4532 mean_circ: 0.944254
Ran for 104050 frames Cell Events: 4558 mean_circ: 0.944254
Ran for 104550 frames Cell Events: 4596 mean_circ: 0.944226
Ran for 106050 frames Cell Events: 4670 mean_circ: 0.944163
Ran for 108050 frames Cell Events: 4767 mean_circ: 0.944248
Ran for 108550 frames Cell Events: 4777 mean_circ: 0.944275
Ran for 109050 frames Cell Events: 4794 mean_circ: 0.944283
Ran for 110550 frames Cell Events: 4867 mean_circ: 0.9444
Ran for 111550 frames Cell Events: 4900 mean_circ: 0.944396
Ran for 113050 frames Cell Events: 4958 mean_circ: 0.944416
Ran for 113550 frames Cell Events: 4972 mean_circ: 0.944425
Ran for 114050 frames Cell Events: 4987 mean_circ: 0.944461
Ran for 118050 frames Cell Events: 5192 mean_circ: 0.944525
Ran for 120550 frames Cell Events: 5313 mean_circ: 0.944688
Ran for 124550 frames Cell Events: 5482 mean_circ: 0.944689
Ran for 126050 frames Cell Events: 5553 mean_circ: 0.944701
Ran for 128050 frames Cell Events: 5628 mean_circ: 0.944712
Ran for 128550 frames Cell Events: 5657 mean_circ: 0.944786
Ran for 130550 frames Cell Events: 5765 mean_circ: 0.944771
Ran for 131050 frames Cell Events: 5782 mean_circ: 0.944814
Ran for 132550 frames Cell Events: 5871 mean_circ: 0.94484
Ran for 133050 frames Cell Events: 5899 mean_circ: 0.944793
Ran for 135050 frames Cell Events: 5999 mean_circ: 0.944769
Ran for 135550 frames Cell Events: 6024 mean_circ: 0.944732
Ran for 136550 frames Cell Events: 6079 mean_circ: 0.944646
Ran for 138550 frames Cell Events: 6160 mean_circ: 0.944718
Ran for 139050 frames Cell Events: 6174 mean_circ: 0.944735
Ran for 139550 frames Cell Events: 6188 mean_circ: 0.94472
Ran for 140050 frames Cell Events: 6200 mean_circ: 0.944717
Ran for 140550 frames Cell Events: 6215 mean_circ: 0.944705
Ran for 141550 frames Cell Events: 6252 mean_circ: 0.944634
Ran for 142550 frames Cell Events: 6295 mean_circ: 0.944605
Ran for 143050 frames Cell Events: 6327 mean_circ: 0.944637
Ran for 144050 frames Cell Events: 6372 mean_circ: 0.944733
Ran for 144550 frames Cell Events: 6400 mean_circ: 0.944707
Ran for 145050 frames Cell Events: 6429 mean_circ: 0.944694
Ran for 146050 frames Cell Events: 6475 mean_circ: 0.944721
Ran for 147050 frames Cell Events: 6525 mean_circ: 0.944674
Ran for 148050 frames Cell Events: 6569 mean_circ: 0.944675
Ran for 148550 frames Cell Events: 6595 mean_circ: 0.944652
Ran for 152050 frames Cell Events: 6768 mean_circ: 0.944732
Ran for 152550 frames Cell Events: 6792 mean_circ: 0.944733
Ran for 155050 frames Cell Events: 6901 mean_circ: 0.944768
Ran for 156050 frames Cell Events: 6924 mean_circ: 0.944777
Ran for 157050 frames Cell Events: 6971 mean_circ: 0.944778
Ran for 159050 frames Cell Events: 7051 mean_circ: 0.944821
Ran for 161550 frames Cell Events: 7141 mean_circ: 0.944897
Ran for 162050 frames Cell Events: 7164 mean_circ: 0.944926
Ran for 163550 frames Cell Events: 7225 mean_circ: 0.944953
Ran for 164050 frames Cell Events: 7238 mean_circ: 0.944965
Ran for 166550 frames Cell Events: 7357 mean_circ: 0.944996
Ran for 167550 frames Cell Events: 7403 mean_circ: 0.944902
Ran for 169550 frames Cell Events: 7500 mean_circ: 0.944945
Ran for 170550 frames Cell Events: 7553 mean_circ: 0.944971
Ran for 172050 frames Cell Events: 7628 mean_circ: 0.945011
Ran for 172550 frames Cell Events: 7644 mean_circ: 0.945038
Ran for 174050 frames Cell Events: 7705 mean_circ: 0.945086
Ran for 174550 frames Cell Events: 7724 mean_circ: 0.945079
Ran for 175050 frames Cell Events: 7751 mean_circ: 0.94508
Ran for 176050 frames Cell Events: 7802 mean_circ: 0.945068
Ran for 176550 frames Cell Events: 7822 mean_circ: 0.945046
Ran for 179550 frames Cell Events: 7979 mean_circ: 0.945104
Ran for 180050 frames Cell Events: 7999 mean_circ: 0.945105
Ran for 180550 frames Cell Events: 8023 mean_circ: 0.945103
Ran for 181050 frames Cell Events: 8047 mean_circ: 0.945068
Ran for 183050 frames Cell Events: 8141 mean_circ: 0.945009
Ran for 184050 frames Cell Events: 8188 mean_circ: 0.945029
Ran for 185050 frames Cell Events: 8233 mean_circ: 0.945036
Ran for 185550 frames Cell Events: 8252 mean_circ: 0.945044
Ran for 186550 frames Cell Events: 8295 mean_circ: 0.945069
Ran for 189050 frames Cell Events: 8406 mean_circ: 0.945103
Ran for 190550 frames Cell Events: 8456 mean_circ: 0.945079



End this measurment..



Stopping time: 2.09383e+009
****************************************
Measurement finished

Mean Circularitiy: 0.945058
Mean Area: 649.681
Mean FL-1 intensity: 4118.84
Mean FL-2 intensity: 4706.14
Mean FL-3 intensity: 0

frames processed: 186581
Total Time: 95765.4ms
Number of Runs (new bg img) 0
Frames collected: 191406
Skipped frames: 19138
Rescued Frames: 980848
Samples DAQ: 47853199
Samples written: 95703006
Samples per Channel: 47851503
FPS: 1998.7

Samples per second: 499674
Expected samples/s: 500000

Events: 8488
Events per second: 88.6333
jumped back (PMT): 3074
#Frames read, frequency
# 0 frames :310478
# 1 frames :58407
# 2 frames :2591
# 3 frames :1453
# 4 frames :884
# 5 frames :568
# 6 frames :526
# 7 frames :990
# 8 frames :913
# 9 frames :833
# 10 frames :669
# 11 frames :600
# 12 frames :544
# 13 frames :386
# 14 frames :285
# 15 frames :236
# 16 frames :217
# 17 frames :223
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# 19 frames :223
# 20 frames :170
# 21 frames :149
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# 25 frames :85
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# 97 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7319
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0