Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.17.59 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0d96bcb808fe0bc9f3e5dd28b1c13fc4",
"name": "M1_20170608_1402_dsRNA_T4_fast.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:18:19"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8093 (max 8094) in '8093'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7219
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09393e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 57 mean_circ: 0.933286
Ran for 1550 frames Cell Events: 78 mean_circ: 0.935627
Ran for 3550 frames Cell Events: 167 mean_circ: 0.935991
Ran for 4050 frames Cell Events: 190 mean_circ: 0.936758
Ran for 4550 frames Cell Events: 213 mean_circ: 0.937383
Ran for 5550 frames Cell Events: 253 mean_circ: 0.937279
Ran for 6050 frames Cell Events: 281 mean_circ: 0.937044
Ran for 7550 frames Cell Events: 373 mean_circ: 0.937333
Ran for 8050 frames Cell Events: 388 mean_circ: 0.93736
Ran for 10050 frames Cell Events: 490 mean_circ: 0.936854
Ran for 11050 frames Cell Events: 551 mean_circ: 0.936588
Ran for 11550 frames Cell Events: 582 mean_circ: 0.936691
Ran for 12050 frames Cell Events: 612 mean_circ: 0.936047
Ran for 12550 frames Cell Events: 622 mean_circ: 0.936154
Ran for 13550 frames Cell Events: 665 mean_circ: 0.93599
Ran for 14050 frames Cell Events: 690 mean_circ: 0.935954
Ran for 14550 frames Cell Events: 711 mean_circ: 0.936329
Ran for 16050 frames Cell Events: 810 mean_circ: 0.937086
Ran for 17550 frames Cell Events: 896 mean_circ: 0.936317
Ran for 18050 frames Cell Events: 929 mean_circ: 0.936699
Ran for 19550 frames Cell Events: 995 mean_circ: 0.936283
Ran for 20050 frames Cell Events: 1014 mean_circ: 0.935869
Ran for 21550 frames Cell Events: 1086 mean_circ: 0.935838
Ran for 22050 frames Cell Events: 1105 mean_circ: 0.935725
Ran for 24050 frames Cell Events: 1197 mean_circ: 0.935852
Ran for 25050 frames Cell Events: 1242 mean_circ: 0.936059
Ran for 25550 frames Cell Events: 1267 mean_circ: 0.935703
Ran for 26050 frames Cell Events: 1290 mean_circ: 0.935631
Ran for 27050 frames Cell Events: 1351 mean_circ: 0.93562
Ran for 27550 frames Cell Events: 1364 mean_circ: 0.935837
Ran for 28050 frames Cell Events: 1382 mean_circ: 0.935865
Ran for 28550 frames Cell Events: 1405 mean_circ: 0.935677
Ran for 29550 frames Cell Events: 1457 mean_circ: 0.935674
Ran for 30550 frames Cell Events: 1503 mean_circ: 0.935668
Ran for 34050 frames Cell Events: 1686 mean_circ: 0.935241
Ran for 34550 frames Cell Events: 1704 mean_circ: 0.935326
Ran for 35550 frames Cell Events: 1757 mean_circ: 0.935228
Ran for 36050 frames Cell Events: 1780 mean_circ: 0.934973
Ran for 37050 frames Cell Events: 1839 mean_circ: 0.935116
Ran for 39050 frames Cell Events: 1969 mean_circ: 0.935088
Ran for 40050 frames Cell Events: 2018 mean_circ: 0.935065
Ran for 41050 frames Cell Events: 2049 mean_circ: 0.93505
Ran for 42050 frames Cell Events: 2085 mean_circ: 0.934968
Ran for 42550 frames Cell Events: 2102 mean_circ: 0.93499
Ran for 43050 frames Cell Events: 2123 mean_circ: 0.93499
Ran for 43550 frames Cell Events: 2148 mean_circ: 0.934975
Ran for 44050 frames Cell Events: 2174 mean_circ: 0.934879
Ran for 44550 frames Cell Events: 2200 mean_circ: 0.934799
Ran for 45050 frames Cell Events: 2229 mean_circ: 0.934786
Ran for 45550 frames Cell Events: 2263 mean_circ: 0.934828
Ran for 47050 frames Cell Events: 2340 mean_circ: 0.934902
Ran for 48550 frames Cell Events: 2444 mean_circ: 0.934894
Ran for 49050 frames Cell Events: 2472 mean_circ: 0.934749
Ran for 50550 frames Cell Events: 2546 mean_circ: 0.935208
Ran for 51050 frames Cell Events: 2565 mean_circ: 0.935263
Ran for 52050 frames Cell Events: 2628 mean_circ: 0.935227
Ran for 54050 frames Cell Events: 2758 mean_circ: 0.935195
Ran for 54550 frames Cell Events: 2783 mean_circ: 0.935111
Ran for 55050 frames Cell Events: 2810 mean_circ: 0.934971
Ran for 55550 frames Cell Events: 2835 mean_circ: 0.93504
Ran for 56550 frames Cell Events: 2897 mean_circ: 0.935123
Ran for 57050 frames Cell Events: 2917 mean_circ: 0.93512
Ran for 57550 frames Cell Events: 2933 mean_circ: 0.935119
Ran for 58050 frames Cell Events: 2956 mean_circ: 0.935115
Ran for 59550 frames Cell Events: 3036 mean_circ: 0.935117
Ran for 60050 frames Cell Events: 3065 mean_circ: 0.935151
Ran for 60550 frames Cell Events: 3095 mean_circ: 0.935289
Ran for 61050 frames Cell Events: 3116 mean_circ: 0.935333
Ran for 62050 frames Cell Events: 3164 mean_circ: 0.935368
Ran for 63050 frames Cell Events: 3208 mean_circ: 0.935447
Ran for 64050 frames Cell Events: 3246 mean_circ: 0.935371
Ran for 64550 frames Cell Events: 3280 mean_circ: 0.935474
Ran for 65050 frames Cell Events: 3306 mean_circ: 0.935486
Ran for 65550 frames Cell Events: 3340 mean_circ: 0.935599
Ran for 66550 frames Cell Events: 3394 mean_circ: 0.935678
Ran for 67050 frames Cell Events: 3420 mean_circ: 0.93569
Ran for 67550 frames Cell Events: 3433 mean_circ: 0.935693
Ran for 68050 frames Cell Events: 3450 mean_circ: 0.935703
Ran for 68550 frames Cell Events: 3474 mean_circ: 0.935647
Ran for 69550 frames Cell Events: 3520 mean_circ: 0.93563
Ran for 70050 frames Cell Events: 3531 mean_circ: 0.93562
Ran for 71050 frames Cell Events: 3578 mean_circ: 0.935665
Ran for 71550 frames Cell Events: 3600 mean_circ: 0.935621
Ran for 73550 frames Cell Events: 3721 mean_circ: 0.935709
Ran for 75050 frames Cell Events: 3807 mean_circ: 0.935698
Ran for 75550 frames Cell Events: 3825 mean_circ: 0.935623
Ran for 76050 frames Cell Events: 3852 mean_circ: 0.935555
Ran for 77050 frames Cell Events: 3905 mean_circ: 0.93555
Ran for 77550 frames Cell Events: 3934 mean_circ: 0.935476
Ran for 78050 frames Cell Events: 3951 mean_circ: 0.935551
Ran for 78550 frames Cell Events: 3970 mean_circ: 0.935585
Ran for 79550 frames Cell Events: 4026 mean_circ: 0.935693
Ran for 80050 frames Cell Events: 4044 mean_circ: 0.935785
Ran for 80550 frames Cell Events: 4085 mean_circ: 0.935785
Ran for 83550 frames Cell Events: 4243 mean_circ: 0.935616
Ran for 84550 frames Cell Events: 4297 mean_circ: 0.935629
Ran for 86550 frames Cell Events: 4409 mean_circ: 0.935664
Ran for 87050 frames Cell Events: 4441 mean_circ: 0.935618
Ran for 87550 frames Cell Events: 4476 mean_circ: 0.93564
Ran for 88050 frames Cell Events: 4492 mean_circ: 0.935598
Ran for 89050 frames Cell Events: 4540 mean_circ: 0.935559
Ran for 90550 frames Cell Events: 4614 mean_circ: 0.935594
Ran for 91550 frames Cell Events: 4662 mean_circ: 0.935493
Ran for 92050 frames Cell Events: 4687 mean_circ: 0.935494
Ran for 92550 frames Cell Events: 4706 mean_circ: 0.935416
Ran for 93050 frames Cell Events: 4733 mean_circ: 0.935478
Ran for 93550 frames Cell Events: 4760 mean_circ: 0.93553
Ran for 94550 frames Cell Events: 4806 mean_circ: 0.935547
Ran for 95050 frames Cell Events: 4824 mean_circ: 0.935549
Ran for 96050 frames Cell Events: 4880 mean_circ: 0.935502
Ran for 98050 frames Cell Events: 4989 mean_circ: 0.935373
Ran for 100050 frames Cell Events: 5110 mean_circ: 0.93529
Ran for 100550 frames Cell Events: 5134 mean_circ: 0.935265
Ran for 102050 frames Cell Events: 5214 mean_circ: 0.935346
Ran for 103050 frames Cell Events: 5260 mean_circ: 0.935312
Ran for 104050 frames Cell Events: 5334 mean_circ: 0.935188
Ran for 104550 frames Cell Events: 5358 mean_circ: 0.935211
Ran for 105050 frames Cell Events: 5374 mean_circ: 0.935217
Ran for 106050 frames Cell Events: 5416 mean_circ: 0.935174
Ran for 107050 frames Cell Events: 5475 mean_circ: 0.935171
Ran for 107550 frames Cell Events: 5498 mean_circ: 0.935203
Ran for 108050 frames Cell Events: 5527 mean_circ: 0.935203
Ran for 108550 frames Cell Events: 5540 mean_circ: 0.93528
Ran for 109550 frames Cell Events: 5601 mean_circ: 0.935283
Ran for 111050 frames Cell Events: 5664 mean_circ: 0.935163
Ran for 111550 frames Cell Events: 5682 mean_circ: 0.935159
Ran for 112550 frames Cell Events: 5714 mean_circ: 0.935108
Ran for 113050 frames Cell Events: 5728 mean_circ: 0.935115
Ran for 113550 frames Cell Events: 5768 mean_circ: 0.935161
Ran for 114050 frames Cell Events: 5807 mean_circ: 0.935083
Ran for 115050 frames Cell Events: 5870 mean_circ: 0.935124
Ran for 116050 frames Cell Events: 5922 mean_circ: 0.935104
Ran for 118050 frames Cell Events: 6021 mean_circ: 0.935107
Ran for 119050 frames Cell Events: 6080 mean_circ: 0.935133
Ran for 120550 frames Cell Events: 6180 mean_circ: 0.935051
Ran for 121550 frames Cell Events: 6225 mean_circ: 0.935114
Ran for 122050 frames Cell Events: 6237 mean_circ: 0.935095
Ran for 123050 frames Cell Events: 6281 mean_circ: 0.935037
Ran for 124550 frames Cell Events: 6337 mean_circ: 0.934982
Ran for 125050 frames Cell Events: 6354 mean_circ: 0.935057
Ran for 126050 frames Cell Events: 6403 mean_circ: 0.935054
Ran for 126550 frames Cell Events: 6430 mean_circ: 0.935057
Ran for 128550 frames Cell Events: 6551 mean_circ: 0.934845
Ran for 129050 frames Cell Events: 6577 mean_circ: 0.934813
Ran for 130550 frames Cell Events: 6654 mean_circ: 0.934868
Ran for 131050 frames Cell Events: 6672 mean_circ: 0.934891
Ran for 131550 frames Cell Events: 6691 mean_circ: 0.934946
Ran for 132550 frames Cell Events: 6737 mean_circ: 0.934944
Ran for 134050 frames Cell Events: 6794 mean_circ: 0.934976
Ran for 135050 frames Cell Events: 6836 mean_circ: 0.934984
Ran for 136550 frames Cell Events: 6908 mean_circ: 0.935015
Ran for 137050 frames Cell Events: 6931 mean_circ: 0.935001
Ran for 137550 frames Cell Events: 6943 mean_circ: 0.935033
Ran for 139050 frames Cell Events: 7022 mean_circ: 0.934911
Ran for 139550 frames Cell Events: 7043 mean_circ: 0.934893
Ran for 140050 frames Cell Events: 7064 mean_circ: 0.934889
Ran for 141550 frames Cell Events: 7132 mean_circ: 0.934896
Ran for 142050 frames Cell Events: 7149 mean_circ: 0.934896
Ran for 145050 frames Cell Events: 7308 mean_circ: 0.934738
Ran for 145550 frames Cell Events: 7327 mean_circ: 0.934748
Ran for 146050 frames Cell Events: 7344 mean_circ: 0.934782
Ran for 146550 frames Cell Events: 7373 mean_circ: 0.934799
Ran for 147550 frames Cell Events: 7431 mean_circ: 0.934775
Ran for 148050 frames Cell Events: 7461 mean_circ: 0.934813
Ran for 148550 frames Cell Events: 7494 mean_circ: 0.934703
Ran for 149050 frames Cell Events: 7515 mean_circ: 0.934717
Ran for 150550 frames Cell Events: 7602 mean_circ: 0.934675
Ran for 151050 frames Cell Events: 7627 mean_circ: 0.934666
Ran for 152050 frames Cell Events: 7664 mean_circ: 0.934697
Ran for 152550 frames Cell Events: 7689 mean_circ: 0.934713
Ran for 153050 frames Cell Events: 7712 mean_circ: 0.934723
Ran for 153550 frames Cell Events: 7727 mean_circ: 0.934718
Ran for 154050 frames Cell Events: 7750 mean_circ: 0.934704
Ran for 155550 frames Cell Events: 7825 mean_circ: 0.934598
Ran for 157050 frames Cell Events: 7934 mean_circ: 0.934583
Ran for 158050 frames Cell Events: 7993 mean_circ: 0.934568
Ran for 159550 frames Cell Events: 8088 mean_circ: 0.934592



End this measurment..



Stopping time: 2.09401e+009
****************************************
Measurement finished

Mean Circularitiy: 0.934607
Mean Area: 651.552
Mean FL-1 intensity: 3849.93
Mean FL-2 intensity: 4883.58
Mean FL-3 intensity: 0

frames processed: 159048
Total Time: 79905.1ms
Number of Runs (new bg img) 0
Frames collected: 159664
Skipped frames: 19754
Rescued Frames: 1065244
Samples DAQ: 39916059
Samples written: 79832036
Samples per Channel: 39916018
FPS: 1998.17

Samples per second: 499542
Expected samples/s: 500000

Events: 8094
Events per second: 101.295
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :244533
# 1 frames :61230
# 2 frames :3258
# 3 frames :1313
# 4 frames :788
# 5 frames :359
# 6 frames :1367
# 7 frames :1165
# 8 frames :954
# 9 frames :674
# 10 frames :793
# 11 frames :613
# 12 frames :426
# 13 frames :203
# 14 frames :198
# 15 frames :170
# 16 frames :192
# 17 frames :164
# 18 frames :112
# 19 frames :97
# 20 frames :88
# 21 frames :78
# 22 frames :57
# 23 frames :51
# 24 frames :38
# 25 frames :33
# 26 frames :32
# 27 frames :27
# 28 frames :27
# 29 frames :24
# 30 frames :20
# 31 frames :18
# 32 frames :9
# 33 frames :11
# 34 frames :6
# 35 frames :4
# 36 frames :8
# 37 frames :6
# 38 frames :5
# 39 frames :3
# 40 frames :4
# 41 frames :10
# 42 frames :1
# 43 frames :2
# 44 frames :2
# 45 frames :3
# 46 frames :2
# 47 frames :3
# 48 frames :1
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 52 frames :3
# 54 frames :3
# 56 frames :1
# 57 frames :1
# 63 frames :1
# 71 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7219
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0