Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.20.58 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2f04eefde75c6b2fd7f4cf7c42353ee2",
"name": "M1_20170608_1437_dsRNA_T7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:21:20"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8310 (max 8311) in '8310'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6216
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09609e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.949628
Ran for 1050 frames Cell Events: 37 mean_circ: 0.942515
Ran for 2050 frames Cell Events: 68 mean_circ: 0.945078
Ran for 4550 frames Cell Events: 168 mean_circ: 0.943111
Ran for 5050 frames Cell Events: 184 mean_circ: 0.941997
Ran for 5550 frames Cell Events: 206 mean_circ: 0.942698
Ran for 6050 frames Cell Events: 224 mean_circ: 0.9432
Ran for 7050 frames Cell Events: 261 mean_circ: 0.943537
Ran for 7550 frames Cell Events: 282 mean_circ: 0.943653
Ran for 8050 frames Cell Events: 294 mean_circ: 0.943245
Ran for 8550 frames Cell Events: 316 mean_circ: 0.943081
Ran for 9550 frames Cell Events: 358 mean_circ: 0.943023
Ran for 10050 frames Cell Events: 375 mean_circ: 0.942374
Ran for 11050 frames Cell Events: 405 mean_circ: 0.942486
Ran for 12050 frames Cell Events: 441 mean_circ: 0.942324
Ran for 12550 frames Cell Events: 462 mean_circ: 0.942118
Ran for 13050 frames Cell Events: 482 mean_circ: 0.942317
Ran for 14050 frames Cell Events: 524 mean_circ: 0.942573
Ran for 15050 frames Cell Events: 565 mean_circ: 0.942439
Ran for 16050 frames Cell Events: 619 mean_circ: 0.942219
Ran for 17050 frames Cell Events: 649 mean_circ: 0.942281
Ran for 17550 frames Cell Events: 665 mean_circ: 0.942045
Ran for 18050 frames Cell Events: 685 mean_circ: 0.942341
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Ran for 23050 frames Cell Events: 866 mean_circ: 0.942585
Ran for 23550 frames Cell Events: 876 mean_circ: 0.942604
Ran for 25050 frames Cell Events: 936 mean_circ: 0.9424
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Ran for 34550 frames Cell Events: 1313 mean_circ: 0.942108
Ran for 35050 frames Cell Events: 1347 mean_circ: 0.941571
Ran for 35550 frames Cell Events: 1351 mean_circ: 0.941567
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Ran for 36550 frames Cell Events: 1353 mean_circ: 0.941562
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Ran for 37550 frames Cell Events: 1357 mean_circ: 0.941619
Ran for 38050 frames Cell Events: 1359 mean_circ: 0.941645
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Ran for 39050 frames Cell Events: 1372 mean_circ: 0.9416
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Ran for 40050 frames Cell Events: 1382 mean_circ: 0.941648
Ran for 40550 frames Cell Events: 1389 mean_circ: 0.941704
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Ran for 58050 frames Cell Events: 1933 mean_circ: 0.941079
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Ran for 60050 frames Cell Events: 2020 mean_circ: 0.940644
Ran for 60550 frames Cell Events: 2030 mean_circ: 0.940631
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Ran for 63550 frames Cell Events: 2159 mean_circ: 0.939987
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Ran for 64550 frames Cell Events: 2195 mean_circ: 0.939965
Ran for 65050 frames Cell Events: 2211 mean_circ: 0.93998
Ran for 66050 frames Cell Events: 2253 mean_circ: 0.940048
Ran for 66550 frames Cell Events: 2277 mean_circ: 0.939942
Ran for 67050 frames Cell Events: 2300 mean_circ: 0.939862
Ran for 68050 frames Cell Events: 2337 mean_circ: 0.939961
Ran for 68550 frames Cell Events: 2361 mean_circ: 0.939958
Ran for 69050 frames Cell Events: 2384 mean_circ: 0.939984
Ran for 70050 frames Cell Events: 2428 mean_circ: 0.939793
Ran for 70550 frames Cell Events: 2448 mean_circ: 0.939654
Ran for 71050 frames Cell Events: 2464 mean_circ: 0.939498
Ran for 71550 frames Cell Events: 2476 mean_circ: 0.939505
Ran for 72050 frames Cell Events: 2496 mean_circ: 0.939532
Ran for 72550 frames Cell Events: 2512 mean_circ: 0.939553
Ran for 73050 frames Cell Events: 2525 mean_circ: 0.939556
Ran for 74050 frames Cell Events: 2576 mean_circ: 0.939598
Ran for 76050 frames Cell Events: 2681 mean_circ: 0.939312
Ran for 76550 frames Cell Events: 2700 mean_circ: 0.939335
Ran for 77050 frames Cell Events: 2715 mean_circ: 0.939354
Ran for 78050 frames Cell Events: 2761 mean_circ: 0.939334
Ran for 79550 frames Cell Events: 2808 mean_circ: 0.939433
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Ran for 82050 frames Cell Events: 2910 mean_circ: 0.939344
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Ran for 83050 frames Cell Events: 2958 mean_circ: 0.939332
Ran for 84050 frames Cell Events: 2998 mean_circ: 0.939306
Ran for 84550 frames Cell Events: 3017 mean_circ: 0.939263
Ran for 85050 frames Cell Events: 3042 mean_circ: 0.939308
Ran for 85550 frames Cell Events: 3062 mean_circ: 0.939276
Ran for 86050 frames Cell Events: 3081 mean_circ: 0.939202
Ran for 87050 frames Cell Events: 3125 mean_circ: 0.939286
Ran for 87550 frames Cell Events: 3154 mean_circ: 0.939325
Ran for 89050 frames Cell Events: 3236 mean_circ: 0.938996
Ran for 90550 frames Cell Events: 3294 mean_circ: 0.939089
Ran for 92550 frames Cell Events: 3406 mean_circ: 0.939117
Ran for 93050 frames Cell Events: 3425 mean_circ: 0.939131
Ran for 93550 frames Cell Events: 3445 mean_circ: 0.939202
Ran for 94050 frames Cell Events: 3462 mean_circ: 0.939229
Ran for 94550 frames Cell Events: 3474 mean_circ: 0.939183
Ran for 95550 frames Cell Events: 3516 mean_circ: 0.9393
Ran for 96550 frames Cell Events: 3558 mean_circ: 0.939264
Ran for 97050 frames Cell Events: 3583 mean_circ: 0.939173
Ran for 97550 frames Cell Events: 3603 mean_circ: 0.939154
Ran for 98050 frames Cell Events: 3621 mean_circ: 0.939229
Ran for 98550 frames Cell Events: 3643 mean_circ: 0.939174
Ran for 99050 frames Cell Events: 3680 mean_circ: 0.939053
Ran for 100050 frames Cell Events: 3736 mean_circ: 0.939031
Ran for 100550 frames Cell Events: 3757 mean_circ: 0.93903
Ran for 101050 frames Cell Events: 3781 mean_circ: 0.939005
Ran for 101550 frames Cell Events: 3799 mean_circ: 0.938975
Ran for 102050 frames Cell Events: 3821 mean_circ: 0.938986
Ran for 102550 frames Cell Events: 3842 mean_circ: 0.93902
Ran for 103050 frames Cell Events: 3862 mean_circ: 0.938982
Ran for 103550 frames Cell Events: 3880 mean_circ: 0.939026
Ran for 104050 frames Cell Events: 3908 mean_circ: 0.938996
Ran for 105050 frames Cell Events: 3956 mean_circ: 0.938853
Ran for 105550 frames Cell Events: 3977 mean_circ: 0.938811
Ran for 107050 frames Cell Events: 4047 mean_circ: 0.938717
Ran for 108550 frames Cell Events: 4108 mean_circ: 0.938709
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Ran for 111050 frames Cell Events: 4203 mean_circ: 0.938647
Ran for 111550 frames Cell Events: 4224 mean_circ: 0.938662
Ran for 113050 frames Cell Events: 4268 mean_circ: 0.938699
Ran for 113550 frames Cell Events: 4284 mean_circ: 0.938701
Ran for 114550 frames Cell Events: 4325 mean_circ: 0.938549
Ran for 115050 frames Cell Events: 4339 mean_circ: 0.938587
Ran for 115550 frames Cell Events: 4367 mean_circ: 0.938551
Ran for 116550 frames Cell Events: 4410 mean_circ: 0.938578
Ran for 117550 frames Cell Events: 4448 mean_circ: 0.938687
Ran for 118050 frames Cell Events: 4466 mean_circ: 0.93872
Ran for 118550 frames Cell Events: 4485 mean_circ: 0.938727
Ran for 120050 frames Cell Events: 4543 mean_circ: 0.938697
Ran for 120550 frames Cell Events: 4559 mean_circ: 0.938706
Ran for 122050 frames Cell Events: 4598 mean_circ: 0.938637
Ran for 123050 frames Cell Events: 4650 mean_circ: 0.938702
Ran for 124050 frames Cell Events: 4693 mean_circ: 0.938692
Ran for 124550 frames Cell Events: 4713 mean_circ: 0.938729
Ran for 126550 frames Cell Events: 4805 mean_circ: 0.938788
Ran for 127050 frames Cell Events: 4835 mean_circ: 0.938859
Ran for 127550 frames Cell Events: 4844 mean_circ: 0.938911
Ran for 128050 frames Cell Events: 4864 mean_circ: 0.93886
Ran for 129550 frames Cell Events: 4932 mean_circ: 0.938749
Ran for 130050 frames Cell Events: 4953 mean_circ: 0.938765
Ran for 131050 frames Cell Events: 4996 mean_circ: 0.938709
Ran for 131550 frames Cell Events: 5028 mean_circ: 0.938659
Ran for 132550 frames Cell Events: 5068 mean_circ: 0.938668
Ran for 133050 frames Cell Events: 5091 mean_circ: 0.938653
Ran for 133550 frames Cell Events: 5104 mean_circ: 0.938645
Ran for 134050 frames Cell Events: 5125 mean_circ: 0.938674
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Ran for 136050 frames Cell Events: 5194 mean_circ: 0.938631
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Ran for 137050 frames Cell Events: 5242 mean_circ: 0.938468
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Ran for 143050 frames Cell Events: 5482 mean_circ: 0.938503
Ran for 143550 frames Cell Events: 5503 mean_circ: 0.938521
Ran for 144050 frames Cell Events: 5516 mean_circ: 0.938537
Ran for 144550 frames Cell Events: 5538 mean_circ: 0.938428
Ran for 146050 frames Cell Events: 5621 mean_circ: 0.938493
Ran for 147050 frames Cell Events: 5655 mean_circ: 0.938574
Ran for 147550 frames Cell Events: 5673 mean_circ: 0.938563
Ran for 148050 frames Cell Events: 5686 mean_circ: 0.938574
Ran for 149050 frames Cell Events: 5719 mean_circ: 0.938566
Ran for 150050 frames Cell Events: 5762 mean_circ: 0.938504
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Ran for 151050 frames Cell Events: 5793 mean_circ: 0.938507
Ran for 151550 frames Cell Events: 5812 mean_circ: 0.93849
Ran for 152550 frames Cell Events: 5855 mean_circ: 0.93852
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Ran for 157050 frames Cell Events: 6046 mean_circ: 0.938642
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Ran for 171550 frames Cell Events: 6596 mean_circ: 0.938673
Ran for 173050 frames Cell Events: 6656 mean_circ: 0.938674
Ran for 174050 frames Cell Events: 6703 mean_circ: 0.938635
Ran for 174550 frames Cell Events: 6714 mean_circ: 0.93864
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Ran for 175550 frames Cell Events: 6754 mean_circ: 0.938653
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Ran for 178050 frames Cell Events: 6851 mean_circ: 0.93873
Ran for 178550 frames Cell Events: 6870 mean_circ: 0.938751
Ran for 180050 frames Cell Events: 6929 mean_circ: 0.938793
Ran for 180550 frames Cell Events: 6950 mean_circ: 0.938786
Ran for 181550 frames Cell Events: 6995 mean_circ: 0.938787
Ran for 182050 frames Cell Events: 7020 mean_circ: 0.938751
Ran for 182550 frames Cell Events: 7039 mean_circ: 0.93871
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Ran for 183550 frames Cell Events: 7085 mean_circ: 0.938769
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Ran for 192050 frames Cell Events: 7453 mean_circ: 0.938584
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Ran for 195550 frames Cell Events: 7575 mean_circ: 0.938563
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Ran for 198050 frames Cell Events: 7687 mean_circ: 0.938505
Ran for 198550 frames Cell Events: 7710 mean_circ: 0.938437
Ran for 199050 frames Cell Events: 7731 mean_circ: 0.938433
Ran for 199550 frames Cell Events: 7747 mean_circ: 0.938425
Ran for 200050 frames Cell Events: 7773 mean_circ: 0.938482
Ran for 201050 frames Cell Events: 7805 mean_circ: 0.938461
Ran for 202050 frames Cell Events: 7846 mean_circ: 0.938427
Ran for 203550 frames Cell Events: 7906 mean_circ: 0.938428
Ran for 204050 frames Cell Events: 7926 mean_circ: 0.938436
Ran for 204550 frames Cell Events: 7939 mean_circ: 0.93845
Ran for 205050 frames Cell Events: 7959 mean_circ: 0.938496
Ran for 205550 frames Cell Events: 7983 mean_circ: 0.938489
Ran for 206050 frames Cell Events: 8012 mean_circ: 0.938504
Ran for 207550 frames Cell Events: 8089 mean_circ: 0.938453
Ran for 208550 frames Cell Events: 8131 mean_circ: 0.938413
Ran for 209050 frames Cell Events: 8146 mean_circ: 0.938387
Ran for 209550 frames Cell Events: 8159 mean_circ: 0.938391
Ran for 210550 frames Cell Events: 8197 mean_circ: 0.93838
Ran for 211050 frames Cell Events: 8219 mean_circ: 0.938393
Ran for 212050 frames Cell Events: 8255 mean_circ: 0.93842
Ran for 212550 frames Cell Events: 8278 mean_circ: 0.938413
Ran for 213050 frames Cell Events: 8297 mean_circ: 0.938414



End this measurment..



Stopping time: 2.0962e+009
****************************************
Measurement finished

Mean Circularitiy: 0.938424
Mean Area: 636.494
Mean FL-1 intensity: 3944.98
Mean FL-2 intensity: 5242.53
Mean FL-3 intensity: 0

frames processed: 211733
Total Time: 106803ms
Number of Runs (new bg img) 0
Frames collected: 213485
Skipped frames: 26100
Rescued Frames: 1666760
Samples DAQ: 53371396
Samples written: 106742188
Samples per Channel: 53371094
FPS: 1998.87

Samples per second: 499717
Expected samples/s: 500000

Events: 8311
Events per second: 77.8164
jumped back (PMT): 12
#Frames read, frequency
# 0 frames :306115
# 1 frames :104492
# 2 frames :4528
# 3 frames :1486
# 4 frames :804
# 5 frames :410
# 6 frames :2379
# 7 frames :1822
# 8 frames :1100
# 9 frames :601
# 10 frames :578
# 11 frames :399
# 12 frames :404
# 13 frames :213
# 14 frames :240
# 15 frames :218
# 16 frames :178
# 17 frames :174
# 18 frames :166
# 19 frames :141
# 20 frames :111
# 21 frames :68
# 22 frames :70
# 23 frames :38
# 24 frames :41
# 25 frames :22
# 26 frames :25
# 27 frames :14
# 28 frames :9
# 29 frames :10
# 30 frames :6
# 31 frames :9
# 32 frames :4
# 33 frames :7
# 34 frames :8
# 35 frames :5
# 36 frames :1
# 37 frames :5
# 39 frames :5
# 40 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :1
# 46 frames :2
# 48 frames :1
# 49 frames :1
# 55 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6216
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0