Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.42.33 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "f48555fb9ad20b71f5c2837751a7d292",
"name": "M1_20170608_2026_dsRNA_R7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:42:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8044 (max 8045) in '8044'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1595
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.117e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.947493
Ran for 1050 frames Cell Events: 48 mean_circ: 0.940291
Ran for 1550 frames Cell Events: 70 mean_circ: 0.938921
Ran for 2050 frames Cell Events: 94 mean_circ: 0.936127
Ran for 3050 frames Cell Events: 127 mean_circ: 0.939268
Ran for 3550 frames Cell Events: 150 mean_circ: 0.938545
Ran for 4550 frames Cell Events: 185 mean_circ: 0.939187
Ran for 6050 frames Cell Events: 245 mean_circ: 0.939242
Ran for 7050 frames Cell Events: 262 mean_circ: 0.939724
Ran for 7550 frames Cell Events: 286 mean_circ: 0.938264
Ran for 8050 frames Cell Events: 310 mean_circ: 0.938274
Ran for 9050 frames Cell Events: 343 mean_circ: 0.937777
Ran for 9550 frames Cell Events: 365 mean_circ: 0.938007
Ran for 10050 frames Cell Events: 402 mean_circ: 0.937435
Ran for 10550 frames Cell Events: 427 mean_circ: 0.936832
Ran for 11050 frames Cell Events: 439 mean_circ: 0.936768
Ran for 13050 frames Cell Events: 506 mean_circ: 0.937335
Ran for 14550 frames Cell Events: 555 mean_circ: 0.937387
Ran for 15050 frames Cell Events: 571 mean_circ: 0.937571
Ran for 16050 frames Cell Events: 610 mean_circ: 0.937978
Ran for 16550 frames Cell Events: 630 mean_circ: 0.938114
Ran for 17050 frames Cell Events: 658 mean_circ: 0.93745
Ran for 17550 frames Cell Events: 676 mean_circ: 0.937432
Ran for 18050 frames Cell Events: 705 mean_circ: 0.937647
Ran for 18550 frames Cell Events: 735 mean_circ: 0.937733
Ran for 20050 frames Cell Events: 838 mean_circ: 0.937313
Ran for 20550 frames Cell Events: 857 mean_circ: 0.937402
Ran for 21050 frames Cell Events: 885 mean_circ: 0.937402
Ran for 22550 frames Cell Events: 939 mean_circ: 0.937004
Ran for 23050 frames Cell Events: 959 mean_circ: 0.937258
Ran for 23550 frames Cell Events: 976 mean_circ: 0.937055
Ran for 24050 frames Cell Events: 1003 mean_circ: 0.936981
Ran for 24550 frames Cell Events: 1036 mean_circ: 0.937274
Ran for 25050 frames Cell Events: 1051 mean_circ: 0.937106
Ran for 25550 frames Cell Events: 1074 mean_circ: 0.937204
Ran for 26550 frames Cell Events: 1108 mean_circ: 0.937383
Ran for 27050 frames Cell Events: 1129 mean_circ: 0.937392
Ran for 27550 frames Cell Events: 1147 mean_circ: 0.937285
Ran for 28050 frames Cell Events: 1172 mean_circ: 0.937036
Ran for 29050 frames Cell Events: 1221 mean_circ: 0.936685
Ran for 29550 frames Cell Events: 1240 mean_circ: 0.936774
Ran for 30050 frames Cell Events: 1253 mean_circ: 0.936829
Ran for 30550 frames Cell Events: 1276 mean_circ: 0.936455
Ran for 31050 frames Cell Events: 1300 mean_circ: 0.93668
Ran for 31550 frames Cell Events: 1325 mean_circ: 0.936299
Ran for 32050 frames Cell Events: 1343 mean_circ: 0.936181
Ran for 32550 frames Cell Events: 1357 mean_circ: 0.936254
Ran for 33550 frames Cell Events: 1395 mean_circ: 0.936211
Ran for 34050 frames Cell Events: 1405 mean_circ: 0.936312
Ran for 35050 frames Cell Events: 1450 mean_circ: 0.936101
Ran for 35550 frames Cell Events: 1462 mean_circ: 0.936079
Ran for 36050 frames Cell Events: 1487 mean_circ: 0.93601
Ran for 37550 frames Cell Events: 1555 mean_circ: 0.936288
Ran for 38050 frames Cell Events: 1576 mean_circ: 0.936467
Ran for 39050 frames Cell Events: 1606 mean_circ: 0.936529
Ran for 39550 frames Cell Events: 1632 mean_circ: 0.936668
Ran for 40050 frames Cell Events: 1659 mean_circ: 0.936679
Ran for 40550 frames Cell Events: 1677 mean_circ: 0.936759
Ran for 41050 frames Cell Events: 1700 mean_circ: 0.936811
Ran for 41550 frames Cell Events: 1727 mean_circ: 0.936885
Ran for 42050 frames Cell Events: 1749 mean_circ: 0.936889
Ran for 42550 frames Cell Events: 1761 mean_circ: 0.936952
Ran for 43050 frames Cell Events: 1777 mean_circ: 0.936866
Ran for 43550 frames Cell Events: 1797 mean_circ: 0.936904
Ran for 44550 frames Cell Events: 1838 mean_circ: 0.936841
Ran for 45050 frames Cell Events: 1858 mean_circ: 0.936933
Ran for 45550 frames Cell Events: 1870 mean_circ: 0.937012
Ran for 46050 frames Cell Events: 1894 mean_circ: 0.936984
Ran for 47050 frames Cell Events: 1943 mean_circ: 0.937054
Ran for 47550 frames Cell Events: 1968 mean_circ: 0.937049
Ran for 48550 frames Cell Events: 1998 mean_circ: 0.937063
Ran for 49050 frames Cell Events: 2020 mean_circ: 0.937081
Ran for 49550 frames Cell Events: 2041 mean_circ: 0.93703
Ran for 50050 frames Cell Events: 2068 mean_circ: 0.937015
Ran for 50550 frames Cell Events: 2080 mean_circ: 0.937012
Ran for 51050 frames Cell Events: 2093 mean_circ: 0.936947
Ran for 51550 frames Cell Events: 2111 mean_circ: 0.937047
Ran for 52050 frames Cell Events: 2136 mean_circ: 0.937047
Ran for 52550 frames Cell Events: 2154 mean_circ: 0.936803
Ran for 53050 frames Cell Events: 2175 mean_circ: 0.936821
Ran for 53550 frames Cell Events: 2197 mean_circ: 0.936748
Ran for 54550 frames Cell Events: 2250 mean_circ: 0.936766
Ran for 55050 frames Cell Events: 2270 mean_circ: 0.936675
Ran for 56050 frames Cell Events: 2322 mean_circ: 0.936798
Ran for 56550 frames Cell Events: 2345 mean_circ: 0.936603
Ran for 57050 frames Cell Events: 2364 mean_circ: 0.936615
Ran for 58050 frames Cell Events: 2409 mean_circ: 0.936666
Ran for 58550 frames Cell Events: 2426 mean_circ: 0.936698
Ran for 59050 frames Cell Events: 2459 mean_circ: 0.936656
Ran for 60050 frames Cell Events: 2504 mean_circ: 0.936748
Ran for 60550 frames Cell Events: 2532 mean_circ: 0.936878
Ran for 61050 frames Cell Events: 2552 mean_circ: 0.93693
Ran for 62050 frames Cell Events: 2605 mean_circ: 0.936946
Ran for 62550 frames Cell Events: 2621 mean_circ: 0.936998
Ran for 63050 frames Cell Events: 2636 mean_circ: 0.936985
Ran for 64550 frames Cell Events: 2687 mean_circ: 0.937028
Ran for 65050 frames Cell Events: 2701 mean_circ: 0.937108
Ran for 65550 frames Cell Events: 2722 mean_circ: 0.937094
Ran for 66050 frames Cell Events: 2740 mean_circ: 0.937131
Ran for 66550 frames Cell Events: 2764 mean_circ: 0.937139
Ran for 67050 frames Cell Events: 2782 mean_circ: 0.937062
Ran for 67550 frames Cell Events: 2810 mean_circ: 0.936991
Ran for 69050 frames Cell Events: 2894 mean_circ: 0.936719
Ran for 69550 frames Cell Events: 2914 mean_circ: 0.936769
Ran for 71050 frames Cell Events: 2970 mean_circ: 0.936874
Ran for 74550 frames Cell Events: 3185 mean_circ: 0.93693
Ran for 75550 frames Cell Events: 3224 mean_circ: 0.936855
Ran for 76550 frames Cell Events: 3265 mean_circ: 0.936767
Ran for 77050 frames Cell Events: 3283 mean_circ: 0.936792
Ran for 78550 frames Cell Events: 3383 mean_circ: 0.936781
Ran for 81550 frames Cell Events: 3506 mean_circ: 0.936642
Ran for 82550 frames Cell Events: 3542 mean_circ: 0.936719
Ran for 83050 frames Cell Events: 3561 mean_circ: 0.93666
Ran for 83550 frames Cell Events: 3583 mean_circ: 0.936645
Ran for 84550 frames Cell Events: 3628 mean_circ: 0.936556
Ran for 86050 frames Cell Events: 3702 mean_circ: 0.936402
Ran for 86550 frames Cell Events: 3719 mean_circ: 0.936405
Ran for 87050 frames Cell Events: 3743 mean_circ: 0.936411
Ran for 88550 frames Cell Events: 3807 mean_circ: 0.936371
Ran for 90050 frames Cell Events: 3878 mean_circ: 0.936385
Ran for 90550 frames Cell Events: 3900 mean_circ: 0.936429
Ran for 91050 frames Cell Events: 3919 mean_circ: 0.936333
Ran for 91550 frames Cell Events: 3949 mean_circ: 0.936399
Ran for 92550 frames Cell Events: 4000 mean_circ: 0.93638
Ran for 93050 frames Cell Events: 4023 mean_circ: 0.936371
Ran for 93550 frames Cell Events: 4047 mean_circ: 0.936388
Ran for 95050 frames Cell Events: 4097 mean_circ: 0.936346
Ran for 97050 frames Cell Events: 4179 mean_circ: 0.936385
Ran for 98550 frames Cell Events: 4249 mean_circ: 0.936226
Ran for 100050 frames Cell Events: 4312 mean_circ: 0.936222
Ran for 101550 frames Cell Events: 4399 mean_circ: 0.936092
Ran for 102050 frames Cell Events: 4422 mean_circ: 0.93615
Ran for 103550 frames Cell Events: 4469 mean_circ: 0.936066
Ran for 104550 frames Cell Events: 4519 mean_circ: 0.936105
Ran for 106050 frames Cell Events: 4560 mean_circ: 0.936147
Ran for 107550 frames Cell Events: 4623 mean_circ: 0.935938
Ran for 109050 frames Cell Events: 4690 mean_circ: 0.935892
Ran for 109550 frames Cell Events: 4715 mean_circ: 0.935802
Ran for 110050 frames Cell Events: 4737 mean_circ: 0.935755
Ran for 111050 frames Cell Events: 4796 mean_circ: 0.935714
Ran for 112050 frames Cell Events: 4830 mean_circ: 0.935779
Ran for 113050 frames Cell Events: 4875 mean_circ: 0.935852
Ran for 113550 frames Cell Events: 4899 mean_circ: 0.935851
Ran for 115050 frames Cell Events: 4972 mean_circ: 0.935823
Ran for 116550 frames Cell Events: 5022 mean_circ: 0.935778
Ran for 117050 frames Cell Events: 5035 mean_circ: 0.935777
Ran for 117550 frames Cell Events: 5053 mean_circ: 0.935819
Ran for 118050 frames Cell Events: 5076 mean_circ: 0.935821
Ran for 119050 frames Cell Events: 5124 mean_circ: 0.935833
Ran for 121050 frames Cell Events: 5206 mean_circ: 0.935794
Ran for 121550 frames Cell Events: 5221 mean_circ: 0.93585
Ran for 123550 frames Cell Events: 5307 mean_circ: 0.935874
Ran for 125050 frames Cell Events: 5384 mean_circ: 0.935843
Ran for 127550 frames Cell Events: 5494 mean_circ: 0.935825
Ran for 128050 frames Cell Events: 5516 mean_circ: 0.935792
Ran for 130050 frames Cell Events: 5610 mean_circ: 0.935795
Ran for 130550 frames Cell Events: 5631 mean_circ: 0.935835
Ran for 131050 frames Cell Events: 5646 mean_circ: 0.93576
Ran for 131550 frames Cell Events: 5667 mean_circ: 0.935763
Ran for 132550 frames Cell Events: 5727 mean_circ: 0.935755
Ran for 133050 frames Cell Events: 5751 mean_circ: 0.935787
Ran for 134550 frames Cell Events: 5819 mean_circ: 0.935793
Ran for 135050 frames Cell Events: 5845 mean_circ: 0.935797
Ran for 135550 frames Cell Events: 5868 mean_circ: 0.935773
Ran for 136050 frames Cell Events: 5886 mean_circ: 0.935774
Ran for 136550 frames Cell Events: 5902 mean_circ: 0.935751
Ran for 137050 frames Cell Events: 5921 mean_circ: 0.935751
Ran for 139050 frames Cell Events: 5996 mean_circ: 0.935765
Ran for 141050 frames Cell Events: 6076 mean_circ: 0.935747
Ran for 142050 frames Cell Events: 6136 mean_circ: 0.935728
Ran for 143050 frames Cell Events: 6187 mean_circ: 0.935657
Ran for 143550 frames Cell Events: 6204 mean_circ: 0.935689
Ran for 144050 frames Cell Events: 6230 mean_circ: 0.935611
Ran for 144550 frames Cell Events: 6253 mean_circ: 0.935579
Ran for 145050 frames Cell Events: 6276 mean_circ: 0.935578
Ran for 146050 frames Cell Events: 6310 mean_circ: 0.935547
Ran for 146550 frames Cell Events: 6335 mean_circ: 0.935527
Ran for 147050 frames Cell Events: 6356 mean_circ: 0.935548
Ran for 147550 frames Cell Events: 6374 mean_circ: 0.935528
Ran for 148550 frames Cell Events: 6414 mean_circ: 0.935548
Ran for 149050 frames Cell Events: 6437 mean_circ: 0.935571
Ran for 149550 frames Cell Events: 6453 mean_circ: 0.935586
Ran for 150050 frames Cell Events: 6475 mean_circ: 0.935607
Ran for 153550 frames Cell Events: 6605 mean_circ: 0.935544
Ran for 154050 frames Cell Events: 6628 mean_circ: 0.935557
Ran for 154550 frames Cell Events: 6654 mean_circ: 0.935521
Ran for 155050 frames Cell Events: 6669 mean_circ: 0.935532
Ran for 155550 frames Cell Events: 6691 mean_circ: 0.935552
Ran for 156550 frames Cell Events: 6733 mean_circ: 0.935609
Ran for 157550 frames Cell Events: 6765 mean_circ: 0.935587
Ran for 159550 frames Cell Events: 6851 mean_circ: 0.935598
Ran for 160050 frames Cell Events: 6871 mean_circ: 0.935552
Ran for 160550 frames Cell Events: 6899 mean_circ: 0.935587
Ran for 161050 frames Cell Events: 6914 mean_circ: 0.935556
Ran for 162550 frames Cell Events: 6978 mean_circ: 0.93551
Ran for 163050 frames Cell Events: 6999 mean_circ: 0.935523
Ran for 164050 frames Cell Events: 7054 mean_circ: 0.935506
Ran for 165050 frames Cell Events: 7103 mean_circ: 0.935461
Ran for 165550 frames Cell Events: 7127 mean_circ: 0.935459
Ran for 167050 frames Cell Events: 7192 mean_circ: 0.935427
Ran for 169050 frames Cell Events: 7265 mean_circ: 0.935452
Ran for 169550 frames Cell Events: 7282 mean_circ: 0.935411
Ran for 170050 frames Cell Events: 7295 mean_circ: 0.935454
Ran for 171050 frames Cell Events: 7329 mean_circ: 0.93542
Ran for 171550 frames Cell Events: 7352 mean_circ: 0.935406
Ran for 172050 frames Cell Events: 7379 mean_circ: 0.935431
Ran for 172550 frames Cell Events: 7405 mean_circ: 0.935438
Ran for 173050 frames Cell Events: 7427 mean_circ: 0.935454
Ran for 174050 frames Cell Events: 7447 mean_circ: 0.935414
Ran for 174550 frames Cell Events: 7475 mean_circ: 0.935396
Ran for 175050 frames Cell Events: 7506 mean_circ: 0.9354
Ran for 176050 frames Cell Events: 7539 mean_circ: 0.935298
Ran for 177050 frames Cell Events: 7581 mean_circ: 0.935291
Ran for 177550 frames Cell Events: 7601 mean_circ: 0.935319
Ran for 178050 frames Cell Events: 7620 mean_circ: 0.935321
Ran for 180050 frames Cell Events: 7731 mean_circ: 0.935313
Ran for 181550 frames Cell Events: 7788 mean_circ: 0.935325
Ran for 184050 frames Cell Events: 7895 mean_circ: 0.935288
Ran for 184550 frames Cell Events: 7919 mean_circ: 0.93528
Ran for 185050 frames Cell Events: 7941 mean_circ: 0.935238



End this measurment..



Stopping time: 2.1171e+009
****************************************
Measurement finished

Mean Circularitiy: 0.935218
Mean Area: 646.723
Mean FL-1 intensity: 3761.68
Mean FL-2 intensity: 4800.25
Mean FL-3 intensity: 0

frames processed: 183828
Total Time: 93635.1ms
Number of Runs (new bg img) 0
Frames collected: 187160
Skipped frames: 35909
Rescued Frames: 3224988
Samples DAQ: 46790839
Samples written: 93579752
Samples per Channel: 46789876
FPS: 1998.82

Samples per second: 499705
Expected samples/s: 500000

Events: 8045
Events per second: 85.9187
jumped back (PMT): 26
#Frames read, frequency
# 0 frames :279934
# 1 frames :79364
# 2 frames :3769
# 3 frames :1257
# 4 frames :836
# 5 frames :416
# 6 frames :1464
# 7 frames :1579
# 8 frames :1241
# 9 frames :758
# 10 frames :542
# 11 frames :435
# 12 frames :398
# 13 frames :311
# 14 frames :257
# 15 frames :201
# 16 frames :167
# 17 frames :143
# 18 frames :115
# 19 frames :104
# 20 frames :87
# 21 frames :69
# 22 frames :69
# 23 frames :55
# 24 frames :42
# 25 frames :43
# 26 frames :30
# 27 frames :27
# 28 frames :25
# 29 frames :25
# 30 frames :21
# 31 frames :12
# 32 frames :17
# 33 frames :19
# 34 frames :10
# 35 frames :8
# 36 frames :12
# 37 frames :4
# 38 frames :7
# 39 frames :8
# 40 frames :2
# 41 frames :4
# 42 frames :3
# 43 frames :6
# 44 frames :4
# 45 frames :4
# 46 frames :2
# 47 frames :2
# 49 frames :2
# 50 frames :3
# 51 frames :2
# 52 frames :1
# 53 frames :2
# 54 frames :1
# 56 frames :2
# 59 frames :1
# 60 frames :1
# 61 frames :3
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1595
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0