Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.55.18 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "29e5f27e71383b9083d3c184ec329a2e",
"name": "M1_20170609_1230_dsRNA_X2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:55:41"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8611 (max 8612) in '8611'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0274
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.17487e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.952366
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Ran for 2550 frames Cell Events: 79 mean_circ: 0.941316
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Ran for 7050 frames Cell Events: 246 mean_circ: 0.943935
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Ran for 10050 frames Cell Events: 339 mean_circ: 0.942654
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Ran for 74550 frames Cell Events: 2465 mean_circ: 0.942693
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Ran for 77550 frames Cell Events: 2548 mean_circ: 0.942365
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Ran for 92050 frames Cell Events: 3053 mean_circ: 0.942253
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Ran for 93550 frames Cell Events: 3097 mean_circ: 0.942229
Ran for 94050 frames Cell Events: 3112 mean_circ: 0.942254
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Ran for 96550 frames Cell Events: 3201 mean_circ: 0.942405
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Ran for 100550 frames Cell Events: 3327 mean_circ: 0.942573
Ran for 101050 frames Cell Events: 3351 mean_circ: 0.942602
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Ran for 102550 frames Cell Events: 3406 mean_circ: 0.942585
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Ran for 105050 frames Cell Events: 3491 mean_circ: 0.942577
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Ran for 106550 frames Cell Events: 3548 mean_circ: 0.94239
Ran for 107550 frames Cell Events: 3596 mean_circ: 0.942478
Ran for 108550 frames Cell Events: 3628 mean_circ: 0.942486
Ran for 109050 frames Cell Events: 3647 mean_circ: 0.942537
Ran for 110550 frames Cell Events: 3713 mean_circ: 0.942483
Ran for 112050 frames Cell Events: 3764 mean_circ: 0.942445
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Ran for 126550 frames Cell Events: 4226 mean_circ: 0.942106
Ran for 127050 frames Cell Events: 4252 mean_circ: 0.942092
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Ran for 133050 frames Cell Events: 4463 mean_circ: 0.941984
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Ran for 142550 frames Cell Events: 4763 mean_circ: 0.942077
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Ran for 216550 frames Cell Events: 7367 mean_circ: 0.941398
Ran for 217050 frames Cell Events: 7382 mean_circ: 0.941413
Ran for 217550 frames Cell Events: 7401 mean_circ: 0.941342
Ran for 219050 frames Cell Events: 7469 mean_circ: 0.941216
Ran for 220050 frames Cell Events: 7507 mean_circ: 0.941171
Ran for 220550 frames Cell Events: 7523 mean_circ: 0.941172
Ran for 221550 frames Cell Events: 7565 mean_circ: 0.941176
Ran for 222050 frames Cell Events: 7579 mean_circ: 0.941174
Ran for 222550 frames Cell Events: 7596 mean_circ: 0.94117
Ran for 223550 frames Cell Events: 7639 mean_circ: 0.941112
Ran for 224050 frames Cell Events: 7650 mean_circ: 0.941121
Ran for 224550 frames Cell Events: 7664 mean_circ: 0.941075
Ran for 225050 frames Cell Events: 7678 mean_circ: 0.941062
Ran for 225550 frames Cell Events: 7693 mean_circ: 0.941082
Ran for 226550 frames Cell Events: 7722 mean_circ: 0.941044
Ran for 227050 frames Cell Events: 7740 mean_circ: 0.941034
Ran for 228050 frames Cell Events: 7764 mean_circ: 0.941071
Ran for 228550 frames Cell Events: 7780 mean_circ: 0.941061
Ran for 229050 frames Cell Events: 7794 mean_circ: 0.941064
Ran for 229550 frames Cell Events: 7816 mean_circ: 0.941055
Ran for 230050 frames Cell Events: 7834 mean_circ: 0.941018
Ran for 233050 frames Cell Events: 7929 mean_circ: 0.941027
Ran for 233550 frames Cell Events: 7950 mean_circ: 0.941015
Ran for 234050 frames Cell Events: 7971 mean_circ: 0.941043
Ran for 234550 frames Cell Events: 7999 mean_circ: 0.941025
Ran for 235050 frames Cell Events: 8017 mean_circ: 0.941052
Ran for 235550 frames Cell Events: 8025 mean_circ: 0.941003
Ran for 236050 frames Cell Events: 8036 mean_circ: 0.941008
Ran for 237550 frames Cell Events: 8089 mean_circ: 0.940956
Ran for 238550 frames Cell Events: 8128 mean_circ: 0.940973
Ran for 239550 frames Cell Events: 8167 mean_circ: 0.940984
Ran for 240050 frames Cell Events: 8181 mean_circ: 0.941014
Ran for 241050 frames Cell Events: 8212 mean_circ: 0.941005
Ran for 241550 frames Cell Events: 8234 mean_circ: 0.941016
Ran for 243050 frames Cell Events: 8292 mean_circ: 0.941065
Ran for 243550 frames Cell Events: 8307 mean_circ: 0.941044
Ran for 244550 frames Cell Events: 8353 mean_circ: 0.940973
Ran for 246050 frames Cell Events: 8407 mean_circ: 0.940945
Ran for 247550 frames Cell Events: 8448 mean_circ: 0.94088
Ran for 248050 frames Cell Events: 8471 mean_circ: 0.940877
Ran for 248550 frames Cell Events: 8488 mean_circ: 0.940882
Ran for 249050 frames Cell Events: 8507 mean_circ: 0.940877
Ran for 249550 frames Cell Events: 8527 mean_circ: 0.940883
Ran for 251050 frames Cell Events: 8586 mean_circ: 0.940878
Ran for 251550 frames Cell Events: 8599 mean_circ: 0.940886
Ran for 252050 frames Cell Events: 8608 mean_circ: 0.94088



End this measurment..



Stopping time: 2.175e+009
****************************************
Measurement finished

Mean Circularitiy: 0.940882
Mean Area: 614.109
Mean FL-1 intensity: 3369.22
Mean FL-2 intensity: 4588.99
Mean FL-3 intensity: 0

frames processed: 251047
Total Time: 126136ms
Number of Runs (new bg img) 0
Frames collected: 252154
Skipped frames: 7102
Rescued Frames: 721044
Samples DAQ: 63038451
Samples written: 126076780
Samples per Channel: 63038390
FPS: 1999.07

Samples per second: 499766
Expected samples/s: 500000

Events: 8612
Events per second: 68.2757
jumped back (PMT): 32
#Frames read, frequency
# 0 frames :373253
# 1 frames :112020
# 2 frames :4806
# 3 frames :2135
# 4 frames :1297
# 5 frames :707
# 6 frames :666
# 7 frames :1832
# 8 frames :1259
# 9 frames :971
# 10 frames :986
# 11 frames :873
# 12 frames :689
# 13 frames :508
# 14 frames :357
# 15 frames :264
# 16 frames :224
# 17 frames :208
# 18 frames :207
# 19 frames :182
# 20 frames :143
# 21 frames :112
# 22 frames :94
# 23 frames :67
# 24 frames :72
# 25 frames :43
# 26 frames :43
# 27 frames :34
# 28 frames :22
# 29 frames :29
# 30 frames :21
# 31 frames :26
# 32 frames :13
# 33 frames :13
# 34 frames :8
# 35 frames :8
# 36 frames :8
# 37 frames :12
# 38 frames :12
# 39 frames :3
# 40 frames :5
# 41 frames :3
# 42 frames :9
# 43 frames :4
# 44 frames :1
# 45 frames :7
# 46 frames :2
# 47 frames :4
# 48 frames :3
# 49 frames :2
# 50 frames :1
# 51 frames :2
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 56 frames :2
# 58 frames :1
# 59 frames :1
# 61 frames :1
# 62 frames :1
# 66 frames :2
# 68 frames :2
# 72 frames :1
# 99 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0274
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0