Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_15.20.17 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a629111d2a67865fda9a0784542fbff0",
"name": "M1_20170609_1314_dsRNA_X5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:20:39"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9447 (max 9448) in '9447'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.189
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.17746e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 17 mean_circ: 0.942334
Ran for 1050 frames Cell Events: 28 mean_circ: 0.939246
Ran for 1550 frames Cell Events: 43 mean_circ: 0.939363
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Ran for 6550 frames Cell Events: 189 mean_circ: 0.94309
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Ran for 9550 frames Cell Events: 281 mean_circ: 0.943074
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Ran for 79550 frames Cell Events: 2794 mean_circ: 0.941905
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Ran for 81050 frames Cell Events: 2842 mean_circ: 0.941926
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Ran for 86550 frames Cell Events: 3052 mean_circ: 0.941813
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Ran for 93550 frames Cell Events: 3293 mean_circ: 0.941685
Ran for 95550 frames Cell Events: 3387 mean_circ: 0.941842
Ran for 96050 frames Cell Events: 3405 mean_circ: 0.941808
Ran for 97050 frames Cell Events: 3447 mean_circ: 0.941825
Ran for 98050 frames Cell Events: 3490 mean_circ: 0.94188
Ran for 98550 frames Cell Events: 3515 mean_circ: 0.941882
Ran for 100050 frames Cell Events: 3569 mean_circ: 0.941823
Ran for 100550 frames Cell Events: 3593 mean_circ: 0.941827
Ran for 101050 frames Cell Events: 3610 mean_circ: 0.941795
Ran for 102550 frames Cell Events: 3664 mean_circ: 0.94182
Ran for 104550 frames Cell Events: 3754 mean_circ: 0.941946
Ran for 105050 frames Cell Events: 3776 mean_circ: 0.941915
Ran for 105550 frames Cell Events: 3783 mean_circ: 0.941912
Ran for 106050 frames Cell Events: 3796 mean_circ: 0.941954
Ran for 106550 frames Cell Events: 3813 mean_circ: 0.942015
Ran for 107550 frames Cell Events: 3861 mean_circ: 0.941944
Ran for 108050 frames Cell Events: 3873 mean_circ: 0.941955
Ran for 109050 frames Cell Events: 3906 mean_circ: 0.94195
Ran for 109550 frames Cell Events: 3934 mean_circ: 0.941987
Ran for 110050 frames Cell Events: 3957 mean_circ: 0.941962
Ran for 112550 frames Cell Events: 4061 mean_circ: 0.941857
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Ran for 121550 frames Cell Events: 4409 mean_circ: 0.942012
Ran for 122550 frames Cell Events: 4467 mean_circ: 0.942067
Ran for 123050 frames Cell Events: 4482 mean_circ: 0.942071
Ran for 123550 frames Cell Events: 4503 mean_circ: 0.942075
Ran for 124050 frames Cell Events: 4523 mean_circ: 0.942031
Ran for 125550 frames Cell Events: 4568 mean_circ: 0.942014
Ran for 126050 frames Cell Events: 4589 mean_circ: 0.942006
Ran for 126550 frames Cell Events: 4606 mean_circ: 0.942067
Ran for 127550 frames Cell Events: 4637 mean_circ: 0.942079
Ran for 128550 frames Cell Events: 4666 mean_circ: 0.942008
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Ran for 131050 frames Cell Events: 4769 mean_circ: 0.941942
Ran for 131550 frames Cell Events: 4782 mean_circ: 0.94194
Ran for 132550 frames Cell Events: 4820 mean_circ: 0.941958
Ran for 134050 frames Cell Events: 4865 mean_circ: 0.941931
Ran for 136050 frames Cell Events: 4920 mean_circ: 0.941989
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Ran for 138050 frames Cell Events: 4990 mean_circ: 0.941862
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Ran for 143550 frames Cell Events: 5212 mean_circ: 0.941868
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Ran for 147050 frames Cell Events: 5311 mean_circ: 0.941858
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Ran for 149050 frames Cell Events: 5379 mean_circ: 0.941808
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Ran for 152050 frames Cell Events: 5477 mean_circ: 0.941761
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Ran for 156550 frames Cell Events: 5666 mean_circ: 0.941817
Ran for 157050 frames Cell Events: 5676 mean_circ: 0.94182
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Ran for 220550 frames Cell Events: 8067 mean_circ: 0.941595
Ran for 221550 frames Cell Events: 8108 mean_circ: 0.941624
Ran for 222050 frames Cell Events: 8126 mean_circ: 0.941612
Ran for 223050 frames Cell Events: 8157 mean_circ: 0.94163
Ran for 225550 frames Cell Events: 8263 mean_circ: 0.94157
Ran for 227050 frames Cell Events: 8317 mean_circ: 0.941525
Ran for 227550 frames Cell Events: 8335 mean_circ: 0.941516
Ran for 228050 frames Cell Events: 8355 mean_circ: 0.941505
Ran for 228550 frames Cell Events: 8373 mean_circ: 0.941515
Ran for 229050 frames Cell Events: 8388 mean_circ: 0.94147
Ran for 229550 frames Cell Events: 8408 mean_circ: 0.941432
Ran for 230050 frames Cell Events: 8435 mean_circ: 0.941445
Ran for 232550 frames Cell Events: 8560 mean_circ: 0.941452
Ran for 233050 frames Cell Events: 8582 mean_circ: 0.941446
Ran for 233550 frames Cell Events: 8602 mean_circ: 0.941447
Ran for 234050 frames Cell Events: 8616 mean_circ: 0.941454
Ran for 235050 frames Cell Events: 8655 mean_circ: 0.941499
Ran for 236050 frames Cell Events: 8694 mean_circ: 0.941495
Ran for 236550 frames Cell Events: 8712 mean_circ: 0.941517
Ran for 237550 frames Cell Events: 8748 mean_circ: 0.941512
Ran for 238050 frames Cell Events: 8765 mean_circ: 0.941501
Ran for 238550 frames Cell Events: 8785 mean_circ: 0.941501
Ran for 239050 frames Cell Events: 8802 mean_circ: 0.941464
Ran for 239550 frames Cell Events: 8815 mean_circ: 0.94148
Ran for 240050 frames Cell Events: 8831 mean_circ: 0.94146
Ran for 240550 frames Cell Events: 8850 mean_circ: 0.94146
Ran for 241050 frames Cell Events: 8859 mean_circ: 0.94148
Ran for 241550 frames Cell Events: 8878 mean_circ: 0.94149
Ran for 242050 frames Cell Events: 8909 mean_circ: 0.941466
Ran for 242550 frames Cell Events: 8939 mean_circ: 0.941443
Ran for 243050 frames Cell Events: 8961 mean_circ: 0.941456
Ran for 243550 frames Cell Events: 8993 mean_circ: 0.941448
Ran for 244550 frames Cell Events: 9026 mean_circ: 0.941463
Ran for 245050 frames Cell Events: 9048 mean_circ: 0.941461
Ran for 246050 frames Cell Events: 9091 mean_circ: 0.941389
Ran for 247550 frames Cell Events: 9156 mean_circ: 0.94129
Ran for 248050 frames Cell Events: 9185 mean_circ: 0.941283
Ran for 248550 frames Cell Events: 9210 mean_circ: 0.941277
Ran for 251050 frames Cell Events: 9308 mean_circ: 0.941235
Ran for 251550 frames Cell Events: 9332 mean_circ: 0.941227
Ran for 252050 frames Cell Events: 9354 mean_circ: 0.941217
Ran for 252550 frames Cell Events: 9375 mean_circ: 0.941169
Ran for 253050 frames Cell Events: 9394 mean_circ: 0.941178



End this measurment..



Stopping time: 2.17759e+009
****************************************
Measurement finished

Mean Circularitiy: 0.941214
Mean Area: 630.212
Mean FL-1 intensity: 3454.45
Mean FL-2 intensity: 4914.04
Mean FL-3 intensity: 0

frames processed: 253246
Total Time: 127243ms
Number of Runs (new bg img) 0
Frames collected: 254308
Skipped frames: 14807
Rescued Frames: 1400472
Samples DAQ: 63579235
Samples written: 127153846
Samples per Channel: 63576923
FPS: 1998.61

Samples per second: 499651
Expected samples/s: 500000

Events: 9448
Events per second: 74.2519
jumped back (PMT): 35
#Frames read, frequency
# 0 frames :372834
# 1 frames :116431
# 2 frames :4998
# 3 frames :2049
# 4 frames :1115
# 5 frames :577
# 6 frames :1258
# 7 frames :2046
# 8 frames :1293
# 9 frames :805
# 10 frames :1054
# 11 frames :782
# 12 frames :643
# 13 frames :372
# 14 frames :278
# 15 frames :238
# 16 frames :221
# 17 frames :190
# 18 frames :183
# 19 frames :176
# 20 frames :154
# 21 frames :130
# 22 frames :109
# 23 frames :87
# 24 frames :56
# 25 frames :53
# 26 frames :35
# 27 frames :26
# 28 frames :31
# 29 frames :27
# 30 frames :23
# 31 frames :24
# 32 frames :13
# 33 frames :11
# 34 frames :12
# 35 frames :8
# 36 frames :7
# 37 frames :5
# 38 frames :3
# 39 frames :2
# 40 frames :4
# 41 frames :6
# 42 frames :2
# 43 frames :3
# 44 frames :3
# 45 frames :7
# 46 frames :2
# 47 frames :4
# 48 frames :4
# 49 frames :1
# 50 frames :1
# 51 frames :2
# 53 frames :1
# 54 frames :4
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 60 frames :1
# 61 frames :1
# 62 frames :3
# 68 frames :1
# 72 frames :1
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.189
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0