Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_15.27.59 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6c3126b532a2031e721e161d9fa9364a",
"name": "M1_20170609_1452_dsRNA_Y5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "13:28:19"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.4272
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.18334e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.941967
Ran for 550 frames Cell Events: 8 mean_circ: 0.942392
Ran for 1050 frames Cell Events: 16 mean_circ: 0.939785
Ran for 1550 frames Cell Events: 23 mean_circ: 0.943946
Ran for 2050 frames Cell Events: 37 mean_circ: 0.934219
Ran for 2550 frames Cell Events: 52 mean_circ: 0.93369
Ran for 3050 frames Cell Events: 69 mean_circ: 0.932305
Ran for 4050 frames Cell Events: 95 mean_circ: 0.934153
Ran for 4550 frames Cell Events: 109 mean_circ: 0.935617
Ran for 5050 frames Cell Events: 124 mean_circ: 0.932396
Ran for 6050 frames Cell Events: 151 mean_circ: 0.932406
Ran for 6550 frames Cell Events: 167 mean_circ: 0.933624
Ran for 7050 frames Cell Events: 182 mean_circ: 0.932067
Ran for 7550 frames Cell Events: 195 mean_circ: 0.93275
Ran for 8050 frames Cell Events: 208 mean_circ: 0.933051
Ran for 9050 frames Cell Events: 238 mean_circ: 0.930993
Ran for 9550 frames Cell Events: 247 mean_circ: 0.931911
Ran for 10050 frames Cell Events: 259 mean_circ: 0.932685
Ran for 11050 frames Cell Events: 279 mean_circ: 0.931336
Ran for 11550 frames Cell Events: 301 mean_circ: 0.931369
Ran for 12050 frames Cell Events: 312 mean_circ: 0.931631
Ran for 12550 frames Cell Events: 325 mean_circ: 0.931923
Ran for 13050 frames Cell Events: 339 mean_circ: 0.93264
Ran for 13550 frames Cell Events: 351 mean_circ: 0.932397
Ran for 14050 frames Cell Events: 363 mean_circ: 0.932173
Ran for 14550 frames Cell Events: 379 mean_circ: 0.931827
Ran for 15050 frames Cell Events: 393 mean_circ: 0.932104
Ran for 15550 frames Cell Events: 400 mean_circ: 0.932364
Ran for 16050 frames Cell Events: 420 mean_circ: 0.932006
Ran for 16550 frames Cell Events: 437 mean_circ: 0.932505
Ran for 17050 frames Cell Events: 451 mean_circ: 0.932268
Ran for 18050 frames Cell Events: 478 mean_circ: 0.930933
Ran for 18550 frames Cell Events: 488 mean_circ: 0.93115
Ran for 19050 frames Cell Events: 504 mean_circ: 0.931607
Ran for 19550 frames Cell Events: 516 mean_circ: 0.931535
Ran for 20050 frames Cell Events: 523 mean_circ: 0.931562
Ran for 20550 frames Cell Events: 526 mean_circ: 0.931449
Ran for 21050 frames Cell Events: 537 mean_circ: 0.931492
Ran for 21550 frames Cell Events: 551 mean_circ: 0.931366
Ran for 23550 frames Cell Events: 606 mean_circ: 0.931826
Ran for 24050 frames Cell Events: 614 mean_circ: 0.931759
Ran for 24550 frames Cell Events: 617 mean_circ: 0.931692
Ran for 25050 frames Cell Events: 626 mean_circ: 0.931848
Ran for 26050 frames Cell Events: 657 mean_circ: 0.932434
Ran for 26550 frames Cell Events: 667 mean_circ: 0.93251
Ran for 27050 frames Cell Events: 683 mean_circ: 0.932442
Ran for 27550 frames Cell Events: 693 mean_circ: 0.932522
Ran for 28550 frames Cell Events: 723 mean_circ: 0.932682
Ran for 29050 frames Cell Events: 733 mean_circ: 0.932167
Ran for 29550 frames Cell Events: 753 mean_circ: 0.932284
Ran for 30050 frames Cell Events: 770 mean_circ: 0.932254
Ran for 30550 frames Cell Events: 784 mean_circ: 0.932302
Ran for 31050 frames Cell Events: 791 mean_circ: 0.932423
Ran for 31550 frames Cell Events: 808 mean_circ: 0.932528
Ran for 33050 frames Cell Events: 842 mean_circ: 0.932465
Ran for 33550 frames Cell Events: 852 mean_circ: 0.932411
Ran for 35550 frames Cell Events: 901 mean_circ: 0.932743
Ran for 36550 frames Cell Events: 930 mean_circ: 0.932896
Ran for 37050 frames Cell Events: 941 mean_circ: 0.932974
Ran for 37550 frames Cell Events: 952 mean_circ: 0.933127
Ran for 38050 frames Cell Events: 961 mean_circ: 0.933022
Ran for 38550 frames Cell Events: 974 mean_circ: 0.933037
Ran for 39050 frames Cell Events: 984 mean_circ: 0.933222
Ran for 39550 frames Cell Events: 994 mean_circ: 0.93305
Ran for 40050 frames Cell Events: 1002 mean_circ: 0.933271
Ran for 40550 frames Cell Events: 1017 mean_circ: 0.933443
Ran for 41050 frames Cell Events: 1033 mean_circ: 0.933437
Ran for 42050 frames Cell Events: 1055 mean_circ: 0.933391
Ran for 42550 frames Cell Events: 1058 mean_circ: 0.933254
Ran for 43050 frames Cell Events: 1065 mean_circ: 0.933132
Ran for 44050 frames Cell Events: 1083 mean_circ: 0.933038
Ran for 44550 frames Cell Events: 1096 mean_circ: 0.933045
Ran for 45050 frames Cell Events: 1101 mean_circ: 0.933048
Ran for 45550 frames Cell Events: 1117 mean_circ: 0.933081
Ran for 46050 frames Cell Events: 1132 mean_circ: 0.93292
Ran for 46550 frames Cell Events: 1144 mean_circ: 0.933037
Ran for 47050 frames Cell Events: 1150 mean_circ: 0.932893
Ran for 48050 frames Cell Events: 1184 mean_circ: 0.933015
Ran for 48550 frames Cell Events: 1194 mean_circ: 0.93304
Ran for 49050 frames Cell Events: 1207 mean_circ: 0.933109
Ran for 50050 frames Cell Events: 1239 mean_circ: 0.932836
Ran for 50550 frames Cell Events: 1250 mean_circ: 0.932885
Ran for 51050 frames Cell Events: 1264 mean_circ: 0.932913
Ran for 51550 frames Cell Events: 1277 mean_circ: 0.932849
Ran for 52050 frames Cell Events: 1285 mean_circ: 0.932928
Ran for 53050 frames Cell Events: 1303 mean_circ: 0.933115
Ran for 53550 frames Cell Events: 1311 mean_circ: 0.933057
Ran for 54050 frames Cell Events: 1318 mean_circ: 0.933155
Ran for 54550 frames Cell Events: 1328 mean_circ: 0.933215
Ran for 55050 frames Cell Events: 1344 mean_circ: 0.932743
Ran for 55550 frames Cell Events: 1352 mean_circ: 0.932695
Ran for 56550 frames Cell Events: 1374 mean_circ: 0.932829
Ran for 57050 frames Cell Events: 1385 mean_circ: 0.932671
Ran for 57550 frames Cell Events: 1400 mean_circ: 0.93278
Ran for 58050 frames Cell Events: 1417 mean_circ: 0.932751
Ran for 58550 frames Cell Events: 1429 mean_circ: 0.932794
Ran for 59050 frames Cell Events: 1441 mean_circ: 0.932863
Ran for 59550 frames Cell Events: 1457 mean_circ: 0.932866
Ran for 60050 frames Cell Events: 1466 mean_circ: 0.933001
Ran for 60550 frames Cell Events: 1472 mean_circ: 0.933066
Ran for 61550 frames Cell Events: 1497 mean_circ: 0.932775
Ran for 62050 frames Cell Events: 1515 mean_circ: 0.932772
Ran for 62550 frames Cell Events: 1522 mean_circ: 0.932818
Ran for 63050 frames Cell Events: 1535 mean_circ: 0.932676
Ran for 63550 frames Cell Events: 1549 mean_circ: 0.932729
Ran for 64050 frames Cell Events: 1558 mean_circ: 0.932672
Ran for 64550 frames Cell Events: 1567 mean_circ: 0.932631
Ran for 65050 frames Cell Events: 1576 mean_circ: 0.932708
Ran for 65550 frames Cell Events: 1588 mean_circ: 0.932775
Ran for 66050 frames Cell Events: 1603 mean_circ: 0.932695
Ran for 66550 frames Cell Events: 1619 mean_circ: 0.932615
Ran for 67050 frames Cell Events: 1633 mean_circ: 0.93261
Ran for 67550 frames Cell Events: 1647 mean_circ: 0.932671
Ran for 68550 frames Cell Events: 1671 mean_circ: 0.932693
Ran for 69550 frames Cell Events: 1698 mean_circ: 0.932708
Ran for 70050 frames Cell Events: 1711 mean_circ: 0.932754
Ran for 70550 frames Cell Events: 1718 mean_circ: 0.932685
Ran for 71050 frames Cell Events: 1726 mean_circ: 0.932687
Ran for 72050 frames Cell Events: 1747 mean_circ: 0.932672
Ran for 72550 frames Cell Events: 1760 mean_circ: 0.932769
Ran for 73550 frames Cell Events: 1787 mean_circ: 0.932745
Ran for 74050 frames Cell Events: 1797 mean_circ: 0.932548
Ran for 74550 frames Cell Events: 1812 mean_circ: 0.932551
Ran for 75050 frames Cell Events: 1817 mean_circ: 0.932696
Ran for 75550 frames Cell Events: 1831 mean_circ: 0.932773
Ran for 76050 frames Cell Events: 1840 mean_circ: 0.932923
Ran for 76550 frames Cell Events: 1855 mean_circ: 0.932925
Ran for 77050 frames Cell Events: 1869 mean_circ: 0.932812
Ran for 78550 frames Cell Events: 1921 mean_circ: 0.932752
Ran for 79050 frames Cell Events: 1933 mean_circ: 0.932766
Ran for 79550 frames Cell Events: 1945 mean_circ: 0.932696
Ran for 80550 frames Cell Events: 1975 mean_circ: 0.932688
Ran for 81550 frames Cell Events: 2007 mean_circ: 0.93272
Ran for 82050 frames Cell Events: 2020 mean_circ: 0.932648
Ran for 82550 frames Cell Events: 2033 mean_circ: 0.932737
Ran for 83050 frames Cell Events: 2042 mean_circ: 0.93272
Ran for 83550 frames Cell Events: 2056 mean_circ: 0.932766
Ran for 84050 frames Cell Events: 2068 mean_circ: 0.932884
Ran for 84550 frames Cell Events: 2080 mean_circ: 0.93286
Ran for 85050 frames Cell Events: 2096 mean_circ: 0.9329
Ran for 86050 frames Cell Events: 2124 mean_circ: 0.932974
Ran for 86550 frames Cell Events: 2133 mean_circ: 0.933035
Ran for 87050 frames Cell Events: 2153 mean_circ: 0.932909
Ran for 87550 frames Cell Events: 2160 mean_circ: 0.932915
Ran for 88050 frames Cell Events: 2169 mean_circ: 0.933019
Ran for 89050 frames Cell Events: 2189 mean_circ: 0.932995
Ran for 89550 frames Cell Events: 2202 mean_circ: 0.93302
Ran for 90050 frames Cell Events: 2218 mean_circ: 0.932948
Ran for 90550 frames Cell Events: 2235 mean_circ: 0.933008
Ran for 91550 frames Cell Events: 2261 mean_circ: 0.933049
Ran for 92050 frames Cell Events: 2274 mean_circ: 0.932933
Ran for 92550 frames Cell Events: 2282 mean_circ: 0.932948
Ran for 93050 frames Cell Events: 2300 mean_circ: 0.933045
Ran for 94050 frames Cell Events: 2334 mean_circ: 0.933084
Ran for 95050 frames Cell Events: 2357 mean_circ: 0.933004
Ran for 96050 frames Cell Events: 2388 mean_circ: 0.933007
Ran for 96550 frames Cell Events: 2397 mean_circ: 0.932957
Ran for 97050 frames Cell Events: 2410 mean_circ: 0.933002
Ran for 97550 frames Cell Events: 2419 mean_circ: 0.933025
Ran for 98050 frames Cell Events: 2434 mean_circ: 0.932934
Ran for 98550 frames Cell Events: 2445 mean_circ: 0.932911
Ran for 99550 frames Cell Events: 2475 mean_circ: 0.932906
Ran for 100550 frames Cell Events: 2501 mean_circ: 0.933089
Ran for 101050 frames Cell Events: 2518 mean_circ: 0.933077
Ran for 101550 frames Cell Events: 2530 mean_circ: 0.933112
Ran for 102050 frames Cell Events: 2547 mean_circ: 0.933154
Ran for 102550 frames Cell Events: 2555 mean_circ: 0.933183
Ran for 103050 frames Cell Events: 2562 mean_circ: 0.933222
Ran for 104050 frames Cell Events: 2586 mean_circ: 0.933238
Ran for 104550 frames Cell Events: 2590 mean_circ: 0.933212
Ran for 105050 frames Cell Events: 2596 mean_circ: 0.933249
Ran for 105550 frames Cell Events: 2607 mean_circ: 0.933048
Ran for 106550 frames Cell Events: 2631 mean_circ: 0.93311
Ran for 107050 frames Cell Events: 2642 mean_circ: 0.933117
Ran for 108050 frames Cell Events: 2670 mean_circ: 0.933104
Ran for 108550 frames Cell Events: 2688 mean_circ: 0.933029
Ran for 109050 frames Cell Events: 2701 mean_circ: 0.932988
Ran for 109550 frames Cell Events: 2713 mean_circ: 0.932983
Ran for 110550 frames Cell Events: 2748 mean_circ: 0.932966
Ran for 111050 frames Cell Events: 2765 mean_circ: 0.933058
Ran for 111550 frames Cell Events: 2774 mean_circ: 0.933066
Ran for 112050 frames Cell Events: 2787 mean_circ: 0.933123
Ran for 112550 frames Cell Events: 2804 mean_circ: 0.933069
Ran for 113550 frames Cell Events: 2830 mean_circ: 0.933033
Ran for 114550 frames Cell Events: 2852 mean_circ: 0.933118
Ran for 115050 frames Cell Events: 2857 mean_circ: 0.933165
Ran for 115550 frames Cell Events: 2871 mean_circ: 0.933199
Ran for 117050 frames Cell Events: 2919 mean_circ: 0.933213
Ran for 117550 frames Cell Events: 2931 mean_circ: 0.933256
Ran for 118050 frames Cell Events: 2949 mean_circ: 0.933364
Ran for 118550 frames Cell Events: 2960 mean_circ: 0.933359
Ran for 119050 frames Cell Events: 2972 mean_circ: 0.933311
Ran for 119550 frames Cell Events: 2985 mean_circ: 0.933223
Ran for 120050 frames Cell Events: 2997 mean_circ: 0.933103
Ran for 120550 frames Cell Events: 3004 mean_circ: 0.933119
Ran for 121050 frames Cell Events: 3012 mean_circ: 0.933142
Ran for 122050 frames Cell Events: 3037 mean_circ: 0.933195
Ran for 122550 frames Cell Events: 3044 mean_circ: 0.933169
Ran for 123550 frames Cell Events: 3066 mean_circ: 0.933159
Ran for 124050 frames Cell Events: 3086 mean_circ: 0.933147
Ran for 125550 frames Cell Events: 3116 mean_circ: 0.933178
Ran for 126550 frames Cell Events: 3138 mean_circ: 0.93312
Ran for 128050 frames Cell Events: 3185 mean_circ: 0.933134
Ran for 128550 frames Cell Events: 3201 mean_circ: 0.932999
Ran for 129050 frames Cell Events: 3212 mean_circ: 0.933041
Ran for 129550 frames Cell Events: 3223 mean_circ: 0.933037
Ran for 130550 frames Cell Events: 3248 mean_circ: 0.932992
Ran for 131050 frames Cell Events: 3258 mean_circ: 0.932992
Ran for 132050 frames Cell Events: 3286 mean_circ: 0.933035
Ran for 132550 frames Cell Events: 3295 mean_circ: 0.933057
Ran for 133050 frames Cell Events: 3311 mean_circ: 0.933005
Ran for 133550 frames Cell Events: 3325 mean_circ: 0.933033
Ran for 134050 frames Cell Events: 3331 mean_circ: 0.933005
Ran for 134550 frames Cell Events: 3343 mean_circ: 0.933015
Ran for 135050 frames Cell Events: 3356 mean_circ: 0.932988
Ran for 135550 frames Cell Events: 3372 mean_circ: 0.93296
Ran for 136550 frames Cell Events: 3393 mean_circ: 0.932899
Ran for 137050 frames Cell Events: 3404 mean_circ: 0.932897
Ran for 137550 frames Cell Events: 3418 mean_circ: 0.932903
Ran for 138050 frames Cell Events: 3433 mean_circ: 0.932902
Ran for 138550 frames Cell Events: 3442 mean_circ: 0.932937
Ran for 139050 frames Cell Events: 3458 mean_circ: 0.932984
Ran for 139550 frames Cell Events: 3477 mean_circ: 0.932994
Ran for 140050 frames Cell Events: 3488 mean_circ: 0.932937
Ran for 140550 frames Cell Events: 3500 mean_circ: 0.933
Ran for 141050 frames Cell Events: 3515 mean_circ: 0.932943
Ran for 141550 frames Cell Events: 3524 mean_circ: 0.932977
Ran for 142050 frames Cell Events: 3533 mean_circ: 0.932955
Ran for 142550 frames Cell Events: 3540 mean_circ: 0.932923
Ran for 143050 frames Cell Events: 3549 mean_circ: 0.932941
Ran for 144050 frames Cell Events: 3573 mean_circ: 0.933004
Ran for 144550 frames Cell Events: 3582 mean_circ: 0.932923
Ran for 145550 frames Cell Events: 3600 mean_circ: 0.932843
Ran for 146050 frames Cell Events: 3618 mean_circ: 0.93288
Ran for 147050 frames Cell Events: 3649 mean_circ: 0.932759
Ran for 147550 frames Cell Events: 3661 mean_circ: 0.932724
Ran for 148550 frames Cell Events: 3691 mean_circ: 0.932626
Ran for 149050 frames Cell Events: 3699 mean_circ: 0.932647
Ran for 149550 frames Cell Events: 3716 mean_circ: 0.932724
Ran for 150050 frames Cell Events: 3732 mean_circ: 0.932748
Ran for 151050 frames Cell Events: 3769 mean_circ: 0.932724
Ran for 151550 frames Cell Events: 3777 mean_circ: 0.93274
Ran for 152550 frames Cell Events: 3807 mean_circ: 0.932749
Ran for 153050 frames Cell Events: 3818 mean_circ: 0.932778
Ran for 154050 frames Cell Events: 3847 mean_circ: 0.932749
Ran for 154550 frames Cell Events: 3860 mean_circ: 0.932764
Ran for 155550 frames Cell Events: 3895 mean_circ: 0.932697
Ran for 156050 frames Cell Events: 3913 mean_circ: 0.932704
Ran for 156550 frames Cell Events: 3924 mean_circ: 0.932713
Ran for 157050 frames Cell Events: 3938 mean_circ: 0.93274
Ran for 157550 frames Cell Events: 3957 mean_circ: 0.932802
Ran for 158050 frames Cell Events: 3974 mean_circ: 0.932806
Ran for 158550 frames Cell Events: 3998 mean_circ: 0.932747
Ran for 159050 frames Cell Events: 4026 mean_circ: 0.932755
Ran for 160050 frames Cell Events: 4064 mean_circ: 0.932723
Ran for 160550 frames Cell Events: 4074 mean_circ: 0.932749
Ran for 161050 frames Cell Events: 4083 mean_circ: 0.932774
Ran for 161550 frames Cell Events: 4090 mean_circ: 0.932798
Ran for 162050 frames Cell Events: 4102 mean_circ: 0.93279
Ran for 163050 frames Cell Events: 4126 mean_circ: 0.932733
Ran for 163550 frames Cell Events: 4132 mean_circ: 0.932742
Ran for 164050 frames Cell Events: 4140 mean_circ: 0.932731
Ran for 164550 frames Cell Events: 4154 mean_circ: 0.932755
Ran for 165050 frames Cell Events: 4160 mean_circ: 0.932769
Ran for 165550 frames Cell Events: 4168 mean_circ: 0.93278
Ran for 166550 frames Cell Events: 4201 mean_circ: 0.93266
Ran for 167550 frames Cell Events: 4226 mean_circ: 0.932622
Ran for 168050 frames Cell Events: 4239 mean_circ: 0.932601
Ran for 168550 frames Cell Events: 4255 mean_circ: 0.932591
Ran for 169050 frames Cell Events: 4267 mean_circ: 0.932574
Ran for 169550 frames Cell Events: 4281 mean_circ: 0.932508
Ran for 170050 frames Cell Events: 4297 mean_circ: 0.932456
Ran for 170550 frames Cell Events: 4308 mean_circ: 0.932516
Ran for 171050 frames Cell Events: 4322 mean_circ: 0.93254
Ran for 171550 frames Cell Events: 4330 mean_circ: 0.932538
Ran for 172050 frames Cell Events: 4334 mean_circ: 0.932529
Ran for 172550 frames Cell Events: 4345 mean_circ: 0.932523
Ran for 173550 frames Cell Events: 4370 mean_circ: 0.932479
Ran for 174550 frames Cell Events: 4403 mean_circ: 0.932436
Ran for 175050 frames Cell Events: 4427 mean_circ: 0.932469
Ran for 175550 frames Cell Events: 4441 mean_circ: 0.932288
Ran for 176050 frames Cell Events: 4450 mean_circ: 0.932328
Ran for 176550 frames Cell Events: 4460 mean_circ: 0.932318
Ran for 177050 frames Cell Events: 4470 mean_circ: 0.93231
Ran for 177550 frames Cell Events: 4481 mean_circ: 0.932338
Ran for 178050 frames Cell Events: 4495 mean_circ: 0.93239
Ran for 178550 frames Cell Events: 4511 mean_circ: 0.932368
Ran for 179550 frames Cell Events: 4527 mean_circ: 0.932337
Ran for 181050 frames Cell Events: 4565 mean_circ: 0.932344
Ran for 182050 frames Cell Events: 4590 mean_circ: 0.932366
Ran for 182550 frames Cell Events: 4599 mean_circ: 0.932375
Ran for 183050 frames Cell Events: 4613 mean_circ: 0.932368
Ran for 183550 frames Cell Events: 4626 mean_circ: 0.932365
Ran for 184050 frames Cell Events: 4642 mean_circ: 0.932359
Ran for 184550 frames Cell Events: 4662 mean_circ: 0.932337
Ran for 186050 frames Cell Events: 4713 mean_circ: 0.932395
Ran for 186550 frames Cell Events: 4725 mean_circ: 0.932406
Ran for 187050 frames Cell Events: 4738 mean_circ: 0.932439
Ran for 187550 frames Cell Events: 4746 mean_circ: 0.93242
Ran for 188050 frames Cell Events: 4762 mean_circ: 0.932481
Ran for 188550 frames Cell Events: 4780 mean_circ: 0.932396
Ran for 189050 frames Cell Events: 4791 mean_circ: 0.932373
Ran for 189550 frames Cell Events: 4801 mean_circ: 0.932402
Ran for 190050 frames Cell Events: 4811 mean_circ: 0.932431
Ran for 191050 frames Cell Events: 4851 mean_circ: 0.932352
Ran for 191550 frames Cell Events: 4864 mean_circ: 0.932368
Ran for 192050 frames Cell Events: 4874 mean_circ: 0.932376
Ran for 193050 frames Cell Events: 4894 mean_circ: 0.932397
Ran for 193550 frames Cell Events: 4906 mean_circ: 0.932365
Ran for 194550 frames Cell Events: 4934 mean_circ: 0.932338
Ran for 195050 frames Cell Events: 4949 mean_circ: 0.932265
Ran for 196050 frames Cell Events: 4972 mean_circ: 0.932161
Ran for 196550 frames Cell Events: 4975 mean_circ: 0.932181
Ran for 199050 frames Cell Events: 5041 mean_circ: 0.932154
Ran for 199550 frames Cell Events: 5059 mean_circ: 0.932092
Ran for 200050 frames Cell Events: 5070 mean_circ: 0.932068
Ran for 200550 frames Cell Events: 5085 mean_circ: 0.932077
Ran for 201050 frames Cell Events: 5096 mean_circ: 0.932072
Ran for 202050 frames Cell Events: 5121 mean_circ: 0.932122
Ran for 202550 frames Cell Events: 5141 mean_circ: 0.932092
Ran for 203050 frames Cell Events: 5150 mean_circ: 0.932099
Ran for 203550 frames Cell Events: 5170 mean_circ: 0.932097
Ran for 204050 frames Cell Events: 5190 mean_circ: 0.932013
Ran for 205050 frames Cell Events: 5219 mean_circ: 0.932045
Ran for 205550 frames Cell Events: 5232 mean_circ: 0.932028
Ran for 206050 frames Cell Events: 5244 mean_circ: 0.932056
Ran for 206550 frames Cell Events: 5252 mean_circ: 0.932056
Ran for 207050 frames Cell Events: 5261 mean_circ: 0.932071
Ran for 207550 frames Cell Events: 5273 mean_circ: 0.932081
Ran for 208050 frames Cell Events: 5288 mean_circ: 0.93204
Ran for 208550 frames Cell Events: 5302 mean_circ: 0.932085
Ran for 209050 frames Cell Events: 5311 mean_circ: 0.932112
Ran for 209550 frames Cell Events: 5316 mean_circ: 0.932119
Ran for 210050 frames Cell Events: 5327 mean_circ: 0.93213
Ran for 210550 frames Cell Events: 5336 mean_circ: 0.93215
Ran for 211550 frames Cell Events: 5359 mean_circ: 0.932102
Ran for 212050 frames Cell Events: 5380 mean_circ: 0.932084
Ran for 213550 frames Cell Events: 5417 mean_circ: 0.932076
Ran for 214050 frames Cell Events: 5432 mean_circ: 0.932077
Ran for 215550 frames Cell Events: 5471 mean_circ: 0.93209
Ran for 216550 frames Cell Events: 5500 mean_circ: 0.93212
Ran for 217550 frames Cell Events: 5529 mean_circ: 0.932121
Ran for 219050 frames Cell Events: 5570 mean_circ: 0.932155
Ran for 219550 frames Cell Events: 5581 mean_circ: 0.932063
Ran for 220550 frames Cell Events: 5598 mean_circ: 0.932089
Ran for 221050 frames Cell Events: 5610 mean_circ: 0.932131
Ran for 221550 frames Cell Events: 5620 mean_circ: 0.932141
Ran for 222550 frames Cell Events: 5639 mean_circ: 0.932143
Ran for 223550 frames Cell Events: 5672 mean_circ: 0.932136
Ran for 224050 frames Cell Events: 5691 mean_circ: 0.932158
Ran for 225050 frames Cell Events: 5726 mean_circ: 0.932128
Ran for 225550 frames Cell Events: 5732 mean_circ: 0.932111
Ran for 226050 frames Cell Events: 5739 mean_circ: 0.932109
Ran for 226550 frames Cell Events: 5746 mean_circ: 0.932117
Ran for 227050 frames Cell Events: 5764 mean_circ: 0.93214
Ran for 227550 frames Cell Events: 5776 mean_circ: 0.93212
Ran for 228050 frames Cell Events: 5793 mean_circ: 0.93213
Ran for 228550 frames Cell Events: 5800 mean_circ: 0.93214
Ran for 229050 frames Cell Events: 5808 mean_circ: 0.932163
Ran for 229550 frames Cell Events: 5821 mean_circ: 0.932116
Ran for 230050 frames Cell Events: 5832 mean_circ: 0.93212
Ran for 230550 frames Cell Events: 5841 mean_circ: 0.932081
Ran for 231050 frames Cell Events: 5853 mean_circ: 0.932055
Ran for 231550 frames Cell Events: 5871 mean_circ: 0.931964
Ran for 232550 frames Cell Events: 5896 mean_circ: 0.931967
Ran for 233550 frames Cell Events: 5919 mean_circ: 0.932013
Ran for 234050 frames Cell Events: 5931 mean_circ: 0.932032
Ran for 234550 frames Cell Events: 5943 mean_circ: 0.932028
Ran for 235550 frames Cell Events: 5961 mean_circ: 0.932009
Ran for 236550 frames Cell Events: 5991 mean_circ: 0.932051
Ran for 237050 frames Cell Events: 6005 mean_circ: 0.932056
Ran for 237550 frames Cell Events: 6017 mean_circ: 0.932008
Ran for 238050 frames Cell Events: 6030 mean_circ: 0.931991
Ran for 238550 frames Cell Events: 6042 mean_circ: 0.932006
Ran for 239050 frames Cell Events: 6056 mean_circ: 0.932036
Ran for 239550 frames Cell Events: 6071 mean_circ: 0.932059
Ran for 240050 frames Cell Events: 6088 mean_circ: 0.932075
Ran for 240550 frames Cell Events: 6099 mean_circ: 0.932064
Ran for 241050 frames Cell Events: 6113 mean_circ: 0.932032
Ran for 241550 frames Cell Events: 6125 mean_circ: 0.932025
Ran for 242050 frames Cell Events: 6138 mean_circ: 0.93204
Ran for 242550 frames Cell Events: 6154 mean_circ: 0.932075
Ran for 243550 frames Cell Events: 6180 mean_circ: 0.93209
Ran for 244550 frames Cell Events: 6209 mean_circ: 0.932127
Ran for 245050 frames Cell Events: 6220 mean_circ: 0.932122
Ran for 245550 frames Cell Events: 6243 mean_circ: 0.932064
Ran for 246050 frames Cell Events: 6254 mean_circ: 0.932108
Ran for 246550 frames Cell Events: 6269 mean_circ: 0.93212
Ran for 247550 frames Cell Events: 6294 mean_circ: 0.932121
Ran for 248050 frames Cell Events: 6306 mean_circ: 0.932027
Ran for 248550 frames Cell Events: 6321 mean_circ: 0.93204
Ran for 249050 frames Cell Events: 6326 mean_circ: 0.932035
Ran for 249550 frames Cell Events: 6333 mean_circ: 0.932042
Ran for 250050 frames Cell Events: 6345 mean_circ: 0.932003
Ran for 250550 frames Cell Events: 6360 mean_circ: 0.931998
Ran for 251050 frames Cell Events: 6373 mean_circ: 0.932017
Ran for 251550 frames Cell Events: 6388 mean_circ: 0.932007
Ran for 252050 frames Cell Events: 6405 mean_circ: 0.932034
Ran for 252550 frames Cell Events: 6418 mean_circ: 0.932045
Ran for 253050 frames Cell Events: 6434 mean_circ: 0.932047
Ran for 253550 frames Cell Events: 6447 mean_circ: 0.932034
Ran for 254050 frames Cell Events: 6456 mean_circ: 0.932009
Ran for 254550 frames Cell Events: 6464 mean_circ: 0.932028
Ran for 255050 frames Cell Events: 6477 mean_circ: 0.932034
Ran for 256050 frames Cell Events: 6513 mean_circ: 0.931996
Ran for 256550 frames Cell Events: 6528 mean_circ: 0.931995
Ran for 257050 frames Cell Events: 6542 mean_circ: 0.932007
Ran for 257550 frames Cell Events: 6560 mean_circ: 0.932014
Ran for 258050 frames Cell Events: 6574 mean_circ: 0.931967
Ran for 259550 frames Cell Events: 6622 mean_circ: 0.931978
Ran for 260050 frames Cell Events: 6634 mean_circ: 0.931995
Ran for 260550 frames Cell Events: 6650 mean_circ: 0.931987
Ran for 261050 frames Cell Events: 6662 mean_circ: 0.931982
Ran for 262050 frames Cell Events: 6692 mean_circ: 0.931983
Ran for 262550 frames Cell Events: 6713 mean_circ: 0.931973
Ran for 263050 frames Cell Events: 6725 mean_circ: 0.931971
Ran for 264050 frames Cell Events: 6757 mean_circ: 0.931976
Ran for 264550 frames Cell Events: 6772 mean_circ: 0.931981
Ran for 265050 frames Cell Events: 6784 mean_circ: 0.931964
Ran for 265550 frames Cell Events: 6789 mean_circ: 0.931966
Ran for 266050 frames Cell Events: 6799 mean_circ: 0.931965
Ran for 266550 frames Cell Events: 6812 mean_circ: 0.931988
Ran for 267050 frames Cell Events: 6830 mean_circ: 0.931985
Ran for 267550 frames Cell Events: 6840 mean_circ: 0.931971
Ran for 268050 frames Cell Events: 6853 mean_circ: 0.93198
Ran for 268550 frames Cell Events: 6859 mean_circ: 0.932001
Ran for 269050 frames Cell Events: 6870 mean_circ: 0.932006
Ran for 269550 frames Cell Events: 6889 mean_circ: 0.931993
Ran for 270050 frames Cell Events: 6909 mean_circ: 0.931976
Ran for 270550 frames Cell Events: 6920 mean_circ: 0.932013
Ran for 271050 frames Cell Events: 6934 mean_circ: 0.93198
Ran for 271550 frames Cell Events: 6949 mean_circ: 0.932009
Ran for 272550 frames Cell Events: 6980 mean_circ: 0.931999
Ran for 273050 frames Cell Events: 6997 mean_circ: 0.931988
Ran for 273550 frames Cell Events: 7010 mean_circ: 0.931992
Ran for 274050 frames Cell Events: 7025 mean_circ: 0.932014
Ran for 274550 frames Cell Events: 7034 mean_circ: 0.932015
Ran for 275050 frames Cell Events: 7044 mean_circ: 0.931981
Ran for 275550 frames Cell Events: 7059 mean_circ: 0.931971
Ran for 276050 frames Cell Events: 7069 mean_circ: 0.931998
Ran for 276550 frames Cell Events: 7081 mean_circ: 0.932034
Ran for 277050 frames Cell Events: 7093 mean_circ: 0.932037
Ran for 277550 frames Cell Events: 7099 mean_circ: 0.932034
Ran for 278050 frames Cell Events: 7117 mean_circ: 0.932006
Ran for 279050 frames Cell Events: 7140 mean_circ: 0.9319
Ran for 282050 frames Cell Events: 7202 mean_circ: 0.931811
Ran for 283050 frames Cell Events: 7233 mean_circ: 0.931731
Ran for 283550 frames Cell Events: 7243 mean_circ: 0.931752
Ran for 284050 frames Cell Events: 7255 mean_circ: 0.931753
Ran for 284550 frames Cell Events: 7262 mean_circ: 0.931762
Ran for 285050 frames Cell Events: 7275 mean_circ: 0.93177
Ran for 285550 frames Cell Events: 7286 mean_circ: 0.931768
Ran for 286550 frames Cell Events: 7309 mean_circ: 0.931743
Ran for 287050 frames Cell Events: 7318 mean_circ: 0.931732
Ran for 288050 frames Cell Events: 7342 mean_circ: 0.931743
Ran for 289050 frames Cell Events: 7363 mean_circ: 0.931748
Ran for 289550 frames Cell Events: 7376 mean_circ: 0.93172
Ran for 290050 frames Cell Events: 7387 mean_circ: 0.931734
Ran for 290550 frames Cell Events: 7397 mean_circ: 0.93174
Ran for 291050 frames Cell Events: 7412 mean_circ: 0.931764
Ran for 291550 frames Cell Events: 7424 mean_circ: 0.931775
Ran for 292050 frames Cell Events: 7431 mean_circ: 0.931772
Ran for 292550 frames Cell Events: 7454 mean_circ: 0.931743
Ran for 293050 frames Cell Events: 7464 mean_circ: 0.931746
Ran for 293550 frames Cell Events: 7473 mean_circ: 0.931738
Ran for 294050 frames Cell Events: 7480 mean_circ: 0.931755
Ran for 295050 frames Cell Events: 7506 mean_circ: 0.931717
Ran for 295550 frames Cell Events: 7521 mean_circ: 0.931726
Ran for 296050 frames Cell Events: 7538 mean_circ: 0.93174
Ran for 296550 frames Cell Events: 7546 mean_circ: 0.931722
Ran for 297050 frames Cell Events: 7564 mean_circ: 0.931752
Ran for 297550 frames Cell Events: 7579 mean_circ: 0.931764
Ran for 298050 frames Cell Events: 7599 mean_circ: 0.931785
Ran for 298550 frames Cell Events: 7607 mean_circ: 0.931763
Ran for 299050 frames Cell Events: 7614 mean_circ: 0.931765
Ran for 299550 frames Cell Events: 7631 mean_circ: 0.931733
Ran for 300050 frames Cell Events: 7650 mean_circ: 0.931688
Ran for 300550 frames Cell Events: 7660 mean_circ: 0.931728
Ran for 301050 frames Cell Events: 7675 mean_circ: 0.931723
Ran for 301550 frames Cell Events: 7690 mean_circ: 0.931684
Ran for 302050 frames Cell Events: 7706 mean_circ: 0.931681
Ran for 302550 frames Cell Events: 7716 mean_circ: 0.931674
Ran for 303050 frames Cell Events: 7730 mean_circ: 0.931664
Ran for 303550 frames Cell Events: 7739 mean_circ: 0.931657
Ran for 304050 frames Cell Events: 7748 mean_circ: 0.931654
Ran for 304550 frames Cell Events: 7758 mean_circ: 0.93165
Ran for 305550 frames Cell Events: 7786 mean_circ: 0.93162
Ran for 306050 frames Cell Events: 7797 mean_circ: 0.931606
Ran for 307050 frames Cell Events: 7822 mean_circ: 0.931651
Ran for 307550 frames Cell Events: 7829 mean_circ: 0.931667
Ran for 308050 frames Cell Events: 7836 mean_circ: 0.931652
Ran for 308550 frames Cell Events: 7843 mean_circ: 0.931623
Ran for 309050 frames Cell Events: 7853 mean_circ: 0.931634
Ran for 309550 frames Cell Events: 7867 mean_circ: 0.931645
Ran for 310050 frames Cell Events: 7880 mean_circ: 0.931671
Ran for 310550 frames Cell Events: 7888 mean_circ: 0.93163
Ran for 311550 frames Cell Events: 7922 mean_circ: 0.93162
Ran for 312050 frames Cell Events: 7934 mean_circ: 0.931621
Ran for 312550 frames Cell Events: 7943 mean_circ: 0.931609
Ran for 313050 frames Cell Events: 7950 mean_circ: 0.931612
Ran for 313550 frames Cell Events: 7966 mean_circ: 0.93159
Ran for 314050 frames Cell Events: 7975 mean_circ: 0.931612
Ran for 314550 frames Cell Events: 7984 mean_circ: 0.93161
Ran for 315050 frames Cell Events: 7992 mean_circ: 0.931587
Ran for 315550 frames Cell Events: 8011 mean_circ: 0.931559
Ran for 316050 frames Cell Events: 8020 mean_circ: 0.93153
Ran for 316550 frames Cell Events: 8036 mean_circ: 0.931509
Ran for 317050 frames Cell Events: 8047 mean_circ: 0.931531



End this measurment..



Stopping time: 2.1835e+009
****************************************
Measurement finished

Mean Circularitiy: 0.931531
Mean Area: 699.804
Mean FL-1 intensity: 3153.6
Mean FL-2 intensity: 5016.41
Mean FL-3 intensity: 0

frames processed: 317475
Total Time: 158884ms
Number of Runs (new bg img) 0
Frames collected: 317657
Skipped frames: 36151
Rescued Frames: 3285700
Samples DAQ: 79414284
Samples written: 158828224
Samples per Channel: 79414112
FPS: 1999.3

Samples per second: 499824
Expected samples/s: 500000

Events: 8065
Events per second: 50.7603
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :427836
# 1 frames :186161
# 2 frames :6036
# 3 frames :2534
# 4 frames :1342
# 5 frames :808
# 6 frames :1256
# 7 frames :2135
# 8 frames :1257
# 9 frames :904
# 10 frames :1241
# 11 frames :1282
# 12 frames :968
# 13 frames :404
# 14 frames :200
# 15 frames :171
# 16 frames :131
# 17 frames :119
# 18 frames :95
# 19 frames :68
# 20 frames :47
# 21 frames :45
# 22 frames :24
# 23 frames :23
# 24 frames :20
# 25 frames :19
# 26 frames :7
# 27 frames :6
# 28 frames :13
# 29 frames :8
# 30 frames :5
# 31 frames :3
# 32 frames :2
# 33 frames :7
# 34 frames :1
# 35 frames :2
# 37 frames :1
# 38 frames :1
# 44 frames :1
# 45 frames :1
# 50 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.4272
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0