Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.15.45 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3d9d97ce1cce941a10b5b2197d4f3c2c",
"name": "M1_20170611_1721_dsRNA_P8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:16:06"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8352 (max 8354) in '8352'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0269
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.36511e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 34 mean_circ: 0.920227
Ran for 1550 frames Cell Events: 46 mean_circ: 0.923848
Ran for 2050 frames Cell Events: 57 mean_circ: 0.929389
Ran for 3050 frames Cell Events: 77 mean_circ: 0.936667
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Ran for 4550 frames Cell Events: 112 mean_circ: 0.943438
Ran for 6550 frames Cell Events: 173 mean_circ: 0.94895
Ran for 7050 frames Cell Events: 186 mean_circ: 0.94975
Ran for 8050 frames Cell Events: 213 mean_circ: 0.949242
Ran for 8550 frames Cell Events: 221 mean_circ: 0.948905
Ran for 9050 frames Cell Events: 235 mean_circ: 0.948804
Ran for 9550 frames Cell Events: 249 mean_circ: 0.948499
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Ran for 10550 frames Cell Events: 269 mean_circ: 0.946767
Ran for 11050 frames Cell Events: 286 mean_circ: 0.947493
Ran for 13550 frames Cell Events: 343 mean_circ: 0.948076
Ran for 14550 frames Cell Events: 370 mean_circ: 0.948143
Ran for 15050 frames Cell Events: 384 mean_circ: 0.947986
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Ran for 73550 frames Cell Events: 1894 mean_circ: 0.951169
Ran for 74050 frames Cell Events: 1901 mean_circ: 0.95117
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Ran for 76050 frames Cell Events: 1954 mean_circ: 0.951371
Ran for 76550 frames Cell Events: 1965 mean_circ: 0.951396
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Ran for 92050 frames Cell Events: 2383 mean_circ: 0.95106
Ran for 93550 frames Cell Events: 2421 mean_circ: 0.951067
Ran for 94550 frames Cell Events: 2443 mean_circ: 0.951141
Ran for 95050 frames Cell Events: 2458 mean_circ: 0.951158
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Ran for 101050 frames Cell Events: 2615 mean_circ: 0.951157
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Ran for 122050 frames Cell Events: 3197 mean_circ: 0.951541
Ran for 123050 frames Cell Events: 3228 mean_circ: 0.951613
Ran for 123550 frames Cell Events: 3240 mean_circ: 0.951636
Ran for 124050 frames Cell Events: 3259 mean_circ: 0.951676
Ran for 124550 frames Cell Events: 3278 mean_circ: 0.951707
Ran for 125050 frames Cell Events: 3291 mean_circ: 0.951716
Ran for 125550 frames Cell Events: 3308 mean_circ: 0.951711
Ran for 126550 frames Cell Events: 3340 mean_circ: 0.951673
Ran for 127550 frames Cell Events: 3363 mean_circ: 0.95168
Ran for 128050 frames Cell Events: 3380 mean_circ: 0.951691
Ran for 128550 frames Cell Events: 3395 mean_circ: 0.951681
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Ran for 142050 frames Cell Events: 3777 mean_circ: 0.951869
Ran for 142550 frames Cell Events: 3790 mean_circ: 0.951842
Ran for 143050 frames Cell Events: 3806 mean_circ: 0.951811
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Ran for 204550 frames Cell Events: 5582 mean_circ: 0.951743
Ran for 206050 frames Cell Events: 5626 mean_circ: 0.951787
Ran for 207550 frames Cell Events: 5660 mean_circ: 0.951801
Ran for 208050 frames Cell Events: 5675 mean_circ: 0.951754
Ran for 208550 frames Cell Events: 5694 mean_circ: 0.951808
Ran for 209050 frames Cell Events: 5719 mean_circ: 0.95181
Ran for 210050 frames Cell Events: 5752 mean_circ: 0.951815
Ran for 210550 frames Cell Events: 5763 mean_circ: 0.951844
Ran for 211050 frames Cell Events: 5778 mean_circ: 0.951852
Ran for 211550 frames Cell Events: 5791 mean_circ: 0.951827
Ran for 212050 frames Cell Events: 5801 mean_circ: 0.95185
Ran for 212550 frames Cell Events: 5812 mean_circ: 0.951855
Ran for 213050 frames Cell Events: 5823 mean_circ: 0.951877
Ran for 213550 frames Cell Events: 5831 mean_circ: 0.9519
Ran for 214050 frames Cell Events: 5848 mean_circ: 0.951906
Ran for 214550 frames Cell Events: 5870 mean_circ: 0.951906
Ran for 215050 frames Cell Events: 5895 mean_circ: 0.951938
Ran for 215550 frames Cell Events: 5915 mean_circ: 0.951931
Ran for 216050 frames Cell Events: 5938 mean_circ: 0.95192
Ran for 216550 frames Cell Events: 5952 mean_circ: 0.951947
Ran for 217050 frames Cell Events: 5970 mean_circ: 0.951922
Ran for 218050 frames Cell Events: 6002 mean_circ: 0.951911
Ran for 218550 frames Cell Events: 6015 mean_circ: 0.951929
Ran for 219550 frames Cell Events: 6030 mean_circ: 0.951907
Ran for 220050 frames Cell Events: 6049 mean_circ: 0.951922
Ran for 220550 frames Cell Events: 6067 mean_circ: 0.951922
Ran for 221050 frames Cell Events: 6082 mean_circ: 0.951926
Ran for 221550 frames Cell Events: 6098 mean_circ: 0.951917
Ran for 222050 frames Cell Events: 6108 mean_circ: 0.951907
Ran for 222550 frames Cell Events: 6122 mean_circ: 0.951917
Ran for 223050 frames Cell Events: 6142 mean_circ: 0.951932
Ran for 223550 frames Cell Events: 6156 mean_circ: 0.95195
Ran for 224550 frames Cell Events: 6184 mean_circ: 0.951961
Ran for 225550 frames Cell Events: 6213 mean_circ: 0.951923
Ran for 226050 frames Cell Events: 6229 mean_circ: 0.95192
Ran for 226550 frames Cell Events: 6245 mean_circ: 0.951899
Ran for 229050 frames Cell Events: 6327 mean_circ: 0.951891
Ran for 229550 frames Cell Events: 6344 mean_circ: 0.951832
Ran for 230050 frames Cell Events: 6357 mean_circ: 0.951822
Ran for 230550 frames Cell Events: 6380 mean_circ: 0.951843
Ran for 231050 frames Cell Events: 6392 mean_circ: 0.951839
Ran for 231550 frames Cell Events: 6415 mean_circ: 0.951842
Ran for 232050 frames Cell Events: 6437 mean_circ: 0.951817
Ran for 232550 frames Cell Events: 6453 mean_circ: 0.951775
Ran for 233050 frames Cell Events: 6477 mean_circ: 0.951773
Ran for 233550 frames Cell Events: 6496 mean_circ: 0.951762
Ran for 235050 frames Cell Events: 6564 mean_circ: 0.951712
Ran for 235550 frames Cell Events: 6581 mean_circ: 0.951729
Ran for 236050 frames Cell Events: 6594 mean_circ: 0.951716
Ran for 236550 frames Cell Events: 6603 mean_circ: 0.951726
Ran for 237050 frames Cell Events: 6612 mean_circ: 0.951711
Ran for 237550 frames Cell Events: 6633 mean_circ: 0.951704
Ran for 238050 frames Cell Events: 6648 mean_circ: 0.951695
Ran for 238550 frames Cell Events: 6657 mean_circ: 0.95171
Ran for 239050 frames Cell Events: 6677 mean_circ: 0.951707
Ran for 239550 frames Cell Events: 6689 mean_circ: 0.951702
Ran for 240550 frames Cell Events: 6728 mean_circ: 0.951693
Ran for 241550 frames Cell Events: 6759 mean_circ: 0.951714
Ran for 242050 frames Cell Events: 6767 mean_circ: 0.951714
Ran for 242550 frames Cell Events: 6780 mean_circ: 0.951729
Ran for 243050 frames Cell Events: 6793 mean_circ: 0.951748
Ran for 243550 frames Cell Events: 6806 mean_circ: 0.951767
Ran for 244050 frames Cell Events: 6820 mean_circ: 0.951758
Ran for 244550 frames Cell Events: 6836 mean_circ: 0.951725
Ran for 245050 frames Cell Events: 6857 mean_circ: 0.951722
Ran for 245550 frames Cell Events: 6868 mean_circ: 0.951743
Ran for 246550 frames Cell Events: 6893 mean_circ: 0.951737
Ran for 247050 frames Cell Events: 6906 mean_circ: 0.951744
Ran for 247550 frames Cell Events: 6917 mean_circ: 0.951741
Ran for 248050 frames Cell Events: 6929 mean_circ: 0.951758
Ran for 248550 frames Cell Events: 6945 mean_circ: 0.951768
Ran for 250050 frames Cell Events: 6991 mean_circ: 0.951774
Ran for 251050 frames Cell Events: 7018 mean_circ: 0.951764
Ran for 252550 frames Cell Events: 7056 mean_circ: 0.9518
Ran for 253050 frames Cell Events: 7073 mean_circ: 0.951813
Ran for 253550 frames Cell Events: 7091 mean_circ: 0.951799
Ran for 254050 frames Cell Events: 7103 mean_circ: 0.951791
Ran for 255050 frames Cell Events: 7130 mean_circ: 0.951847
Ran for 256050 frames Cell Events: 7166 mean_circ: 0.951849
Ran for 256550 frames Cell Events: 7173 mean_circ: 0.951812
Ran for 257050 frames Cell Events: 7186 mean_circ: 0.951823
Ran for 258050 frames Cell Events: 7220 mean_circ: 0.951805
Ran for 259050 frames Cell Events: 7254 mean_circ: 0.951821
Ran for 259550 frames Cell Events: 7271 mean_circ: 0.951837
Ran for 260550 frames Cell Events: 7307 mean_circ: 0.951855
Ran for 261050 frames Cell Events: 7320 mean_circ: 0.951848
Ran for 261550 frames Cell Events: 7337 mean_circ: 0.951835
Ran for 262550 frames Cell Events: 7376 mean_circ: 0.951886
Ran for 263050 frames Cell Events: 7390 mean_circ: 0.951897
Ran for 263550 frames Cell Events: 7398 mean_circ: 0.951888
Ran for 264050 frames Cell Events: 7414 mean_circ: 0.951871
Ran for 265050 frames Cell Events: 7457 mean_circ: 0.951875
Ran for 265550 frames Cell Events: 7471 mean_circ: 0.951879
Ran for 266050 frames Cell Events: 7484 mean_circ: 0.951877
Ran for 266550 frames Cell Events: 7496 mean_circ: 0.951899
Ran for 267050 frames Cell Events: 7509 mean_circ: 0.951915
Ran for 267550 frames Cell Events: 7523 mean_circ: 0.951891
Ran for 268050 frames Cell Events: 7544 mean_circ: 0.951895
Ran for 268550 frames Cell Events: 7555 mean_circ: 0.951904
Ran for 269050 frames Cell Events: 7581 mean_circ: 0.951862
Ran for 269550 frames Cell Events: 7604 mean_circ: 0.951862
Ran for 270050 frames Cell Events: 7622 mean_circ: 0.951874
Ran for 271050 frames Cell Events: 7652 mean_circ: 0.951901
Ran for 271550 frames Cell Events: 7661 mean_circ: 0.951903
Ran for 272050 frames Cell Events: 7676 mean_circ: 0.951917
Ran for 272550 frames Cell Events: 7683 mean_circ: 0.951912
Ran for 273550 frames Cell Events: 7713 mean_circ: 0.951928
Ran for 274050 frames Cell Events: 7728 mean_circ: 0.951905
Ran for 274550 frames Cell Events: 7753 mean_circ: 0.951902
Ran for 275550 frames Cell Events: 7784 mean_circ: 0.951912
Ran for 276550 frames Cell Events: 7808 mean_circ: 0.951935
Ran for 277050 frames Cell Events: 7825 mean_circ: 0.951923
Ran for 277550 frames Cell Events: 7840 mean_circ: 0.951922
Ran for 278050 frames Cell Events: 7857 mean_circ: 0.951918
Ran for 278550 frames Cell Events: 7869 mean_circ: 0.951927
Ran for 279050 frames Cell Events: 7884 mean_circ: 0.951935
Ran for 280050 frames Cell Events: 7914 mean_circ: 0.951961
Ran for 280550 frames Cell Events: 7931 mean_circ: 0.95197
Ran for 281050 frames Cell Events: 7946 mean_circ: 0.952
Ran for 281550 frames Cell Events: 7962 mean_circ: 0.952016
Ran for 282050 frames Cell Events: 7972 mean_circ: 0.952016
Ran for 283050 frames Cell Events: 8012 mean_circ: 0.952042
Ran for 283550 frames Cell Events: 8027 mean_circ: 0.952053
Ran for 284050 frames Cell Events: 8047 mean_circ: 0.952069
Ran for 284550 frames Cell Events: 8066 mean_circ: 0.952077
Ran for 285050 frames Cell Events: 8081 mean_circ: 0.952091
Ran for 286550 frames Cell Events: 8137 mean_circ: 0.952089
Ran for 288050 frames Cell Events: 8181 mean_circ: 0.952113
Ran for 288550 frames Cell Events: 8194 mean_circ: 0.952119
Ran for 289050 frames Cell Events: 8210 mean_circ: 0.952108
Ran for 289550 frames Cell Events: 8223 mean_circ: 0.952105
Ran for 290050 frames Cell Events: 8237 mean_circ: 0.952117
Ran for 291050 frames Cell Events: 8270 mean_circ: 0.952074
Ran for 292050 frames Cell Events: 8313 mean_circ: 0.952102
Ran for 292550 frames Cell Events: 8331 mean_circ: 0.952092



End this measurment..



Stopping time: 2.36526e+009
****************************************
Measurement finished

Mean Circularitiy: 0.952089
Mean Area: 572.595
Mean FL-1 intensity: 3225.75
Mean FL-2 intensity: 3867.09
Mean FL-3 intensity: 0

frames processed: 292088
Total Time: 146807ms
Number of Runs (new bg img) 0
Frames collected: 293393
Skipped frames: 50258
Rescued Frames: 4458460
Samples DAQ: 73348371
Samples written: 146696546
Samples per Channel: 73348273
FPS: 1998.5

Samples per second: 499624
Expected samples/s: 500000

Events: 8354
Events per second: 56.9047
jumped back (PMT): 5151
#Frames read, frequency
# 0 frames :425213
# 1 frames :140977
# 2 frames :5247
# 3 frames :2430
# 4 frames :1408
# 5 frames :834
# 6 frames :846
# 7 frames :1934
# 8 frames :1342
# 9 frames :941
# 10 frames :746
# 11 frames :841
# 12 frames :701
# 13 frames :538
# 14 frames :372
# 15 frames :329
# 16 frames :276
# 17 frames :274
# 18 frames :290
# 19 frames :229
# 20 frames :158
# 21 frames :123
# 22 frames :92
# 23 frames :77
# 24 frames :77
# 25 frames :54
# 26 frames :44
# 27 frames :48
# 28 frames :40
# 29 frames :38
# 30 frames :41
# 31 frames :27
# 32 frames :29
# 33 frames :18
# 34 frames :14
# 35 frames :14
# 36 frames :13
# 37 frames :9
# 38 frames :2
# 39 frames :16
# 40 frames :4
# 41 frames :6
# 42 frames :4
# 43 frames :5
# 44 frames :3
# 45 frames :3
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 53 frames :3
# 54 frames :2
# 57 frames :3
# 60 frames :2
# 62 frames :1
# 67 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0269
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0