Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.16.12 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "afeca394362d5ef14b13b24ee7914999",
"name": "M1_20170611_1725_dsRNA_P8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:16:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8361 (max 8362) in '8361'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0336
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.36535e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 41 mean_circ: 0.928274
Ran for 2050 frames Cell Events: 85 mean_circ: 0.938736
Ran for 2550 frames Cell Events: 100 mean_circ: 0.940429
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Ran for 5050 frames Cell Events: 196 mean_circ: 0.942072
Ran for 5550 frames Cell Events: 214 mean_circ: 0.942006
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Ran for 6550 frames Cell Events: 247 mean_circ: 0.94197
Ran for 7550 frames Cell Events: 273 mean_circ: 0.94215
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Ran for 8550 frames Cell Events: 302 mean_circ: 0.94179
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Ran for 11550 frames Cell Events: 397 mean_circ: 0.94241
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Ran for 58550 frames Cell Events: 1922 mean_circ: 0.942188
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Ran for 76050 frames Cell Events: 2550 mean_circ: 0.942243
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Ran for 83550 frames Cell Events: 2830 mean_circ: 0.942212
Ran for 84050 frames Cell Events: 2846 mean_circ: 0.942242
Ran for 85550 frames Cell Events: 2892 mean_circ: 0.942294
Ran for 86550 frames Cell Events: 2938 mean_circ: 0.942246
Ran for 87050 frames Cell Events: 2953 mean_circ: 0.942232
Ran for 87550 frames Cell Events: 2982 mean_circ: 0.942295
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Ran for 92050 frames Cell Events: 3120 mean_circ: 0.942428
Ran for 94050 frames Cell Events: 3180 mean_circ: 0.942425
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Ran for 95050 frames Cell Events: 3212 mean_circ: 0.942364
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Ran for 96050 frames Cell Events: 3239 mean_circ: 0.942399
Ran for 96550 frames Cell Events: 3267 mean_circ: 0.942363
Ran for 97050 frames Cell Events: 3282 mean_circ: 0.942367
Ran for 98550 frames Cell Events: 3340 mean_circ: 0.942456
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Ran for 101050 frames Cell Events: 3436 mean_circ: 0.942599
Ran for 101550 frames Cell Events: 3444 mean_circ: 0.942629
Ran for 102050 frames Cell Events: 3459 mean_circ: 0.942612
Ran for 102550 frames Cell Events: 3475 mean_circ: 0.942629
Ran for 103050 frames Cell Events: 3496 mean_circ: 0.942668
Ran for 103550 frames Cell Events: 3511 mean_circ: 0.942555
Ran for 104050 frames Cell Events: 3523 mean_circ: 0.942573
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Ran for 106050 frames Cell Events: 3605 mean_circ: 0.942642
Ran for 106550 frames Cell Events: 3619 mean_circ: 0.942617
Ran for 107050 frames Cell Events: 3634 mean_circ: 0.942629
Ran for 107550 frames Cell Events: 3658 mean_circ: 0.942552
Ran for 108550 frames Cell Events: 3705 mean_circ: 0.942459
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Ran for 111050 frames Cell Events: 3789 mean_circ: 0.942408
Ran for 111550 frames Cell Events: 3806 mean_circ: 0.942448
Ran for 112050 frames Cell Events: 3822 mean_circ: 0.942424
Ran for 113550 frames Cell Events: 3889 mean_circ: 0.942375
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Ran for 117550 frames Cell Events: 3997 mean_circ: 0.94239
Ran for 118050 frames Cell Events: 4014 mean_circ: 0.942432
Ran for 120050 frames Cell Events: 4083 mean_circ: 0.942538
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Ran for 121550 frames Cell Events: 4140 mean_circ: 0.942368
Ran for 122550 frames Cell Events: 4179 mean_circ: 0.942377
Ran for 124050 frames Cell Events: 4227 mean_circ: 0.942336
Ran for 124550 frames Cell Events: 4244 mean_circ: 0.942313
Ran for 125550 frames Cell Events: 4282 mean_circ: 0.942195
Ran for 126050 frames Cell Events: 4301 mean_circ: 0.942165
Ran for 127050 frames Cell Events: 4346 mean_circ: 0.942086
Ran for 127550 frames Cell Events: 4361 mean_circ: 0.94203
Ran for 128050 frames Cell Events: 4371 mean_circ: 0.942042
Ran for 129550 frames Cell Events: 4416 mean_circ: 0.942087
Ran for 130550 frames Cell Events: 4445 mean_circ: 0.942058
Ran for 131550 frames Cell Events: 4474 mean_circ: 0.942057
Ran for 132050 frames Cell Events: 4496 mean_circ: 0.94194
Ran for 132550 frames Cell Events: 4509 mean_circ: 0.94186
Ran for 133050 frames Cell Events: 4520 mean_circ: 0.94186
Ran for 133550 frames Cell Events: 4538 mean_circ: 0.941807
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Ran for 141550 frames Cell Events: 4815 mean_circ: 0.941997
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Ran for 142550 frames Cell Events: 4856 mean_circ: 0.942028
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Ran for 173550 frames Cell Events: 5901 mean_circ: 0.941618
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Ran for 176550 frames Cell Events: 5994 mean_circ: 0.941606
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Ran for 178050 frames Cell Events: 6044 mean_circ: 0.94166
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Ran for 182550 frames Cell Events: 6212 mean_circ: 0.941635
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Ran for 197550 frames Cell Events: 6735 mean_circ: 0.94161
Ran for 198050 frames Cell Events: 6748 mean_circ: 0.941618
Ran for 198550 frames Cell Events: 6760 mean_circ: 0.941641
Ran for 199050 frames Cell Events: 6772 mean_circ: 0.941661
Ran for 199550 frames Cell Events: 6785 mean_circ: 0.941644
Ran for 202550 frames Cell Events: 6903 mean_circ: 0.941626
Ran for 203050 frames Cell Events: 6921 mean_circ: 0.941637
Ran for 206550 frames Cell Events: 7082 mean_circ: 0.941597
Ran for 207050 frames Cell Events: 7092 mean_circ: 0.941629
Ran for 207550 frames Cell Events: 7112 mean_circ: 0.941598
Ran for 208050 frames Cell Events: 7132 mean_circ: 0.94157
Ran for 208550 frames Cell Events: 7145 mean_circ: 0.941543
Ran for 209050 frames Cell Events: 7165 mean_circ: 0.941512
Ran for 210550 frames Cell Events: 7216 mean_circ: 0.941519
Ran for 211050 frames Cell Events: 7234 mean_circ: 0.941507
Ran for 213050 frames Cell Events: 7311 mean_circ: 0.941466
Ran for 213550 frames Cell Events: 7329 mean_circ: 0.941515
Ran for 214050 frames Cell Events: 7354 mean_circ: 0.941483
Ran for 214550 frames Cell Events: 7376 mean_circ: 0.94153
Ran for 215550 frames Cell Events: 7419 mean_circ: 0.941469
Ran for 216050 frames Cell Events: 7437 mean_circ: 0.941468
Ran for 216550 frames Cell Events: 7451 mean_circ: 0.941462
Ran for 217050 frames Cell Events: 7464 mean_circ: 0.94148
Ran for 217550 frames Cell Events: 7479 mean_circ: 0.941495
Ran for 218550 frames Cell Events: 7507 mean_circ: 0.941519
Ran for 220050 frames Cell Events: 7562 mean_circ: 0.941542
Ran for 221050 frames Cell Events: 7591 mean_circ: 0.941503
Ran for 222050 frames Cell Events: 7623 mean_circ: 0.941532
Ran for 222550 frames Cell Events: 7647 mean_circ: 0.94148
Ran for 224550 frames Cell Events: 7745 mean_circ: 0.941327
Ran for 225550 frames Cell Events: 7778 mean_circ: 0.941313
Ran for 226050 frames Cell Events: 7790 mean_circ: 0.94132
Ran for 226550 frames Cell Events: 7811 mean_circ: 0.94134
Ran for 227550 frames Cell Events: 7844 mean_circ: 0.941304
Ran for 228050 frames Cell Events: 7855 mean_circ: 0.941331
Ran for 228550 frames Cell Events: 7876 mean_circ: 0.941338
Ran for 229550 frames Cell Events: 7924 mean_circ: 0.941335
Ran for 230050 frames Cell Events: 7942 mean_circ: 0.941347
Ran for 231050 frames Cell Events: 7976 mean_circ: 0.941371
Ran for 233050 frames Cell Events: 8042 mean_circ: 0.941436
Ran for 233550 frames Cell Events: 8056 mean_circ: 0.941442
Ran for 234550 frames Cell Events: 8106 mean_circ: 0.941471
Ran for 235550 frames Cell Events: 8143 mean_circ: 0.941518
Ran for 236050 frames Cell Events: 8154 mean_circ: 0.941516
Ran for 236550 frames Cell Events: 8168 mean_circ: 0.941523
Ran for 237050 frames Cell Events: 8193 mean_circ: 0.941518
Ran for 237550 frames Cell Events: 8214 mean_circ: 0.941507
Ran for 238550 frames Cell Events: 8254 mean_circ: 0.941498
Ran for 239050 frames Cell Events: 8270 mean_circ: 0.941497
Ran for 239550 frames Cell Events: 8283 mean_circ: 0.94151
Ran for 240050 frames Cell Events: 8302 mean_circ: 0.941503
Ran for 240550 frames Cell Events: 8317 mean_circ: 0.941534
Ran for 241550 frames Cell Events: 8355 mean_circ: 0.941533



End this measurment..



Stopping time: 2.36547e+009
****************************************
Measurement finished

Mean Circularitiy: 0.941541
Mean Area: 574.553
Mean FL-1 intensity: 3090.46
Mean FL-2 intensity: 4255.2
Mean FL-3 intensity: 0

frames processed: 239325
Total Time: 121000ms
Number of Runs (new bg img) 0
Frames collected: 241765
Skipped frames: 52698
Rescued Frames: 4554414
Samples DAQ: 60441357
Samples written: 120882632
Samples per Channel: 60441316
FPS: 1998.07

Samples per second: 499517
Expected samples/s: 500000

Events: 8362
Events per second: 69.1077
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :340124
# 1 frames :125818
# 2 frames :4839
# 3 frames :1804
# 4 frames :966
# 5 frames :433
# 6 frames :2191
# 7 frames :1820
# 8 frames :1361
# 9 frames :757
# 10 frames :646
# 11 frames :468
# 12 frames :464
# 13 frames :318
# 14 frames :274
# 15 frames :207
# 16 frames :169
# 17 frames :171
# 18 frames :140
# 19 frames :109
# 20 frames :71
# 21 frames :67
# 22 frames :36
# 23 frames :34
# 24 frames :44
# 25 frames :27
# 26 frames :27
# 27 frames :12
# 28 frames :15
# 29 frames :18
# 30 frames :12
# 31 frames :8
# 32 frames :5
# 33 frames :5
# 34 frames :6
# 35 frames :4
# 36 frames :5
# 37 frames :6
# 38 frames :3
# 39 frames :3
# 40 frames :1
# 41 frames :2
# 42 frames :2
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 51 frames :2
# 53 frames :1
# 55 frames :1
# 59 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0336
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0