Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.19.34 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e4388d8e2bc8ae36e99d5222abd9f815",
"name": "M1_20170611_1814_dsRNA_P7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:19:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8395 (max 8396) in '8395'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1097
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.3683e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 94 mean_circ: 0.940533
Ran for 2550 frames Cell Events: 116 mean_circ: 0.941801
Ran for 3050 frames Cell Events: 143 mean_circ: 0.941012
Ran for 4050 frames Cell Events: 189 mean_circ: 0.940191
Ran for 5550 frames Cell Events: 265 mean_circ: 0.942305
Ran for 6050 frames Cell Events: 284 mean_circ: 0.941195
Ran for 7050 frames Cell Events: 315 mean_circ: 0.94208
Ran for 7550 frames Cell Events: 339 mean_circ: 0.941795
Ran for 8050 frames Cell Events: 363 mean_circ: 0.940887
Ran for 8550 frames Cell Events: 378 mean_circ: 0.940284
Ran for 9050 frames Cell Events: 404 mean_circ: 0.940663
Ran for 9550 frames Cell Events: 434 mean_circ: 0.941487
Ran for 10550 frames Cell Events: 499 mean_circ: 0.941702
Ran for 11050 frames Cell Events: 527 mean_circ: 0.941507
Ran for 11550 frames Cell Events: 552 mean_circ: 0.941827
Ran for 12550 frames Cell Events: 609 mean_circ: 0.941958
Ran for 13050 frames Cell Events: 622 mean_circ: 0.941793
Ran for 14050 frames Cell Events: 674 mean_circ: 0.941801
Ran for 14550 frames Cell Events: 698 mean_circ: 0.942305
Ran for 17050 frames Cell Events: 824 mean_circ: 0.941172
Ran for 17550 frames Cell Events: 844 mean_circ: 0.941548
Ran for 18050 frames Cell Events: 855 mean_circ: 0.941538
Ran for 18550 frames Cell Events: 869 mean_circ: 0.941427
Ran for 19550 frames Cell Events: 902 mean_circ: 0.941635
Ran for 21050 frames Cell Events: 1002 mean_circ: 0.941159
Ran for 22550 frames Cell Events: 1073 mean_circ: 0.941503
Ran for 23050 frames Cell Events: 1098 mean_circ: 0.941517
Ran for 24050 frames Cell Events: 1147 mean_circ: 0.941721
Ran for 26050 frames Cell Events: 1235 mean_circ: 0.941418
Ran for 26550 frames Cell Events: 1258 mean_circ: 0.941477
Ran for 27550 frames Cell Events: 1293 mean_circ: 0.941356
Ran for 28550 frames Cell Events: 1334 mean_circ: 0.941158
Ran for 29050 frames Cell Events: 1365 mean_circ: 0.94118
Ran for 29550 frames Cell Events: 1382 mean_circ: 0.941176
Ran for 30550 frames Cell Events: 1426 mean_circ: 0.941024
Ran for 31550 frames Cell Events: 1470 mean_circ: 0.94096
Ran for 33550 frames Cell Events: 1557 mean_circ: 0.940454
Ran for 34050 frames Cell Events: 1590 mean_circ: 0.94037
Ran for 35550 frames Cell Events: 1641 mean_circ: 0.940708
Ran for 39050 frames Cell Events: 1831 mean_circ: 0.941165
Ran for 40550 frames Cell Events: 1916 mean_circ: 0.941215
Ran for 41550 frames Cell Events: 1962 mean_circ: 0.940892
Ran for 45050 frames Cell Events: 2137 mean_circ: 0.940617
Ran for 45550 frames Cell Events: 2168 mean_circ: 0.940595
Ran for 47550 frames Cell Events: 2290 mean_circ: 0.940636
Ran for 48050 frames Cell Events: 2307 mean_circ: 0.94058
Ran for 48550 frames Cell Events: 2333 mean_circ: 0.940646
Ran for 49050 frames Cell Events: 2349 mean_circ: 0.940672
Ran for 50550 frames Cell Events: 2440 mean_circ: 0.94061
Ran for 51050 frames Cell Events: 2467 mean_circ: 0.94042
Ran for 52050 frames Cell Events: 2512 mean_circ: 0.940394
Ran for 53050 frames Cell Events: 2558 mean_circ: 0.940394
Ran for 53550 frames Cell Events: 2583 mean_circ: 0.940425
Ran for 54050 frames Cell Events: 2614 mean_circ: 0.940295
Ran for 56550 frames Cell Events: 2752 mean_circ: 0.940274
Ran for 57550 frames Cell Events: 2802 mean_circ: 0.940433
Ran for 58550 frames Cell Events: 2854 mean_circ: 0.940518
Ran for 59050 frames Cell Events: 2884 mean_circ: 0.940572
Ran for 60050 frames Cell Events: 2944 mean_circ: 0.940611
Ran for 62550 frames Cell Events: 3084 mean_circ: 0.94026
Ran for 63050 frames Cell Events: 3103 mean_circ: 0.940257
Ran for 63550 frames Cell Events: 3128 mean_circ: 0.940226
Ran for 64050 frames Cell Events: 3151 mean_circ: 0.940308
Ran for 67550 frames Cell Events: 3350 mean_circ: 0.940416
Ran for 68050 frames Cell Events: 3375 mean_circ: 0.940318
Ran for 69050 frames Cell Events: 3417 mean_circ: 0.940383
Ran for 70550 frames Cell Events: 3495 mean_circ: 0.940277
Ran for 71550 frames Cell Events: 3551 mean_circ: 0.940314
Ran for 72050 frames Cell Events: 3573 mean_circ: 0.940292
Ran for 73550 frames Cell Events: 3663 mean_circ: 0.940225
Ran for 74050 frames Cell Events: 3682 mean_circ: 0.940284
Ran for 74550 frames Cell Events: 3701 mean_circ: 0.940297
Ran for 76050 frames Cell Events: 3772 mean_circ: 0.940211
Ran for 76550 frames Cell Events: 3792 mean_circ: 0.940157
Ran for 77550 frames Cell Events: 3842 mean_circ: 0.940215
Ran for 80550 frames Cell Events: 3976 mean_circ: 0.940302
Ran for 82050 frames Cell Events: 4056 mean_circ: 0.940345
Ran for 82550 frames Cell Events: 4084 mean_circ: 0.940267
Ran for 84050 frames Cell Events: 4173 mean_circ: 0.940299
Ran for 86050 frames Cell Events: 4293 mean_circ: 0.94026
Ran for 87050 frames Cell Events: 4347 mean_circ: 0.940094
Ran for 88550 frames Cell Events: 4413 mean_circ: 0.940086
Ran for 90050 frames Cell Events: 4467 mean_circ: 0.940001
Ran for 91050 frames Cell Events: 4513 mean_circ: 0.940019
Ran for 91550 frames Cell Events: 4542 mean_circ: 0.940022
Ran for 92050 frames Cell Events: 4570 mean_circ: 0.940025
Ran for 93050 frames Cell Events: 4621 mean_circ: 0.939975
Ran for 93550 frames Cell Events: 4631 mean_circ: 0.939989
Ran for 94050 frames Cell Events: 4653 mean_circ: 0.940007
Ran for 95050 frames Cell Events: 4707 mean_circ: 0.94007
Ran for 95550 frames Cell Events: 4725 mean_circ: 0.940024
Ran for 96050 frames Cell Events: 4747 mean_circ: 0.940063
Ran for 96550 frames Cell Events: 4764 mean_circ: 0.940019
Ran for 97050 frames Cell Events: 4788 mean_circ: 0.939978
Ran for 97550 frames Cell Events: 4808 mean_circ: 0.939993
Ran for 99550 frames Cell Events: 4901 mean_circ: 0.940087
Ran for 100050 frames Cell Events: 4919 mean_circ: 0.940089
Ran for 101050 frames Cell Events: 4967 mean_circ: 0.940181
Ran for 104050 frames Cell Events: 5146 mean_circ: 0.940298
Ran for 105050 frames Cell Events: 5197 mean_circ: 0.940325
Ran for 105550 frames Cell Events: 5227 mean_circ: 0.94031
Ran for 106050 frames Cell Events: 5249 mean_circ: 0.940312
Ran for 106550 frames Cell Events: 5275 mean_circ: 0.940305
Ran for 107050 frames Cell Events: 5288 mean_circ: 0.940396
Ran for 107550 frames Cell Events: 5310 mean_circ: 0.940321
Ran for 109050 frames Cell Events: 5395 mean_circ: 0.940283
Ran for 109550 frames Cell Events: 5422 mean_circ: 0.94027
Ran for 110050 frames Cell Events: 5444 mean_circ: 0.940271
Ran for 110550 frames Cell Events: 5468 mean_circ: 0.940244
Ran for 111050 frames Cell Events: 5485 mean_circ: 0.940238
Ran for 113550 frames Cell Events: 5594 mean_circ: 0.940149
Ran for 114550 frames Cell Events: 5637 mean_circ: 0.94005
Ran for 115050 frames Cell Events: 5660 mean_circ: 0.94005
Ran for 115550 frames Cell Events: 5684 mean_circ: 0.939971
Ran for 116550 frames Cell Events: 5731 mean_circ: 0.939836
Ran for 117550 frames Cell Events: 5791 mean_circ: 0.939799
Ran for 118050 frames Cell Events: 5809 mean_circ: 0.939805
Ran for 119050 frames Cell Events: 5859 mean_circ: 0.939818
Ran for 120050 frames Cell Events: 5907 mean_circ: 0.939802
Ran for 120550 frames Cell Events: 5920 mean_circ: 0.93975
Ran for 121050 frames Cell Events: 5940 mean_circ: 0.939755
Ran for 121550 frames Cell Events: 5963 mean_circ: 0.939775
Ran for 122050 frames Cell Events: 5990 mean_circ: 0.939839
Ran for 122550 frames Cell Events: 6016 mean_circ: 0.939855
Ran for 123050 frames Cell Events: 6034 mean_circ: 0.939833
Ran for 124050 frames Cell Events: 6093 mean_circ: 0.939792
Ran for 125550 frames Cell Events: 6162 mean_circ: 0.939878
Ran for 126550 frames Cell Events: 6201 mean_circ: 0.939894
Ran for 127050 frames Cell Events: 6224 mean_circ: 0.939891
Ran for 128050 frames Cell Events: 6249 mean_circ: 0.939878
Ran for 130550 frames Cell Events: 6358 mean_circ: 0.939906
Ran for 133550 frames Cell Events: 6515 mean_circ: 0.93977
Ran for 135550 frames Cell Events: 6600 mean_circ: 0.939811
Ran for 136550 frames Cell Events: 6654 mean_circ: 0.939777
Ran for 139050 frames Cell Events: 6781 mean_circ: 0.939884
Ran for 139550 frames Cell Events: 6800 mean_circ: 0.939865
Ran for 141050 frames Cell Events: 6874 mean_circ: 0.939881
Ran for 142050 frames Cell Events: 6920 mean_circ: 0.93995
Ran for 142550 frames Cell Events: 6949 mean_circ: 0.93998
Ran for 143050 frames Cell Events: 6967 mean_circ: 0.939995
Ran for 145550 frames Cell Events: 7107 mean_circ: 0.939987
Ran for 146050 frames Cell Events: 7141 mean_circ: 0.939974
Ran for 148550 frames Cell Events: 7271 mean_circ: 0.939848
Ran for 149550 frames Cell Events: 7330 mean_circ: 0.939862
Ran for 150050 frames Cell Events: 7365 mean_circ: 0.939913
Ran for 150550 frames Cell Events: 7390 mean_circ: 0.93989
Ran for 151550 frames Cell Events: 7440 mean_circ: 0.93999
Ran for 152050 frames Cell Events: 7457 mean_circ: 0.93996
Ran for 152550 frames Cell Events: 7487 mean_circ: 0.939963
Ran for 153550 frames Cell Events: 7534 mean_circ: 0.939971
Ran for 154550 frames Cell Events: 7570 mean_circ: 0.939892
Ran for 155550 frames Cell Events: 7623 mean_circ: 0.939896
Ran for 156050 frames Cell Events: 7644 mean_circ: 0.939952
Ran for 160550 frames Cell Events: 7904 mean_circ: 0.939928
Ran for 163050 frames Cell Events: 8042 mean_circ: 0.939885
Ran for 163550 frames Cell Events: 8071 mean_circ: 0.939912
Ran for 164050 frames Cell Events: 8102 mean_circ: 0.939887
Ran for 166550 frames Cell Events: 8244 mean_circ: 0.939877
Ran for 167050 frames Cell Events: 8273 mean_circ: 0.939905
Ran for 167550 frames Cell Events: 8296 mean_circ: 0.939875
Ran for 168050 frames Cell Events: 8335 mean_circ: 0.939875



End this measurment..



Stopping time: 2.36838e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93989
Mean Area: 594.095
Mean FL-1 intensity: 3089.67
Mean FL-2 intensity: 4377.47
Mean FL-3 intensity: 0

frames processed: 167590
Total Time: 84566.4ms
Number of Runs (new bg img) 0
Frames collected: 169017
Skipped frames: 58596
Rescued Frames: 5473417
Samples DAQ: 42254862
Samples written: 84508320
Samples per Channel: 42254160
FPS: 1998.63

Samples per second: 499657
Expected samples/s: 500000

Events: 8396
Events per second: 99.283
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :266747
# 1 frames :57531
# 2 frames :3245
# 3 frames :1169
# 4 frames :809
# 5 frames :427
# 6 frames :1064
# 7 frames :1315
# 8 frames :987
# 9 frames :711
# 10 frames :513
# 11 frames :510
# 12 frames :426
# 13 frames :313
# 14 frames :260
# 15 frames :264
# 16 frames :253
# 17 frames :187
# 18 frames :177
# 19 frames :150
# 20 frames :119
# 21 frames :93
# 22 frames :74
# 23 frames :87
# 24 frames :81
# 25 frames :60
# 26 frames :45
# 27 frames :45
# 28 frames :34
# 29 frames :31
# 30 frames :28
# 31 frames :33
# 32 frames :21
# 33 frames :21
# 34 frames :18
# 35 frames :14
# 36 frames :17
# 37 frames :13
# 38 frames :7
# 39 frames :13
# 40 frames :6
# 41 frames :5
# 42 frames :6
# 43 frames :1
# 44 frames :7
# 45 frames :3
# 46 frames :4
# 47 frames :6
# 48 frames :5
# 49 frames :4
# 50 frames :2
# 51 frames :2
# 52 frames :2
# 53 frames :2
# 55 frames :2
# 56 frames :3
# 59 frames :1
# 61 frames :1
# 62 frames :1
# 67 frames :3
# 69 frames :2
# 71 frames :1
# 72 frames :1
# 83 frames :1
# 90 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1097
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0