Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.20.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3f9573b037d5eb182b2858d0ab36c3a0",
"name": "M1_20170611_1825_dsRNA_Q3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:20:51"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8609 (max 8610) in '8609'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1127
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.36892e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.950265
Ran for 1050 frames Cell Events: 37 mean_circ: 0.948194
Ran for 1550 frames Cell Events: 60 mean_circ: 0.94735
Ran for 2050 frames Cell Events: 79 mean_circ: 0.9435
Ran for 3050 frames Cell Events: 118 mean_circ: 0.944277
Ran for 4050 frames Cell Events: 163 mean_circ: 0.944158
Ran for 5550 frames Cell Events: 225 mean_circ: 0.941437
Ran for 6050 frames Cell Events: 246 mean_circ: 0.941029
Ran for 7550 frames Cell Events: 311 mean_circ: 0.94154
Ran for 8050 frames Cell Events: 327 mean_circ: 0.941706
Ran for 8550 frames Cell Events: 341 mean_circ: 0.941856
Ran for 9050 frames Cell Events: 363 mean_circ: 0.942458
Ran for 10050 frames Cell Events: 407 mean_circ: 0.942171
Ran for 11050 frames Cell Events: 444 mean_circ: 0.942731
Ran for 11550 frames Cell Events: 462 mean_circ: 0.942026
Ran for 12050 frames Cell Events: 477 mean_circ: 0.942188
Ran for 12550 frames Cell Events: 496 mean_circ: 0.942087
Ran for 13050 frames Cell Events: 502 mean_circ: 0.941846
Ran for 14050 frames Cell Events: 561 mean_circ: 0.940062
Ran for 14550 frames Cell Events: 586 mean_circ: 0.939988
Ran for 16050 frames Cell Events: 660 mean_circ: 0.941467
Ran for 16550 frames Cell Events: 674 mean_circ: 0.941677
Ran for 17050 frames Cell Events: 693 mean_circ: 0.941517
Ran for 17550 frames Cell Events: 722 mean_circ: 0.941498
Ran for 18550 frames Cell Events: 761 mean_circ: 0.941
Ran for 20050 frames Cell Events: 825 mean_circ: 0.940742
Ran for 20550 frames Cell Events: 848 mean_circ: 0.940678
Ran for 21050 frames Cell Events: 867 mean_circ: 0.940825
Ran for 22550 frames Cell Events: 938 mean_circ: 0.940924
Ran for 23050 frames Cell Events: 957 mean_circ: 0.941075
Ran for 23550 frames Cell Events: 982 mean_circ: 0.940952
Ran for 25050 frames Cell Events: 1055 mean_circ: 0.940634
Ran for 25550 frames Cell Events: 1076 mean_circ: 0.940364
Ran for 26550 frames Cell Events: 1109 mean_circ: 0.940419
Ran for 27550 frames Cell Events: 1158 mean_circ: 0.940329
Ran for 28550 frames Cell Events: 1207 mean_circ: 0.940439
Ran for 29050 frames Cell Events: 1232 mean_circ: 0.940642
Ran for 29550 frames Cell Events: 1247 mean_circ: 0.940598
Ran for 31550 frames Cell Events: 1350 mean_circ: 0.940914
Ran for 32050 frames Cell Events: 1375 mean_circ: 0.940969
Ran for 33050 frames Cell Events: 1415 mean_circ: 0.940915
Ran for 33550 frames Cell Events: 1432 mean_circ: 0.940782
Ran for 34050 frames Cell Events: 1456 mean_circ: 0.940963
Ran for 35050 frames Cell Events: 1509 mean_circ: 0.940819
Ran for 36050 frames Cell Events: 1576 mean_circ: 0.940191
Ran for 36550 frames Cell Events: 1603 mean_circ: 0.940025
Ran for 38050 frames Cell Events: 1669 mean_circ: 0.939956
Ran for 39050 frames Cell Events: 1704 mean_circ: 0.939821
Ran for 39550 frames Cell Events: 1726 mean_circ: 0.939916
Ran for 42050 frames Cell Events: 1846 mean_circ: 0.940066
Ran for 42550 frames Cell Events: 1868 mean_circ: 0.940009
Ran for 43050 frames Cell Events: 1886 mean_circ: 0.93996
Ran for 43550 frames Cell Events: 1904 mean_circ: 0.93985
Ran for 44050 frames Cell Events: 1926 mean_circ: 0.939792
Ran for 44550 frames Cell Events: 1939 mean_circ: 0.939943
Ran for 46550 frames Cell Events: 2035 mean_circ: 0.939909
Ran for 47050 frames Cell Events: 2056 mean_circ: 0.939983
Ran for 48550 frames Cell Events: 2131 mean_circ: 0.939933
Ran for 49550 frames Cell Events: 2189 mean_circ: 0.940089
Ran for 50550 frames Cell Events: 2243 mean_circ: 0.940259
Ran for 51550 frames Cell Events: 2301 mean_circ: 0.940392
Ran for 52050 frames Cell Events: 2324 mean_circ: 0.940314
Ran for 53050 frames Cell Events: 2379 mean_circ: 0.940299
Ran for 53550 frames Cell Events: 2405 mean_circ: 0.940278
Ran for 54050 frames Cell Events: 2429 mean_circ: 0.940126
Ran for 55550 frames Cell Events: 2521 mean_circ: 0.940223
Ran for 56050 frames Cell Events: 2539 mean_circ: 0.940509
Ran for 56550 frames Cell Events: 2559 mean_circ: 0.94051
Ran for 57550 frames Cell Events: 2602 mean_circ: 0.940601
Ran for 59550 frames Cell Events: 2694 mean_circ: 0.940819
Ran for 60050 frames Cell Events: 2719 mean_circ: 0.940815
Ran for 61050 frames Cell Events: 2759 mean_circ: 0.940847
Ran for 63050 frames Cell Events: 2849 mean_circ: 0.940992
Ran for 63550 frames Cell Events: 2872 mean_circ: 0.940911
Ran for 64550 frames Cell Events: 2907 mean_circ: 0.940834
Ran for 65050 frames Cell Events: 2929 mean_circ: 0.940886
Ran for 65550 frames Cell Events: 2945 mean_circ: 0.940864
Ran for 66050 frames Cell Events: 2964 mean_circ: 0.940786
Ran for 67050 frames Cell Events: 3004 mean_circ: 0.94069
Ran for 67550 frames Cell Events: 3023 mean_circ: 0.940669
Ran for 69050 frames Cell Events: 3101 mean_circ: 0.940629
Ran for 70050 frames Cell Events: 3145 mean_circ: 0.940556
Ran for 71050 frames Cell Events: 3184 mean_circ: 0.940587
Ran for 71550 frames Cell Events: 3195 mean_circ: 0.940533
Ran for 72050 frames Cell Events: 3215 mean_circ: 0.940529
Ran for 72550 frames Cell Events: 3233 mean_circ: 0.940543
Ran for 73550 frames Cell Events: 3274 mean_circ: 0.940599
Ran for 74050 frames Cell Events: 3289 mean_circ: 0.940615
Ran for 75050 frames Cell Events: 3346 mean_circ: 0.940611
Ran for 77050 frames Cell Events: 3430 mean_circ: 0.940594
Ran for 78550 frames Cell Events: 3491 mean_circ: 0.940507
Ran for 79050 frames Cell Events: 3501 mean_circ: 0.940538
Ran for 79550 frames Cell Events: 3523 mean_circ: 0.940487
Ran for 80550 frames Cell Events: 3561 mean_circ: 0.940508
Ran for 81550 frames Cell Events: 3609 mean_circ: 0.940579
Ran for 82050 frames Cell Events: 3637 mean_circ: 0.940639
Ran for 82550 frames Cell Events: 3657 mean_circ: 0.94072
Ran for 83550 frames Cell Events: 3697 mean_circ: 0.940823
Ran for 84050 frames Cell Events: 3709 mean_circ: 0.940777
Ran for 84550 frames Cell Events: 3729 mean_circ: 0.940808
Ran for 85050 frames Cell Events: 3745 mean_circ: 0.94085
Ran for 85550 frames Cell Events: 3774 mean_circ: 0.940831
Ran for 86050 frames Cell Events: 3796 mean_circ: 0.940688
Ran for 87050 frames Cell Events: 3837 mean_circ: 0.940624
Ran for 87550 frames Cell Events: 3849 mean_circ: 0.940636
Ran for 88550 frames Cell Events: 3906 mean_circ: 0.940595
Ran for 90550 frames Cell Events: 4005 mean_circ: 0.940595
Ran for 91050 frames Cell Events: 4028 mean_circ: 0.94061
Ran for 91550 frames Cell Events: 4047 mean_circ: 0.940624
Ran for 92550 frames Cell Events: 4091 mean_circ: 0.94053
Ran for 93050 frames Cell Events: 4114 mean_circ: 0.940521
Ran for 94550 frames Cell Events: 4183 mean_circ: 0.940573
Ran for 95550 frames Cell Events: 4228 mean_circ: 0.940511
Ran for 96050 frames Cell Events: 4247 mean_circ: 0.940528
Ran for 96550 frames Cell Events: 4268 mean_circ: 0.940478
Ran for 99550 frames Cell Events: 4479 mean_circ: 0.940283
Ran for 100050 frames Cell Events: 4507 mean_circ: 0.94021
Ran for 103550 frames Cell Events: 4688 mean_circ: 0.940296
Ran for 104050 frames Cell Events: 4720 mean_circ: 0.940327
Ran for 105050 frames Cell Events: 4768 mean_circ: 0.940289
Ran for 105550 frames Cell Events: 4790 mean_circ: 0.940378
Ran for 106550 frames Cell Events: 4819 mean_circ: 0.940309
Ran for 108050 frames Cell Events: 4889 mean_circ: 0.940377
Ran for 109050 frames Cell Events: 4922 mean_circ: 0.940336
Ran for 109550 frames Cell Events: 4950 mean_circ: 0.940375
Ran for 111050 frames Cell Events: 5030 mean_circ: 0.940269
Ran for 111550 frames Cell Events: 5059 mean_circ: 0.940293
Ran for 112050 frames Cell Events: 5096 mean_circ: 0.940367
Ran for 113050 frames Cell Events: 5155 mean_circ: 0.940363
Ran for 114050 frames Cell Events: 5209 mean_circ: 0.940348
Ran for 114550 frames Cell Events: 5235 mean_circ: 0.940418
Ran for 115550 frames Cell Events: 5289 mean_circ: 0.940467
Ran for 116550 frames Cell Events: 5336 mean_circ: 0.940391
Ran for 117050 frames Cell Events: 5358 mean_circ: 0.940368
Ran for 118050 frames Cell Events: 5410 mean_circ: 0.940409
Ran for 118550 frames Cell Events: 5429 mean_circ: 0.940426
Ran for 119550 frames Cell Events: 5473 mean_circ: 0.940483
Ran for 120050 frames Cell Events: 5495 mean_circ: 0.940465
Ran for 120550 frames Cell Events: 5515 mean_circ: 0.940485
Ran for 121550 frames Cell Events: 5557 mean_circ: 0.940448
Ran for 122550 frames Cell Events: 5600 mean_circ: 0.94052
Ran for 123050 frames Cell Events: 5616 mean_circ: 0.940495
Ran for 123550 frames Cell Events: 5636 mean_circ: 0.940445
Ran for 124050 frames Cell Events: 5649 mean_circ: 0.940406
Ran for 124550 frames Cell Events: 5674 mean_circ: 0.940458
Ran for 125050 frames Cell Events: 5692 mean_circ: 0.940434
Ran for 127050 frames Cell Events: 5797 mean_circ: 0.940378
Ran for 127550 frames Cell Events: 5817 mean_circ: 0.940405
Ran for 128550 frames Cell Events: 5860 mean_circ: 0.940406
Ran for 129550 frames Cell Events: 5906 mean_circ: 0.940464
Ran for 130550 frames Cell Events: 5961 mean_circ: 0.940502
Ran for 131550 frames Cell Events: 6001 mean_circ: 0.940542
Ran for 133050 frames Cell Events: 6059 mean_circ: 0.940511
Ran for 133550 frames Cell Events: 6081 mean_circ: 0.94053
Ran for 134050 frames Cell Events: 6107 mean_circ: 0.940504
Ran for 135050 frames Cell Events: 6155 mean_circ: 0.940443
Ran for 135550 frames Cell Events: 6181 mean_circ: 0.940467
Ran for 136550 frames Cell Events: 6229 mean_circ: 0.9405
Ran for 137050 frames Cell Events: 6254 mean_circ: 0.940467
Ran for 137550 frames Cell Events: 6270 mean_circ: 0.940491
Ran for 139050 frames Cell Events: 6332 mean_circ: 0.940555
Ran for 140050 frames Cell Events: 6368 mean_circ: 0.940556
Ran for 140550 frames Cell Events: 6389 mean_circ: 0.940544
Ran for 141550 frames Cell Events: 6435 mean_circ: 0.940498
Ran for 142050 frames Cell Events: 6461 mean_circ: 0.940507
Ran for 142550 frames Cell Events: 6485 mean_circ: 0.940534
Ran for 143050 frames Cell Events: 6505 mean_circ: 0.940536
Ran for 144050 frames Cell Events: 6571 mean_circ: 0.940484
Ran for 145050 frames Cell Events: 6613 mean_circ: 0.94047
Ran for 147050 frames Cell Events: 6688 mean_circ: 0.940386
Ran for 147550 frames Cell Events: 6714 mean_circ: 0.9404
Ran for 148050 frames Cell Events: 6729 mean_circ: 0.94037
Ran for 148550 frames Cell Events: 6752 mean_circ: 0.940374
Ran for 149050 frames Cell Events: 6767 mean_circ: 0.940387
Ran for 149550 frames Cell Events: 6790 mean_circ: 0.94038
Ran for 150050 frames Cell Events: 6814 mean_circ: 0.940404
Ran for 152550 frames Cell Events: 6926 mean_circ: 0.940453
Ran for 153050 frames Cell Events: 6951 mean_circ: 0.940404
Ran for 154050 frames Cell Events: 7006 mean_circ: 0.940389
Ran for 154550 frames Cell Events: 7025 mean_circ: 0.940368
Ran for 155050 frames Cell Events: 7052 mean_circ: 0.940328
Ran for 156050 frames Cell Events: 7107 mean_circ: 0.940376
Ran for 159050 frames Cell Events: 7233 mean_circ: 0.940333
Ran for 161050 frames Cell Events: 7321 mean_circ: 0.940397
Ran for 161550 frames Cell Events: 7345 mean_circ: 0.940393
Ran for 162550 frames Cell Events: 7390 mean_circ: 0.940363
Ran for 163050 frames Cell Events: 7411 mean_circ: 0.940386
Ran for 163550 frames Cell Events: 7440 mean_circ: 0.940342
Ran for 164050 frames Cell Events: 7457 mean_circ: 0.940372
Ran for 165050 frames Cell Events: 7509 mean_circ: 0.940407
Ran for 166550 frames Cell Events: 7568 mean_circ: 0.940357
Ran for 168050 frames Cell Events: 7642 mean_circ: 0.940328
Ran for 168550 frames Cell Events: 7661 mean_circ: 0.94036
Ran for 171050 frames Cell Events: 7762 mean_circ: 0.940362
Ran for 172050 frames Cell Events: 7802 mean_circ: 0.940354
Ran for 172550 frames Cell Events: 7818 mean_circ: 0.940309
Ran for 174550 frames Cell Events: 7903 mean_circ: 0.940255
Ran for 175050 frames Cell Events: 7935 mean_circ: 0.940229
Ran for 175550 frames Cell Events: 7949 mean_circ: 0.940253
Ran for 176050 frames Cell Events: 7969 mean_circ: 0.940272
Ran for 176550 frames Cell Events: 7993 mean_circ: 0.9403
Ran for 178050 frames Cell Events: 8060 mean_circ: 0.94032
Ran for 180050 frames Cell Events: 8147 mean_circ: 0.940343
Ran for 181550 frames Cell Events: 8203 mean_circ: 0.940338
Ran for 182050 frames Cell Events: 8225 mean_circ: 0.940347
Ran for 182550 frames Cell Events: 8247 mean_circ: 0.94033
Ran for 183050 frames Cell Events: 8265 mean_circ: 0.940348
Ran for 185050 frames Cell Events: 8365 mean_circ: 0.940357
Ran for 185550 frames Cell Events: 8389 mean_circ: 0.940358
Ran for 187050 frames Cell Events: 8480 mean_circ: 0.940386
Ran for 187550 frames Cell Events: 8501 mean_circ: 0.940406
Ran for 188050 frames Cell Events: 8522 mean_circ: 0.940408
Ran for 188550 frames Cell Events: 8539 mean_circ: 0.940426



End this measurment..



Stopping time: 2.36901e+009
****************************************
Measurement finished

Mean Circularitiy: 0.940415
Mean Area: 586.908
Mean FL-1 intensity: 3062.86
Mean FL-2 intensity: 4274.31
Mean FL-3 intensity: 0

frames processed: 189195
Total Time: 95157.2ms
Number of Runs (new bg img) 0
Frames collected: 190122
Skipped frames: 59761
Rescued Frames: 5671990
Samples DAQ: 47530843
Samples written: 95061054
Samples per Channel: 47530527
FPS: 1997.98

Samples per second: 499495
Expected samples/s: 500000

Events: 8610
Events per second: 90.4818
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :291943
# 1 frames :71542
# 2 frames :3888
# 3 frames :1447
# 4 frames :933
# 5 frames :454
# 6 frames :1346
# 7 frames :1458
# 8 frames :1111
# 9 frames :666
# 10 frames :735
# 11 frames :549
# 12 frames :476
# 13 frames :324
# 14 frames :280
# 15 frames :278
# 16 frames :271
# 17 frames :219
# 18 frames :194
# 19 frames :145
# 20 frames :116
# 21 frames :88
# 22 frames :67
# 23 frames :62
# 24 frames :51
# 25 frames :51
# 26 frames :41
# 27 frames :40
# 28 frames :24
# 29 frames :24
# 30 frames :28
# 31 frames :30
# 32 frames :15
# 33 frames :10
# 34 frames :21
# 35 frames :15
# 36 frames :20
# 37 frames :8
# 38 frames :6
# 39 frames :14
# 40 frames :7
# 41 frames :6
# 42 frames :5
# 43 frames :4
# 44 frames :6
# 45 frames :4
# 46 frames :2
# 47 frames :4
# 48 frames :4
# 49 frames :3
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :2
# 55 frames :1
# 56 frames :1
# 58 frames :1
# 62 frames :2
# 63 frames :1
# 66 frames :1
# 73 frames :1
# 79 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1127
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0