Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.40.07 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "eaf9fc4bf21c8676c6c0853d7fcd6c3f",
"name": "M1_20170612_1535_dsRNA_R6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:40:27"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8040 (max 8041) in '8040'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7825
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44512e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.948567
Ran for 1050 frames Cell Events: 39 mean_circ: 0.948662
Ran for 1550 frames Cell Events: 47 mean_circ: 0.949061
Ran for 2550 frames Cell Events: 78 mean_circ: 0.947177
Ran for 3550 frames Cell Events: 116 mean_circ: 0.945595
Ran for 4050 frames Cell Events: 137 mean_circ: 0.945879
Ran for 4550 frames Cell Events: 154 mean_circ: 0.946578
Ran for 5050 frames Cell Events: 176 mean_circ: 0.946578
Ran for 5550 frames Cell Events: 197 mean_circ: 0.945119
Ran for 6050 frames Cell Events: 223 mean_circ: 0.945161
Ran for 6550 frames Cell Events: 245 mean_circ: 0.944731
Ran for 7550 frames Cell Events: 304 mean_circ: 0.943288
Ran for 8550 frames Cell Events: 344 mean_circ: 0.94264
Ran for 10050 frames Cell Events: 393 mean_circ: 0.943206
Ran for 10550 frames Cell Events: 413 mean_circ: 0.9433
Ran for 12050 frames Cell Events: 481 mean_circ: 0.943424
Ran for 12550 frames Cell Events: 502 mean_circ: 0.943754
Ran for 13550 frames Cell Events: 553 mean_circ: 0.943404
Ran for 14050 frames Cell Events: 573 mean_circ: 0.94319
Ran for 15050 frames Cell Events: 629 mean_circ: 0.942974
Ran for 15550 frames Cell Events: 661 mean_circ: 0.942915
Ran for 16050 frames Cell Events: 693 mean_circ: 0.943224
Ran for 17050 frames Cell Events: 740 mean_circ: 0.943041
Ran for 19050 frames Cell Events: 836 mean_circ: 0.942652
Ran for 21050 frames Cell Events: 932 mean_circ: 0.942642
Ran for 21550 frames Cell Events: 951 mean_circ: 0.942544
Ran for 22050 frames Cell Events: 985 mean_circ: 0.942077
Ran for 22550 frames Cell Events: 1000 mean_circ: 0.942152
Ran for 24050 frames Cell Events: 1073 mean_circ: 0.9423
Ran for 25050 frames Cell Events: 1143 mean_circ: 0.942213
Ran for 26550 frames Cell Events: 1212 mean_circ: 0.942436
Ran for 27050 frames Cell Events: 1224 mean_circ: 0.942438
Ran for 27550 frames Cell Events: 1244 mean_circ: 0.942561
Ran for 28050 frames Cell Events: 1266 mean_circ: 0.94265
Ran for 29550 frames Cell Events: 1341 mean_circ: 0.942972
Ran for 30050 frames Cell Events: 1355 mean_circ: 0.943018
Ran for 31050 frames Cell Events: 1393 mean_circ: 0.943135
Ran for 33050 frames Cell Events: 1469 mean_circ: 0.943432
Ran for 34550 frames Cell Events: 1526 mean_circ: 0.943698
Ran for 35550 frames Cell Events: 1568 mean_circ: 0.9436
Ran for 36050 frames Cell Events: 1591 mean_circ: 0.943606
Ran for 37050 frames Cell Events: 1632 mean_circ: 0.943356
Ran for 37550 frames Cell Events: 1646 mean_circ: 0.943431
Ran for 38050 frames Cell Events: 1667 mean_circ: 0.943386
Ran for 39050 frames Cell Events: 1707 mean_circ: 0.943181
Ran for 40050 frames Cell Events: 1754 mean_circ: 0.942867
Ran for 41050 frames Cell Events: 1807 mean_circ: 0.942837
Ran for 41550 frames Cell Events: 1830 mean_circ: 0.942897
Ran for 42050 frames Cell Events: 1848 mean_circ: 0.943035
Ran for 43050 frames Cell Events: 1887 mean_circ: 0.942906
Ran for 43550 frames Cell Events: 1909 mean_circ: 0.942953
Ran for 44050 frames Cell Events: 1938 mean_circ: 0.942914
Ran for 45550 frames Cell Events: 2024 mean_circ: 0.942763
Ran for 46050 frames Cell Events: 2045 mean_circ: 0.942773
Ran for 49050 frames Cell Events: 2174 mean_circ: 0.94264
Ran for 50050 frames Cell Events: 2215 mean_circ: 0.942657
Ran for 53050 frames Cell Events: 2366 mean_circ: 0.942638
Ran for 53550 frames Cell Events: 2388 mean_circ: 0.942541
Ran for 55050 frames Cell Events: 2454 mean_circ: 0.942555
Ran for 56550 frames Cell Events: 2522 mean_circ: 0.942528
Ran for 57050 frames Cell Events: 2539 mean_circ: 0.942442
Ran for 58050 frames Cell Events: 2588 mean_circ: 0.942307
Ran for 59050 frames Cell Events: 2643 mean_circ: 0.94251
Ran for 59550 frames Cell Events: 2659 mean_circ: 0.942366
Ran for 60550 frames Cell Events: 2711 mean_circ: 0.942247
Ran for 61550 frames Cell Events: 2765 mean_circ: 0.942281
Ran for 63550 frames Cell Events: 2870 mean_circ: 0.942199
Ran for 64550 frames Cell Events: 2930 mean_circ: 0.942206
Ran for 66550 frames Cell Events: 3010 mean_circ: 0.942077
Ran for 68050 frames Cell Events: 3084 mean_circ: 0.941976
Ran for 68550 frames Cell Events: 3110 mean_circ: 0.94209
Ran for 71050 frames Cell Events: 3242 mean_circ: 0.942038
Ran for 73050 frames Cell Events: 3401 mean_circ: 0.941959
Ran for 74050 frames Cell Events: 3440 mean_circ: 0.941923
Ran for 79050 frames Cell Events: 3676 mean_circ: 0.941945
Ran for 80050 frames Cell Events: 3730 mean_circ: 0.941911
Ran for 80550 frames Cell Events: 3755 mean_circ: 0.941868
Ran for 81050 frames Cell Events: 3773 mean_circ: 0.941844
Ran for 81550 frames Cell Events: 3790 mean_circ: 0.941877
Ran for 82050 frames Cell Events: 3809 mean_circ: 0.941843
Ran for 83550 frames Cell Events: 3868 mean_circ: 0.941765
Ran for 84050 frames Cell Events: 3891 mean_circ: 0.941839
Ran for 87550 frames Cell Events: 4071 mean_circ: 0.94186
Ran for 89050 frames Cell Events: 4157 mean_circ: 0.941593
Ran for 90050 frames Cell Events: 4201 mean_circ: 0.941586
Ran for 90550 frames Cell Events: 4229 mean_circ: 0.941517
Ran for 91050 frames Cell Events: 4251 mean_circ: 0.94157
Ran for 92050 frames Cell Events: 4303 mean_circ: 0.941562
Ran for 92550 frames Cell Events: 4332 mean_circ: 0.94159
Ran for 93550 frames Cell Events: 4398 mean_circ: 0.941682
Ran for 94050 frames Cell Events: 4419 mean_circ: 0.941703
Ran for 94550 frames Cell Events: 4446 mean_circ: 0.941681
Ran for 97550 frames Cell Events: 4592 mean_circ: 0.941658
Ran for 98550 frames Cell Events: 4632 mean_circ: 0.941584
Ran for 99050 frames Cell Events: 4654 mean_circ: 0.941566
Ran for 99550 frames Cell Events: 4668 mean_circ: 0.941525
Ran for 100550 frames Cell Events: 4720 mean_circ: 0.941512
Ran for 101550 frames Cell Events: 4759 mean_circ: 0.941499
Ran for 102050 frames Cell Events: 4786 mean_circ: 0.941499
Ran for 102550 frames Cell Events: 4806 mean_circ: 0.941494
Ran for 103050 frames Cell Events: 4829 mean_circ: 0.941487
Ran for 104550 frames Cell Events: 4897 mean_circ: 0.941491
Ran for 105050 frames Cell Events: 4913 mean_circ: 0.941489
Ran for 105550 frames Cell Events: 4925 mean_circ: 0.941525
Ran for 106050 frames Cell Events: 4948 mean_circ: 0.941529
Ran for 108050 frames Cell Events: 5054 mean_circ: 0.941465
Ran for 108550 frames Cell Events: 5069 mean_circ: 0.941464
Ran for 109050 frames Cell Events: 5085 mean_circ: 0.941479
Ran for 109550 frames Cell Events: 5100 mean_circ: 0.941499
Ran for 110550 frames Cell Events: 5154 mean_circ: 0.941503
Ran for 111050 frames Cell Events: 5187 mean_circ: 0.941538
Ran for 111550 frames Cell Events: 5209 mean_circ: 0.941546
Ran for 112050 frames Cell Events: 5230 mean_circ: 0.94156
Ran for 112550 frames Cell Events: 5244 mean_circ: 0.941528
Ran for 113050 frames Cell Events: 5265 mean_circ: 0.941482
Ran for 114050 frames Cell Events: 5311 mean_circ: 0.9415
Ran for 114550 frames Cell Events: 5335 mean_circ: 0.94145
Ran for 115050 frames Cell Events: 5359 mean_circ: 0.941487
Ran for 117050 frames Cell Events: 5466 mean_circ: 0.941417
Ran for 117550 frames Cell Events: 5485 mean_circ: 0.941402
Ran for 121050 frames Cell Events: 5669 mean_circ: 0.941427
Ran for 121550 frames Cell Events: 5692 mean_circ: 0.941391
Ran for 123550 frames Cell Events: 5792 mean_circ: 0.941464
Ran for 125050 frames Cell Events: 5877 mean_circ: 0.941366
Ran for 125550 frames Cell Events: 5904 mean_circ: 0.941403
Ran for 127050 frames Cell Events: 5983 mean_circ: 0.941455
Ran for 128050 frames Cell Events: 6021 mean_circ: 0.94139
Ran for 128550 frames Cell Events: 6040 mean_circ: 0.9414
Ran for 129550 frames Cell Events: 6091 mean_circ: 0.941384
Ran for 130050 frames Cell Events: 6114 mean_circ: 0.941405
Ran for 131050 frames Cell Events: 6157 mean_circ: 0.941376
Ran for 132050 frames Cell Events: 6235 mean_circ: 0.941367
Ran for 133050 frames Cell Events: 6291 mean_circ: 0.941352
Ran for 133550 frames Cell Events: 6309 mean_circ: 0.941396
Ran for 134050 frames Cell Events: 6335 mean_circ: 0.941438
Ran for 134550 frames Cell Events: 6354 mean_circ: 0.941453
Ran for 135550 frames Cell Events: 6403 mean_circ: 0.941336
Ran for 137050 frames Cell Events: 6487 mean_circ: 0.941277
Ran for 138550 frames Cell Events: 6554 mean_circ: 0.941292
Ran for 139050 frames Cell Events: 6580 mean_circ: 0.941329
Ran for 139550 frames Cell Events: 6609 mean_circ: 0.941329
Ran for 140050 frames Cell Events: 6637 mean_circ: 0.941371
Ran for 140550 frames Cell Events: 6662 mean_circ: 0.941346
Ran for 141050 frames Cell Events: 6678 mean_circ: 0.941312
Ran for 141550 frames Cell Events: 6695 mean_circ: 0.941243
Ran for 142550 frames Cell Events: 6725 mean_circ: 0.941259
Ran for 143550 frames Cell Events: 6779 mean_circ: 0.941248
Ran for 145050 frames Cell Events: 6856 mean_circ: 0.941303
Ran for 145550 frames Cell Events: 6875 mean_circ: 0.941323
Ran for 146050 frames Cell Events: 6896 mean_circ: 0.941322
Ran for 147550 frames Cell Events: 6962 mean_circ: 0.941358
Ran for 148050 frames Cell Events: 6981 mean_circ: 0.941361
Ran for 150550 frames Cell Events: 7105 mean_circ: 0.941289
Ran for 152550 frames Cell Events: 7188 mean_circ: 0.941302
Ran for 153550 frames Cell Events: 7236 mean_circ: 0.941333
Ran for 154550 frames Cell Events: 7292 mean_circ: 0.941298
Ran for 155550 frames Cell Events: 7334 mean_circ: 0.941365
Ran for 156050 frames Cell Events: 7354 mean_circ: 0.94136
Ran for 156550 frames Cell Events: 7376 mean_circ: 0.941371
Ran for 158050 frames Cell Events: 7434 mean_circ: 0.941431
Ran for 159550 frames Cell Events: 7516 mean_circ: 0.941384
Ran for 160550 frames Cell Events: 7576 mean_circ: 0.941313
Ran for 161550 frames Cell Events: 7627 mean_circ: 0.941354
Ran for 162050 frames Cell Events: 7653 mean_circ: 0.9413
Ran for 163050 frames Cell Events: 7707 mean_circ: 0.941312
Ran for 165550 frames Cell Events: 7846 mean_circ: 0.94133
Ran for 166550 frames Cell Events: 7894 mean_circ: 0.941251
Ran for 167050 frames Cell Events: 7917 mean_circ: 0.94127
Ran for 167550 frames Cell Events: 7944 mean_circ: 0.941265
Ran for 168050 frames Cell Events: 7955 mean_circ: 0.941261
Ran for 168550 frames Cell Events: 7978 mean_circ: 0.941255
Ran for 169550 frames Cell Events: 8020 mean_circ: 0.941243



End this measurment..



Stopping time: 2.4452e+009
****************************************
Measurement finished

Mean Circularitiy: 0.941272
Mean Area: 589.371
Mean FL-1 intensity: 2941.01
Mean FL-2 intensity: 3829.03
Mean FL-3 intensity: 0

frames processed: 169119
Total Time: 85192.5ms
Number of Runs (new bg img) 0
Frames collected: 170218
Skipped frames: 31675
Rescued Frames: 2405852
Samples DAQ: 42554483
Samples written: 85108754
Samples per Channel: 42554377
FPS: 1998.04

Samples per second: 499508
Expected samples/s: 500000

Events: 8041
Events per second: 94.3862
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :255805
# 1 frames :69739
# 2 frames :4293
# 3 frames :1276
# 4 frames :779
# 5 frames :358
# 6 frames :1541
# 7 frames :1585
# 8 frames :972
# 9 frames :666
# 10 frames :689
# 11 frames :763
# 12 frames :388
# 13 frames :239
# 14 frames :179
# 15 frames :173
# 16 frames :165
# 17 frames :147
# 18 frames :102
# 19 frames :68
# 20 frames :69
# 21 frames :52
# 22 frames :53
# 23 frames :46
# 24 frames :33
# 25 frames :30
# 26 frames :33
# 27 frames :13
# 28 frames :14
# 29 frames :9
# 30 frames :13
# 31 frames :7
# 32 frames :6
# 33 frames :11
# 34 frames :6
# 35 frames :9
# 36 frames :4
# 37 frames :7
# 38 frames :7
# 39 frames :5
# 40 frames :3
# 41 frames :3
# 42 frames :2
# 44 frames :1
# 45 frames :2
# 46 frames :3
# 47 frames :1
# 48 frames :2
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :2
# 56 frames :1
# 58 frames :2
# 59 frames :2
# 61 frames :1
# 67 frames :1
# 70 frames :1
# 77 frames :1
# 79 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7825
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0