Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.52.26 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "91ba821d08a8163b948e65e6d18172ea",
"name": "M1_20170612_1853_dsRNA_U7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:52:49"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9359 (max 9360) in '9359'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9014
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.45704e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 10 mean_circ: 0.949266
Ran for 1550 frames Cell Events: 35 mean_circ: 0.951278
Ran for 3550 frames Cell Events: 90 mean_circ: 0.955483
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Ran for 5050 frames Cell Events: 141 mean_circ: 0.953252
Ran for 6550 frames Cell Events: 197 mean_circ: 0.950564
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Ran for 10550 frames Cell Events: 305 mean_circ: 0.950166
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Ran for 11550 frames Cell Events: 348 mean_circ: 0.94947
Ran for 13550 frames Cell Events: 427 mean_circ: 0.950152
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Ran for 97050 frames Cell Events: 3212 mean_circ: 0.951479
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Ran for 100050 frames Cell Events: 3309 mean_circ: 0.951747
Ran for 102550 frames Cell Events: 3402 mean_circ: 0.95174
Ran for 103050 frames Cell Events: 3423 mean_circ: 0.951725
Ran for 103550 frames Cell Events: 3436 mean_circ: 0.951683
Ran for 104050 frames Cell Events: 3456 mean_circ: 0.951719
Ran for 104550 frames Cell Events: 3478 mean_circ: 0.951714
Ran for 107050 frames Cell Events: 3568 mean_circ: 0.951671
Ran for 108050 frames Cell Events: 3597 mean_circ: 0.951669
Ran for 108550 frames Cell Events: 3604 mean_circ: 0.951683
Ran for 109550 frames Cell Events: 3648 mean_circ: 0.951565
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Ran for 126550 frames Cell Events: 4266 mean_circ: 0.951804
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Ran for 143050 frames Cell Events: 4883 mean_circ: 0.951718
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Ran for 147550 frames Cell Events: 5061 mean_circ: 0.951708
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Ran for 162550 frames Cell Events: 5612 mean_circ: 0.951627
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Ran for 167050 frames Cell Events: 5803 mean_circ: 0.951748
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Ran for 217550 frames Cell Events: 7627 mean_circ: 0.951713
Ran for 218550 frames Cell Events: 7662 mean_circ: 0.951721
Ran for 220050 frames Cell Events: 7712 mean_circ: 0.951717
Ran for 221050 frames Cell Events: 7742 mean_circ: 0.951703
Ran for 221550 frames Cell Events: 7765 mean_circ: 0.951701
Ran for 222050 frames Cell Events: 7775 mean_circ: 0.951686
Ran for 223050 frames Cell Events: 7803 mean_circ: 0.951729
Ran for 223550 frames Cell Events: 7819 mean_circ: 0.95173
Ran for 224050 frames Cell Events: 7831 mean_circ: 0.951698
Ran for 225050 frames Cell Events: 7872 mean_circ: 0.951699
Ran for 225550 frames Cell Events: 7886 mean_circ: 0.951687
Ran for 226550 frames Cell Events: 7929 mean_circ: 0.951659
Ran for 227050 frames Cell Events: 7949 mean_circ: 0.951663
Ran for 228050 frames Cell Events: 7995 mean_circ: 0.951672
Ran for 228550 frames Cell Events: 8011 mean_circ: 0.951668
Ran for 229050 frames Cell Events: 8030 mean_circ: 0.951683
Ran for 229550 frames Cell Events: 8051 mean_circ: 0.951669
Ran for 230550 frames Cell Events: 8089 mean_circ: 0.95167
Ran for 231050 frames Cell Events: 8105 mean_circ: 0.951652
Ran for 231550 frames Cell Events: 8124 mean_circ: 0.95164
Ran for 232550 frames Cell Events: 8159 mean_circ: 0.95163
Ran for 233050 frames Cell Events: 8173 mean_circ: 0.951619
Ran for 233550 frames Cell Events: 8187 mean_circ: 0.95162
Ran for 234050 frames Cell Events: 8216 mean_circ: 0.951562
Ran for 234550 frames Cell Events: 8234 mean_circ: 0.951556
Ran for 235050 frames Cell Events: 8243 mean_circ: 0.951556
Ran for 235550 frames Cell Events: 8268 mean_circ: 0.951553
Ran for 236050 frames Cell Events: 8287 mean_circ: 0.951594
Ran for 237050 frames Cell Events: 8331 mean_circ: 0.951565
Ran for 238050 frames Cell Events: 8352 mean_circ: 0.951569
Ran for 238550 frames Cell Events: 8364 mean_circ: 0.951577
Ran for 239050 frames Cell Events: 8382 mean_circ: 0.951596
Ran for 240550 frames Cell Events: 8439 mean_circ: 0.951593
Ran for 241050 frames Cell Events: 8454 mean_circ: 0.951612
Ran for 242050 frames Cell Events: 8494 mean_circ: 0.951608
Ran for 243550 frames Cell Events: 8546 mean_circ: 0.951582
Ran for 245050 frames Cell Events: 8596 mean_circ: 0.951583
Ran for 245550 frames Cell Events: 8613 mean_circ: 0.951611
Ran for 246550 frames Cell Events: 8649 mean_circ: 0.951627
Ran for 247550 frames Cell Events: 8690 mean_circ: 0.951598
Ran for 249050 frames Cell Events: 8758 mean_circ: 0.951574
Ran for 249550 frames Cell Events: 8775 mean_circ: 0.951605
Ran for 250050 frames Cell Events: 8792 mean_circ: 0.951601
Ran for 250550 frames Cell Events: 8818 mean_circ: 0.951615
Ran for 252050 frames Cell Events: 8865 mean_circ: 0.95165
Ran for 253050 frames Cell Events: 8897 mean_circ: 0.951603
Ran for 253550 frames Cell Events: 8922 mean_circ: 0.951599
Ran for 254050 frames Cell Events: 8941 mean_circ: 0.951593
Ran for 255550 frames Cell Events: 9012 mean_circ: 0.951583
Ran for 256050 frames Cell Events: 9033 mean_circ: 0.951599
Ran for 256550 frames Cell Events: 9053 mean_circ: 0.951604
Ran for 257050 frames Cell Events: 9071 mean_circ: 0.951622
Ran for 257550 frames Cell Events: 9093 mean_circ: 0.951611
Ran for 258050 frames Cell Events: 9112 mean_circ: 0.951627
Ran for 258550 frames Cell Events: 9132 mean_circ: 0.951615
Ran for 259050 frames Cell Events: 9151 mean_circ: 0.951615
Ran for 259550 frames Cell Events: 9167 mean_circ: 0.95162
Ran for 260050 frames Cell Events: 9185 mean_circ: 0.951608
Ran for 261050 frames Cell Events: 9230 mean_circ: 0.951628
Ran for 261550 frames Cell Events: 9254 mean_circ: 0.951648
Ran for 262550 frames Cell Events: 9304 mean_circ: 0.95166
Ran for 263050 frames Cell Events: 9322 mean_circ: 0.951671



End this measurment..



Stopping time: 2.45717e+009
****************************************
Measurement finished

Mean Circularitiy: 0.951687
Mean Area: 625.559
Mean FL-1 intensity: 3199.93
Mean FL-2 intensity: 3790.97
Mean FL-3 intensity: 0

frames processed: 263141
Total Time: 132025ms
Number of Runs (new bg img) 0
Frames collected: 263898
Skipped frames: 25075
Rescued Frames: 2695060
Samples DAQ: 65974472
Samples written: 131948786
Samples per Channel: 65974393
FPS: 1998.84

Samples per second: 499710
Expected samples/s: 500000

Events: 9360
Events per second: 70.8954
jumped back (PMT): 5384
#Frames read, frequency
# 0 frames :381578
# 1 frames :126074
# 2 frames :5598
# 3 frames :2123
# 4 frames :1229
# 5 frames :664
# 6 frames :1227
# 7 frames :2135
# 8 frames :1259
# 9 frames :856
# 10 frames :1110
# 11 frames :943
# 12 frames :611
# 13 frames :415
# 14 frames :281
# 15 frames :264
# 16 frames :241
# 17 frames :224
# 18 frames :174
# 19 frames :152
# 20 frames :116
# 21 frames :80
# 22 frames :65
# 23 frames :51
# 24 frames :44
# 25 frames :37
# 26 frames :35
# 27 frames :38
# 28 frames :22
# 29 frames :23
# 30 frames :22
# 31 frames :16
# 32 frames :9
# 33 frames :8
# 34 frames :8
# 35 frames :7
# 36 frames :3
# 37 frames :3
# 39 frames :6
# 40 frames :3
# 41 frames :3
# 42 frames :1
# 43 frames :2
# 44 frames :3
# 45 frames :3
# 46 frames :2
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 52 frames :1
# 58 frames :1
# 60 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9014
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0