Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.52.56 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "502ec480f2b2cf0f1be6a27a17a04b6e",
"name": "M1_20170612_1857_dsRNA_U7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:53:16"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8111 (max 8112) in '8111'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8823
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.45725e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 40 mean_circ: 0.943802
Ran for 1550 frames Cell Events: 59 mean_circ: 0.941774
Ran for 2050 frames Cell Events: 77 mean_circ: 0.944748
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Ran for 3550 frames Cell Events: 136 mean_circ: 0.943464
Ran for 4050 frames Cell Events: 157 mean_circ: 0.943148
Ran for 5050 frames Cell Events: 198 mean_circ: 0.94367
Ran for 5550 frames Cell Events: 212 mean_circ: 0.944069
Ran for 6050 frames Cell Events: 238 mean_circ: 0.943736
Ran for 6550 frames Cell Events: 257 mean_circ: 0.943741
Ran for 7050 frames Cell Events: 278 mean_circ: 0.943768
Ran for 8550 frames Cell Events: 345 mean_circ: 0.943807
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Ran for 10050 frames Cell Events: 394 mean_circ: 0.942862
Ran for 11050 frames Cell Events: 433 mean_circ: 0.942788
Ran for 12050 frames Cell Events: 475 mean_circ: 0.943441
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Ran for 13050 frames Cell Events: 516 mean_circ: 0.943828
Ran for 13550 frames Cell Events: 525 mean_circ: 0.943665
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Ran for 15050 frames Cell Events: 577 mean_circ: 0.943144
Ran for 16550 frames Cell Events: 629 mean_circ: 0.942667
Ran for 17050 frames Cell Events: 650 mean_circ: 0.942646
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Ran for 23550 frames Cell Events: 892 mean_circ: 0.942672
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Ran for 50050 frames Cell Events: 1905 mean_circ: 0.940961
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Ran for 62550 frames Cell Events: 2359 mean_circ: 0.940687
Ran for 63550 frames Cell Events: 2396 mean_circ: 0.940697
Ran for 64550 frames Cell Events: 2434 mean_circ: 0.940584
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Ran for 67050 frames Cell Events: 2509 mean_circ: 0.940506
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Ran for 69050 frames Cell Events: 2571 mean_circ: 0.940272
Ran for 69550 frames Cell Events: 2588 mean_circ: 0.940261
Ran for 70050 frames Cell Events: 2602 mean_circ: 0.940298
Ran for 70550 frames Cell Events: 2614 mean_circ: 0.940316
Ran for 72550 frames Cell Events: 2689 mean_circ: 0.940365
Ran for 73050 frames Cell Events: 2714 mean_circ: 0.940384
Ran for 73550 frames Cell Events: 2737 mean_circ: 0.940339
Ran for 74050 frames Cell Events: 2771 mean_circ: 0.940128
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Ran for 81050 frames Cell Events: 3039 mean_circ: 0.939913
Ran for 81550 frames Cell Events: 3056 mean_circ: 0.939978
Ran for 83050 frames Cell Events: 3110 mean_circ: 0.940002
Ran for 83550 frames Cell Events: 3125 mean_circ: 0.939942
Ran for 84050 frames Cell Events: 3140 mean_circ: 0.939968
Ran for 84550 frames Cell Events: 3156 mean_circ: 0.939982
Ran for 85050 frames Cell Events: 3176 mean_circ: 0.939932
Ran for 86050 frames Cell Events: 3222 mean_circ: 0.940017
Ran for 86550 frames Cell Events: 3237 mean_circ: 0.939986
Ran for 87550 frames Cell Events: 3283 mean_circ: 0.939816
Ran for 88050 frames Cell Events: 3309 mean_circ: 0.939741
Ran for 89050 frames Cell Events: 3351 mean_circ: 0.939733
Ran for 90550 frames Cell Events: 3415 mean_circ: 0.939696
Ran for 91050 frames Cell Events: 3428 mean_circ: 0.939689
Ran for 91550 frames Cell Events: 3453 mean_circ: 0.939681
Ran for 92050 frames Cell Events: 3472 mean_circ: 0.939678
Ran for 93050 frames Cell Events: 3504 mean_circ: 0.939682
Ran for 93550 frames Cell Events: 3528 mean_circ: 0.939722
Ran for 94550 frames Cell Events: 3564 mean_circ: 0.939815
Ran for 95050 frames Cell Events: 3587 mean_circ: 0.939828
Ran for 96050 frames Cell Events: 3625 mean_circ: 0.939855
Ran for 97050 frames Cell Events: 3667 mean_circ: 0.93977
Ran for 97550 frames Cell Events: 3678 mean_circ: 0.939811
Ran for 98050 frames Cell Events: 3696 mean_circ: 0.939842
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Ran for 101050 frames Cell Events: 3804 mean_circ: 0.939896
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Ran for 103550 frames Cell Events: 3895 mean_circ: 0.93982
Ran for 104050 frames Cell Events: 3910 mean_circ: 0.939842
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Ran for 105550 frames Cell Events: 3969 mean_circ: 0.939856
Ran for 106550 frames Cell Events: 4023 mean_circ: 0.93978
Ran for 107550 frames Cell Events: 4067 mean_circ: 0.939828
Ran for 108050 frames Cell Events: 4088 mean_circ: 0.939867
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Ran for 111050 frames Cell Events: 4188 mean_circ: 0.939972
Ran for 112550 frames Cell Events: 4241 mean_circ: 0.939882
Ran for 113550 frames Cell Events: 4279 mean_circ: 0.939841
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Ran for 116050 frames Cell Events: 4374 mean_circ: 0.939682
Ran for 118050 frames Cell Events: 4454 mean_circ: 0.939762
Ran for 118550 frames Cell Events: 4467 mean_circ: 0.939752
Ran for 119550 frames Cell Events: 4506 mean_circ: 0.939674
Ran for 120050 frames Cell Events: 4529 mean_circ: 0.939708
Ran for 121050 frames Cell Events: 4575 mean_circ: 0.939785
Ran for 121550 frames Cell Events: 4595 mean_circ: 0.939765
Ran for 123050 frames Cell Events: 4672 mean_circ: 0.939698
Ran for 124550 frames Cell Events: 4730 mean_circ: 0.939745
Ran for 125050 frames Cell Events: 4749 mean_circ: 0.939777
Ran for 125550 frames Cell Events: 4776 mean_circ: 0.93976
Ran for 126550 frames Cell Events: 4810 mean_circ: 0.939744
Ran for 127050 frames Cell Events: 4830 mean_circ: 0.939709
Ran for 127550 frames Cell Events: 4850 mean_circ: 0.939672
Ran for 128050 frames Cell Events: 4869 mean_circ: 0.939677
Ran for 129050 frames Cell Events: 4927 mean_circ: 0.939663
Ran for 129550 frames Cell Events: 4952 mean_circ: 0.939653
Ran for 130050 frames Cell Events: 4974 mean_circ: 0.939749
Ran for 130550 frames Cell Events: 4992 mean_circ: 0.939734
Ran for 131050 frames Cell Events: 5009 mean_circ: 0.939721
Ran for 132050 frames Cell Events: 5046 mean_circ: 0.939756
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Ran for 136050 frames Cell Events: 5216 mean_circ: 0.939744
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Ran for 143050 frames Cell Events: 5477 mean_circ: 0.939865
Ran for 143550 frames Cell Events: 5512 mean_circ: 0.939889
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Ran for 147550 frames Cell Events: 5640 mean_circ: 0.939815
Ran for 148550 frames Cell Events: 5670 mean_circ: 0.939801
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Ran for 150050 frames Cell Events: 5736 mean_circ: 0.939699
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Ran for 158050 frames Cell Events: 6050 mean_circ: 0.939706
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Ran for 174050 frames Cell Events: 6640 mean_circ: 0.939553
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Ran for 176050 frames Cell Events: 6711 mean_circ: 0.939533
Ran for 176550 frames Cell Events: 6733 mean_circ: 0.939425
Ran for 177050 frames Cell Events: 6747 mean_circ: 0.939429
Ran for 177550 frames Cell Events: 6765 mean_circ: 0.939402
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Ran for 180550 frames Cell Events: 6881 mean_circ: 0.939285
Ran for 181050 frames Cell Events: 6895 mean_circ: 0.939308
Ran for 182550 frames Cell Events: 6969 mean_circ: 0.939266
Ran for 183050 frames Cell Events: 6992 mean_circ: 0.939304
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Ran for 197050 frames Cell Events: 7577 mean_circ: 0.939242
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Ran for 201550 frames Cell Events: 7763 mean_circ: 0.939208
Ran for 202550 frames Cell Events: 7801 mean_circ: 0.939244
Ran for 204050 frames Cell Events: 7877 mean_circ: 0.939224
Ran for 205550 frames Cell Events: 7954 mean_circ: 0.939191
Ran for 206050 frames Cell Events: 7970 mean_circ: 0.939242
Ran for 206550 frames Cell Events: 7987 mean_circ: 0.939239
Ran for 207050 frames Cell Events: 7995 mean_circ: 0.939252
Ran for 207550 frames Cell Events: 8014 mean_circ: 0.939235
Ran for 208050 frames Cell Events: 8027 mean_circ: 0.939226
Ran for 209050 frames Cell Events: 8056 mean_circ: 0.939256
Ran for 210050 frames Cell Events: 8099 mean_circ: 0.939235



End this measurment..



Stopping time: 2.45736e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93923
Mean Area: 632.208
Mean FL-1 intensity: 3091.63
Mean FL-2 intensity: 4296.9
Mean FL-3 intensity: 0

frames processed: 210198
Total Time: 105222ms
Number of Runs (new bg img) 0
Frames collected: 210332
Skipped frames: 25209
Rescued Frames: 2787371
Samples DAQ: 52583350
Samples written: 105165762
Samples per Channel: 52582881
FPS: 1998.94

Samples per second: 499734
Expected samples/s: 500000

Events: 8112
Events per second: 77.0943
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :302740
# 1 frames :101082
# 2 frames :4694
# 3 frames :1750
# 4 frames :1025
# 5 frames :466
# 6 frames :1327
# 7 frames :1942
# 8 frames :1068
# 9 frames :667
# 10 frames :1035
# 11 frames :804
# 12 frames :504
# 13 frames :289
# 14 frames :207
# 15 frames :158
# 16 frames :179
# 17 frames :147
# 18 frames :99
# 19 frames :83
# 20 frames :61
# 21 frames :50
# 22 frames :42
# 23 frames :37
# 24 frames :41
# 25 frames :22
# 26 frames :25
# 27 frames :12
# 28 frames :7
# 29 frames :10
# 30 frames :14
# 31 frames :4
# 32 frames :4
# 33 frames :5
# 34 frames :1
# 35 frames :4
# 36 frames :2
# 37 frames :2
# 38 frames :1
# 39 frames :2
# 40 frames :3
# 41 frames :2
# 42 frames :2
# 43 frames :3
# 44 frames :1
# 45 frames :1
# 46 frames :2
# 48 frames :1
# 54 frames :1
# 80 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8823
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0