Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.53.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fd55c2d7788439176a948e3898157fea",
"name": "M1_20170612_1905_dsRNA_T2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:53:45"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8562 (max 8563) in '8562'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8382
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.45772e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 12 mean_circ: 0.947434
Ran for 1550 frames Cell Events: 40 mean_circ: 0.951903
Ran for 2050 frames Cell Events: 55 mean_circ: 0.948687
Ran for 3050 frames Cell Events: 94 mean_circ: 0.949513
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Ran for 4050 frames Cell Events: 127 mean_circ: 0.949723
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Ran for 5550 frames Cell Events: 181 mean_circ: 0.951876
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Ran for 8050 frames Cell Events: 273 mean_circ: 0.952143
Ran for 8550 frames Cell Events: 283 mean_circ: 0.952102
Ran for 9550 frames Cell Events: 310 mean_circ: 0.951483
Ran for 10050 frames Cell Events: 322 mean_circ: 0.94942
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Ran for 72550 frames Cell Events: 1973 mean_circ: 0.95186
Ran for 73050 frames Cell Events: 1986 mean_circ: 0.951837
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Ran for 122050 frames Cell Events: 3302 mean_circ: 0.951461
Ran for 122550 frames Cell Events: 3316 mean_circ: 0.951456
Ran for 123050 frames Cell Events: 3327 mean_circ: 0.951464
Ran for 124050 frames Cell Events: 3353 mean_circ: 0.95139
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Ran for 126050 frames Cell Events: 3419 mean_circ: 0.95129
Ran for 127550 frames Cell Events: 3453 mean_circ: 0.95128
Ran for 128050 frames Cell Events: 3465 mean_circ: 0.951278
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Ran for 188050 frames Cell Events: 5317 mean_circ: 0.95105
Ran for 188550 frames Cell Events: 5331 mean_circ: 0.951082
Ran for 189050 frames Cell Events: 5354 mean_circ: 0.951113
Ran for 190550 frames Cell Events: 5401 mean_circ: 0.951137
Ran for 191050 frames Cell Events: 5414 mean_circ: 0.951161
Ran for 191550 frames Cell Events: 5423 mean_circ: 0.951182
Ran for 192050 frames Cell Events: 5438 mean_circ: 0.951193
Ran for 192550 frames Cell Events: 5456 mean_circ: 0.951206
Ran for 193050 frames Cell Events: 5467 mean_circ: 0.951199
Ran for 193550 frames Cell Events: 5479 mean_circ: 0.951208
Ran for 195050 frames Cell Events: 5534 mean_circ: 0.951298
Ran for 195550 frames Cell Events: 5546 mean_circ: 0.951301
Ran for 196550 frames Cell Events: 5577 mean_circ: 0.951258
Ran for 197050 frames Cell Events: 5607 mean_circ: 0.951227
Ran for 198050 frames Cell Events: 5633 mean_circ: 0.951197
Ran for 198550 frames Cell Events: 5645 mean_circ: 0.951185
Ran for 199550 frames Cell Events: 5680 mean_circ: 0.951151
Ran for 200050 frames Cell Events: 5691 mean_circ: 0.951143
Ran for 200550 frames Cell Events: 5706 mean_circ: 0.951171
Ran for 201050 frames Cell Events: 5721 mean_circ: 0.951122
Ran for 201550 frames Cell Events: 5741 mean_circ: 0.95113
Ran for 202050 frames Cell Events: 5757 mean_circ: 0.951135
Ran for 202550 frames Cell Events: 5780 mean_circ: 0.951143
Ran for 203050 frames Cell Events: 5804 mean_circ: 0.951177
Ran for 203550 frames Cell Events: 5816 mean_circ: 0.951146
Ran for 205050 frames Cell Events: 5875 mean_circ: 0.951087
Ran for 206050 frames Cell Events: 5905 mean_circ: 0.950979
Ran for 206550 frames Cell Events: 5922 mean_circ: 0.950981
Ran for 207050 frames Cell Events: 5942 mean_circ: 0.950964
Ran for 207550 frames Cell Events: 5952 mean_circ: 0.950979
Ran for 208050 frames Cell Events: 5962 mean_circ: 0.951001
Ran for 208550 frames Cell Events: 5975 mean_circ: 0.951012
Ran for 209050 frames Cell Events: 5986 mean_circ: 0.951003
Ran for 209550 frames Cell Events: 6002 mean_circ: 0.951028
Ran for 210050 frames Cell Events: 6016 mean_circ: 0.951
Ran for 210550 frames Cell Events: 6024 mean_circ: 0.951008
Ran for 211050 frames Cell Events: 6043 mean_circ: 0.950987
Ran for 212050 frames Cell Events: 6070 mean_circ: 0.951047
Ran for 213550 frames Cell Events: 6128 mean_circ: 0.951025
Ran for 214050 frames Cell Events: 6140 mean_circ: 0.950977
Ran for 214550 frames Cell Events: 6153 mean_circ: 0.950949
Ran for 215050 frames Cell Events: 6168 mean_circ: 0.950948
Ran for 215550 frames Cell Events: 6184 mean_circ: 0.950962
Ran for 216050 frames Cell Events: 6204 mean_circ: 0.950988
Ran for 216550 frames Cell Events: 6218 mean_circ: 0.951009
Ran for 217050 frames Cell Events: 6231 mean_circ: 0.951022
Ran for 218050 frames Cell Events: 6263 mean_circ: 0.95106
Ran for 219550 frames Cell Events: 6305 mean_circ: 0.951083
Ran for 220050 frames Cell Events: 6318 mean_circ: 0.951084
Ran for 220550 frames Cell Events: 6335 mean_circ: 0.951085
Ran for 221050 frames Cell Events: 6350 mean_circ: 0.951063
Ran for 221550 frames Cell Events: 6359 mean_circ: 0.951062
Ran for 222050 frames Cell Events: 6373 mean_circ: 0.951053
Ran for 222550 frames Cell Events: 6385 mean_circ: 0.951066
Ran for 223050 frames Cell Events: 6404 mean_circ: 0.951065
Ran for 223550 frames Cell Events: 6421 mean_circ: 0.951046
Ran for 224550 frames Cell Events: 6462 mean_circ: 0.951051
Ran for 225550 frames Cell Events: 6478 mean_circ: 0.951001
Ran for 226050 frames Cell Events: 6493 mean_circ: 0.950998
Ran for 228050 frames Cell Events: 6537 mean_circ: 0.950969
Ran for 229050 frames Cell Events: 6570 mean_circ: 0.950948
Ran for 229550 frames Cell Events: 6583 mean_circ: 0.950957
Ran for 230050 frames Cell Events: 6601 mean_circ: 0.950955
Ran for 231550 frames Cell Events: 6642 mean_circ: 0.950968
Ran for 233050 frames Cell Events: 6687 mean_circ: 0.950925
Ran for 233550 frames Cell Events: 6703 mean_circ: 0.950923
Ran for 235550 frames Cell Events: 6760 mean_circ: 0.950813
Ran for 236050 frames Cell Events: 6778 mean_circ: 0.950843
Ran for 236550 frames Cell Events: 6797 mean_circ: 0.950836
Ran for 237050 frames Cell Events: 6810 mean_circ: 0.950856
Ran for 237550 frames Cell Events: 6832 mean_circ: 0.950868
Ran for 238050 frames Cell Events: 6846 mean_circ: 0.950885
Ran for 240050 frames Cell Events: 6931 mean_circ: 0.950837
Ran for 240550 frames Cell Events: 6961 mean_circ: 0.950848
Ran for 241050 frames Cell Events: 6979 mean_circ: 0.95086
Ran for 242050 frames Cell Events: 7026 mean_circ: 0.950831
Ran for 242550 frames Cell Events: 7039 mean_circ: 0.950845
Ran for 243050 frames Cell Events: 7047 mean_circ: 0.950853
Ran for 244050 frames Cell Events: 7082 mean_circ: 0.950833
Ran for 244550 frames Cell Events: 7090 mean_circ: 0.950821
Ran for 245050 frames Cell Events: 7098 mean_circ: 0.95082
Ran for 245550 frames Cell Events: 7110 mean_circ: 0.950809
Ran for 246550 frames Cell Events: 7143 mean_circ: 0.950814
Ran for 247050 frames Cell Events: 7159 mean_circ: 0.950799
Ran for 247550 frames Cell Events: 7171 mean_circ: 0.950782
Ran for 248050 frames Cell Events: 7182 mean_circ: 0.950721
Ran for 248550 frames Cell Events: 7197 mean_circ: 0.95072
Ran for 249550 frames Cell Events: 7234 mean_circ: 0.950739
Ran for 250050 frames Cell Events: 7251 mean_circ: 0.950732
Ran for 250550 frames Cell Events: 7271 mean_circ: 0.950761
Ran for 251050 frames Cell Events: 7288 mean_circ: 0.950743
Ran for 252050 frames Cell Events: 7328 mean_circ: 0.950793
Ran for 252550 frames Cell Events: 7346 mean_circ: 0.950782
Ran for 253550 frames Cell Events: 7384 mean_circ: 0.950803
Ran for 254550 frames Cell Events: 7407 mean_circ: 0.950811
Ran for 255050 frames Cell Events: 7424 mean_circ: 0.950814
Ran for 256050 frames Cell Events: 7467 mean_circ: 0.950841
Ran for 256550 frames Cell Events: 7484 mean_circ: 0.950811
Ran for 259550 frames Cell Events: 7587 mean_circ: 0.950833
Ran for 260050 frames Cell Events: 7603 mean_circ: 0.950787
Ran for 260550 frames Cell Events: 7615 mean_circ: 0.950782
Ran for 261050 frames Cell Events: 7627 mean_circ: 0.950773
Ran for 262050 frames Cell Events: 7653 mean_circ: 0.950804
Ran for 263550 frames Cell Events: 7706 mean_circ: 0.950812
Ran for 265550 frames Cell Events: 7773 mean_circ: 0.950841
Ran for 266050 frames Cell Events: 7791 mean_circ: 0.950831
Ran for 267050 frames Cell Events: 7824 mean_circ: 0.950828
Ran for 267550 frames Cell Events: 7842 mean_circ: 0.950833
Ran for 268050 frames Cell Events: 7856 mean_circ: 0.950836
Ran for 269050 frames Cell Events: 7879 mean_circ: 0.950844
Ran for 269550 frames Cell Events: 7894 mean_circ: 0.950864
Ran for 270550 frames Cell Events: 7932 mean_circ: 0.950889
Ran for 271050 frames Cell Events: 7942 mean_circ: 0.950907
Ran for 271550 frames Cell Events: 7952 mean_circ: 0.9509
Ran for 272050 frames Cell Events: 7964 mean_circ: 0.950868
Ran for 273050 frames Cell Events: 7998 mean_circ: 0.950883
Ran for 273550 frames Cell Events: 8013 mean_circ: 0.950872
Ran for 274050 frames Cell Events: 8029 mean_circ: 0.950894
Ran for 274550 frames Cell Events: 8044 mean_circ: 0.950907
Ran for 275050 frames Cell Events: 8058 mean_circ: 0.950917
Ran for 276050 frames Cell Events: 8094 mean_circ: 0.95088
Ran for 276550 frames Cell Events: 8109 mean_circ: 0.95087
Ran for 277550 frames Cell Events: 8146 mean_circ: 0.95087
Ran for 278050 frames Cell Events: 8162 mean_circ: 0.950844
Ran for 278550 frames Cell Events: 8192 mean_circ: 0.950867
Ran for 279050 frames Cell Events: 8206 mean_circ: 0.950846
Ran for 279550 frames Cell Events: 8229 mean_circ: 0.950859
Ran for 280050 frames Cell Events: 8247 mean_circ: 0.950845
Ran for 280550 frames Cell Events: 8267 mean_circ: 0.95086
Ran for 281050 frames Cell Events: 8288 mean_circ: 0.95083
Ran for 281550 frames Cell Events: 8297 mean_circ: 0.950817
Ran for 282050 frames Cell Events: 8310 mean_circ: 0.950791
Ran for 283050 frames Cell Events: 8337 mean_circ: 0.950798
Ran for 283550 frames Cell Events: 8347 mean_circ: 0.950808
Ran for 285050 frames Cell Events: 8419 mean_circ: 0.950819
Ran for 285550 frames Cell Events: 8428 mean_circ: 0.950819
Ran for 286050 frames Cell Events: 8450 mean_circ: 0.950829
Ran for 286550 frames Cell Events: 8462 mean_circ: 0.950783
Ran for 287050 frames Cell Events: 8487 mean_circ: 0.950797
Ran for 287550 frames Cell Events: 8501 mean_circ: 0.950817
Ran for 288050 frames Cell Events: 8512 mean_circ: 0.950804
Ran for 288550 frames Cell Events: 8523 mean_circ: 0.950805
Ran for 289050 frames Cell Events: 8541 mean_circ: 0.950811
Ran for 289550 frames Cell Events: 8554 mean_circ: 0.950819



End this measurment..



Stopping time: 2.45787e+009
****************************************
Measurement finished

Mean Circularitiy: 0.950821
Mean Area: 600.221
Mean FL-1 intensity: 3070.86
Mean FL-2 intensity: 3736.53
Mean FL-3 intensity: 0

frames processed: 289541
Total Time: 144934ms
Number of Runs (new bg img) 0
Frames collected: 289712
Skipped frames: 25380
Rescued Frames: 2909692
Samples DAQ: 72429698
Samples written: 144855758
Samples per Channel: 72427879
FPS: 1998.93

Samples per second: 499731
Expected samples/s: 500000

Events: 8563
Events per second: 59.0822
jumped back (PMT): 5214
#Frames read, frequency
# 0 frames :411956
# 1 frames :146830
# 2 frames :5433
# 3 frames :2467
# 4 frames :1367
# 5 frames :714
# 6 frames :956
# 7 frames :1828
# 8 frames :1320
# 9 frames :912
# 10 frames :818
# 11 frames :1025
# 12 frames :771
# 13 frames :591
# 14 frames :351
# 15 frames :280
# 16 frames :224
# 17 frames :227
# 18 frames :170
# 19 frames :183
# 20 frames :150
# 21 frames :120
# 22 frames :95
# 23 frames :61
# 24 frames :55
# 25 frames :49
# 26 frames :29
# 27 frames :34
# 28 frames :23
# 29 frames :28
# 30 frames :20
# 31 frames :12
# 32 frames :6
# 33 frames :9
# 34 frames :6
# 35 frames :4
# 36 frames :9
# 37 frames :6
# 38 frames :10
# 39 frames :3
# 40 frames :2
# 41 frames :1
# 42 frames :2
# 43 frames :3
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :3
# 48 frames :4
# 49 frames :3
# 50 frames :1
# 53 frames :1
# 55 frames :1
# 63 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8382
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0