Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.57.52 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c8dabcd58fd7d111e2ea83cd756befc3",
"name": "M1_20170612_2004_dsRNA_Z2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:58:13"
}
}
dclab-tdms2rtdc-warnings MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170612_katarzyna_kc167_phospho_screen2\20170612_2004_dsRNA_Z2_faster\M1_20170612_2004_dsRNA_Z2_faster_traces.tdms'
due to multiple values for samples per event: [200 400]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8457 (max 8458) in '8457'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0065
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.46129e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 32 mean_circ: 0.885224
Ran for 1550 frames Cell Events: 49 mean_circ: 0.9001
Ran for 2050 frames Cell Events: 63 mean_circ: 0.893419
Ran for 3550 frames Cell Events: 117 mean_circ: 0.896558
Ran for 4550 frames Cell Events: 153 mean_circ: 0.898194
Ran for 5050 frames Cell Events: 168 mean_circ: 0.895501
Ran for 5550 frames Cell Events: 181 mean_circ: 0.894498
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Ran for 6550 frames Cell Events: 210 mean_circ: 0.896274
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Ran for 11050 frames Cell Events: 353 mean_circ: 0.900151
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Ran for 87050 frames Cell Events: 2878 mean_circ: 0.897779
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Ran for 94050 frames Cell Events: 3138 mean_circ: 0.897438
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Ran for 96050 frames Cell Events: 3174 mean_circ: 0.897262
Ran for 97550 frames Cell Events: 3235 mean_circ: 0.897244
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Ran for 100050 frames Cell Events: 3331 mean_circ: 0.897672
Ran for 101050 frames Cell Events: 3375 mean_circ: 0.897821
Ran for 101550 frames Cell Events: 3386 mean_circ: 0.897936
Ran for 103050 frames Cell Events: 3429 mean_circ: 0.898076
Ran for 103550 frames Cell Events: 3454 mean_circ: 0.897998
Ran for 104050 frames Cell Events: 3470 mean_circ: 0.898066
Ran for 105050 frames Cell Events: 3506 mean_circ: 0.898033
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Ran for 107550 frames Cell Events: 3599 mean_circ: 0.897668
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Ran for 110550 frames Cell Events: 3701 mean_circ: 0.897445
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Ran for 112050 frames Cell Events: 3752 mean_circ: 0.897374
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Ran for 122050 frames Cell Events: 4066 mean_circ: 0.897128
Ran for 123050 frames Cell Events: 4095 mean_circ: 0.897204
Ran for 123550 frames Cell Events: 4108 mean_circ: 0.897236
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Ran for 125050 frames Cell Events: 4149 mean_circ: 0.897243
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Ran for 127550 frames Cell Events: 4221 mean_circ: 0.897398
Ran for 128050 frames Cell Events: 4240 mean_circ: 0.897316
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Ran for 131550 frames Cell Events: 4322 mean_circ: 0.897581
Ran for 132550 frames Cell Events: 4347 mean_circ: 0.897598
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Ran for 142050 frames Cell Events: 4669 mean_circ: 0.897256
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Ran for 143050 frames Cell Events: 4693 mean_circ: 0.897133
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Ran for 144550 frames Cell Events: 4752 mean_circ: 0.897135
Ran for 146050 frames Cell Events: 4797 mean_circ: 0.897116
Ran for 146550 frames Cell Events: 4808 mean_circ: 0.897173
Ran for 147050 frames Cell Events: 4827 mean_circ: 0.897128
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Ran for 192050 frames Cell Events: 6375 mean_circ: 0.896812
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Ran for 197550 frames Cell Events: 6522 mean_circ: 0.896804
Ran for 198050 frames Cell Events: 6541 mean_circ: 0.896734
Ran for 199550 frames Cell Events: 6599 mean_circ: 0.8966
Ran for 200050 frames Cell Events: 6618 mean_circ: 0.896609
Ran for 202050 frames Cell Events: 6681 mean_circ: 0.896501
Ran for 203050 frames Cell Events: 6719 mean_circ: 0.896543
Ran for 203550 frames Cell Events: 6731 mean_circ: 0.896568
Ran for 204050 frames Cell Events: 6746 mean_circ: 0.896557
Ran for 204550 frames Cell Events: 6766 mean_circ: 0.896604
Ran for 205050 frames Cell Events: 6781 mean_circ: 0.896626
Ran for 205550 frames Cell Events: 6797 mean_circ: 0.896612
Ran for 206050 frames Cell Events: 6814 mean_circ: 0.896615
Ran for 206550 frames Cell Events: 6830 mean_circ: 0.896644
Ran for 207050 frames Cell Events: 6849 mean_circ: 0.896664
Ran for 207550 frames Cell Events: 6863 mean_circ: 0.89665
Ran for 208050 frames Cell Events: 6882 mean_circ: 0.896651
Ran for 208550 frames Cell Events: 6906 mean_circ: 0.896673
Ran for 209050 frames Cell Events: 6927 mean_circ: 0.896623
Ran for 209550 frames Cell Events: 6938 mean_circ: 0.896612
Ran for 210050 frames Cell Events: 6951 mean_circ: 0.896621
Ran for 211050 frames Cell Events: 6985 mean_circ: 0.89657
Ran for 211550 frames Cell Events: 7010 mean_circ: 0.896648
Ran for 212050 frames Cell Events: 7034 mean_circ: 0.896636
Ran for 213050 frames Cell Events: 7075 mean_circ: 0.896549
Ran for 214050 frames Cell Events: 7130 mean_circ: 0.896564
Ran for 216050 frames Cell Events: 7221 mean_circ: 0.896537
Ran for 217050 frames Cell Events: 7260 mean_circ: 0.8965
Ran for 217550 frames Cell Events: 7283 mean_circ: 0.896517
Ran for 218050 frames Cell Events: 7300 mean_circ: 0.896517
Ran for 218550 frames Cell Events: 7321 mean_circ: 0.896515
Ran for 219050 frames Cell Events: 7332 mean_circ: 0.896493
Ran for 220550 frames Cell Events: 7399 mean_circ: 0.896479
Ran for 221050 frames Cell Events: 7410 mean_circ: 0.896479
Ran for 221550 frames Cell Events: 7428 mean_circ: 0.896536
Ran for 222050 frames Cell Events: 7442 mean_circ: 0.896494
Ran for 222550 frames Cell Events: 7455 mean_circ: 0.896447
Ran for 223050 frames Cell Events: 7472 mean_circ: 0.896462
Ran for 223550 frames Cell Events: 7491 mean_circ: 0.896445
Ran for 224050 frames Cell Events: 7508 mean_circ: 0.89644
Ran for 224550 frames Cell Events: 7517 mean_circ: 0.896378
Ran for 225050 frames Cell Events: 7529 mean_circ: 0.896404
Ran for 225550 frames Cell Events: 7545 mean_circ: 0.896415
Ran for 226550 frames Cell Events: 7576 mean_circ: 0.89631
Ran for 227050 frames Cell Events: 7585 mean_circ: 0.896319
Ran for 227550 frames Cell Events: 7602 mean_circ: 0.896378
Ran for 229050 frames Cell Events: 7648 mean_circ: 0.896179
Ran for 230050 frames Cell Events: 7680 mean_circ: 0.896187
Ran for 230550 frames Cell Events: 7692 mean_circ: 0.89622
Ran for 231050 frames Cell Events: 7707 mean_circ: 0.896182
Ran for 232050 frames Cell Events: 7743 mean_circ: 0.896132
Ran for 232550 frames Cell Events: 7759 mean_circ: 0.896163
Ran for 233050 frames Cell Events: 7772 mean_circ: 0.896129
Ran for 233550 frames Cell Events: 7786 mean_circ: 0.896136
Ran for 234050 frames Cell Events: 7803 mean_circ: 0.896051
Ran for 234550 frames Cell Events: 7824 mean_circ: 0.896017
Ran for 235550 frames Cell Events: 7860 mean_circ: 0.896108
Ran for 236550 frames Cell Events: 7896 mean_circ: 0.896073
Ran for 237050 frames Cell Events: 7905 mean_circ: 0.896059
Ran for 237550 frames Cell Events: 7918 mean_circ: 0.896053
Ran for 238050 frames Cell Events: 7936 mean_circ: 0.896071
Ran for 240550 frames Cell Events: 8015 mean_circ: 0.896093
Ran for 241050 frames Cell Events: 8027 mean_circ: 0.896063
Ran for 241550 frames Cell Events: 8049 mean_circ: 0.896057
Ran for 242050 frames Cell Events: 8059 mean_circ: 0.896102
Ran for 242550 frames Cell Events: 8081 mean_circ: 0.896174
Ran for 243050 frames Cell Events: 8097 mean_circ: 0.896204
Ran for 244550 frames Cell Events: 8150 mean_circ: 0.89621
Ran for 245550 frames Cell Events: 8189 mean_circ: 0.896144
Ran for 246050 frames Cell Events: 8199 mean_circ: 0.896092
Ran for 247050 frames Cell Events: 8248 mean_circ: 0.896073
Ran for 248050 frames Cell Events: 8294 mean_circ: 0.896175
Ran for 248550 frames Cell Events: 8310 mean_circ: 0.896166
Ran for 249050 frames Cell Events: 8319 mean_circ: 0.896196
Ran for 250050 frames Cell Events: 8350 mean_circ: 0.896259
Ran for 250550 frames Cell Events: 8370 mean_circ: 0.896244
Ran for 252050 frames Cell Events: 8423 mean_circ: 0.896177
Ran for 252550 frames Cell Events: 8437 mean_circ: 0.896154



End this measurment..



Stopping time: 2.46142e+009
****************************************
Measurement finished

Mean Circularitiy: 0.896113
Mean Area: 668.884
Mean FL-1 intensity: 2596.77
Mean FL-2 intensity: 3380.66
Mean FL-3 intensity: 0

frames processed: 252603
Total Time: 126740ms
Number of Runs (new bg img) 0
Frames collected: 253355
Skipped frames: 33665
Rescued Frames: 4059584
Samples DAQ: 63338760
Samples written: 126677410
Samples per Channel: 63338705
FPS: 1999.02

Samples per second: 499754
Expected samples/s: 500000

Events: 8459
Events per second: 66.7431
jumped back (PMT): 31
#Frames read, frequency
# 0 frames :359235
# 1 frames :126836
# 2 frames :6159
# 3 frames :2300
# 4 frames :1383
# 5 frames :764
# 6 frames :1754
# 7 frames :2057
# 8 frames :1079
# 9 frames :719
# 10 frames :1325
# 11 frames :877
# 12 frames :610
# 13 frames :365
# 14 frames :238
# 15 frames :190
# 16 frames :167
# 17 frames :152
# 18 frames :98
# 19 frames :66
# 20 frames :62
# 21 frames :40
# 22 frames :43
# 23 frames :18
# 24 frames :26
# 25 frames :17
# 26 frames :16
# 27 frames :14
# 28 frames :10
# 29 frames :8
# 30 frames :7
# 31 frames :7
# 32 frames :11
# 33 frames :7
# 34 frames :5
# 35 frames :3
# 36 frames :1
# 37 frames :4
# 38 frames :4
# 39 frames :3
# 40 frames :1
# 42 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 56 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0065
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0