Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.10.28 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ab98e2e109381111f666976aebec7005",
"name": "M1_20170613_1446_dsRNA_V3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:10:49"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8292 (max 8293) in '8292'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5971
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.5286e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.937966
Ran for 1050 frames Cell Events: 58 mean_circ: 0.937966
Ran for 1550 frames Cell Events: 78 mean_circ: 0.947403
Ran for 3050 frames Cell Events: 177 mean_circ: 0.943287
Ran for 3550 frames Cell Events: 193 mean_circ: 0.9456
Ran for 4550 frames Cell Events: 230 mean_circ: 0.945555
Ran for 5550 frames Cell Events: 272 mean_circ: 0.944892
Ran for 6050 frames Cell Events: 295 mean_circ: 0.944927
Ran for 7550 frames Cell Events: 369 mean_circ: 0.945986
Ran for 8050 frames Cell Events: 382 mean_circ: 0.94616
Ran for 8550 frames Cell Events: 406 mean_circ: 0.946579
Ran for 11550 frames Cell Events: 542 mean_circ: 0.947872
Ran for 12550 frames Cell Events: 580 mean_circ: 0.948513
Ran for 13550 frames Cell Events: 623 mean_circ: 0.94776
Ran for 14050 frames Cell Events: 644 mean_circ: 0.94776
Ran for 14550 frames Cell Events: 660 mean_circ: 0.94766
Ran for 15050 frames Cell Events: 685 mean_circ: 0.947629
Ran for 15550 frames Cell Events: 705 mean_circ: 0.947691
Ran for 16050 frames Cell Events: 720 mean_circ: 0.947763
Ran for 16550 frames Cell Events: 745 mean_circ: 0.948261
Ran for 17550 frames Cell Events: 818 mean_circ: 0.948024
Ran for 18550 frames Cell Events: 871 mean_circ: 0.948344
Ran for 19050 frames Cell Events: 889 mean_circ: 0.948333
Ran for 19550 frames Cell Events: 915 mean_circ: 0.948431
Ran for 20550 frames Cell Events: 960 mean_circ: 0.948321
Ran for 21050 frames Cell Events: 981 mean_circ: 0.948301
Ran for 21550 frames Cell Events: 1010 mean_circ: 0.948493
Ran for 22050 frames Cell Events: 1034 mean_circ: 0.948273
Ran for 23050 frames Cell Events: 1087 mean_circ: 0.948086
Ran for 23550 frames Cell Events: 1108 mean_circ: 0.948087
Ran for 24550 frames Cell Events: 1157 mean_circ: 0.948109
Ran for 25050 frames Cell Events: 1184 mean_circ: 0.947921
Ran for 27050 frames Cell Events: 1298 mean_circ: 0.9482
Ran for 27550 frames Cell Events: 1313 mean_circ: 0.948229
Ran for 28050 frames Cell Events: 1335 mean_circ: 0.948344
Ran for 28550 frames Cell Events: 1345 mean_circ: 0.948412
Ran for 29050 frames Cell Events: 1383 mean_circ: 0.948398
Ran for 31050 frames Cell Events: 1513 mean_circ: 0.948563
Ran for 32050 frames Cell Events: 1557 mean_circ: 0.948582
Ran for 32550 frames Cell Events: 1579 mean_circ: 0.948404
Ran for 36050 frames Cell Events: 1798 mean_circ: 0.947483
Ran for 37550 frames Cell Events: 1872 mean_circ: 0.947378
Ran for 38050 frames Cell Events: 1900 mean_circ: 0.947449
Ran for 39050 frames Cell Events: 1980 mean_circ: 0.947376
Ran for 40050 frames Cell Events: 2025 mean_circ: 0.947255
Ran for 42050 frames Cell Events: 2146 mean_circ: 0.947368
Ran for 43550 frames Cell Events: 2200 mean_circ: 0.947314
Ran for 44050 frames Cell Events: 2223 mean_circ: 0.947351
Ran for 44550 frames Cell Events: 2258 mean_circ: 0.947345
Ran for 45550 frames Cell Events: 2303 mean_circ: 0.947595
Ran for 46050 frames Cell Events: 2324 mean_circ: 0.94774
Ran for 46550 frames Cell Events: 2353 mean_circ: 0.947716
Ran for 48050 frames Cell Events: 2427 mean_circ: 0.947647
Ran for 50050 frames Cell Events: 2519 mean_circ: 0.947851
Ran for 51550 frames Cell Events: 2594 mean_circ: 0.947529
Ran for 52050 frames Cell Events: 2622 mean_circ: 0.947484
Ran for 53050 frames Cell Events: 2668 mean_circ: 0.947548
Ran for 53550 frames Cell Events: 2689 mean_circ: 0.947533
Ran for 54550 frames Cell Events: 2745 mean_circ: 0.947606
Ran for 55050 frames Cell Events: 2776 mean_circ: 0.947731
Ran for 56050 frames Cell Events: 2816 mean_circ: 0.947778
Ran for 56550 frames Cell Events: 2835 mean_circ: 0.947782
Ran for 57550 frames Cell Events: 2890 mean_circ: 0.947805
Ran for 58550 frames Cell Events: 2936 mean_circ: 0.947741
Ran for 59050 frames Cell Events: 2961 mean_circ: 0.947768
Ran for 60050 frames Cell Events: 3017 mean_circ: 0.947917
Ran for 61050 frames Cell Events: 3069 mean_circ: 0.947971
Ran for 62550 frames Cell Events: 3169 mean_circ: 0.947854
Ran for 63050 frames Cell Events: 3196 mean_circ: 0.947853
Ran for 63550 frames Cell Events: 3208 mean_circ: 0.947772
Ran for 64050 frames Cell Events: 3226 mean_circ: 0.947711
Ran for 64550 frames Cell Events: 3259 mean_circ: 0.947766
Ran for 65050 frames Cell Events: 3287 mean_circ: 0.947802
Ran for 67550 frames Cell Events: 3429 mean_circ: 0.94787
Ran for 68050 frames Cell Events: 3454 mean_circ: 0.947884
Ran for 68550 frames Cell Events: 3473 mean_circ: 0.947902
Ran for 69550 frames Cell Events: 3521 mean_circ: 0.947943
Ran for 71050 frames Cell Events: 3619 mean_circ: 0.94783
Ran for 71550 frames Cell Events: 3654 mean_circ: 0.947776
Ran for 74050 frames Cell Events: 3788 mean_circ: 0.947794
Ran for 77050 frames Cell Events: 3978 mean_circ: 0.947956
Ran for 78550 frames Cell Events: 4053 mean_circ: 0.947858
Ran for 79050 frames Cell Events: 4078 mean_circ: 0.947895
Ran for 81050 frames Cell Events: 4188 mean_circ: 0.947854
Ran for 81550 frames Cell Events: 4217 mean_circ: 0.947854
Ran for 85050 frames Cell Events: 4387 mean_circ: 0.948041
Ran for 85550 frames Cell Events: 4401 mean_circ: 0.948033
Ran for 86050 frames Cell Events: 4432 mean_circ: 0.94798
Ran for 86550 frames Cell Events: 4458 mean_circ: 0.947922
Ran for 88050 frames Cell Events: 4544 mean_circ: 0.947901
Ran for 88550 frames Cell Events: 4573 mean_circ: 0.947986
Ran for 89550 frames Cell Events: 4631 mean_circ: 0.948058
Ran for 90050 frames Cell Events: 4648 mean_circ: 0.948074
Ran for 91050 frames Cell Events: 4708 mean_circ: 0.948178
Ran for 91550 frames Cell Events: 4741 mean_circ: 0.948181
Ran for 93050 frames Cell Events: 4801 mean_circ: 0.948297
Ran for 95550 frames Cell Events: 4977 mean_circ: 0.948302
Ran for 96550 frames Cell Events: 5028 mean_circ: 0.948243
Ran for 97050 frames Cell Events: 5061 mean_circ: 0.948281
Ran for 97550 frames Cell Events: 5083 mean_circ: 0.9483
Ran for 98050 frames Cell Events: 5098 mean_circ: 0.948348
Ran for 99050 frames Cell Events: 5135 mean_circ: 0.948311
Ran for 99550 frames Cell Events: 5156 mean_circ: 0.948294
Ran for 106050 frames Cell Events: 5550 mean_circ: 0.948372
Ran for 107050 frames Cell Events: 5582 mean_circ: 0.948402
Ran for 107550 frames Cell Events: 5606 mean_circ: 0.948403
Ran for 108050 frames Cell Events: 5634 mean_circ: 0.948437
Ran for 108550 frames Cell Events: 5662 mean_circ: 0.94846
Ran for 109550 frames Cell Events: 5715 mean_circ: 0.948472
Ran for 111050 frames Cell Events: 5797 mean_circ: 0.948443
Ran for 112050 frames Cell Events: 5838 mean_circ: 0.948435
Ran for 114050 frames Cell Events: 5939 mean_circ: 0.948371
Ran for 114550 frames Cell Events: 5973 mean_circ: 0.948379
Ran for 115050 frames Cell Events: 6000 mean_circ: 0.948401
Ran for 115550 frames Cell Events: 6028 mean_circ: 0.948387
Ran for 116050 frames Cell Events: 6057 mean_circ: 0.948387
Ran for 117050 frames Cell Events: 6098 mean_circ: 0.948407
Ran for 117550 frames Cell Events: 6118 mean_circ: 0.948418
Ran for 118050 frames Cell Events: 6139 mean_circ: 0.948422
Ran for 118550 frames Cell Events: 6161 mean_circ: 0.948347
Ran for 119050 frames Cell Events: 6183 mean_circ: 0.948373
Ran for 120050 frames Cell Events: 6219 mean_circ: 0.94837
Ran for 120550 frames Cell Events: 6229 mean_circ: 0.948396
Ran for 121050 frames Cell Events: 6257 mean_circ: 0.948344
Ran for 121550 frames Cell Events: 6285 mean_circ: 0.948338
Ran for 122550 frames Cell Events: 6353 mean_circ: 0.948383
Ran for 124050 frames Cell Events: 6443 mean_circ: 0.948415
Ran for 124550 frames Cell Events: 6474 mean_circ: 0.948412
Ran for 125050 frames Cell Events: 6493 mean_circ: 0.948432
Ran for 126050 frames Cell Events: 6537 mean_circ: 0.948475
Ran for 128550 frames Cell Events: 6670 mean_circ: 0.948374
Ran for 129050 frames Cell Events: 6699 mean_circ: 0.948397
Ran for 130550 frames Cell Events: 6779 mean_circ: 0.948396
Ran for 132050 frames Cell Events: 6851 mean_circ: 0.948311
Ran for 133550 frames Cell Events: 6921 mean_circ: 0.948347
Ran for 135050 frames Cell Events: 6985 mean_circ: 0.948357
Ran for 136050 frames Cell Events: 7029 mean_circ: 0.948314
Ran for 137550 frames Cell Events: 7106 mean_circ: 0.94833
Ran for 139050 frames Cell Events: 7193 mean_circ: 0.9483
Ran for 141050 frames Cell Events: 7285 mean_circ: 0.948286
Ran for 141550 frames Cell Events: 7309 mean_circ: 0.948235
Ran for 142550 frames Cell Events: 7365 mean_circ: 0.948202
Ran for 143550 frames Cell Events: 7424 mean_circ: 0.948236
Ran for 144550 frames Cell Events: 7472 mean_circ: 0.948298
Ran for 145550 frames Cell Events: 7523 mean_circ: 0.948294
Ran for 147550 frames Cell Events: 7620 mean_circ: 0.948283
Ran for 148050 frames Cell Events: 7640 mean_circ: 0.94829
Ran for 149050 frames Cell Events: 7694 mean_circ: 0.948244
Ran for 150050 frames Cell Events: 7740 mean_circ: 0.948291
Ran for 152050 frames Cell Events: 7836 mean_circ: 0.948252
Ran for 152550 frames Cell Events: 7862 mean_circ: 0.94826
Ran for 155050 frames Cell Events: 8023 mean_circ: 0.948295
Ran for 155550 frames Cell Events: 8045 mean_circ: 0.948296
Ran for 156550 frames Cell Events: 8111 mean_circ: 0.948299
Ran for 157050 frames Cell Events: 8132 mean_circ: 0.948301
Ran for 159550 frames Cell Events: 8265 mean_circ: 0.948322



End this measurment..



Stopping time: 2.52868e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948315
Mean Area: 608.35
Mean FL-1 intensity: 3374.62
Mean FL-2 intensity: 3450.83
Mean FL-3 intensity: 0

frames processed: 159581
Total Time: 80039.6ms
Number of Runs (new bg img) 0
Frames collected: 159938
Skipped frames: 11372
Rescued Frames: 1599736
Samples DAQ: 39986100
Samples written: 79969570
Samples per Channel: 39984785
FPS: 1998.24

Samples per second: 499562
Expected samples/s: 500000

Events: 8293
Events per second: 103.611
jumped back (PMT): 4276
#Frames read, frequency
# 0 frames :243724
# 1 frames :61763
# 2 frames :3775
# 3 frames :1287
# 4 frames :765
# 5 frames :381
# 6 frames :1422
# 7 frames :1678
# 8 frames :948
# 9 frames :565
# 10 frames :651
# 11 frames :761
# 12 frames :371
# 13 frames :232
# 14 frames :208
# 15 frames :206
# 16 frames :152
# 17 frames :148
# 18 frames :100
# 19 frames :67
# 20 frames :55
# 21 frames :68
# 22 frames :46
# 23 frames :53
# 24 frames :35
# 25 frames :36
# 26 frames :39
# 27 frames :20
# 28 frames :13
# 29 frames :16
# 30 frames :10
# 31 frames :13
# 32 frames :11
# 33 frames :11
# 34 frames :9
# 35 frames :5
# 36 frames :11
# 37 frames :7
# 38 frames :2
# 39 frames :3
# 40 frames :1
# 41 frames :2
# 42 frames :1
# 45 frames :1
# 46 frames :2
# 47 frames :2
# 50 frames :2
# 52 frames :2
# 53 frames :2
# 62 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5971
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0