Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.10.56 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "48c40727692a698783ebb472b9f33593",
"name": "M1_20170613_1449_dsRNA_V3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:11:21"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6003
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.52876e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 25 mean_circ: 0.933368
Ran for 1550 frames Cell Events: 96 mean_circ: 0.942203
Ran for 2050 frames Cell Events: 123 mean_circ: 0.940567
Ran for 2550 frames Cell Events: 157 mean_circ: 0.939683
Ran for 4050 frames Cell Events: 220 mean_circ: 0.940714
Ran for 4550 frames Cell Events: 246 mean_circ: 0.940975
Ran for 5050 frames Cell Events: 276 mean_circ: 0.940918
Ran for 5550 frames Cell Events: 297 mean_circ: 0.94094
Ran for 6550 frames Cell Events: 355 mean_circ: 0.939296
Ran for 7050 frames Cell Events: 377 mean_circ: 0.939821
Ran for 8050 frames Cell Events: 421 mean_circ: 0.939544
Ran for 8550 frames Cell Events: 446 mean_circ: 0.939526
Ran for 9550 frames Cell Events: 499 mean_circ: 0.938303
Ran for 10050 frames Cell Events: 530 mean_circ: 0.938546
Ran for 11550 frames Cell Events: 630 mean_circ: 0.939854
Ran for 12050 frames Cell Events: 654 mean_circ: 0.939504
Ran for 12550 frames Cell Events: 680 mean_circ: 0.939889
Ran for 13550 frames Cell Events: 727 mean_circ: 0.939211
Ran for 14550 frames Cell Events: 785 mean_circ: 0.939301
Ran for 15050 frames Cell Events: 806 mean_circ: 0.939282
Ran for 15550 frames Cell Events: 840 mean_circ: 0.939355
Ran for 16050 frames Cell Events: 864 mean_circ: 0.939503
Ran for 17550 frames Cell Events: 910 mean_circ: 0.93958
Ran for 19050 frames Cell Events: 993 mean_circ: 0.939615
Ran for 19550 frames Cell Events: 1014 mean_circ: 0.940058
Ran for 20550 frames Cell Events: 1066 mean_circ: 0.940008
Ran for 22550 frames Cell Events: 1153 mean_circ: 0.940685
Ran for 23550 frames Cell Events: 1204 mean_circ: 0.940435
Ran for 29050 frames Cell Events: 1588 mean_circ: 0.940013
Ran for 30550 frames Cell Events: 1686 mean_circ: 0.939246
Ran for 33550 frames Cell Events: 1873 mean_circ: 0.939113
Ran for 34050 frames Cell Events: 1902 mean_circ: 0.939214
Ran for 36050 frames Cell Events: 2011 mean_circ: 0.939184
Ran for 36550 frames Cell Events: 2036 mean_circ: 0.939208
Ran for 37050 frames Cell Events: 2055 mean_circ: 0.939232
Ran for 37550 frames Cell Events: 2079 mean_circ: 0.93901
Ran for 38550 frames Cell Events: 2156 mean_circ: 0.938868
Ran for 40550 frames Cell Events: 2306 mean_circ: 0.938862
Ran for 41050 frames Cell Events: 2339 mean_circ: 0.938881
Ran for 41550 frames Cell Events: 2371 mean_circ: 0.938881
Ran for 43050 frames Cell Events: 2459 mean_circ: 0.938625
Ran for 43550 frames Cell Events: 2476 mean_circ: 0.938688
Ran for 44050 frames Cell Events: 2493 mean_circ: 0.938632
Ran for 44550 frames Cell Events: 2516 mean_circ: 0.938654
Ran for 45050 frames Cell Events: 2541 mean_circ: 0.938715
Ran for 46050 frames Cell Events: 2604 mean_circ: 0.938737
Ran for 46550 frames Cell Events: 2634 mean_circ: 0.938699
Ran for 47050 frames Cell Events: 2670 mean_circ: 0.938704
Ran for 49550 frames Cell Events: 2843 mean_circ: 0.938504
Ran for 51050 frames Cell Events: 2932 mean_circ: 0.93845
Ran for 51550 frames Cell Events: 2970 mean_circ: 0.93848
Ran for 52050 frames Cell Events: 2998 mean_circ: 0.938601
Ran for 52550 frames Cell Events: 3034 mean_circ: 0.938688
Ran for 54550 frames Cell Events: 3144 mean_circ: 0.938559
Ran for 57550 frames Cell Events: 3339 mean_circ: 0.938553
Ran for 59050 frames Cell Events: 3403 mean_circ: 0.938444
Ran for 62050 frames Cell Events: 3581 mean_circ: 0.938651
Ran for 63550 frames Cell Events: 3667 mean_circ: 0.938592
Ran for 65050 frames Cell Events: 3758 mean_circ: 0.938512
Ran for 66050 frames Cell Events: 3825 mean_circ: 0.938482
Ran for 66550 frames Cell Events: 3856 mean_circ: 0.938383
Ran for 67050 frames Cell Events: 3884 mean_circ: 0.938436
Ran for 67550 frames Cell Events: 3911 mean_circ: 0.93843
Ran for 68550 frames Cell Events: 3973 mean_circ: 0.938517
Ran for 69050 frames Cell Events: 3989 mean_circ: 0.93858
Ran for 70050 frames Cell Events: 4049 mean_circ: 0.938537
Ran for 70550 frames Cell Events: 4075 mean_circ: 0.938613
Ran for 74550 frames Cell Events: 4323 mean_circ: 0.938592
Ran for 75050 frames Cell Events: 4347 mean_circ: 0.938555
Ran for 76050 frames Cell Events: 4426 mean_circ: 0.938547
Ran for 76550 frames Cell Events: 4461 mean_circ: 0.938573
Ran for 77050 frames Cell Events: 4502 mean_circ: 0.938491
Ran for 77550 frames Cell Events: 4534 mean_circ: 0.938483
Ran for 78550 frames Cell Events: 4594 mean_circ: 0.938485
Ran for 79050 frames Cell Events: 4612 mean_circ: 0.938487
Ran for 81550 frames Cell Events: 4773 mean_circ: 0.938452
Ran for 83050 frames Cell Events: 4857 mean_circ: 0.938537
Ran for 83550 frames Cell Events: 4897 mean_circ: 0.93863
Ran for 84050 frames Cell Events: 4929 mean_circ: 0.938663
Ran for 89050 frames Cell Events: 5243 mean_circ: 0.938592
Ran for 89550 frames Cell Events: 5278 mean_circ: 0.938604
Ran for 90550 frames Cell Events: 5314 mean_circ: 0.93862
Ran for 91550 frames Cell Events: 5365 mean_circ: 0.938696
Ran for 92050 frames Cell Events: 5394 mean_circ: 0.938756
Ran for 93050 frames Cell Events: 5438 mean_circ: 0.938698
Ran for 93550 frames Cell Events: 5460 mean_circ: 0.938714
Ran for 94050 frames Cell Events: 5472 mean_circ: 0.938714
Ran for 94550 frames Cell Events: 5497 mean_circ: 0.938696
Ran for 96550 frames Cell Events: 5626 mean_circ: 0.938769
Ran for 97050 frames Cell Events: 5650 mean_circ: 0.938781
Ran for 98050 frames Cell Events: 5698 mean_circ: 0.938833
Ran for 99050 frames Cell Events: 5775 mean_circ: 0.938775
Ran for 99550 frames Cell Events: 5813 mean_circ: 0.93873
Ran for 100050 frames Cell Events: 5838 mean_circ: 0.93869
Ran for 100550 frames Cell Events: 5860 mean_circ: 0.938727
Ran for 102050 frames Cell Events: 5954 mean_circ: 0.938706
Ran for 103550 frames Cell Events: 6037 mean_circ: 0.938704
Ran for 105050 frames Cell Events: 6133 mean_circ: 0.938694
Ran for 105550 frames Cell Events: 6159 mean_circ: 0.938694
Ran for 109050 frames Cell Events: 6373 mean_circ: 0.938666
Ran for 110050 frames Cell Events: 6444 mean_circ: 0.938589
Ran for 111550 frames Cell Events: 6528 mean_circ: 0.938643
Ran for 113550 frames Cell Events: 6673 mean_circ: 0.938688
Ran for 114550 frames Cell Events: 6753 mean_circ: 0.938598
Ran for 115550 frames Cell Events: 6824 mean_circ: 0.938536
Ran for 119550 frames Cell Events: 7061 mean_circ: 0.938433
Ran for 120050 frames Cell Events: 7090 mean_circ: 0.93844
Ran for 121050 frames Cell Events: 7161 mean_circ: 0.93847
Ran for 121550 frames Cell Events: 7184 mean_circ: 0.938487
Ran for 125050 frames Cell Events: 7422 mean_circ: 0.938402
Ran for 125550 frames Cell Events: 7465 mean_circ: 0.938419
Ran for 128550 frames Cell Events: 7656 mean_circ: 0.938357
Ran for 129050 frames Cell Events: 7687 mean_circ: 0.938357
Ran for 129550 frames Cell Events: 7718 mean_circ: 0.938372
Ran for 130550 frames Cell Events: 7775 mean_circ: 0.938374
Ran for 132050 frames Cell Events: 7866 mean_circ: 0.938372
Ran for 133050 frames Cell Events: 7924 mean_circ: 0.938354
Ran for 135550 frames Cell Events: 8076 mean_circ: 0.938315
Ran for 136050 frames Cell Events: 8090 mean_circ: 0.938328
Ran for 136550 frames Cell Events: 8115 mean_circ: 0.938337
Ran for 139050 frames Cell Events: 8242 mean_circ: 0.93833
Ran for 140550 frames Cell Events: 8356 mean_circ: 0.938338
Ran for 142550 frames Cell Events: 8470 mean_circ: 0.93837
Ran for 143050 frames Cell Events: 8497 mean_circ: 0.938414
Ran for 143550 frames Cell Events: 8523 mean_circ: 0.938413
Ran for 144050 frames Cell Events: 8542 mean_circ: 0.938436
Ran for 146050 frames Cell Events: 8655 mean_circ: 0.938348
Ran for 146550 frames Cell Events: 8689 mean_circ: 0.938328
Ran for 149050 frames Cell Events: 8828 mean_circ: 0.938339
Ran for 151550 frames Cell Events: 8976 mean_circ: 0.938366
Ran for 152050 frames Cell Events: 9000 mean_circ: 0.938343
Ran for 152550 frames Cell Events: 9020 mean_circ: 0.938355
Ran for 153050 frames Cell Events: 9043 mean_circ: 0.938391
Ran for 153550 frames Cell Events: 9068 mean_circ: 0.938382
Ran for 154050 frames Cell Events: 9086 mean_circ: 0.938394
Ran for 155050 frames Cell Events: 9146 mean_circ: 0.938422
Ran for 155550 frames Cell Events: 9179 mean_circ: 0.938402
Ran for 156050 frames Cell Events: 9211 mean_circ: 0.938425
Ran for 157050 frames Cell Events: 9265 mean_circ: 0.938443
Ran for 159050 frames Cell Events: 9416 mean_circ: 0.938377
Ran for 159550 frames Cell Events: 9441 mean_circ: 0.938362
Ran for 160550 frames Cell Events: 9511 mean_circ: 0.938324
Ran for 162050 frames Cell Events: 9596 mean_circ: 0.938323
Ran for 163050 frames Cell Events: 9673 mean_circ: 0.938342
Ran for 164050 frames Cell Events: 9730 mean_circ: 0.938296
Ran for 166050 frames Cell Events: 9824 mean_circ: 0.938359
Ran for 167550 frames Cell Events: 9913 mean_circ: 0.938328



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 2.52885e+009
****************************************
Measurement finished

Mean Circularitiy: 0.938377
Mean Area: 603.454
Mean FL-1 intensity: 3174.35
Mean FL-2 intensity: 3751.18
Mean FL-3 intensity: 0

frames processed: 166567
Total Time: 84408.9ms
Number of Runs (new bg img) 0
Frames collected: 168683
Skipped frames: 13488
Rescued Frames: 1694702
Samples DAQ: 42170880
Samples written: 84341272
Samples per Channel: 42170636
FPS: 1998.4

Samples per second: 499599
Expected samples/s: 500000

Events: 10002
Events per second: 118.495
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :265081
# 1 frames :56475
# 2 frames :3799
# 3 frames :1170
# 4 frames :794
# 5 frames :366
# 6 frames :2092
# 7 frames :1303
# 8 frames :1398
# 9 frames :840
# 10 frames :601
# 11 frames :405
# 12 frames :303
# 13 frames :199
# 14 frames :287
# 15 frames :253
# 16 frames :220
# 17 frames :179
# 18 frames :126
# 19 frames :94
# 20 frames :80
# 21 frames :72
# 22 frames :86
# 23 frames :62
# 24 frames :54
# 25 frames :34
# 26 frames :46
# 27 frames :34
# 28 frames :32
# 29 frames :28
# 30 frames :19
# 31 frames :15
# 32 frames :20
# 33 frames :13
# 34 frames :12
# 35 frames :13
# 36 frames :21
# 37 frames :7
# 38 frames :6
# 39 frames :6
# 40 frames :7
# 41 frames :3
# 42 frames :6
# 43 frames :4
# 44 frames :6
# 45 frames :4
# 46 frames :4
# 48 frames :2
# 50 frames :1
# 51 frames :5
# 53 frames :4
# 54 frames :1
# 56 frames :1
# 57 frames :3
# 62 frames :1
# 63 frames :1
# 70 frames :1
# 74 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6003
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0