Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.12.49 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c84ee6e1bc7cf0a0666ee875f80f690a",
"name": "M1_20170613_1511_dsRNA_W2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:13:10"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8199 (max 8201) in '8199'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5926
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53007e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.949354
Ran for 550 frames Cell Events: 31 mean_circ: 0.883608
Ran for 1550 frames Cell Events: 84 mean_circ: 0.907751
Ran for 2550 frames Cell Events: 133 mean_circ: 0.92025
Ran for 3050 frames Cell Events: 164 mean_circ: 0.920148
Ran for 3550 frames Cell Events: 188 mean_circ: 0.922239
Ran for 4050 frames Cell Events: 213 mean_circ: 0.923065
Ran for 5050 frames Cell Events: 259 mean_circ: 0.928189
Ran for 6050 frames Cell Events: 318 mean_circ: 0.931268
Ran for 8050 frames Cell Events: 428 mean_circ: 0.933627
Ran for 8550 frames Cell Events: 451 mean_circ: 0.933895
Ran for 9050 frames Cell Events: 478 mean_circ: 0.934255
Ran for 9550 frames Cell Events: 511 mean_circ: 0.934409
Ran for 10050 frames Cell Events: 533 mean_circ: 0.934748
Ran for 10550 frames Cell Events: 555 mean_circ: 0.935004
Ran for 11050 frames Cell Events: 591 mean_circ: 0.935289
Ran for 11550 frames Cell Events: 623 mean_circ: 0.935331
Ran for 12050 frames Cell Events: 643 mean_circ: 0.93546
Ran for 12550 frames Cell Events: 675 mean_circ: 0.935771
Ran for 13050 frames Cell Events: 697 mean_circ: 0.936065
Ran for 14050 frames Cell Events: 741 mean_circ: 0.935966
Ran for 15050 frames Cell Events: 799 mean_circ: 0.935987
Ran for 15550 frames Cell Events: 811 mean_circ: 0.936012
Ran for 16050 frames Cell Events: 832 mean_circ: 0.936112
Ran for 16550 frames Cell Events: 860 mean_circ: 0.936443
Ran for 18550 frames Cell Events: 948 mean_circ: 0.936546
Ran for 19550 frames Cell Events: 1013 mean_circ: 0.936454
Ran for 20050 frames Cell Events: 1036 mean_circ: 0.936618
Ran for 21050 frames Cell Events: 1086 mean_circ: 0.936778
Ran for 21550 frames Cell Events: 1119 mean_circ: 0.937217
Ran for 22050 frames Cell Events: 1147 mean_circ: 0.937211
Ran for 23050 frames Cell Events: 1194 mean_circ: 0.936837
Ran for 24050 frames Cell Events: 1240 mean_circ: 0.936991
Ran for 24550 frames Cell Events: 1264 mean_circ: 0.937146
Ran for 25050 frames Cell Events: 1293 mean_circ: 0.937043
Ran for 25550 frames Cell Events: 1324 mean_circ: 0.936885
Ran for 26050 frames Cell Events: 1349 mean_circ: 0.93684
Ran for 27050 frames Cell Events: 1389 mean_circ: 0.936632
Ran for 27550 frames Cell Events: 1410 mean_circ: 0.93679
Ran for 30550 frames Cell Events: 1572 mean_circ: 0.936802
Ran for 31050 frames Cell Events: 1603 mean_circ: 0.936945
Ran for 32050 frames Cell Events: 1646 mean_circ: 0.936778
Ran for 33050 frames Cell Events: 1707 mean_circ: 0.936866
Ran for 34550 frames Cell Events: 1766 mean_circ: 0.936746
Ran for 35050 frames Cell Events: 1788 mean_circ: 0.936907
Ran for 35550 frames Cell Events: 1820 mean_circ: 0.936796
Ran for 37050 frames Cell Events: 1881 mean_circ: 0.93714
Ran for 38550 frames Cell Events: 1947 mean_circ: 0.937177
Ran for 39550 frames Cell Events: 2001 mean_circ: 0.937243
Ran for 40550 frames Cell Events: 2048 mean_circ: 0.937473
Ran for 41050 frames Cell Events: 2067 mean_circ: 0.937604
Ran for 41550 frames Cell Events: 2091 mean_circ: 0.9375
Ran for 43550 frames Cell Events: 2177 mean_circ: 0.93773
Ran for 45050 frames Cell Events: 2219 mean_circ: 0.937574
Ran for 45550 frames Cell Events: 2240 mean_circ: 0.937674
Ran for 46050 frames Cell Events: 2256 mean_circ: 0.937728
Ran for 46550 frames Cell Events: 2285 mean_circ: 0.937794
Ran for 47050 frames Cell Events: 2303 mean_circ: 0.937798
Ran for 47550 frames Cell Events: 2336 mean_circ: 0.937801
Ran for 48050 frames Cell Events: 2376 mean_circ: 0.937769
Ran for 48550 frames Cell Events: 2391 mean_circ: 0.937815
Ran for 49050 frames Cell Events: 2409 mean_circ: 0.937804
Ran for 49550 frames Cell Events: 2434 mean_circ: 0.937806
Ran for 50550 frames Cell Events: 2481 mean_circ: 0.937706
Ran for 51050 frames Cell Events: 2511 mean_circ: 0.937756
Ran for 51550 frames Cell Events: 2536 mean_circ: 0.937741
Ran for 53050 frames Cell Events: 2614 mean_circ: 0.937782
Ran for 53550 frames Cell Events: 2628 mean_circ: 0.937715
Ran for 54550 frames Cell Events: 2681 mean_circ: 0.937535
Ran for 55050 frames Cell Events: 2708 mean_circ: 0.937554
Ran for 55550 frames Cell Events: 2733 mean_circ: 0.937618
Ran for 56050 frames Cell Events: 2757 mean_circ: 0.937602
Ran for 56550 frames Cell Events: 2776 mean_circ: 0.937556
Ran for 57050 frames Cell Events: 2804 mean_circ: 0.937536
Ran for 57550 frames Cell Events: 2824 mean_circ: 0.937524
Ran for 58050 frames Cell Events: 2840 mean_circ: 0.93755
Ran for 58550 frames Cell Events: 2876 mean_circ: 0.937486
Ran for 59050 frames Cell Events: 2907 mean_circ: 0.937334
Ran for 60550 frames Cell Events: 2997 mean_circ: 0.937565
Ran for 62050 frames Cell Events: 3092 mean_circ: 0.937688
Ran for 62550 frames Cell Events: 3123 mean_circ: 0.937835
Ran for 63050 frames Cell Events: 3155 mean_circ: 0.937846
Ran for 64050 frames Cell Events: 3190 mean_circ: 0.937732
Ran for 65050 frames Cell Events: 3237 mean_circ: 0.937687
Ran for 66050 frames Cell Events: 3277 mean_circ: 0.937659
Ran for 68550 frames Cell Events: 3427 mean_circ: 0.937681
Ran for 69050 frames Cell Events: 3456 mean_circ: 0.937698
Ran for 69550 frames Cell Events: 3485 mean_circ: 0.937719
Ran for 70550 frames Cell Events: 3528 mean_circ: 0.937671
Ran for 71050 frames Cell Events: 3548 mean_circ: 0.937691
Ran for 72050 frames Cell Events: 3608 mean_circ: 0.937664
Ran for 72550 frames Cell Events: 3636 mean_circ: 0.937741
Ran for 73050 frames Cell Events: 3654 mean_circ: 0.937696
Ran for 73550 frames Cell Events: 3684 mean_circ: 0.937614
Ran for 74050 frames Cell Events: 3708 mean_circ: 0.937658
Ran for 74550 frames Cell Events: 3730 mean_circ: 0.937664
Ran for 75050 frames Cell Events: 3754 mean_circ: 0.937706
Ran for 75550 frames Cell Events: 3789 mean_circ: 0.937696
Ran for 76550 frames Cell Events: 3845 mean_circ: 0.937708
Ran for 77050 frames Cell Events: 3876 mean_circ: 0.937766
Ran for 78050 frames Cell Events: 3928 mean_circ: 0.93769
Ran for 80550 frames Cell Events: 4091 mean_circ: 0.937944
Ran for 82550 frames Cell Events: 4188 mean_circ: 0.937704
Ran for 83050 frames Cell Events: 4219 mean_circ: 0.937721
Ran for 85050 frames Cell Events: 4348 mean_circ: 0.937564
Ran for 85550 frames Cell Events: 4378 mean_circ: 0.937577
Ran for 86050 frames Cell Events: 4411 mean_circ: 0.937623
Ran for 87050 frames Cell Events: 4470 mean_circ: 0.937625
Ran for 88050 frames Cell Events: 4528 mean_circ: 0.937677
Ran for 88550 frames Cell Events: 4557 mean_circ: 0.93769
Ran for 89050 frames Cell Events: 4584 mean_circ: 0.937731
Ran for 90050 frames Cell Events: 4636 mean_circ: 0.93775
Ran for 90550 frames Cell Events: 4651 mean_circ: 0.937751
Ran for 91050 frames Cell Events: 4666 mean_circ: 0.937743
Ran for 91550 frames Cell Events: 4694 mean_circ: 0.93771
Ran for 94050 frames Cell Events: 4834 mean_circ: 0.937726
Ran for 96050 frames Cell Events: 4941 mean_circ: 0.937608
Ran for 97050 frames Cell Events: 4991 mean_circ: 0.937732
Ran for 97550 frames Cell Events: 5017 mean_circ: 0.937792
Ran for 98550 frames Cell Events: 5075 mean_circ: 0.937699
Ran for 99050 frames Cell Events: 5105 mean_circ: 0.93767
Ran for 99550 frames Cell Events: 5133 mean_circ: 0.937653
Ran for 100550 frames Cell Events: 5178 mean_circ: 0.937677
Ran for 101550 frames Cell Events: 5236 mean_circ: 0.937683
Ran for 102050 frames Cell Events: 5266 mean_circ: 0.937686
Ran for 102550 frames Cell Events: 5283 mean_circ: 0.937736
Ran for 103050 frames Cell Events: 5309 mean_circ: 0.937729
Ran for 103550 frames Cell Events: 5338 mean_circ: 0.937746
Ran for 104550 frames Cell Events: 5380 mean_circ: 0.937746
Ran for 105050 frames Cell Events: 5400 mean_circ: 0.937725
Ran for 105550 frames Cell Events: 5426 mean_circ: 0.937654
Ran for 106050 frames Cell Events: 5452 mean_circ: 0.937663
Ran for 106550 frames Cell Events: 5472 mean_circ: 0.937643
Ran for 108050 frames Cell Events: 5562 mean_circ: 0.937443
Ran for 108550 frames Cell Events: 5587 mean_circ: 0.937476
Ran for 109550 frames Cell Events: 5637 mean_circ: 0.937435
Ran for 110550 frames Cell Events: 5703 mean_circ: 0.937493
Ran for 113050 frames Cell Events: 5860 mean_circ: 0.937554
Ran for 113550 frames Cell Events: 5895 mean_circ: 0.937599
Ran for 114050 frames Cell Events: 5923 mean_circ: 0.937571
Ran for 116550 frames Cell Events: 6061 mean_circ: 0.937678
Ran for 117050 frames Cell Events: 6087 mean_circ: 0.937704
Ran for 118550 frames Cell Events: 6170 mean_circ: 0.93765
Ran for 120550 frames Cell Events: 6276 mean_circ: 0.93774
Ran for 121050 frames Cell Events: 6301 mean_circ: 0.937718
Ran for 121550 frames Cell Events: 6337 mean_circ: 0.93768
Ran for 122550 frames Cell Events: 6417 mean_circ: 0.937685
Ran for 123050 frames Cell Events: 6442 mean_circ: 0.937695
Ran for 124050 frames Cell Events: 6500 mean_circ: 0.937621
Ran for 125050 frames Cell Events: 6571 mean_circ: 0.937618
Ran for 127050 frames Cell Events: 6680 mean_circ: 0.937626
Ran for 128050 frames Cell Events: 6739 mean_circ: 0.937634
Ran for 129050 frames Cell Events: 6799 mean_circ: 0.937592
Ran for 132050 frames Cell Events: 6959 mean_circ: 0.937582
Ran for 134050 frames Cell Events: 7057 mean_circ: 0.937672
Ran for 135050 frames Cell Events: 7109 mean_circ: 0.937635
Ran for 135550 frames Cell Events: 7118 mean_circ: 0.937656
Ran for 136050 frames Cell Events: 7139 mean_circ: 0.937592
Ran for 137550 frames Cell Events: 7244 mean_circ: 0.937472
Ran for 138550 frames Cell Events: 7287 mean_circ: 0.937423
Ran for 139050 frames Cell Events: 7311 mean_circ: 0.937447
Ran for 139550 frames Cell Events: 7329 mean_circ: 0.937442
Ran for 140050 frames Cell Events: 7352 mean_circ: 0.937414
Ran for 140550 frames Cell Events: 7386 mean_circ: 0.937446
Ran for 141050 frames Cell Events: 7423 mean_circ: 0.937444
Ran for 141550 frames Cell Events: 7451 mean_circ: 0.937346
Ran for 142550 frames Cell Events: 7489 mean_circ: 0.937401
Ran for 145050 frames Cell Events: 7645 mean_circ: 0.937334
Ran for 145550 frames Cell Events: 7675 mean_circ: 0.937339
Ran for 146050 frames Cell Events: 7702 mean_circ: 0.937314
Ran for 147050 frames Cell Events: 7780 mean_circ: 0.937399
Ran for 147550 frames Cell Events: 7802 mean_circ: 0.937442
Ran for 148050 frames Cell Events: 7831 mean_circ: 0.937442
Ran for 148550 frames Cell Events: 7859 mean_circ: 0.937453
Ran for 149050 frames Cell Events: 7889 mean_circ: 0.937436
Ran for 149550 frames Cell Events: 7907 mean_circ: 0.93744
Ran for 150050 frames Cell Events: 7930 mean_circ: 0.937434
Ran for 153050 frames Cell Events: 8113 mean_circ: 0.93752
Ran for 154550 frames Cell Events: 8200 mean_circ: 0.937626



End this measurment..



Stopping time: 2.53015e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937632
Mean Area: 592.33
Mean FL-1 intensity: 3039.55
Mean FL-2 intensity: 3674.01
Mean FL-3 intensity: 0

frames processed: 152890
Total Time: 77342.4ms
Number of Runs (new bg img) 0
Frames collected: 154560
Skipped frames: 16597
Rescued Frames: 2019263
Samples DAQ: 38641328
Samples written: 77279778
Samples per Channel: 38639889
FPS: 1998.39

Samples per second: 499595
Expected samples/s: 500000

Events: 8201
Events per second: 106.035
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :232791
# 1 frames :62068
# 2 frames :4083
# 3 frames :1115
# 4 frames :718
# 5 frames :265
# 6 frames :2343
# 7 frames :1329
# 8 frames :1284
# 9 frames :711
# 10 frames :483
# 11 frames :339
# 12 frames :287
# 13 frames :176
# 14 frames :189
# 15 frames :209
# 16 frames :153
# 17 frames :100
# 18 frames :90
# 19 frames :41
# 20 frames :44
# 21 frames :36
# 22 frames :45
# 23 frames :31
# 24 frames :39
# 25 frames :25
# 26 frames :10
# 27 frames :13
# 28 frames :9
# 29 frames :10
# 30 frames :9
# 31 frames :12
# 32 frames :6
# 33 frames :11
# 34 frames :6
# 35 frames :1
# 36 frames :5
# 37 frames :2
# 38 frames :3
# 40 frames :3
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :3
# 46 frames :1
# 47 frames :1
# 51 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5926
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0