Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.13.45 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "379b5dc4074242049cd32a662cc0554d",
"name": "M1_20170613_1521_dsRNA_V6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:14:08"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9146 (max 9147) in '9146'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7149
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53068e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 29 mean_circ: 0.947237
Ran for 1050 frames Cell Events: 54 mean_circ: 0.947237
Ran for 1550 frames Cell Events: 71 mean_circ: 0.945926
Ran for 2050 frames Cell Events: 104 mean_circ: 0.943887
Ran for 3050 frames Cell Events: 169 mean_circ: 0.938949
Ran for 4050 frames Cell Events: 227 mean_circ: 0.941452
Ran for 4550 frames Cell Events: 249 mean_circ: 0.94127
Ran for 6550 frames Cell Events: 359 mean_circ: 0.942512
Ran for 8550 frames Cell Events: 458 mean_circ: 0.941507
Ran for 9050 frames Cell Events: 492 mean_circ: 0.941324
Ran for 10050 frames Cell Events: 549 mean_circ: 0.941
Ran for 10550 frames Cell Events: 573 mean_circ: 0.941066
Ran for 12050 frames Cell Events: 667 mean_circ: 0.940976
Ran for 12550 frames Cell Events: 679 mean_circ: 0.941013
Ran for 13050 frames Cell Events: 702 mean_circ: 0.940943
Ran for 13550 frames Cell Events: 733 mean_circ: 0.941225
Ran for 14050 frames Cell Events: 758 mean_circ: 0.94115
Ran for 14550 frames Cell Events: 781 mean_circ: 0.941214
Ran for 15050 frames Cell Events: 812 mean_circ: 0.941484
Ran for 15550 frames Cell Events: 841 mean_circ: 0.941122
Ran for 17050 frames Cell Events: 931 mean_circ: 0.941251
Ran for 19050 frames Cell Events: 1082 mean_circ: 0.940825
Ran for 20050 frames Cell Events: 1102 mean_circ: 0.940538
Ran for 21550 frames Cell Events: 1201 mean_circ: 0.940564
Ran for 22050 frames Cell Events: 1220 mean_circ: 0.940346
Ran for 23550 frames Cell Events: 1289 mean_circ: 0.94074
Ran for 24050 frames Cell Events: 1305 mean_circ: 0.940621
Ran for 27050 frames Cell Events: 1456 mean_circ: 0.941327
Ran for 27550 frames Cell Events: 1476 mean_circ: 0.941343
Ran for 28050 frames Cell Events: 1511 mean_circ: 0.940866
Ran for 29050 frames Cell Events: 1563 mean_circ: 0.94061
Ran for 30550 frames Cell Events: 1643 mean_circ: 0.940353
Ran for 31050 frames Cell Events: 1658 mean_circ: 0.940325
Ran for 31550 frames Cell Events: 1672 mean_circ: 0.940224
Ran for 32050 frames Cell Events: 1697 mean_circ: 0.940263
Ran for 32550 frames Cell Events: 1718 mean_circ: 0.94043
Ran for 33050 frames Cell Events: 1738 mean_circ: 0.940447
Ran for 33550 frames Cell Events: 1775 mean_circ: 0.940468
Ran for 34050 frames Cell Events: 1811 mean_circ: 0.940445
Ran for 34550 frames Cell Events: 1848 mean_circ: 0.940343
Ran for 36050 frames Cell Events: 1942 mean_circ: 0.940245
Ran for 36550 frames Cell Events: 1970 mean_circ: 0.940333
Ran for 37550 frames Cell Events: 2010 mean_circ: 0.94033
Ran for 38550 frames Cell Events: 2069 mean_circ: 0.940354
Ran for 39550 frames Cell Events: 2122 mean_circ: 0.940416
Ran for 41050 frames Cell Events: 2216 mean_circ: 0.940276
Ran for 42050 frames Cell Events: 2267 mean_circ: 0.940272
Ran for 42550 frames Cell Events: 2304 mean_circ: 0.940278
Ran for 43550 frames Cell Events: 2347 mean_circ: 0.939951
Ran for 44050 frames Cell Events: 2366 mean_circ: 0.939881
Ran for 46050 frames Cell Events: 2477 mean_circ: 0.939685
Ran for 47050 frames Cell Events: 2533 mean_circ: 0.939632
Ran for 48050 frames Cell Events: 2600 mean_circ: 0.939765
Ran for 49050 frames Cell Events: 2650 mean_circ: 0.939597
Ran for 52550 frames Cell Events: 2812 mean_circ: 0.939558
Ran for 54050 frames Cell Events: 2895 mean_circ: 0.93963
Ran for 55050 frames Cell Events: 2959 mean_circ: 0.939682
Ran for 55550 frames Cell Events: 2983 mean_circ: 0.939539
Ran for 56050 frames Cell Events: 3011 mean_circ: 0.939602
Ran for 56550 frames Cell Events: 3031 mean_circ: 0.939586
Ran for 57050 frames Cell Events: 3052 mean_circ: 0.939628
Ran for 58050 frames Cell Events: 3101 mean_circ: 0.939714
Ran for 60050 frames Cell Events: 3211 mean_circ: 0.939462
Ran for 62550 frames Cell Events: 3389 mean_circ: 0.939414
Ran for 63550 frames Cell Events: 3459 mean_circ: 0.939404
Ran for 66050 frames Cell Events: 3590 mean_circ: 0.939373
Ran for 66550 frames Cell Events: 3619 mean_circ: 0.939339
Ran for 68050 frames Cell Events: 3707 mean_circ: 0.9394
Ran for 68550 frames Cell Events: 3729 mean_circ: 0.939382
Ran for 69550 frames Cell Events: 3768 mean_circ: 0.939403
Ran for 70050 frames Cell Events: 3799 mean_circ: 0.939387
Ran for 70550 frames Cell Events: 3815 mean_circ: 0.939457
Ran for 71050 frames Cell Events: 3838 mean_circ: 0.939408
Ran for 72050 frames Cell Events: 3898 mean_circ: 0.939221
Ran for 72550 frames Cell Events: 3924 mean_circ: 0.939232
Ran for 73050 frames Cell Events: 3952 mean_circ: 0.939225
Ran for 73550 frames Cell Events: 3974 mean_circ: 0.939164
Ran for 74550 frames Cell Events: 4028 mean_circ: 0.939088
Ran for 76050 frames Cell Events: 4119 mean_circ: 0.939211
Ran for 78050 frames Cell Events: 4223 mean_circ: 0.93924
Ran for 78550 frames Cell Events: 4250 mean_circ: 0.939276
Ran for 80550 frames Cell Events: 4360 mean_circ: 0.939101
Ran for 83550 frames Cell Events: 4523 mean_circ: 0.93914
Ran for 84550 frames Cell Events: 4582 mean_circ: 0.939123
Ran for 85050 frames Cell Events: 4608 mean_circ: 0.939156
Ran for 85550 frames Cell Events: 4633 mean_circ: 0.939179
Ran for 87050 frames Cell Events: 4710 mean_circ: 0.939216
Ran for 88050 frames Cell Events: 4766 mean_circ: 0.939203
Ran for 89050 frames Cell Events: 4827 mean_circ: 0.939102
Ran for 91050 frames Cell Events: 4956 mean_circ: 0.939183
Ran for 91550 frames Cell Events: 4986 mean_circ: 0.939228
Ran for 92550 frames Cell Events: 5039 mean_circ: 0.93918
Ran for 93050 frames Cell Events: 5061 mean_circ: 0.939182
Ran for 93550 frames Cell Events: 5083 mean_circ: 0.939162
Ran for 95550 frames Cell Events: 5182 mean_circ: 0.939155
Ran for 96050 frames Cell Events: 5208 mean_circ: 0.939117
Ran for 96550 frames Cell Events: 5241 mean_circ: 0.939128
Ran for 97050 frames Cell Events: 5263 mean_circ: 0.939099
Ran for 97550 frames Cell Events: 5286 mean_circ: 0.9391
Ran for 98050 frames Cell Events: 5310 mean_circ: 0.9391
Ran for 98550 frames Cell Events: 5335 mean_circ: 0.939049
Ran for 100050 frames Cell Events: 5423 mean_circ: 0.939217
Ran for 101050 frames Cell Events: 5472 mean_circ: 0.939318
Ran for 102050 frames Cell Events: 5536 mean_circ: 0.93935
Ran for 103050 frames Cell Events: 5590 mean_circ: 0.9394
Ran for 103550 frames Cell Events: 5612 mean_circ: 0.939345
Ran for 104050 frames Cell Events: 5642 mean_circ: 0.939324
Ran for 104550 frames Cell Events: 5677 mean_circ: 0.939316
Ran for 105550 frames Cell Events: 5741 mean_circ: 0.939351
Ran for 107550 frames Cell Events: 5850 mean_circ: 0.93945
Ran for 109050 frames Cell Events: 5934 mean_circ: 0.939379
Ran for 111550 frames Cell Events: 6097 mean_circ: 0.939254
Ran for 112550 frames Cell Events: 6147 mean_circ: 0.939238
Ran for 113550 frames Cell Events: 6214 mean_circ: 0.939313
Ran for 114050 frames Cell Events: 6241 mean_circ: 0.939301
Ran for 115050 frames Cell Events: 6304 mean_circ: 0.939307
Ran for 115550 frames Cell Events: 6319 mean_circ: 0.939317
Ran for 116550 frames Cell Events: 6375 mean_circ: 0.939385
Ran for 117050 frames Cell Events: 6396 mean_circ: 0.93939
Ran for 120550 frames Cell Events: 6639 mean_circ: 0.939213
Ran for 121550 frames Cell Events: 6701 mean_circ: 0.939228
Ran for 122550 frames Cell Events: 6762 mean_circ: 0.939298
Ran for 123550 frames Cell Events: 6809 mean_circ: 0.939313
Ran for 124050 frames Cell Events: 6846 mean_circ: 0.939345
Ran for 125050 frames Cell Events: 6902 mean_circ: 0.93935
Ran for 126550 frames Cell Events: 6990 mean_circ: 0.939405
Ran for 129050 frames Cell Events: 7138 mean_circ: 0.939334
Ran for 130550 frames Cell Events: 7242 mean_circ: 0.939303
Ran for 132050 frames Cell Events: 7320 mean_circ: 0.939289
Ran for 132550 frames Cell Events: 7367 mean_circ: 0.939289
Ran for 133050 frames Cell Events: 7401 mean_circ: 0.939289
Ran for 133550 frames Cell Events: 7431 mean_circ: 0.939237
Ran for 134550 frames Cell Events: 7495 mean_circ: 0.939235
Ran for 135050 frames Cell Events: 7516 mean_circ: 0.939239
Ran for 136050 frames Cell Events: 7564 mean_circ: 0.939237
Ran for 136550 frames Cell Events: 7587 mean_circ: 0.93923
Ran for 138050 frames Cell Events: 7672 mean_circ: 0.939171
Ran for 139050 frames Cell Events: 7749 mean_circ: 0.939152
Ran for 139550 frames Cell Events: 7782 mean_circ: 0.939186
Ran for 140550 frames Cell Events: 7837 mean_circ: 0.939177
Ran for 141550 frames Cell Events: 7887 mean_circ: 0.939214
Ran for 142550 frames Cell Events: 7947 mean_circ: 0.939222
Ran for 144050 frames Cell Events: 8015 mean_circ: 0.939195
Ran for 147050 frames Cell Events: 8205 mean_circ: 0.939138
Ran for 148050 frames Cell Events: 8280 mean_circ: 0.939123
Ran for 149050 frames Cell Events: 8341 mean_circ: 0.939062
Ran for 149550 frames Cell Events: 8364 mean_circ: 0.939047
Ran for 150050 frames Cell Events: 8383 mean_circ: 0.939045
Ran for 152050 frames Cell Events: 8497 mean_circ: 0.939036
Ran for 152550 frames Cell Events: 8526 mean_circ: 0.939018
Ran for 153050 frames Cell Events: 8561 mean_circ: 0.939014
Ran for 155050 frames Cell Events: 8658 mean_circ: 0.938996
Ran for 155550 frames Cell Events: 8690 mean_circ: 0.939019
Ran for 157050 frames Cell Events: 8785 mean_circ: 0.939041
Ran for 157550 frames Cell Events: 8814 mean_circ: 0.939056
Ran for 159550 frames Cell Events: 8960 mean_circ: 0.93906
Ran for 160050 frames Cell Events: 8991 mean_circ: 0.939023



End this measurment..



Stopping time: 2.53076e+009
****************************************
Measurement finished

Mean Circularitiy: 0.938962
Mean Area: 596.624
Mean FL-1 intensity: 2991.94
Mean FL-2 intensity: 3531.83
Mean FL-3 intensity: 0

frames processed: 160750
Total Time: 81124.2ms
Number of Runs (new bg img) 0
Frames collected: 162129
Skipped frames: 19154
Rescued Frames: 2200885
Samples DAQ: 40532285
Samples written: 81064518
Samples per Channel: 40532259
FPS: 1998.53

Samples per second: 499632
Expected samples/s: 500000

Events: 9147
Events per second: 112.753
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :251475
# 1 frames :57848
# 2 frames :3728
# 3 frames :1178
# 4 frames :810
# 5 frames :348
# 6 frames :1908
# 7 frames :1438
# 8 frames :1428
# 9 frames :831
# 10 frames :579
# 11 frames :344
# 12 frames :317
# 13 frames :187
# 14 frames :247
# 15 frames :217
# 16 frames :200
# 17 frames :140
# 18 frames :116
# 19 frames :81
# 20 frames :55
# 21 frames :72
# 22 frames :63
# 23 frames :44
# 24 frames :41
# 25 frames :33
# 26 frames :34
# 27 frames :33
# 28 frames :18
# 29 frames :26
# 30 frames :19
# 31 frames :14
# 32 frames :10
# 33 frames :13
# 34 frames :18
# 35 frames :6
# 36 frames :11
# 37 frames :7
# 38 frames :3
# 39 frames :6
# 40 frames :9
# 41 frames :4
# 42 frames :3
# 43 frames :2
# 45 frames :1
# 46 frames :2
# 47 frames :1
# 48 frames :7
# 50 frames :2
# 52 frames :2
# 54 frames :1
# 55 frames :3
# 57 frames :2
# 63 frames :3
# 70 frames :1
# 72 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7149
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0