Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.21.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5cf6cab95eb7d88c19dfd852e788a34e",
"name": "M1_20170613_1705_dsRNA_Y4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:21:46"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8242 (max 8243) in '8242'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6095
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53695e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 89 mean_circ: 0.919444
Ran for 2050 frames Cell Events: 109 mean_circ: 0.915347
Ran for 3550 frames Cell Events: 189 mean_circ: 0.914924
Ran for 4550 frames Cell Events: 234 mean_circ: 0.916153
Ran for 5550 frames Cell Events: 278 mean_circ: 0.916406
Ran for 10050 frames Cell Events: 523 mean_circ: 0.917074
Ran for 10550 frames Cell Events: 542 mean_circ: 0.916839
Ran for 11550 frames Cell Events: 578 mean_circ: 0.917371
Ran for 15050 frames Cell Events: 768 mean_circ: 0.916925
Ran for 16050 frames Cell Events: 823 mean_circ: 0.917144
Ran for 16550 frames Cell Events: 848 mean_circ: 0.916806
Ran for 18050 frames Cell Events: 914 mean_circ: 0.91677
Ran for 18550 frames Cell Events: 933 mean_circ: 0.9166
Ran for 19050 frames Cell Events: 965 mean_circ: 0.916949
Ran for 20050 frames Cell Events: 1016 mean_circ: 0.917266
Ran for 21050 frames Cell Events: 1063 mean_circ: 0.917583
Ran for 23050 frames Cell Events: 1143 mean_circ: 0.917869
Ran for 24050 frames Cell Events: 1199 mean_circ: 0.918002
Ran for 24550 frames Cell Events: 1225 mean_circ: 0.918302
Ran for 26050 frames Cell Events: 1318 mean_circ: 0.918302
Ran for 26550 frames Cell Events: 1336 mean_circ: 0.918262
Ran for 29550 frames Cell Events: 1478 mean_circ: 0.918594
Ran for 30050 frames Cell Events: 1505 mean_circ: 0.918346
Ran for 30550 frames Cell Events: 1533 mean_circ: 0.918415
Ran for 31550 frames Cell Events: 1575 mean_circ: 0.918358
Ran for 32050 frames Cell Events: 1593 mean_circ: 0.918428
Ran for 34550 frames Cell Events: 1743 mean_circ: 0.919004
Ran for 35050 frames Cell Events: 1781 mean_circ: 0.919092
Ran for 35550 frames Cell Events: 1809 mean_circ: 0.918943
Ran for 37050 frames Cell Events: 1881 mean_circ: 0.918762
Ran for 37550 frames Cell Events: 1903 mean_circ: 0.918695
Ran for 38550 frames Cell Events: 1957 mean_circ: 0.918685
Ran for 39550 frames Cell Events: 1997 mean_circ: 0.918426
Ran for 40050 frames Cell Events: 2016 mean_circ: 0.918408
Ran for 40550 frames Cell Events: 2038 mean_circ: 0.918308
Ran for 41050 frames Cell Events: 2075 mean_circ: 0.918462
Ran for 42050 frames Cell Events: 2102 mean_circ: 0.918636
Ran for 43050 frames Cell Events: 2164 mean_circ: 0.918778
Ran for 44050 frames Cell Events: 2226 mean_circ: 0.918446
Ran for 44550 frames Cell Events: 2247 mean_circ: 0.918602
Ran for 45050 frames Cell Events: 2275 mean_circ: 0.918637
Ran for 45550 frames Cell Events: 2304 mean_circ: 0.918681
Ran for 46550 frames Cell Events: 2346 mean_circ: 0.918639
Ran for 48550 frames Cell Events: 2446 mean_circ: 0.918611
Ran for 49550 frames Cell Events: 2497 mean_circ: 0.918674
Ran for 50050 frames Cell Events: 2527 mean_circ: 0.918618
Ran for 51050 frames Cell Events: 2570 mean_circ: 0.918541
Ran for 52050 frames Cell Events: 2613 mean_circ: 0.918665
Ran for 52550 frames Cell Events: 2629 mean_circ: 0.918649
Ran for 53550 frames Cell Events: 2679 mean_circ: 0.918597
Ran for 54550 frames Cell Events: 2751 mean_circ: 0.918296
Ran for 55550 frames Cell Events: 2798 mean_circ: 0.918262
Ran for 56050 frames Cell Events: 2822 mean_circ: 0.918235
Ran for 56550 frames Cell Events: 2844 mean_circ: 0.918279
Ran for 57050 frames Cell Events: 2868 mean_circ: 0.918344
Ran for 57550 frames Cell Events: 2893 mean_circ: 0.918263
Ran for 58050 frames Cell Events: 2908 mean_circ: 0.918209
Ran for 59050 frames Cell Events: 2959 mean_circ: 0.918199
Ran for 59550 frames Cell Events: 2978 mean_circ: 0.918032
Ran for 60550 frames Cell Events: 3012 mean_circ: 0.91804
Ran for 61550 frames Cell Events: 3046 mean_circ: 0.918173
Ran for 62050 frames Cell Events: 3066 mean_circ: 0.918235
Ran for 63550 frames Cell Events: 3147 mean_circ: 0.918366
Ran for 64050 frames Cell Events: 3168 mean_circ: 0.918249
Ran for 64550 frames Cell Events: 3185 mean_circ: 0.918272
Ran for 65050 frames Cell Events: 3213 mean_circ: 0.918332
Ran for 66050 frames Cell Events: 3254 mean_circ: 0.918556
Ran for 66550 frames Cell Events: 3277 mean_circ: 0.918546
Ran for 67050 frames Cell Events: 3305 mean_circ: 0.918446
Ran for 67550 frames Cell Events: 3328 mean_circ: 0.91845
Ran for 68550 frames Cell Events: 3371 mean_circ: 0.918317
Ran for 69550 frames Cell Events: 3411 mean_circ: 0.918422
Ran for 70050 frames Cell Events: 3434 mean_circ: 0.918337
Ran for 70550 frames Cell Events: 3454 mean_circ: 0.918401
Ran for 71550 frames Cell Events: 3500 mean_circ: 0.91842
Ran for 74550 frames Cell Events: 3658 mean_circ: 0.91819
Ran for 75550 frames Cell Events: 3694 mean_circ: 0.918163
Ran for 76050 frames Cell Events: 3723 mean_circ: 0.918163
Ran for 76550 frames Cell Events: 3755 mean_circ: 0.918054
Ran for 77050 frames Cell Events: 3775 mean_circ: 0.918118
Ran for 78050 frames Cell Events: 3800 mean_circ: 0.918191
Ran for 79050 frames Cell Events: 3838 mean_circ: 0.91833
Ran for 80050 frames Cell Events: 3892 mean_circ: 0.918284
Ran for 80550 frames Cell Events: 3917 mean_circ: 0.918257
Ran for 81050 frames Cell Events: 3943 mean_circ: 0.918244
Ran for 81550 frames Cell Events: 3961 mean_circ: 0.918165
Ran for 83050 frames Cell Events: 4050 mean_circ: 0.918082
Ran for 85050 frames Cell Events: 4151 mean_circ: 0.918175
Ran for 86050 frames Cell Events: 4206 mean_circ: 0.918264
Ran for 86550 frames Cell Events: 4232 mean_circ: 0.918335
Ran for 87050 frames Cell Events: 4261 mean_circ: 0.918248
Ran for 88550 frames Cell Events: 4328 mean_circ: 0.918206
Ran for 90050 frames Cell Events: 4406 mean_circ: 0.918221
Ran for 91550 frames Cell Events: 4468 mean_circ: 0.918375
Ran for 92050 frames Cell Events: 4488 mean_circ: 0.918432
Ran for 93550 frames Cell Events: 4554 mean_circ: 0.918291
Ran for 94550 frames Cell Events: 4604 mean_circ: 0.91836
Ran for 95050 frames Cell Events: 4621 mean_circ: 0.918399
Ran for 95550 frames Cell Events: 4638 mean_circ: 0.918404
Ran for 96050 frames Cell Events: 4660 mean_circ: 0.918395
Ran for 98050 frames Cell Events: 4746 mean_circ: 0.918384
Ran for 99050 frames Cell Events: 4785 mean_circ: 0.918284
Ran for 99550 frames Cell Events: 4812 mean_circ: 0.918272
Ran for 100550 frames Cell Events: 4865 mean_circ: 0.918378
Ran for 101050 frames Cell Events: 4885 mean_circ: 0.918352
Ran for 101550 frames Cell Events: 4921 mean_circ: 0.918346
Ran for 102050 frames Cell Events: 4962 mean_circ: 0.918417
Ran for 102550 frames Cell Events: 4989 mean_circ: 0.918336
Ran for 104050 frames Cell Events: 5062 mean_circ: 0.918311
Ran for 104550 frames Cell Events: 5083 mean_circ: 0.918336
Ran for 105050 frames Cell Events: 5104 mean_circ: 0.91837
Ran for 106550 frames Cell Events: 5186 mean_circ: 0.918387
Ran for 107050 frames Cell Events: 5218 mean_circ: 0.918384
Ran for 108050 frames Cell Events: 5276 mean_circ: 0.918389
Ran for 109050 frames Cell Events: 5331 mean_circ: 0.918441
Ran for 111050 frames Cell Events: 5443 mean_circ: 0.918513
Ran for 111550 frames Cell Events: 5462 mean_circ: 0.918454
Ran for 112050 frames Cell Events: 5477 mean_circ: 0.918511
Ran for 113050 frames Cell Events: 5509 mean_circ: 0.918592
Ran for 114050 frames Cell Events: 5569 mean_circ: 0.91862
Ran for 115050 frames Cell Events: 5642 mean_circ: 0.918605
Ran for 117050 frames Cell Events: 5736 mean_circ: 0.918632
Ran for 119050 frames Cell Events: 5850 mean_circ: 0.918616
Ran for 119550 frames Cell Events: 5872 mean_circ: 0.918632
Ran for 120050 frames Cell Events: 5888 mean_circ: 0.918589
Ran for 120550 frames Cell Events: 5910 mean_circ: 0.918636
Ran for 121550 frames Cell Events: 5943 mean_circ: 0.918617
Ran for 122550 frames Cell Events: 5985 mean_circ: 0.918629
Ran for 123050 frames Cell Events: 6006 mean_circ: 0.918667
Ran for 123550 frames Cell Events: 6034 mean_circ: 0.918603
Ran for 124550 frames Cell Events: 6070 mean_circ: 0.91861
Ran for 125050 frames Cell Events: 6092 mean_circ: 0.918642
Ran for 126550 frames Cell Events: 6183 mean_circ: 0.918598
Ran for 128050 frames Cell Events: 6258 mean_circ: 0.918652
Ran for 128550 frames Cell Events: 6274 mean_circ: 0.918725
Ran for 130050 frames Cell Events: 6349 mean_circ: 0.918687
Ran for 131050 frames Cell Events: 6394 mean_circ: 0.918662
Ran for 131550 frames Cell Events: 6411 mean_circ: 0.918689
Ran for 132050 frames Cell Events: 6442 mean_circ: 0.918728
Ran for 133550 frames Cell Events: 6537 mean_circ: 0.918714
Ran for 135050 frames Cell Events: 6617 mean_circ: 0.918706
Ran for 136050 frames Cell Events: 6653 mean_circ: 0.918704
Ran for 138050 frames Cell Events: 6723 mean_circ: 0.918774
Ran for 138550 frames Cell Events: 6750 mean_circ: 0.918774
Ran for 141050 frames Cell Events: 6877 mean_circ: 0.918766
Ran for 142050 frames Cell Events: 6923 mean_circ: 0.918922
Ran for 142550 frames Cell Events: 6952 mean_circ: 0.918947
Ran for 144550 frames Cell Events: 7057 mean_circ: 0.919009
Ran for 145550 frames Cell Events: 7118 mean_circ: 0.919026
Ran for 146050 frames Cell Events: 7150 mean_circ: 0.919033
Ran for 146550 frames Cell Events: 7173 mean_circ: 0.919069
Ran for 147050 frames Cell Events: 7194 mean_circ: 0.919078
Ran for 149550 frames Cell Events: 7317 mean_circ: 0.919118
Ran for 150050 frames Cell Events: 7339 mean_circ: 0.919144
Ran for 150550 frames Cell Events: 7357 mean_circ: 0.919192
Ran for 153050 frames Cell Events: 7462 mean_circ: 0.919175
Ran for 155050 frames Cell Events: 7556 mean_circ: 0.91915
Ran for 157050 frames Cell Events: 7669 mean_circ: 0.919206
Ran for 158050 frames Cell Events: 7739 mean_circ: 0.919213
Ran for 158550 frames Cell Events: 7763 mean_circ: 0.919205
Ran for 159550 frames Cell Events: 7818 mean_circ: 0.919163
Ran for 160050 frames Cell Events: 7844 mean_circ: 0.919215
Ran for 161050 frames Cell Events: 7913 mean_circ: 0.91926
Ran for 162050 frames Cell Events: 7957 mean_circ: 0.919241
Ran for 162550 frames Cell Events: 7983 mean_circ: 0.919195
Ran for 163050 frames Cell Events: 8016 mean_circ: 0.919187
Ran for 163550 frames Cell Events: 8042 mean_circ: 0.919147
Ran for 164050 frames Cell Events: 8076 mean_circ: 0.919158
Ran for 165050 frames Cell Events: 8110 mean_circ: 0.919163
Ran for 165550 frames Cell Events: 8143 mean_circ: 0.919173
Ran for 166050 frames Cell Events: 8162 mean_circ: 0.919181
Ran for 167050 frames Cell Events: 8210 mean_circ: 0.919208



End this measurment..



Stopping time: 2.53704e+009
****************************************
Measurement finished

Mean Circularitiy: 0.919203
Mean Area: 744.576
Mean FL-1 intensity: 3076.76
Mean FL-2 intensity: 3779.99
Mean FL-3 intensity: 0

frames processed: 166025
Total Time: 83902.4ms
Number of Runs (new bg img) 0
Frames collected: 167642
Skipped frames: 35325
Rescued Frames: 3830138
Samples DAQ: 41910506
Samples written: 83820930
Samples per Channel: 41910465
FPS: 1998.06

Samples per second: 499514
Expected samples/s: 500000

Events: 8243
Events per second: 98.2451
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :261021
# 1 frames :60353
# 2 frames :3308
# 3 frames :1242
# 4 frames :779
# 5 frames :390
# 6 frames :1161
# 7 frames :1310
# 8 frames :1095
# 9 frames :745
# 10 frames :651
# 11 frames :477
# 12 frames :394
# 13 frames :276
# 14 frames :232
# 15 frames :233
# 16 frames :182
# 17 frames :174
# 18 frames :147
# 19 frames :133
# 20 frames :97
# 21 frames :91
# 22 frames :76
# 23 frames :50
# 24 frames :61
# 25 frames :41
# 26 frames :31
# 27 frames :44
# 28 frames :43
# 29 frames :21
# 30 frames :28
# 31 frames :25
# 32 frames :16
# 33 frames :20
# 34 frames :18
# 35 frames :14
# 36 frames :11
# 37 frames :15
# 38 frames :9
# 39 frames :11
# 40 frames :7
# 41 frames :8
# 42 frames :5
# 43 frames :3
# 44 frames :4
# 45 frames :2
# 46 frames :4
# 47 frames :3
# 48 frames :4
# 49 frames :6
# 50 frames :4
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :4
# 55 frames :3
# 56 frames :4
# 57 frames :1
# 58 frames :1
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 66 frames :1
# 67 frames :1
# 73 frames :1
# 74 frames :1
# 78 frames :1
# 84 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6095
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0