Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.24.39 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7e486c8a403a2d6745597a69cbcd7985",
"name": "M1_20170613_1754_dsRNA_V3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:25:01"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8680 (max 8681) in '8680'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7948
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.53987e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 150 mean_circ: 0.942638
Ran for 4050 frames Cell Events: 268 mean_circ: 0.945131
Ran for 5050 frames Cell Events: 318 mean_circ: 0.947076
Ran for 6550 frames Cell Events: 404 mean_circ: 0.947636
Ran for 7550 frames Cell Events: 483 mean_circ: 0.94801
Ran for 8550 frames Cell Events: 527 mean_circ: 0.948576
Ran for 10050 frames Cell Events: 645 mean_circ: 0.948093
Ran for 10550 frames Cell Events: 671 mean_circ: 0.948329
Ran for 11050 frames Cell Events: 706 mean_circ: 0.948364
Ran for 12050 frames Cell Events: 752 mean_circ: 0.948097
Ran for 12550 frames Cell Events: 775 mean_circ: 0.947749
Ran for 13550 frames Cell Events: 822 mean_circ: 0.947918
Ran for 15050 frames Cell Events: 912 mean_circ: 0.948088
Ran for 15550 frames Cell Events: 948 mean_circ: 0.948128
Ran for 16550 frames Cell Events: 1013 mean_circ: 0.948055
Ran for 17050 frames Cell Events: 1037 mean_circ: 0.948114
Ran for 17550 frames Cell Events: 1076 mean_circ: 0.947926
Ran for 20050 frames Cell Events: 1267 mean_circ: 0.948629
Ran for 21050 frames Cell Events: 1318 mean_circ: 0.948914
Ran for 21550 frames Cell Events: 1350 mean_circ: 0.949035
Ran for 22550 frames Cell Events: 1388 mean_circ: 0.948939
Ran for 24050 frames Cell Events: 1477 mean_circ: 0.949011
Ran for 24550 frames Cell Events: 1508 mean_circ: 0.948934
Ran for 27550 frames Cell Events: 1729 mean_circ: 0.948723
Ran for 28050 frames Cell Events: 1765 mean_circ: 0.948723
Ran for 29050 frames Cell Events: 1825 mean_circ: 0.948792
Ran for 30550 frames Cell Events: 1912 mean_circ: 0.948895
Ran for 31050 frames Cell Events: 1948 mean_circ: 0.948968
Ran for 31550 frames Cell Events: 1984 mean_circ: 0.948968
Ran for 32050 frames Cell Events: 2009 mean_circ: 0.948905
Ran for 32550 frames Cell Events: 2031 mean_circ: 0.948859
Ran for 33550 frames Cell Events: 2095 mean_circ: 0.949165
Ran for 34550 frames Cell Events: 2158 mean_circ: 0.949392
Ran for 36050 frames Cell Events: 2248 mean_circ: 0.949429
Ran for 37550 frames Cell Events: 2342 mean_circ: 0.9495
Ran for 38550 frames Cell Events: 2406 mean_circ: 0.949373
Ran for 39050 frames Cell Events: 2426 mean_circ: 0.949333
Ran for 42050 frames Cell Events: 2618 mean_circ: 0.949431
Ran for 43550 frames Cell Events: 2717 mean_circ: 0.949254
Ran for 44550 frames Cell Events: 2782 mean_circ: 0.949293
Ran for 45050 frames Cell Events: 2810 mean_circ: 0.949367
Ran for 47550 frames Cell Events: 2992 mean_circ: 0.949329
Ran for 48050 frames Cell Events: 3021 mean_circ: 0.949304
Ran for 48550 frames Cell Events: 3048 mean_circ: 0.949257
Ran for 49050 frames Cell Events: 3082 mean_circ: 0.949324
Ran for 50050 frames Cell Events: 3143 mean_circ: 0.949304
Ran for 51050 frames Cell Events: 3204 mean_circ: 0.949266
Ran for 51550 frames Cell Events: 3248 mean_circ: 0.949266
Ran for 52050 frames Cell Events: 3274 mean_circ: 0.949268
Ran for 53050 frames Cell Events: 3321 mean_circ: 0.949104
Ran for 56550 frames Cell Events: 3534 mean_circ: 0.949014
Ran for 58050 frames Cell Events: 3641 mean_circ: 0.948973
Ran for 58550 frames Cell Events: 3686 mean_circ: 0.9489
Ran for 59550 frames Cell Events: 3752 mean_circ: 0.948782
Ran for 60550 frames Cell Events: 3814 mean_circ: 0.948831
Ran for 61050 frames Cell Events: 3842 mean_circ: 0.948845
Ran for 61550 frames Cell Events: 3874 mean_circ: 0.948818
Ran for 62550 frames Cell Events: 3948 mean_circ: 0.948818
Ran for 63550 frames Cell Events: 4006 mean_circ: 0.948877
Ran for 65050 frames Cell Events: 4095 mean_circ: 0.94908
Ran for 65550 frames Cell Events: 4120 mean_circ: 0.949091
Ran for 67550 frames Cell Events: 4234 mean_circ: 0.949185
Ran for 69550 frames Cell Events: 4354 mean_circ: 0.949243
Ran for 70550 frames Cell Events: 4421 mean_circ: 0.949219
Ran for 71050 frames Cell Events: 4439 mean_circ: 0.949154
Ran for 71550 frames Cell Events: 4463 mean_circ: 0.949184
Ran for 72050 frames Cell Events: 4487 mean_circ: 0.949132
Ran for 73550 frames Cell Events: 4588 mean_circ: 0.949077
Ran for 76550 frames Cell Events: 4784 mean_circ: 0.948944
Ran for 77050 frames Cell Events: 4809 mean_circ: 0.948992
Ran for 77550 frames Cell Events: 4844 mean_circ: 0.949003
Ran for 78050 frames Cell Events: 4880 mean_circ: 0.949066
Ran for 81550 frames Cell Events: 5100 mean_circ: 0.949098
Ran for 82050 frames Cell Events: 5135 mean_circ: 0.949108
Ran for 82550 frames Cell Events: 5160 mean_circ: 0.948966
Ran for 83050 frames Cell Events: 5183 mean_circ: 0.948951
Ran for 84050 frames Cell Events: 5276 mean_circ: 0.948951
Ran for 84550 frames Cell Events: 5313 mean_circ: 0.94903
Ran for 85550 frames Cell Events: 5372 mean_circ: 0.949023
Ran for 87050 frames Cell Events: 5472 mean_circ: 0.949091
Ran for 88050 frames Cell Events: 5515 mean_circ: 0.949103
Ran for 92550 frames Cell Events: 5860 mean_circ: 0.948635
Ran for 93050 frames Cell Events: 5894 mean_circ: 0.948512
Ran for 94050 frames Cell Events: 5948 mean_circ: 0.948462
Ran for 94550 frames Cell Events: 5987 mean_circ: 0.948445
Ran for 95550 frames Cell Events: 6053 mean_circ: 0.948422
Ran for 96050 frames Cell Events: 6088 mean_circ: 0.948406
Ran for 96550 frames Cell Events: 6122 mean_circ: 0.948404
Ran for 97550 frames Cell Events: 6184 mean_circ: 0.94841
Ran for 98550 frames Cell Events: 6237 mean_circ: 0.948349
Ran for 100050 frames Cell Events: 6341 mean_circ: 0.948292
Ran for 100550 frames Cell Events: 6363 mean_circ: 0.948291
Ran for 102050 frames Cell Events: 6462 mean_circ: 0.948326
Ran for 102550 frames Cell Events: 6481 mean_circ: 0.948312
Ran for 103550 frames Cell Events: 6540 mean_circ: 0.948336
Ran for 104050 frames Cell Events: 6563 mean_circ: 0.948392
Ran for 105050 frames Cell Events: 6623 mean_circ: 0.948395
Ran for 105550 frames Cell Events: 6657 mean_circ: 0.948377
Ran for 108050 frames Cell Events: 6840 mean_circ: 0.948348
Ran for 110050 frames Cell Events: 6984 mean_circ: 0.948342
Ran for 110550 frames Cell Events: 7022 mean_circ: 0.948337
Ran for 111050 frames Cell Events: 7053 mean_circ: 0.94834
Ran for 112550 frames Cell Events: 7167 mean_circ: 0.948324
Ran for 115050 frames Cell Events: 7344 mean_circ: 0.948277
Ran for 116550 frames Cell Events: 7467 mean_circ: 0.9483
Ran for 117050 frames Cell Events: 7512 mean_circ: 0.948307
Ran for 117550 frames Cell Events: 7549 mean_circ: 0.948307
Ran for 118050 frames Cell Events: 7585 mean_circ: 0.948302
Ran for 121050 frames Cell Events: 7811 mean_circ: 0.948329
Ran for 121550 frames Cell Events: 7849 mean_circ: 0.948338
Ran for 122050 frames Cell Events: 7887 mean_circ: 0.948321
Ran for 123550 frames Cell Events: 8006 mean_circ: 0.948354
Ran for 124050 frames Cell Events: 8032 mean_circ: 0.948392
Ran for 124550 frames Cell Events: 8057 mean_circ: 0.948374
Ran for 128050 frames Cell Events: 8330 mean_circ: 0.948435
Ran for 130050 frames Cell Events: 8473 mean_circ: 0.948412
Ran for 130550 frames Cell Events: 8508 mean_circ: 0.948424



End this measurment..



Stopping time: 2.53994e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948518
Mean Area: 586.813
Mean FL-1 intensity: 3326.96
Mean FL-2 intensity: 3038.78
Mean FL-3 intensity: 0

frames processed: 128989
Total Time: 66568.4ms
Number of Runs (new bg img) 0
Frames collected: 132967
Skipped frames: 48508
Rescued Frames: 4520301
Samples DAQ: 33242100
Samples written: 66483522
Samples per Channel: 33241761
FPS: 1997.45

Samples per second: 499363
Expected samples/s: 500000

Events: 8681
Events per second: 130.407
jumped back (PMT): 3201
#Frames read, frequency
# 0 frames :217424
# 1 frames :35434
# 2 frames :2540
# 3 frames :886
# 4 frames :574
# 5 frames :324
# 6 frames :1271
# 7 frames :996
# 8 frames :934
# 9 frames :576
# 10 frames :476
# 11 frames :307
# 12 frames :243
# 13 frames :187
# 14 frames :228
# 15 frames :209
# 16 frames :181
# 17 frames :150
# 18 frames :120
# 19 frames :92
# 20 frames :89
# 21 frames :94
# 22 frames :66
# 23 frames :70
# 24 frames :59
# 25 frames :44
# 26 frames :38
# 27 frames :37
# 28 frames :48
# 29 frames :42
# 30 frames :34
# 31 frames :33
# 32 frames :27
# 33 frames :24
# 34 frames :17
# 35 frames :16
# 36 frames :22
# 37 frames :17
# 38 frames :23
# 39 frames :15
# 40 frames :10
# 41 frames :13
# 42 frames :5
# 43 frames :8
# 44 frames :9
# 45 frames :5
# 46 frames :5
# 47 frames :7
# 48 frames :11
# 49 frames :11
# 50 frames :3
# 51 frames :6
# 52 frames :2
# 53 frames :4
# 54 frames :3
# 55 frames :5
# 56 frames :3
# 57 frames :3
# 58 frames :2
# 59 frames :3
# 60 frames :1
# 61 frames :2
# 62 frames :4
# 63 frames :4
# 64 frames :1
# 66 frames :1
# 67 frames :1
# 68 frames :2
# 69 frames :3
# 70 frames :1
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# 88 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7948
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0