Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.25.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0a6559dd25a6381d3332077c37e6da63",
"name": "M1_20170613_1805_dsRNA_X7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:25:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8260 (max 8261) in '8260'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7807
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54051e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 50 mean_circ: 0.944528
Ran for 1550 frames Cell Events: 94 mean_circ: 0.944999
Ran for 2550 frames Cell Events: 160 mean_circ: 0.947039
Ran for 3050 frames Cell Events: 196 mean_circ: 0.945194
Ran for 4050 frames Cell Events: 258 mean_circ: 0.945174
Ran for 6550 frames Cell Events: 433 mean_circ: 0.947023
Ran for 7050 frames Cell Events: 479 mean_circ: 0.947023
Ran for 7550 frames Cell Events: 510 mean_circ: 0.94753
Ran for 8050 frames Cell Events: 535 mean_circ: 0.947593
Ran for 8550 frames Cell Events: 572 mean_circ: 0.948302
Ran for 9050 frames Cell Events: 609 mean_circ: 0.94859
Ran for 11050 frames Cell Events: 719 mean_circ: 0.948487
Ran for 11550 frames Cell Events: 739 mean_circ: 0.948343
Ran for 12050 frames Cell Events: 767 mean_circ: 0.94841
Ran for 13550 frames Cell Events: 846 mean_circ: 0.94789
Ran for 14050 frames Cell Events: 884 mean_circ: 0.947757
Ran for 15550 frames Cell Events: 965 mean_circ: 0.948729
Ran for 16050 frames Cell Events: 999 mean_circ: 0.948492
Ran for 16550 frames Cell Events: 1037 mean_circ: 0.948224
Ran for 17050 frames Cell Events: 1063 mean_circ: 0.94809
Ran for 18550 frames Cell Events: 1181 mean_circ: 0.947822
Ran for 19050 frames Cell Events: 1211 mean_circ: 0.947884
Ran for 19550 frames Cell Events: 1251 mean_circ: 0.947849
Ran for 20050 frames Cell Events: 1271 mean_circ: 0.947618
Ran for 21050 frames Cell Events: 1355 mean_circ: 0.947754
Ran for 22050 frames Cell Events: 1425 mean_circ: 0.947723
Ran for 24050 frames Cell Events: 1538 mean_circ: 0.947247
Ran for 24550 frames Cell Events: 1573 mean_circ: 0.947342
Ran for 26550 frames Cell Events: 1724 mean_circ: 0.947149
Ran for 27050 frames Cell Events: 1748 mean_circ: 0.94727
Ran for 28050 frames Cell Events: 1817 mean_circ: 0.947455
Ran for 30050 frames Cell Events: 1972 mean_circ: 0.947183
Ran for 34050 frames Cell Events: 2245 mean_circ: 0.947193
Ran for 35550 frames Cell Events: 2353 mean_circ: 0.947351
Ran for 36050 frames Cell Events: 2390 mean_circ: 0.947314
Ran for 37050 frames Cell Events: 2448 mean_circ: 0.947179
Ran for 37550 frames Cell Events: 2475 mean_circ: 0.947323
Ran for 40050 frames Cell Events: 2682 mean_circ: 0.947423
Ran for 43050 frames Cell Events: 2892 mean_circ: 0.947362
Ran for 49550 frames Cell Events: 3327 mean_circ: 0.947179
Ran for 51550 frames Cell Events: 3424 mean_circ: 0.947074
Ran for 56050 frames Cell Events: 3784 mean_circ: 0.946797
Ran for 57050 frames Cell Events: 3864 mean_circ: 0.946748
Ran for 57550 frames Cell Events: 3893 mean_circ: 0.94679
Ran for 59050 frames Cell Events: 4000 mean_circ: 0.946828
Ran for 60550 frames Cell Events: 4115 mean_circ: 0.946875
Ran for 61550 frames Cell Events: 4172 mean_circ: 0.94687
Ran for 62050 frames Cell Events: 4206 mean_circ: 0.946865
Ran for 63050 frames Cell Events: 4274 mean_circ: 0.946908
Ran for 64550 frames Cell Events: 4385 mean_circ: 0.946897
Ran for 65050 frames Cell Events: 4418 mean_circ: 0.946816
Ran for 66550 frames Cell Events: 4501 mean_circ: 0.946849
Ran for 68050 frames Cell Events: 4613 mean_circ: 0.946652
Ran for 68550 frames Cell Events: 4638 mean_circ: 0.946671
Ran for 70550 frames Cell Events: 4759 mean_circ: 0.946655
Ran for 74050 frames Cell Events: 5012 mean_circ: 0.946488
Ran for 75550 frames Cell Events: 5115 mean_circ: 0.946535
Ran for 82050 frames Cell Events: 5580 mean_circ: 0.946326
Ran for 84050 frames Cell Events: 5715 mean_circ: 0.94635
Ran for 84550 frames Cell Events: 5739 mean_circ: 0.946365
Ran for 85050 frames Cell Events: 5774 mean_circ: 0.946343
Ran for 85550 frames Cell Events: 5797 mean_circ: 0.946283
Ran for 87050 frames Cell Events: 5897 mean_circ: 0.946322
Ran for 88550 frames Cell Events: 5999 mean_circ: 0.946251
Ran for 91550 frames Cell Events: 6215 mean_circ: 0.946343
Ran for 93050 frames Cell Events: 6311 mean_circ: 0.946304
Ran for 94050 frames Cell Events: 6390 mean_circ: 0.946265
Ran for 95050 frames Cell Events: 6455 mean_circ: 0.946292
Ran for 96550 frames Cell Events: 6548 mean_circ: 0.946344
Ran for 97050 frames Cell Events: 6579 mean_circ: 0.9464
Ran for 97550 frames Cell Events: 6619 mean_circ: 0.9464
Ran for 98050 frames Cell Events: 6653 mean_circ: 0.946387
Ran for 98550 frames Cell Events: 6681 mean_circ: 0.946405
Ran for 99050 frames Cell Events: 6714 mean_circ: 0.946376
Ran for 100550 frames Cell Events: 6821 mean_circ: 0.946438
Ran for 102050 frames Cell Events: 6950 mean_circ: 0.946433
Ran for 103550 frames Cell Events: 7053 mean_circ: 0.946458
Ran for 104550 frames Cell Events: 7127 mean_circ: 0.946492
Ran for 105550 frames Cell Events: 7197 mean_circ: 0.946495
Ran for 106050 frames Cell Events: 7230 mean_circ: 0.946487
Ran for 106550 frames Cell Events: 7269 mean_circ: 0.946486
Ran for 110050 frames Cell Events: 7504 mean_circ: 0.946492
Ran for 112550 frames Cell Events: 7663 mean_circ: 0.946483
Ran for 113050 frames Cell Events: 7697 mean_circ: 0.946512
Ran for 113550 frames Cell Events: 7731 mean_circ: 0.946486
Ran for 115050 frames Cell Events: 7847 mean_circ: 0.946543
Ran for 117050 frames Cell Events: 7970 mean_circ: 0.946451
Ran for 119050 frames Cell Events: 8096 mean_circ: 0.946464
Ran for 120050 frames Cell Events: 8167 mean_circ: 0.946453
Ran for 121050 frames Cell Events: 8251 mean_circ: 0.946528



End this measurment..



Stopping time: 2.54057e+009
****************************************
Measurement finished

Mean Circularitiy: 0.94654
Mean Area: 597.124
Mean FL-1 intensity: 3473.77
Mean FL-2 intensity: 3043.36
Mean FL-3 intensity: 0

frames processed: 119731
Total Time: 60634.2ms
Number of Runs (new bg img) 0
Frames collected: 121131
Skipped frames: 56312
Rescued Frames: 4672996
Samples DAQ: 30284414
Samples written: 60565398
Samples per Channel: 30282699
FPS: 1997.73

Samples per second: 499433
Expected samples/s: 500000

Events: 8261
Events per second: 136.243
jumped back (PMT): 3049
#Frames read, frequency
# 0 frames :197934
# 1 frames :33168
# 2 frames :2322
# 3 frames :827
# 4 frames :477
# 5 frames :272
# 6 frames :1558
# 7 frames :864
# 8 frames :855
# 9 frames :531
# 10 frames :381
# 11 frames :278
# 12 frames :232
# 13 frames :156
# 14 frames :283
# 15 frames :246
# 16 frames :166
# 17 frames :169
# 18 frames :108
# 19 frames :84
# 20 frames :93
# 21 frames :81
# 22 frames :66
# 23 frames :52
# 24 frames :51
# 25 frames :34
# 26 frames :44
# 27 frames :37
# 28 frames :33
# 29 frames :28
# 30 frames :22
# 31 frames :27
# 32 frames :16
# 33 frames :18
# 34 frames :13
# 35 frames :16
# 36 frames :10
# 37 frames :12
# 38 frames :15
# 39 frames :14
# 40 frames :10
# 41 frames :9
# 42 frames :10
# 43 frames :7
# 44 frames :7
# 45 frames :4
# 46 frames :9
# 47 frames :5
# 48 frames :4
# 49 frames :2
# 50 frames :3
# 51 frames :5
# 52 frames :1
# 54 frames :3
# 55 frames :1
# 57 frames :3
# 59 frames :3
# 61 frames :1
# 62 frames :2
# 63 frames :2
# 64 frames :1
# 66 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7807
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0