Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.26.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5c31c3c55c0b26cbb6110900124dacd1",
"name": "M1_20170613_1807_dsRNA_X7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:26:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8496 (max 8497) in '8496'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7685
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54066e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.946528
Ran for 550 frames Cell Events: 23 mean_circ: 0.931068
Ran for 2550 frames Cell Events: 158 mean_circ: 0.939212
Ran for 5050 frames Cell Events: 337 mean_circ: 0.937745
Ran for 6050 frames Cell Events: 403 mean_circ: 0.93734
Ran for 7050 frames Cell Events: 485 mean_circ: 0.93535
Ran for 8050 frames Cell Events: 550 mean_circ: 0.93633
Ran for 8550 frames Cell Events: 590 mean_circ: 0.936308
Ran for 9050 frames Cell Events: 618 mean_circ: 0.93688
Ran for 12550 frames Cell Events: 848 mean_circ: 0.936028
Ran for 13550 frames Cell Events: 908 mean_circ: 0.935737
Ran for 15050 frames Cell Events: 1020 mean_circ: 0.935682
Ran for 15550 frames Cell Events: 1045 mean_circ: 0.935726
Ran for 18050 frames Cell Events: 1240 mean_circ: 0.935633
Ran for 18550 frames Cell Events: 1270 mean_circ: 0.935617
Ran for 25050 frames Cell Events: 1776 mean_circ: 0.935883
Ran for 26550 frames Cell Events: 1885 mean_circ: 0.935476
Ran for 27050 frames Cell Events: 1923 mean_circ: 0.935389
Ran for 28050 frames Cell Events: 1998 mean_circ: 0.935584
Ran for 29050 frames Cell Events: 2071 mean_circ: 0.935584
Ran for 29550 frames Cell Events: 2096 mean_circ: 0.935285
Ran for 30050 frames Cell Events: 2136 mean_circ: 0.935285
Ran for 30550 frames Cell Events: 2167 mean_circ: 0.935222
Ran for 33050 frames Cell Events: 2335 mean_circ: 0.935173
Ran for 36050 frames Cell Events: 2543 mean_circ: 0.935129
Ran for 36550 frames Cell Events: 2561 mean_circ: 0.935111
Ran for 38050 frames Cell Events: 2661 mean_circ: 0.93514
Ran for 38550 frames Cell Events: 2694 mean_circ: 0.935269
Ran for 41050 frames Cell Events: 2886 mean_circ: 0.935442
Ran for 41550 frames Cell Events: 2914 mean_circ: 0.935369
Ran for 45550 frames Cell Events: 3160 mean_circ: 0.935563
Ran for 46050 frames Cell Events: 3186 mean_circ: 0.935631
Ran for 47550 frames Cell Events: 3307 mean_circ: 0.935676
Ran for 49550 frames Cell Events: 3424 mean_circ: 0.935639
Ran for 50550 frames Cell Events: 3515 mean_circ: 0.935655
Ran for 51550 frames Cell Events: 3587 mean_circ: 0.93579
Ran for 52050 frames Cell Events: 3613 mean_circ: 0.935739
Ran for 52550 frames Cell Events: 3645 mean_circ: 0.935687
Ran for 55050 frames Cell Events: 3851 mean_circ: 0.935656
Ran for 59050 frames Cell Events: 4175 mean_circ: 0.935894
Ran for 59550 frames Cell Events: 4221 mean_circ: 0.935894
Ran for 61050 frames Cell Events: 4342 mean_circ: 0.935962
Ran for 62550 frames Cell Events: 4453 mean_circ: 0.935882
Ran for 63050 frames Cell Events: 4471 mean_circ: 0.935873
Ran for 64050 frames Cell Events: 4538 mean_circ: 0.935823
Ran for 70050 frames Cell Events: 5008 mean_circ: 0.935791
Ran for 73050 frames Cell Events: 5230 mean_circ: 0.935954
Ran for 75550 frames Cell Events: 5430 mean_circ: 0.936172
Ran for 77050 frames Cell Events: 5543 mean_circ: 0.936219
Ran for 77550 frames Cell Events: 5583 mean_circ: 0.936238
Ran for 82050 frames Cell Events: 5923 mean_circ: 0.936376
Ran for 82550 frames Cell Events: 5972 mean_circ: 0.936361
Ran for 83050 frames Cell Events: 6014 mean_circ: 0.936361
Ran for 84550 frames Cell Events: 6142 mean_circ: 0.936411
Ran for 88050 frames Cell Events: 6435 mean_circ: 0.936299
Ran for 88550 frames Cell Events: 6464 mean_circ: 0.936265
Ran for 90050 frames Cell Events: 6568 mean_circ: 0.936178
Ran for 92550 frames Cell Events: 6757 mean_circ: 0.936208
Ran for 94550 frames Cell Events: 6912 mean_circ: 0.93622
Ran for 96550 frames Cell Events: 7063 mean_circ: 0.936214
Ran for 99050 frames Cell Events: 7239 mean_circ: 0.93622
Ran for 99550 frames Cell Events: 7271 mean_circ: 0.936237
Ran for 101050 frames Cell Events: 7396 mean_circ: 0.93621
Ran for 102050 frames Cell Events: 7481 mean_circ: 0.936239
Ran for 108050 frames Cell Events: 7948 mean_circ: 0.936298
Ran for 108550 frames Cell Events: 7976 mean_circ: 0.936315
Ran for 109550 frames Cell Events: 8064 mean_circ: 0.936228
Ran for 112050 frames Cell Events: 8263 mean_circ: 0.936201



End this measurment..



Stopping time: 2.54072e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93587
Mean Area: 590.793
Mean FL-1 intensity: 3239.06
Mean FL-2 intensity: 3217.24
Mean FL-3 intensity: 0

frames processed: 110579
Total Time: 57477.1ms
Number of Runs (new bg img) 0
Frames collected: 114796
Skipped frames: 60529
Rescued Frames: 4751451
Samples DAQ: 28700670
Samples written: 57397900
Samples per Channel: 28698950
FPS: 1997.25

Samples per second: 499311
Expected samples/s: 500000

Events: 8497
Events per second: 147.833
jumped back (PMT): 40
#Frames read, frequency
# 0 frames :163013
# 1 frames :22606
# 2 frames :1952
# 3 frames :672
# 4 frames :482
# 5 frames :230
# 6 frames :1012
# 7 frames :819
# 8 frames :626
# 9 frames :521
# 10 frames :581
# 11 frames :304
# 12 frames :222
# 13 frames :163
# 14 frames :166
# 15 frames :227
# 16 frames :176
# 17 frames :146
# 18 frames :115
# 19 frames :117
# 20 frames :79
# 21 frames :73
# 22 frames :91
# 23 frames :70
# 24 frames :62
# 25 frames :65
# 26 frames :50
# 27 frames :51
# 28 frames :47
# 29 frames :37
# 30 frames :35
# 31 frames :39
# 32 frames :34
# 33 frames :25
# 34 frames :20
# 35 frames :29
# 36 frames :18
# 37 frames :14
# 38 frames :17
# 39 frames :17
# 40 frames :19
# 41 frames :21
# 42 frames :10
# 43 frames :11
# 44 frames :12
# 45 frames :12
# 46 frames :8
# 47 frames :4
# 48 frames :6
# 49 frames :6
# 50 frames :5
# 51 frames :10
# 52 frames :5
# 53 frames :5
# 54 frames :2
# 55 frames :3
# 56 frames :2
# 58 frames :2
# 60 frames :1
# 61 frames :2
# 62 frames :3
# 63 frames :3
# 64 frames :4
# 66 frames :3
# 67 frames :1
# 68 frames :3
# 69 frames :1
# 70 frames :3
# 72 frames :1
# 74 frames :1
# 76 frames :1
# 78 frames :1
# 79 frames :1
# 85 frames :1
# 91 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7685
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0