Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.26.58 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "80824043de5cd2ab73c6afd4c9c24894",
"name": "M1_20170613_1818_dsRNA_X3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:27:19"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8345 (max 8346) in '8345'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8188
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54132e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 41 mean_circ: 0.947714
Ran for 1550 frames Cell Events: 55 mean_circ: 0.945966
Ran for 2050 frames Cell Events: 92 mean_circ: 0.945977
Ran for 2550 frames Cell Events: 116 mean_circ: 0.942211
Ran for 3050 frames Cell Events: 154 mean_circ: 0.936427
Ran for 4550 frames Cell Events: 221 mean_circ: 0.938829
Ran for 5050 frames Cell Events: 244 mean_circ: 0.938423
Ran for 5550 frames Cell Events: 268 mean_circ: 0.938896
Ran for 6050 frames Cell Events: 290 mean_circ: 0.938182
Ran for 8050 frames Cell Events: 356 mean_circ: 0.93841
Ran for 8550 frames Cell Events: 387 mean_circ: 0.938204
Ran for 9050 frames Cell Events: 412 mean_circ: 0.936839
Ran for 9550 frames Cell Events: 446 mean_circ: 0.937172
Ran for 12050 frames Cell Events: 591 mean_circ: 0.936917
Ran for 14050 frames Cell Events: 702 mean_circ: 0.938375
Ran for 16550 frames Cell Events: 838 mean_circ: 0.93735
Ran for 18550 frames Cell Events: 937 mean_circ: 0.93718
Ran for 20050 frames Cell Events: 1015 mean_circ: 0.937091
Ran for 22050 frames Cell Events: 1131 mean_circ: 0.937021
Ran for 24050 frames Cell Events: 1227 mean_circ: 0.936526
Ran for 24550 frames Cell Events: 1256 mean_circ: 0.936635
Ran for 25050 frames Cell Events: 1274 mean_circ: 0.936788
Ran for 26050 frames Cell Events: 1315 mean_circ: 0.936523
Ran for 27050 frames Cell Events: 1363 mean_circ: 0.936781
Ran for 27550 frames Cell Events: 1388 mean_circ: 0.936815
Ran for 28050 frames Cell Events: 1407 mean_circ: 0.936681
Ran for 28550 frames Cell Events: 1427 mean_circ: 0.936652
Ran for 29550 frames Cell Events: 1466 mean_circ: 0.936838
Ran for 30550 frames Cell Events: 1511 mean_circ: 0.936367
Ran for 31050 frames Cell Events: 1543 mean_circ: 0.936429
Ran for 32050 frames Cell Events: 1582 mean_circ: 0.936495
Ran for 32550 frames Cell Events: 1607 mean_circ: 0.936662
Ran for 33050 frames Cell Events: 1635 mean_circ: 0.936544
Ran for 33550 frames Cell Events: 1663 mean_circ: 0.936448
Ran for 34050 frames Cell Events: 1683 mean_circ: 0.936221
Ran for 35550 frames Cell Events: 1758 mean_circ: 0.935968
Ran for 36050 frames Cell Events: 1780 mean_circ: 0.935786
Ran for 36550 frames Cell Events: 1801 mean_circ: 0.935895
Ran for 37550 frames Cell Events: 1840 mean_circ: 0.936077
Ran for 38550 frames Cell Events: 1880 mean_circ: 0.935984
Ran for 41050 frames Cell Events: 2013 mean_circ: 0.935905
Ran for 41550 frames Cell Events: 2032 mean_circ: 0.935722
Ran for 42050 frames Cell Events: 2065 mean_circ: 0.936041
Ran for 42550 frames Cell Events: 2090 mean_circ: 0.936041
Ran for 43050 frames Cell Events: 2113 mean_circ: 0.935966
Ran for 43550 frames Cell Events: 2136 mean_circ: 0.935933
Ran for 46550 frames Cell Events: 2283 mean_circ: 0.935909
Ran for 48050 frames Cell Events: 2364 mean_circ: 0.935903
Ran for 48550 frames Cell Events: 2382 mean_circ: 0.935907
Ran for 49050 frames Cell Events: 2404 mean_circ: 0.935965
Ran for 50050 frames Cell Events: 2441 mean_circ: 0.935873
Ran for 51050 frames Cell Events: 2504 mean_circ: 0.936036
Ran for 51550 frames Cell Events: 2540 mean_circ: 0.93598
Ran for 52050 frames Cell Events: 2572 mean_circ: 0.935945
Ran for 52550 frames Cell Events: 2595 mean_circ: 0.936015
Ran for 53050 frames Cell Events: 2612 mean_circ: 0.935999
Ran for 53550 frames Cell Events: 2640 mean_circ: 0.93586
Ran for 54050 frames Cell Events: 2662 mean_circ: 0.93585
Ran for 54550 frames Cell Events: 2697 mean_circ: 0.935837
Ran for 56050 frames Cell Events: 2771 mean_circ: 0.935661
Ran for 56550 frames Cell Events: 2790 mean_circ: 0.935756
Ran for 57550 frames Cell Events: 2826 mean_circ: 0.93575
Ran for 58050 frames Cell Events: 2846 mean_circ: 0.935689
Ran for 59050 frames Cell Events: 2894 mean_circ: 0.935685
Ran for 59550 frames Cell Events: 2914 mean_circ: 0.935768
Ran for 60550 frames Cell Events: 2947 mean_circ: 0.935707
Ran for 62050 frames Cell Events: 3008 mean_circ: 0.935715
Ran for 63050 frames Cell Events: 3051 mean_circ: 0.935838
Ran for 63550 frames Cell Events: 3069 mean_circ: 0.935862
Ran for 64050 frames Cell Events: 3095 mean_circ: 0.935955
Ran for 64550 frames Cell Events: 3123 mean_circ: 0.935936
Ran for 65550 frames Cell Events: 3190 mean_circ: 0.936064
Ran for 66050 frames Cell Events: 3220 mean_circ: 0.936006
Ran for 66550 frames Cell Events: 3247 mean_circ: 0.936045
Ran for 67550 frames Cell Events: 3288 mean_circ: 0.936019
Ran for 69550 frames Cell Events: 3384 mean_circ: 0.936223
Ran for 70050 frames Cell Events: 3412 mean_circ: 0.936161
Ran for 70550 frames Cell Events: 3443 mean_circ: 0.936077
Ran for 71050 frames Cell Events: 3464 mean_circ: 0.936146
Ran for 73050 frames Cell Events: 3574 mean_circ: 0.936341
Ran for 73550 frames Cell Events: 3610 mean_circ: 0.936298
Ran for 76050 frames Cell Events: 3787 mean_circ: 0.936281
Ran for 76550 frames Cell Events: 3804 mean_circ: 0.936312
Ran for 77050 frames Cell Events: 3831 mean_circ: 0.936387
Ran for 77550 frames Cell Events: 3863 mean_circ: 0.936484
Ran for 78050 frames Cell Events: 3894 mean_circ: 0.936344
Ran for 79550 frames Cell Events: 3975 mean_circ: 0.936171
Ran for 81050 frames Cell Events: 4046 mean_circ: 0.936129
Ran for 81550 frames Cell Events: 4071 mean_circ: 0.936127
Ran for 82550 frames Cell Events: 4113 mean_circ: 0.936175
Ran for 83550 frames Cell Events: 4173 mean_circ: 0.936206
Ran for 84050 frames Cell Events: 4201 mean_circ: 0.936165
Ran for 84550 frames Cell Events: 4225 mean_circ: 0.936188
Ran for 85050 frames Cell Events: 4260 mean_circ: 0.936238
Ran for 85550 frames Cell Events: 4290 mean_circ: 0.936273
Ran for 87050 frames Cell Events: 4379 mean_circ: 0.936083
Ran for 87550 frames Cell Events: 4393 mean_circ: 0.936152
Ran for 88050 frames Cell Events: 4427 mean_circ: 0.936097
Ran for 89050 frames Cell Events: 4480 mean_circ: 0.936172
Ran for 91050 frames Cell Events: 4594 mean_circ: 0.936259
Ran for 91550 frames Cell Events: 4618 mean_circ: 0.936263
Ran for 92550 frames Cell Events: 4670 mean_circ: 0.936248
Ran for 93050 frames Cell Events: 4703 mean_circ: 0.936193
Ran for 94050 frames Cell Events: 4749 mean_circ: 0.936153
Ran for 94550 frames Cell Events: 4785 mean_circ: 0.936174
Ran for 96050 frames Cell Events: 4853 mean_circ: 0.936062
Ran for 96550 frames Cell Events: 4881 mean_circ: 0.936126
Ran for 97050 frames Cell Events: 4899 mean_circ: 0.936128
Ran for 97550 frames Cell Events: 4937 mean_circ: 0.936128
Ran for 98050 frames Cell Events: 4953 mean_circ: 0.936062
Ran for 98550 frames Cell Events: 4981 mean_circ: 0.936076
Ran for 99050 frames Cell Events: 5009 mean_circ: 0.936154
Ran for 100050 frames Cell Events: 5067 mean_circ: 0.936182
Ran for 100550 frames Cell Events: 5092 mean_circ: 0.936149
Ran for 101550 frames Cell Events: 5151 mean_circ: 0.936312
Ran for 106550 frames Cell Events: 5378 mean_circ: 0.936388
Ran for 107550 frames Cell Events: 5432 mean_circ: 0.936305
Ran for 108050 frames Cell Events: 5457 mean_circ: 0.936231
Ran for 109050 frames Cell Events: 5495 mean_circ: 0.936246
Ran for 109550 frames Cell Events: 5524 mean_circ: 0.936206
Ran for 111550 frames Cell Events: 5619 mean_circ: 0.936175
Ran for 113050 frames Cell Events: 5704 mean_circ: 0.936154
Ran for 113550 frames Cell Events: 5730 mean_circ: 0.936154
Ran for 114050 frames Cell Events: 5758 mean_circ: 0.936191
Ran for 115050 frames Cell Events: 5810 mean_circ: 0.936152
Ran for 115550 frames Cell Events: 5832 mean_circ: 0.936169
Ran for 116050 frames Cell Events: 5860 mean_circ: 0.936205
Ran for 117050 frames Cell Events: 5904 mean_circ: 0.936247
Ran for 117550 frames Cell Events: 5927 mean_circ: 0.93626
Ran for 119050 frames Cell Events: 5990 mean_circ: 0.936235
Ran for 120050 frames Cell Events: 6053 mean_circ: 0.936259
Ran for 124550 frames Cell Events: 6273 mean_circ: 0.93606
Ran for 125050 frames Cell Events: 6294 mean_circ: 0.936096
Ran for 126050 frames Cell Events: 6354 mean_circ: 0.935993
Ran for 127050 frames Cell Events: 6412 mean_circ: 0.936
Ran for 128050 frames Cell Events: 6456 mean_circ: 0.936048
Ran for 129050 frames Cell Events: 6509 mean_circ: 0.936068
Ran for 131050 frames Cell Events: 6621 mean_circ: 0.936075
Ran for 132050 frames Cell Events: 6678 mean_circ: 0.936067
Ran for 133050 frames Cell Events: 6715 mean_circ: 0.936095
Ran for 134050 frames Cell Events: 6772 mean_circ: 0.936163
Ran for 134550 frames Cell Events: 6786 mean_circ: 0.936149
Ran for 135050 frames Cell Events: 6807 mean_circ: 0.936159
Ran for 136050 frames Cell Events: 6859 mean_circ: 0.936123
Ran for 137050 frames Cell Events: 6920 mean_circ: 0.936129
Ran for 139050 frames Cell Events: 7016 mean_circ: 0.936183
Ran for 140050 frames Cell Events: 7066 mean_circ: 0.936197
Ran for 141050 frames Cell Events: 7120 mean_circ: 0.936232
Ran for 141550 frames Cell Events: 7144 mean_circ: 0.936248
Ran for 142050 frames Cell Events: 7164 mean_circ: 0.936281
Ran for 143550 frames Cell Events: 7243 mean_circ: 0.936329
Ran for 144050 frames Cell Events: 7259 mean_circ: 0.936283
Ran for 144550 frames Cell Events: 7288 mean_circ: 0.936282
Ran for 147050 frames Cell Events: 7441 mean_circ: 0.9363
Ran for 147550 frames Cell Events: 7472 mean_circ: 0.93625
Ran for 148050 frames Cell Events: 7496 mean_circ: 0.93623
Ran for 148550 frames Cell Events: 7519 mean_circ: 0.936202
Ran for 150050 frames Cell Events: 7613 mean_circ: 0.936222
Ran for 151550 frames Cell Events: 7691 mean_circ: 0.936228
Ran for 153050 frames Cell Events: 7806 mean_circ: 0.936088
Ran for 153550 frames Cell Events: 7834 mean_circ: 0.936083
Ran for 154550 frames Cell Events: 7909 mean_circ: 0.935951
Ran for 155050 frames Cell Events: 7935 mean_circ: 0.935985
Ran for 155550 frames Cell Events: 7963 mean_circ: 0.935986
Ran for 156050 frames Cell Events: 7979 mean_circ: 0.935948
Ran for 156550 frames Cell Events: 8013 mean_circ: 0.935905
Ran for 157050 frames Cell Events: 8035 mean_circ: 0.935929
Ran for 160550 frames Cell Events: 8230 mean_circ: 0.936001
Ran for 161050 frames Cell Events: 8253 mean_circ: 0.936001



End this measurment..



Stopping time: 2.5414e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936014
Mean Area: 560.241
Mean FL-1 intensity: 2532.06
Mean FL-2 intensity: 3243.09
Mean FL-3 intensity: 0

frames processed: 161547
Total Time: 81536.9ms
Number of Runs (new bg img) 0
Frames collected: 162957
Skipped frames: 62383
Rescued Frames: 4922634
Samples DAQ: 40740135
Samples written: 81478380
Samples per Channel: 40739190
FPS: 1998.57

Samples per second: 499641
Expected samples/s: 500000

Events: 8346
Events per second: 102.359
jumped back (PMT): 30
#Frames read, frequency
# 0 frames :246729
# 1 frames :64663
# 2 frames :3860
# 3 frames :1178
# 4 frames :734
# 5 frames :327
# 6 frames :1942
# 7 frames :1281
# 8 frames :1523
# 9 frames :791
# 10 frames :541
# 11 frames :379
# 12 frames :302
# 13 frames :187
# 14 frames :244
# 15 frames :209
# 16 frames :164
# 17 frames :112
# 18 frames :93
# 19 frames :65
# 20 frames :62
# 21 frames :60
# 22 frames :46
# 23 frames :36
# 24 frames :47
# 25 frames :30
# 26 frames :22
# 27 frames :18
# 28 frames :19
# 29 frames :9
# 30 frames :14
# 31 frames :11
# 32 frames :6
# 33 frames :11
# 34 frames :10
# 35 frames :4
# 36 frames :4
# 37 frames :3
# 38 frames :4
# 41 frames :4
# 42 frames :2
# 43 frames :5
# 44 frames :6
# 45 frames :2
# 46 frames :5
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :2
# 54 frames :2
# 58 frames :1
# 59 frames :1
# 62 frames :1
# 63 frames :1
# 70 frames :1
# 71 frames :1
# 72 frames :1
# 73 frames :1
# 76 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8188
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0