Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.27.59 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c79e622d129725122c6b417feb1d2eb9",
"name": "M1_20170613_1831_dsRNA_Y6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:28:21"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7906
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54211e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.934747
Ran for 550 frames Cell Events: 27 mean_circ: 0.886475
Ran for 1050 frames Cell Events: 50 mean_circ: 0.9151
Ran for 2050 frames Cell Events: 92 mean_circ: 0.9234
Ran for 3050 frames Cell Events: 157 mean_circ: 0.923184
Ran for 4050 frames Cell Events: 227 mean_circ: 0.925276
Ran for 5050 frames Cell Events: 292 mean_circ: 0.919512
Ran for 6050 frames Cell Events: 359 mean_circ: 0.920194
Ran for 6550 frames Cell Events: 389 mean_circ: 0.920092
Ran for 7050 frames Cell Events: 408 mean_circ: 0.920761
Ran for 7550 frames Cell Events: 425 mean_circ: 0.921233
Ran for 8550 frames Cell Events: 470 mean_circ: 0.921799
Ran for 9550 frames Cell Events: 521 mean_circ: 0.922719
Ran for 11050 frames Cell Events: 588 mean_circ: 0.921547
Ran for 11550 frames Cell Events: 613 mean_circ: 0.921941
Ran for 12550 frames Cell Events: 663 mean_circ: 0.921721
Ran for 13050 frames Cell Events: 703 mean_circ: 0.921721
Ran for 15050 frames Cell Events: 831 mean_circ: 0.921847
Ran for 15550 frames Cell Events: 854 mean_circ: 0.921968
Ran for 16050 frames Cell Events: 890 mean_circ: 0.921914
Ran for 17050 frames Cell Events: 946 mean_circ: 0.922335
Ran for 17550 frames Cell Events: 964 mean_circ: 0.921746
Ran for 18050 frames Cell Events: 987 mean_circ: 0.92187
Ran for 19550 frames Cell Events: 1064 mean_circ: 0.921733
Ran for 20050 frames Cell Events: 1094 mean_circ: 0.921574
Ran for 21050 frames Cell Events: 1162 mean_circ: 0.922206
Ran for 22050 frames Cell Events: 1217 mean_circ: 0.922604
Ran for 23550 frames Cell Events: 1302 mean_circ: 0.922893
Ran for 24050 frames Cell Events: 1326 mean_circ: 0.922694
Ran for 24550 frames Cell Events: 1343 mean_circ: 0.922861
Ran for 25050 frames Cell Events: 1363 mean_circ: 0.922667
Ran for 25550 frames Cell Events: 1386 mean_circ: 0.922371
Ran for 26050 frames Cell Events: 1421 mean_circ: 0.922299
Ran for 27050 frames Cell Events: 1476 mean_circ: 0.921659
Ran for 27550 frames Cell Events: 1496 mean_circ: 0.921554
Ran for 28550 frames Cell Events: 1532 mean_circ: 0.92198
Ran for 29550 frames Cell Events: 1590 mean_circ: 0.921614
Ran for 30050 frames Cell Events: 1619 mean_circ: 0.921498
Ran for 30550 frames Cell Events: 1639 mean_circ: 0.921383
Ran for 31050 frames Cell Events: 1666 mean_circ: 0.921265
Ran for 31550 frames Cell Events: 1693 mean_circ: 0.921281
Ran for 32050 frames Cell Events: 1709 mean_circ: 0.921367
Ran for 32550 frames Cell Events: 1736 mean_circ: 0.921411
Ran for 33550 frames Cell Events: 1772 mean_circ: 0.921618
Ran for 34550 frames Cell Events: 1819 mean_circ: 0.92187
Ran for 35050 frames Cell Events: 1846 mean_circ: 0.921753
Ran for 36550 frames Cell Events: 1906 mean_circ: 0.921887
Ran for 37050 frames Cell Events: 1933 mean_circ: 0.921978
Ran for 38050 frames Cell Events: 1995 mean_circ: 0.921642
Ran for 39550 frames Cell Events: 2054 mean_circ: 0.921815
Ran for 40050 frames Cell Events: 2066 mean_circ: 0.921818
Ran for 40550 frames Cell Events: 2090 mean_circ: 0.921853
Ran for 41550 frames Cell Events: 2138 mean_circ: 0.922088
Ran for 42050 frames Cell Events: 2165 mean_circ: 0.922141
Ran for 42550 frames Cell Events: 2190 mean_circ: 0.92199
Ran for 43050 frames Cell Events: 2216 mean_circ: 0.922336
Ran for 43550 frames Cell Events: 2254 mean_circ: 0.922368
Ran for 44550 frames Cell Events: 2302 mean_circ: 0.922302
Ran for 45550 frames Cell Events: 2340 mean_circ: 0.922563
Ran for 47050 frames Cell Events: 2427 mean_circ: 0.922353
Ran for 50050 frames Cell Events: 2613 mean_circ: 0.922496
Ran for 50550 frames Cell Events: 2641 mean_circ: 0.92263
Ran for 51550 frames Cell Events: 2680 mean_circ: 0.922466
Ran for 52050 frames Cell Events: 2705 mean_circ: 0.922469
Ran for 52550 frames Cell Events: 2727 mean_circ: 0.922722
Ran for 53550 frames Cell Events: 2782 mean_circ: 0.922964
Ran for 54050 frames Cell Events: 2815 mean_circ: 0.923002
Ran for 54550 frames Cell Events: 2840 mean_circ: 0.92318
Ran for 55550 frames Cell Events: 2906 mean_circ: 0.923035
Ran for 60550 frames Cell Events: 3240 mean_circ: 0.922848
Ran for 62050 frames Cell Events: 3346 mean_circ: 0.9229
Ran for 63050 frames Cell Events: 3400 mean_circ: 0.922898
Ran for 64050 frames Cell Events: 3475 mean_circ: 0.923016
Ran for 65050 frames Cell Events: 3540 mean_circ: 0.92292
Ran for 65550 frames Cell Events: 3569 mean_circ: 0.922888
Ran for 66550 frames Cell Events: 3620 mean_circ: 0.922741
Ran for 67050 frames Cell Events: 3642 mean_circ: 0.922607
Ran for 68550 frames Cell Events: 3717 mean_circ: 0.922798
Ran for 71550 frames Cell Events: 3899 mean_circ: 0.922631
Ran for 73050 frames Cell Events: 4009 mean_circ: 0.922666
Ran for 74550 frames Cell Events: 4100 mean_circ: 0.922646
Ran for 75050 frames Cell Events: 4129 mean_circ: 0.922554
Ran for 76050 frames Cell Events: 4182 mean_circ: 0.922597
Ran for 77050 frames Cell Events: 4235 mean_circ: 0.922643
Ran for 77550 frames Cell Events: 4256 mean_circ: 0.922696
Ran for 78050 frames Cell Events: 4269 mean_circ: 0.922707
Ran for 79050 frames Cell Events: 4331 mean_circ: 0.922734
Ran for 79550 frames Cell Events: 4353 mean_circ: 0.922814
Ran for 81050 frames Cell Events: 4441 mean_circ: 0.923016
Ran for 83050 frames Cell Events: 4578 mean_circ: 0.923262
Ran for 84050 frames Cell Events: 4643 mean_circ: 0.923244
Ran for 87050 frames Cell Events: 4854 mean_circ: 0.923314
Ran for 87550 frames Cell Events: 4887 mean_circ: 0.923263
Ran for 90050 frames Cell Events: 5023 mean_circ: 0.923065
Ran for 90550 frames Cell Events: 5034 mean_circ: 0.923056
Ran for 92550 frames Cell Events: 5136 mean_circ: 0.923108
Ran for 93550 frames Cell Events: 5188 mean_circ: 0.923156
Ran for 94050 frames Cell Events: 5216 mean_circ: 0.923146
Ran for 94550 frames Cell Events: 5235 mean_circ: 0.923044
Ran for 95050 frames Cell Events: 5253 mean_circ: 0.923088
Ran for 98550 frames Cell Events: 5426 mean_circ: 0.923054
Ran for 99050 frames Cell Events: 5447 mean_circ: 0.923129
Ran for 99550 frames Cell Events: 5471 mean_circ: 0.923126
Ran for 100050 frames Cell Events: 5495 mean_circ: 0.923091
Ran for 100550 frames Cell Events: 5518 mean_circ: 0.923115
Ran for 101550 frames Cell Events: 5560 mean_circ: 0.92307
Ran for 102050 frames Cell Events: 5574 mean_circ: 0.923067
Ran for 103050 frames Cell Events: 5637 mean_circ: 0.92318
Ran for 103550 frames Cell Events: 5667 mean_circ: 0.923188
Ran for 107550 frames Cell Events: 5871 mean_circ: 0.923078
Ran for 108050 frames Cell Events: 5887 mean_circ: 0.923122
Ran for 109550 frames Cell Events: 5974 mean_circ: 0.92325
Ran for 111050 frames Cell Events: 6081 mean_circ: 0.923234
Ran for 112050 frames Cell Events: 6137 mean_circ: 0.923316
Ran for 112550 frames Cell Events: 6153 mean_circ: 0.923338
Ran for 113050 frames Cell Events: 6164 mean_circ: 0.923366
Ran for 114550 frames Cell Events: 6236 mean_circ: 0.923424
Ran for 117050 frames Cell Events: 6354 mean_circ: 0.923486
Ran for 117550 frames Cell Events: 6365 mean_circ: 0.923461
Ran for 118550 frames Cell Events: 6409 mean_circ: 0.923418
Ran for 119050 frames Cell Events: 6420 mean_circ: 0.923396
Ran for 120550 frames Cell Events: 6476 mean_circ: 0.923434
Ran for 121550 frames Cell Events: 6536 mean_circ: 0.923476
Ran for 122550 frames Cell Events: 6601 mean_circ: 0.923357
Ran for 124050 frames Cell Events: 6689 mean_circ: 0.923368
Ran for 125050 frames Cell Events: 6745 mean_circ: 0.923452
Ran for 125550 frames Cell Events: 6769 mean_circ: 0.923441
Ran for 126050 frames Cell Events: 6801 mean_circ: 0.923367
Ran for 126550 frames Cell Events: 6832 mean_circ: 0.923429
Ran for 127050 frames Cell Events: 6848 mean_circ: 0.92343
Ran for 128550 frames Cell Events: 6944 mean_circ: 0.923462
Ran for 129050 frames Cell Events: 6972 mean_circ: 0.923422
Ran for 129550 frames Cell Events: 6999 mean_circ: 0.92345
Ran for 130550 frames Cell Events: 7058 mean_circ: 0.923447
Ran for 131050 frames Cell Events: 7082 mean_circ: 0.923403
Ran for 132550 frames Cell Events: 7192 mean_circ: 0.923309
Ran for 133050 frames Cell Events: 7235 mean_circ: 0.923312
Ran for 133550 frames Cell Events: 7277 mean_circ: 0.923283
Ran for 134550 frames Cell Events: 7334 mean_circ: 0.923272
Ran for 135050 frames Cell Events: 7357 mean_circ: 0.923195
Ran for 136550 frames Cell Events: 7466 mean_circ: 0.923263
Ran for 137050 frames Cell Events: 7486 mean_circ: 0.92328
Ran for 138050 frames Cell Events: 7566 mean_circ: 0.923216
Ran for 138550 frames Cell Events: 7601 mean_circ: 0.923197
Ran for 139550 frames Cell Events: 7659 mean_circ: 0.923189
Ran for 140550 frames Cell Events: 7726 mean_circ: 0.923173
Ran for 142050 frames Cell Events: 7800 mean_circ: 0.923137
Ran for 143050 frames Cell Events: 7866 mean_circ: 0.923142
Ran for 144050 frames Cell Events: 7937 mean_circ: 0.923173
Ran for 144550 frames Cell Events: 7965 mean_circ: 0.923184
Ran for 145050 frames Cell Events: 7988 mean_circ: 0.923222
Ran for 146050 frames Cell Events: 8054 mean_circ: 0.923233
Ran for 146550 frames Cell Events: 8081 mean_circ: 0.923282
Ran for 148550 frames Cell Events: 8211 mean_circ: 0.923324
Ran for 149550 frames Cell Events: 8258 mean_circ: 0.923303
Ran for 150050 frames Cell Events: 8296 mean_circ: 0.923273
Ran for 151050 frames Cell Events: 8365 mean_circ: 0.923343
Ran for 153050 frames Cell Events: 8468 mean_circ: 0.923342
Ran for 154050 frames Cell Events: 8547 mean_circ: 0.923338
Ran for 155550 frames Cell Events: 8637 mean_circ: 0.923325
Ran for 156050 frames Cell Events: 8652 mean_circ: 0.923361
Ran for 156550 frames Cell Events: 8669 mean_circ: 0.923397
Ran for 157550 frames Cell Events: 8702 mean_circ: 0.923391



End this measurment..



Stopping time: 2.54219e+009
****************************************
Measurement finished

Mean Circularitiy: 0.923288
Mean Area: 645.128
Mean FL-1 intensity: 3382.74
Mean FL-2 intensity: 3860.26
Mean FL-3 intensity: 0

frames processed: 157572
Total Time: 79627.4ms
Number of Runs (new bg img) 0
Frames collected: 159111
Skipped frames: 64938
Rescued Frames: 5125684
Samples DAQ: 39781875
Samples written: 79555166
Samples per Channel: 39777583
FPS: 1998.2

Samples per second: 499547
Expected samples/s: 500000

Events: 8795
Events per second: 110.452
jumped back (PMT): 34
#Frames read, frequency
# 0 frames :252825
# 1 frames :51484
# 2 frames :3158
# 3 frames :1255
# 4 frames :813
# 5 frames :453
# 6 frames :1473
# 7 frames :1186
# 8 frames :905
# 9 frames :629
# 10 frames :754
# 11 frames :634
# 12 frames :419
# 13 frames :224
# 14 frames :219
# 15 frames :230
# 16 frames :176
# 17 frames :183
# 18 frames :132
# 19 frames :102
# 20 frames :99
# 21 frames :77
# 22 frames :80
# 23 frames :70
# 24 frames :64
# 25 frames :46
# 26 frames :43
# 27 frames :34
# 28 frames :35
# 29 frames :42
# 30 frames :19
# 31 frames :15
# 32 frames :12
# 33 frames :18
# 34 frames :19
# 35 frames :12
# 36 frames :11
# 37 frames :12
# 38 frames :12
# 39 frames :9
# 40 frames :5
# 41 frames :8
# 42 frames :7
# 43 frames :6
# 44 frames :1
# 45 frames :10
# 46 frames :3
# 47 frames :9
# 48 frames :3
# 49 frames :4
# 50 frames :3
# 51 frames :4
# 52 frames :2
# 53 frames :2
# 54 frames :1
# 55 frames :2
# 57 frames :1
# 58 frames :1
# 60 frames :2
# 64 frames :1
# 65 frames :1
# 67 frames :1
# 68 frames :1
# 69 frames :2
# 78 frames :1
# 80 frames :1
# 81 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7906
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0