Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.28.29 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7d8c6663476cbd54e9437fd9da71589d",
"name": "M1_20170613_1839_dsRNA_Y2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:28:51"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7318
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54257e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.923393
Ran for 1550 frames Cell Events: 63 mean_circ: 0.953024
Ran for 2050 frames Cell Events: 88 mean_circ: 0.948809
Ran for 2550 frames Cell Events: 111 mean_circ: 0.945642
Ran for 8050 frames Cell Events: 415 mean_circ: 0.947874
Ran for 9550 frames Cell Events: 487 mean_circ: 0.948115
Ran for 11550 frames Cell Events: 572 mean_circ: 0.948803
Ran for 12550 frames Cell Events: 632 mean_circ: 0.94839
Ran for 13050 frames Cell Events: 655 mean_circ: 0.948413
Ran for 13550 frames Cell Events: 683 mean_circ: 0.948522
Ran for 17050 frames Cell Events: 880 mean_circ: 0.948128
Ran for 19050 frames Cell Events: 970 mean_circ: 0.947535
Ran for 19550 frames Cell Events: 1001 mean_circ: 0.947418
Ran for 20550 frames Cell Events: 1075 mean_circ: 0.947783
Ran for 21550 frames Cell Events: 1138 mean_circ: 0.947957
Ran for 22050 frames Cell Events: 1174 mean_circ: 0.947676
Ran for 25050 frames Cell Events: 1349 mean_circ: 0.947927
Ran for 25550 frames Cell Events: 1386 mean_circ: 0.947927
Ran for 26550 frames Cell Events: 1438 mean_circ: 0.947775
Ran for 28050 frames Cell Events: 1538 mean_circ: 0.947984
Ran for 31550 frames Cell Events: 1770 mean_circ: 0.947495
Ran for 32050 frames Cell Events: 1803 mean_circ: 0.947535
Ran for 33050 frames Cell Events: 1854 mean_circ: 0.947417
Ran for 33550 frames Cell Events: 1881 mean_circ: 0.947469
Ran for 35050 frames Cell Events: 1958 mean_circ: 0.947501
Ran for 35550 frames Cell Events: 1975 mean_circ: 0.947601
Ran for 36550 frames Cell Events: 2026 mean_circ: 0.947766
Ran for 37050 frames Cell Events: 2051 mean_circ: 0.947829
Ran for 39550 frames Cell Events: 2207 mean_circ: 0.947677
Ran for 40550 frames Cell Events: 2266 mean_circ: 0.947575
Ran for 43050 frames Cell Events: 2418 mean_circ: 0.94768
Ran for 43550 frames Cell Events: 2454 mean_circ: 0.94791
Ran for 44550 frames Cell Events: 2515 mean_circ: 0.947845
Ran for 46050 frames Cell Events: 2609 mean_circ: 0.947866
Ran for 47550 frames Cell Events: 2699 mean_circ: 0.947812
Ran for 48050 frames Cell Events: 2726 mean_circ: 0.947828
Ran for 50050 frames Cell Events: 2836 mean_circ: 0.947834
Ran for 50550 frames Cell Events: 2848 mean_circ: 0.94776
Ran for 52550 frames Cell Events: 2961 mean_circ: 0.947771
Ran for 54550 frames Cell Events: 3105 mean_circ: 0.94768
Ran for 55050 frames Cell Events: 3128 mean_circ: 0.947712
Ran for 57550 frames Cell Events: 3282 mean_circ: 0.947534
Ran for 58050 frames Cell Events: 3309 mean_circ: 0.947534
Ran for 60050 frames Cell Events: 3421 mean_circ: 0.947468
Ran for 60550 frames Cell Events: 3454 mean_circ: 0.947441
Ran for 61550 frames Cell Events: 3517 mean_circ: 0.947443
Ran for 62050 frames Cell Events: 3542 mean_circ: 0.947487
Ran for 62550 frames Cell Events: 3577 mean_circ: 0.947514
Ran for 63050 frames Cell Events: 3608 mean_circ: 0.947624
Ran for 63550 frames Cell Events: 3645 mean_circ: 0.947617
Ran for 66550 frames Cell Events: 3834 mean_circ: 0.947859
Ran for 67050 frames Cell Events: 3855 mean_circ: 0.947787
Ran for 67550 frames Cell Events: 3882 mean_circ: 0.947783
Ran for 68050 frames Cell Events: 3913 mean_circ: 0.947818
Ran for 68550 frames Cell Events: 3941 mean_circ: 0.947809
Ran for 69050 frames Cell Events: 3964 mean_circ: 0.947793
Ran for 70050 frames Cell Events: 4018 mean_circ: 0.947751
Ran for 71050 frames Cell Events: 4076 mean_circ: 0.947574
Ran for 72050 frames Cell Events: 4152 mean_circ: 0.947506
Ran for 73050 frames Cell Events: 4213 mean_circ: 0.947461
Ran for 76550 frames Cell Events: 4402 mean_circ: 0.947388
Ran for 81050 frames Cell Events: 4712 mean_circ: 0.947107
Ran for 81550 frames Cell Events: 4749 mean_circ: 0.947107
Ran for 82050 frames Cell Events: 4776 mean_circ: 0.947029
Ran for 84550 frames Cell Events: 4913 mean_circ: 0.94707
Ran for 85550 frames Cell Events: 4967 mean_circ: 0.947128
Ran for 86050 frames Cell Events: 4993 mean_circ: 0.947063
Ran for 87050 frames Cell Events: 5055 mean_circ: 0.947038
Ran for 87550 frames Cell Events: 5078 mean_circ: 0.947029
Ran for 88050 frames Cell Events: 5110 mean_circ: 0.947029
Ran for 88550 frames Cell Events: 5138 mean_circ: 0.947029
Ran for 89050 frames Cell Events: 5180 mean_circ: 0.946959
Ran for 90550 frames Cell Events: 5266 mean_circ: 0.946993
Ran for 91050 frames Cell Events: 5293 mean_circ: 0.947055
Ran for 94050 frames Cell Events: 5478 mean_circ: 0.946991
Ran for 96050 frames Cell Events: 5587 mean_circ: 0.94687
Ran for 97550 frames Cell Events: 5671 mean_circ: 0.946813
Ran for 98050 frames Cell Events: 5695 mean_circ: 0.946854
Ran for 99550 frames Cell Events: 5797 mean_circ: 0.946888
Ran for 101050 frames Cell Events: 5872 mean_circ: 0.946683
Ran for 102050 frames Cell Events: 5917 mean_circ: 0.94663
Ran for 103550 frames Cell Events: 5997 mean_circ: 0.946699
Ran for 104050 frames Cell Events: 6024 mean_circ: 0.946716
Ran for 104550 frames Cell Events: 6056 mean_circ: 0.94672
Ran for 105050 frames Cell Events: 6094 mean_circ: 0.946724
Ran for 105550 frames Cell Events: 6129 mean_circ: 0.946728
Ran for 106050 frames Cell Events: 6168 mean_circ: 0.946666
Ran for 107050 frames Cell Events: 6235 mean_circ: 0.946653
Ran for 107550 frames Cell Events: 6275 mean_circ: 0.946649
Ran for 108550 frames Cell Events: 6336 mean_circ: 0.94665
Ran for 113550 frames Cell Events: 6674 mean_circ: 0.946629
Ran for 116050 frames Cell Events: 6845 mean_circ: 0.946523
Ran for 116550 frames Cell Events: 6877 mean_circ: 0.94654
Ran for 118050 frames Cell Events: 6986 mean_circ: 0.946626
Ran for 118550 frames Cell Events: 7028 mean_circ: 0.946641
Ran for 124550 frames Cell Events: 7387 mean_circ: 0.946539
Ran for 125550 frames Cell Events: 7465 mean_circ: 0.946497
Ran for 126050 frames Cell Events: 7501 mean_circ: 0.946488
Ran for 126550 frames Cell Events: 7526 mean_circ: 0.946463
Ran for 127050 frames Cell Events: 7563 mean_circ: 0.946439
Ran for 127550 frames Cell Events: 7575 mean_circ: 0.94645
Ran for 128050 frames Cell Events: 7605 mean_circ: 0.94645
Ran for 128550 frames Cell Events: 7625 mean_circ: 0.946447
Ran for 130050 frames Cell Events: 7718 mean_circ: 0.946515
Ran for 131550 frames Cell Events: 7798 mean_circ: 0.946547
Ran for 132550 frames Cell Events: 7866 mean_circ: 0.946531
Ran for 133050 frames Cell Events: 7905 mean_circ: 0.946526
Ran for 136050 frames Cell Events: 8115 mean_circ: 0.946412
Ran for 139050 frames Cell Events: 8325 mean_circ: 0.946362
Ran for 140050 frames Cell Events: 8391 mean_circ: 0.946374
Ran for 142050 frames Cell Events: 8491 mean_circ: 0.946436
Ran for 142550 frames Cell Events: 8511 mean_circ: 0.946456
Ran for 143050 frames Cell Events: 8544 mean_circ: 0.946456
Ran for 143550 frames Cell Events: 8575 mean_circ: 0.946449
Ran for 145050 frames Cell Events: 8671 mean_circ: 0.946446
Ran for 147550 frames Cell Events: 8815 mean_circ: 0.946522
Ran for 149050 frames Cell Events: 8917 mean_circ: 0.946507



End this measurment..



Stopping time: 2.54265e+009
****************************************
Measurement finished

Mean Circularitiy: 0.946506
Mean Area: 600.77
Mean FL-1 intensity: 3309.17
Mean FL-2 intensity: 3123.93
Mean FL-3 intensity: 0

frames processed: 146812
Total Time: 74917.9ms
Number of Runs (new bg img) 0
Frames collected: 149680
Skipped frames: 67807
Rescued Frames: 5217124
Samples DAQ: 37420059
Samples written: 74840036
Samples per Channel: 37420018
FPS: 1997.92

Samples per second: 499480
Expected samples/s: 500000

Events: 8962
Events per second: 119.624
jumped back (PMT): 3608
#Frames read, frequency
# 0 frames :243898
# 1 frames :42463
# 2 frames :3335
# 3 frames :950
# 4 frames :690
# 5 frames :344
# 6 frames :1109
# 7 frames :1272
# 8 frames :1067
# 9 frames :716
# 10 frames :518
# 11 frames :363
# 12 frames :289
# 13 frames :224
# 14 frames :203
# 15 frames :238
# 16 frames :205
# 17 frames :167
# 18 frames :144
# 19 frames :108
# 20 frames :99
# 21 frames :99
# 22 frames :75
# 23 frames :74
# 24 frames :68
# 25 frames :51
# 26 frames :47
# 27 frames :44
# 28 frames :37
# 29 frames :36
# 30 frames :41
# 31 frames :30
# 32 frames :40
# 33 frames :20
# 34 frames :30
# 35 frames :24
# 36 frames :18
# 37 frames :18
# 38 frames :11
# 39 frames :14
# 40 frames :12
# 41 frames :9
# 42 frames :11
# 43 frames :7
# 44 frames :10
# 45 frames :10
# 46 frames :6
# 47 frames :6
# 48 frames :8
# 49 frames :7
# 50 frames :5
# 51 frames :3
# 52 frames :5
# 53 frames :5
# 54 frames :7
# 55 frames :3
# 56 frames :1
# 59 frames :1
# 60 frames :3
# 62 frames :4
# 63 frames :3
# 64 frames :3
# 67 frames :4
# 68 frames :1
# 72 frames :1
# 76 frames :1
# 77 frames :1
# 78 frames :1
# 84 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7318
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0