Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.28.59 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "26eefb5728b7a448c03d23ed5c2009b3",
"name": "M1_20170613_1842_dsRNA_Y2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:29:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9338 (max 9339) in '9338'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7071
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54275e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 80 mean_circ: 0.929414
Ran for 3050 frames Cell Events: 224 mean_circ: 0.933726
Ran for 3550 frames Cell Events: 244 mean_circ: 0.935264
Ran for 5550 frames Cell Events: 379 mean_circ: 0.935814
Ran for 10050 frames Cell Events: 630 mean_circ: 0.936585
Ran for 11550 frames Cell Events: 731 mean_circ: 0.936319
Ran for 12050 frames Cell Events: 757 mean_circ: 0.936401
Ran for 14050 frames Cell Events: 900 mean_circ: 0.936756
Ran for 14550 frames Cell Events: 937 mean_circ: 0.936401
Ran for 16550 frames Cell Events: 1066 mean_circ: 0.936871
Ran for 17050 frames Cell Events: 1090 mean_circ: 0.936931
Ran for 18550 frames Cell Events: 1177 mean_circ: 0.937355
Ran for 20050 frames Cell Events: 1295 mean_circ: 0.937806
Ran for 24050 frames Cell Events: 1592 mean_circ: 0.938441
Ran for 26050 frames Cell Events: 1746 mean_circ: 0.93784
Ran for 27550 frames Cell Events: 1828 mean_circ: 0.937817
Ran for 30050 frames Cell Events: 2000 mean_circ: 0.937709
Ran for 32050 frames Cell Events: 2133 mean_circ: 0.937693
Ran for 32550 frames Cell Events: 2160 mean_circ: 0.937496
Ran for 36050 frames Cell Events: 2423 mean_circ: 0.937065
Ran for 38050 frames Cell Events: 2561 mean_circ: 0.937252
Ran for 40050 frames Cell Events: 2713 mean_circ: 0.937172
Ran for 40550 frames Cell Events: 2739 mean_circ: 0.937095
Ran for 42050 frames Cell Events: 2826 mean_circ: 0.937015
Ran for 44550 frames Cell Events: 2997 mean_circ: 0.937231
Ran for 45050 frames Cell Events: 3026 mean_circ: 0.937279
Ran for 45550 frames Cell Events: 3058 mean_circ: 0.937224
Ran for 46050 frames Cell Events: 3075 mean_circ: 0.937076
Ran for 48050 frames Cell Events: 3218 mean_circ: 0.937244
Ran for 48550 frames Cell Events: 3246 mean_circ: 0.937259
Ran for 49050 frames Cell Events: 3274 mean_circ: 0.937363
Ran for 51550 frames Cell Events: 3470 mean_circ: 0.937416
Ran for 52050 frames Cell Events: 3510 mean_circ: 0.937416
Ran for 53550 frames Cell Events: 3619 mean_circ: 0.937233
Ran for 56050 frames Cell Events: 3810 mean_circ: 0.93725
Ran for 57050 frames Cell Events: 3856 mean_circ: 0.937314
Ran for 58550 frames Cell Events: 3958 mean_circ: 0.93736
Ran for 60550 frames Cell Events: 4086 mean_circ: 0.937154
Ran for 61550 frames Cell Events: 4161 mean_circ: 0.93724
Ran for 63050 frames Cell Events: 4230 mean_circ: 0.937254
Ran for 65550 frames Cell Events: 4421 mean_circ: 0.937231
Ran for 68550 frames Cell Events: 4650 mean_circ: 0.937215
Ran for 69050 frames Cell Events: 4679 mean_circ: 0.937229
Ran for 70050 frames Cell Events: 4740 mean_circ: 0.937282
Ran for 71050 frames Cell Events: 4797 mean_circ: 0.937305
Ran for 74050 frames Cell Events: 5027 mean_circ: 0.937423
Ran for 75550 frames Cell Events: 5111 mean_circ: 0.937457
Ran for 78050 frames Cell Events: 5265 mean_circ: 0.937546
Ran for 78550 frames Cell Events: 5294 mean_circ: 0.937611
Ran for 80550 frames Cell Events: 5429 mean_circ: 0.937549
Ran for 90550 frames Cell Events: 6156 mean_circ: 0.93758
Ran for 92550 frames Cell Events: 6265 mean_circ: 0.937533
Ran for 94050 frames Cell Events: 6368 mean_circ: 0.937639
Ran for 95050 frames Cell Events: 6429 mean_circ: 0.937714
Ran for 96050 frames Cell Events: 6491 mean_circ: 0.937766
Ran for 96550 frames Cell Events: 6515 mean_circ: 0.937797
Ran for 97050 frames Cell Events: 6556 mean_circ: 0.937772
Ran for 100550 frames Cell Events: 6838 mean_circ: 0.937838
Ran for 102550 frames Cell Events: 6971 mean_circ: 0.937739
Ran for 104550 frames Cell Events: 7112 mean_circ: 0.937691
Ran for 105050 frames Cell Events: 7142 mean_circ: 0.937685
Ran for 105550 frames Cell Events: 7166 mean_circ: 0.937652
Ran for 109050 frames Cell Events: 7420 mean_circ: 0.937573
Ran for 110550 frames Cell Events: 7547 mean_circ: 0.937655
Ran for 112050 frames Cell Events: 7665 mean_circ: 0.9376
Ran for 114050 frames Cell Events: 7819 mean_circ: 0.937572
Ran for 117050 frames Cell Events: 8019 mean_circ: 0.93745
Ran for 127050 frames Cell Events: 8725 mean_circ: 0.93727
Ran for 128050 frames Cell Events: 8805 mean_circ: 0.937257
Ran for 129050 frames Cell Events: 8860 mean_circ: 0.937212
Ran for 132050 frames Cell Events: 9053 mean_circ: 0.93725
Ran for 134050 frames Cell Events: 9205 mean_circ: 0.937237



End this measurment..



Stopping time: 2.54282e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937162
Mean Area: 591.822
Mean FL-1 intensity: 3125.32
Mean FL-2 intensity: 3380.22
Mean FL-3 intensity: 0

frames processed: 130648
Total Time: 67910ms
Number of Runs (new bg img) 0
Frames collected: 135651
Skipped frames: 72810
Rescued Frames: 5306006
Samples DAQ: 33919589
Samples written: 67825528
Samples per Channel: 33912764
FPS: 1997.51

Samples per second: 499378
Expected samples/s: 500000

Events: 9339
Events per second: 137.52
jumped back (PMT): 40
#Frames read, frequency
# 0 frames :218232
# 1 frames :30641
# 2 frames :2273
# 3 frames :823
# 4 frames :569
# 5 frames :301
# 6 frames :1338
# 7 frames :942
# 8 frames :922
# 9 frames :576
# 10 frames :403
# 11 frames :295
# 12 frames :234
# 13 frames :233
# 14 frames :241
# 15 frames :249
# 16 frames :200
# 17 frames :168
# 18 frames :149
# 19 frames :116
# 20 frames :97
# 21 frames :109
# 22 frames :91
# 23 frames :93
# 24 frames :67
# 25 frames :53
# 26 frames :48
# 27 frames :57
# 28 frames :49
# 29 frames :41
# 30 frames :45
# 31 frames :43
# 32 frames :28
# 33 frames :31
# 34 frames :30
# 35 frames :20
# 36 frames :24
# 37 frames :21
# 38 frames :22
# 39 frames :16
# 40 frames :11
# 41 frames :18
# 42 frames :15
# 43 frames :8
# 44 frames :13
# 45 frames :6
# 46 frames :6
# 47 frames :7
# 48 frames :4
# 49 frames :12
# 50 frames :7
# 51 frames :5
# 52 frames :6
# 53 frames :4
# 54 frames :3
# 55 frames :2
# 56 frames :4
# 57 frames :2
# 58 frames :5
# 59 frames :4
# 60 frames :2
# 61 frames :4
# 62 frames :2
# 63 frames :5
# 64 frames :3
# 65 frames :4
# 66 frames :1
# 67 frames :6
# 68 frames :1
# 69 frames :1
# 72 frames :1
# 77 frames :2
# 78 frames :1
# 79 frames :2
# 80 frames :1
# 82 frames :1
# 98 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7071
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0