Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.30.27 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7499de521501cf11eb0665429745bb84",
"name": "M1_20170613_1900_dsRNA_X2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:30:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8422 (max 8423) in '8422'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5933
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54386e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 40 mean_circ: 0.954928
Ran for 2550 frames Cell Events: 116 mean_circ: 0.954252
Ran for 3050 frames Cell Events: 136 mean_circ: 0.955273
Ran for 3550 frames Cell Events: 164 mean_circ: 0.956827
Ran for 4050 frames Cell Events: 180 mean_circ: 0.956503
Ran for 5050 frames Cell Events: 224 mean_circ: 0.954881
Ran for 5550 frames Cell Events: 259 mean_circ: 0.954881
Ran for 6050 frames Cell Events: 289 mean_circ: 0.954098
Ran for 6550 frames Cell Events: 325 mean_circ: 0.953607
Ran for 8050 frames Cell Events: 412 mean_circ: 0.954537
Ran for 8550 frames Cell Events: 433 mean_circ: 0.955215
Ran for 9050 frames Cell Events: 447 mean_circ: 0.95499
Ran for 9550 frames Cell Events: 464 mean_circ: 0.954999
Ran for 11050 frames Cell Events: 538 mean_circ: 0.954948
Ran for 12550 frames Cell Events: 623 mean_circ: 0.953799
Ran for 13050 frames Cell Events: 643 mean_circ: 0.954158
Ran for 14550 frames Cell Events: 719 mean_circ: 0.95406
Ran for 15050 frames Cell Events: 746 mean_circ: 0.953966
Ran for 16050 frames Cell Events: 808 mean_circ: 0.953209
Ran for 17050 frames Cell Events: 848 mean_circ: 0.952836
Ran for 17550 frames Cell Events: 879 mean_circ: 0.952623
Ran for 18050 frames Cell Events: 908 mean_circ: 0.952604
Ran for 18550 frames Cell Events: 932 mean_circ: 0.952798
Ran for 19050 frames Cell Events: 956 mean_circ: 0.952863
Ran for 20050 frames Cell Events: 1028 mean_circ: 0.952848
Ran for 21050 frames Cell Events: 1080 mean_circ: 0.952647
Ran for 21550 frames Cell Events: 1114 mean_circ: 0.952645
Ran for 22050 frames Cell Events: 1142 mean_circ: 0.952814
Ran for 22550 frames Cell Events: 1165 mean_circ: 0.952878
Ran for 24050 frames Cell Events: 1233 mean_circ: 0.95299
Ran for 26050 frames Cell Events: 1338 mean_circ: 0.953171
Ran for 26550 frames Cell Events: 1361 mean_circ: 0.953354
Ran for 27050 frames Cell Events: 1386 mean_circ: 0.953327
Ran for 28050 frames Cell Events: 1425 mean_circ: 0.953417
Ran for 28550 frames Cell Events: 1461 mean_circ: 0.953497
Ran for 29050 frames Cell Events: 1495 mean_circ: 0.953521
Ran for 29550 frames Cell Events: 1520 mean_circ: 0.953289
Ran for 30050 frames Cell Events: 1545 mean_circ: 0.953301
Ran for 30550 frames Cell Events: 1558 mean_circ: 0.953237
Ran for 31050 frames Cell Events: 1596 mean_circ: 0.953335
Ran for 32050 frames Cell Events: 1645 mean_circ: 0.953281
Ran for 33550 frames Cell Events: 1737 mean_circ: 0.953477
Ran for 34050 frames Cell Events: 1764 mean_circ: 0.953511
Ran for 35550 frames Cell Events: 1854 mean_circ: 0.953423
Ran for 36050 frames Cell Events: 1882 mean_circ: 0.953582
Ran for 39050 frames Cell Events: 2091 mean_circ: 0.953076
Ran for 39550 frames Cell Events: 2113 mean_circ: 0.95319
Ran for 40050 frames Cell Events: 2146 mean_circ: 0.95323
Ran for 40550 frames Cell Events: 2161 mean_circ: 0.953234
Ran for 41050 frames Cell Events: 2182 mean_circ: 0.953239
Ran for 42550 frames Cell Events: 2264 mean_circ: 0.953212
Ran for 43550 frames Cell Events: 2306 mean_circ: 0.953319
Ran for 44050 frames Cell Events: 2338 mean_circ: 0.953369
Ran for 45050 frames Cell Events: 2401 mean_circ: 0.952957
Ran for 46050 frames Cell Events: 2452 mean_circ: 0.95299
Ran for 47050 frames Cell Events: 2498 mean_circ: 0.95291
Ran for 48550 frames Cell Events: 2579 mean_circ: 0.952921
Ran for 49550 frames Cell Events: 2659 mean_circ: 0.952723
Ran for 50550 frames Cell Events: 2711 mean_circ: 0.952593
Ran for 51050 frames Cell Events: 2729 mean_circ: 0.952569
Ran for 51550 frames Cell Events: 2752 mean_circ: 0.952593
Ran for 52550 frames Cell Events: 2812 mean_circ: 0.95244
Ran for 53550 frames Cell Events: 2864 mean_circ: 0.952395
Ran for 54050 frames Cell Events: 2890 mean_circ: 0.952335
Ran for 55050 frames Cell Events: 2953 mean_circ: 0.95228
Ran for 55550 frames Cell Events: 2974 mean_circ: 0.952318
Ran for 56050 frames Cell Events: 3002 mean_circ: 0.9523
Ran for 57550 frames Cell Events: 3077 mean_circ: 0.952071
Ran for 58050 frames Cell Events: 3089 mean_circ: 0.952076
Ran for 58550 frames Cell Events: 3112 mean_circ: 0.952072
Ran for 59050 frames Cell Events: 3133 mean_circ: 0.952141
Ran for 59550 frames Cell Events: 3163 mean_circ: 0.952163
Ran for 60550 frames Cell Events: 3214 mean_circ: 0.952259
Ran for 61050 frames Cell Events: 3235 mean_circ: 0.952266
Ran for 62550 frames Cell Events: 3319 mean_circ: 0.952469
Ran for 64550 frames Cell Events: 3448 mean_circ: 0.95238
Ran for 66050 frames Cell Events: 3540 mean_circ: 0.952588
Ran for 66550 frames Cell Events: 3572 mean_circ: 0.952578
Ran for 67050 frames Cell Events: 3589 mean_circ: 0.952547
Ran for 69050 frames Cell Events: 3709 mean_circ: 0.952555
Ran for 69550 frames Cell Events: 3744 mean_circ: 0.952637
Ran for 70550 frames Cell Events: 3798 mean_circ: 0.952713
Ran for 72550 frames Cell Events: 3917 mean_circ: 0.952602
Ran for 74550 frames Cell Events: 4031 mean_circ: 0.952727
Ran for 75050 frames Cell Events: 4057 mean_circ: 0.952688
Ran for 75550 frames Cell Events: 4083 mean_circ: 0.952707
Ran for 76050 frames Cell Events: 4104 mean_circ: 0.952783
Ran for 77050 frames Cell Events: 4155 mean_circ: 0.95277
Ran for 78550 frames Cell Events: 4249 mean_circ: 0.952679
Ran for 82050 frames Cell Events: 4507 mean_circ: 0.952778
Ran for 82550 frames Cell Events: 4530 mean_circ: 0.952736
Ran for 84550 frames Cell Events: 4651 mean_circ: 0.952771
Ran for 85550 frames Cell Events: 4714 mean_circ: 0.952798
Ran for 86050 frames Cell Events: 4739 mean_circ: 0.952807
Ran for 87050 frames Cell Events: 4805 mean_circ: 0.95271
Ran for 88550 frames Cell Events: 4893 mean_circ: 0.952745
Ran for 89550 frames Cell Events: 4955 mean_circ: 0.95263
Ran for 91550 frames Cell Events: 5072 mean_circ: 0.952721
Ran for 92550 frames Cell Events: 5119 mean_circ: 0.952741
Ran for 93550 frames Cell Events: 5175 mean_circ: 0.952786
Ran for 94050 frames Cell Events: 5191 mean_circ: 0.952786
Ran for 94550 frames Cell Events: 5213 mean_circ: 0.952761
Ran for 96050 frames Cell Events: 5314 mean_circ: 0.95277
Ran for 96550 frames Cell Events: 5340 mean_circ: 0.952807
Ran for 97050 frames Cell Events: 5372 mean_circ: 0.952814
Ran for 98050 frames Cell Events: 5431 mean_circ: 0.952813
Ran for 99550 frames Cell Events: 5492 mean_circ: 0.952757
Ran for 100550 frames Cell Events: 5562 mean_circ: 0.95281
Ran for 102050 frames Cell Events: 5659 mean_circ: 0.95273
Ran for 103050 frames Cell Events: 5698 mean_circ: 0.952694
Ran for 105050 frames Cell Events: 5812 mean_circ: 0.952736
Ran for 105550 frames Cell Events: 5842 mean_circ: 0.952739
Ran for 106050 frames Cell Events: 5870 mean_circ: 0.952654
Ran for 107050 frames Cell Events: 5951 mean_circ: 0.952678
Ran for 107550 frames Cell Events: 5984 mean_circ: 0.952678
Ran for 108550 frames Cell Events: 6036 mean_circ: 0.952652
Ran for 109050 frames Cell Events: 6064 mean_circ: 0.952688
Ran for 109550 frames Cell Events: 6091 mean_circ: 0.952696
Ran for 110050 frames Cell Events: 6117 mean_circ: 0.952687
Ran for 112050 frames Cell Events: 6224 mean_circ: 0.952659
Ran for 113050 frames Cell Events: 6279 mean_circ: 0.95258
Ran for 114550 frames Cell Events: 6362 mean_circ: 0.952582
Ran for 116050 frames Cell Events: 6464 mean_circ: 0.952502
Ran for 117050 frames Cell Events: 6527 mean_circ: 0.95243
Ran for 118550 frames Cell Events: 6608 mean_circ: 0.952384
Ran for 120550 frames Cell Events: 6733 mean_circ: 0.952358
Ran for 122050 frames Cell Events: 6829 mean_circ: 0.952317
Ran for 123050 frames Cell Events: 6883 mean_circ: 0.952267
Ran for 123550 frames Cell Events: 6914 mean_circ: 0.952267
Ran for 124550 frames Cell Events: 6966 mean_circ: 0.952214
Ran for 126050 frames Cell Events: 7073 mean_circ: 0.952218
Ran for 127550 frames Cell Events: 7172 mean_circ: 0.952254
Ran for 130550 frames Cell Events: 7377 mean_circ: 0.95215
Ran for 131050 frames Cell Events: 7407 mean_circ: 0.952129
Ran for 131550 frames Cell Events: 7441 mean_circ: 0.952145
Ran for 132550 frames Cell Events: 7502 mean_circ: 0.95219
Ran for 133050 frames Cell Events: 7528 mean_circ: 0.9522
Ran for 133550 frames Cell Events: 7549 mean_circ: 0.952149
Ran for 134050 frames Cell Events: 7574 mean_circ: 0.952138
Ran for 134550 frames Cell Events: 7619 mean_circ: 0.952074
Ran for 135050 frames Cell Events: 7644 mean_circ: 0.952084
Ran for 135550 frames Cell Events: 7676 mean_circ: 0.95208
Ran for 136050 frames Cell Events: 7701 mean_circ: 0.952045
Ran for 136550 frames Cell Events: 7729 mean_circ: 0.952062
Ran for 137050 frames Cell Events: 7739 mean_circ: 0.952082
Ran for 137550 frames Cell Events: 7769 mean_circ: 0.952072
Ran for 138550 frames Cell Events: 7821 mean_circ: 0.952076
Ran for 139550 frames Cell Events: 7884 mean_circ: 0.952054
Ran for 140550 frames Cell Events: 7946 mean_circ: 0.952003
Ran for 141050 frames Cell Events: 7966 mean_circ: 0.951991
Ran for 141550 frames Cell Events: 7990 mean_circ: 0.952015
Ran for 143050 frames Cell Events: 8105 mean_circ: 0.952082
Ran for 146050 frames Cell Events: 8268 mean_circ: 0.952105
Ran for 146550 frames Cell Events: 8286 mean_circ: 0.952078



End this measurment..



Stopping time: 2.54393e+009
****************************************
Measurement finished

Mean Circularitiy: 0.952075
Mean Area: 588.452
Mean FL-1 intensity: 3126.43
Mean FL-2 intensity: 3055.46
Mean FL-3 intensity: 0

frames processed: 148149
Total Time: 74510.2ms
Number of Runs (new bg img) 0
Frames collected: 148855
Skipped frames: 74958
Rescued Frames: 5571802
Samples DAQ: 37213826
Samples written: 74427328
Samples per Channel: 37213664
FPS: 1997.78

Samples per second: 499444
Expected samples/s: 500000

Events: 8423
Events per second: 113.045
jumped back (PMT): 3974
#Frames read, frequency
# 0 frames :230153
# 1 frames :54276
# 2 frames :3464
# 3 frames :1098
# 4 frames :635
# 5 frames :329
# 6 frames :1916
# 7 frames :1201
# 8 frames :1215
# 9 frames :606
# 10 frames :395
# 11 frames :282
# 12 frames :282
# 13 frames :219
# 14 frames :291
# 15 frames :208
# 16 frames :198
# 17 frames :143
# 18 frames :120
# 19 frames :80
# 20 frames :81
# 21 frames :78
# 22 frames :60
# 23 frames :55
# 24 frames :45
# 25 frames :43
# 26 frames :20
# 27 frames :26
# 28 frames :12
# 29 frames :26
# 30 frames :17
# 31 frames :17
# 32 frames :14
# 33 frames :11
# 34 frames :8
# 35 frames :7
# 36 frames :5
# 37 frames :6
# 38 frames :5
# 39 frames :5
# 40 frames :3
# 41 frames :5
# 42 frames :4
# 43 frames :7
# 44 frames :1
# 46 frames :2
# 47 frames :1
# 48 frames :3
# 50 frames :1
# 51 frames :1
# 53 frames :1
# 54 frames :2
# 55 frames :1
# 56 frames :1
# 58 frames :1
# 59 frames :1
# 62 frames :1
# 95 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5933
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0