Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.32.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a797abc6dc3b840d97647b648181e849",
"name": "M1_20170613_1926_dsRNA_Y1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:33:15"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8633 (max 8634) in '8633'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7008
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54539e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 53 mean_circ: 0.923203
Ran for 1550 frames Cell Events: 81 mean_circ: 0.922968
Ran for 2550 frames Cell Events: 138 mean_circ: 0.925302
Ran for 3050 frames Cell Events: 171 mean_circ: 0.925293
Ran for 3550 frames Cell Events: 191 mean_circ: 0.925481
Ran for 6050 frames Cell Events: 285 mean_circ: 0.928763
Ran for 6550 frames Cell Events: 312 mean_circ: 0.929273
Ran for 7550 frames Cell Events: 362 mean_circ: 0.929116
Ran for 8050 frames Cell Events: 387 mean_circ: 0.92877
Ran for 9050 frames Cell Events: 434 mean_circ: 0.927249
Ran for 9550 frames Cell Events: 464 mean_circ: 0.927236
Ran for 10050 frames Cell Events: 493 mean_circ: 0.927026
Ran for 10550 frames Cell Events: 517 mean_circ: 0.926763
Ran for 11050 frames Cell Events: 540 mean_circ: 0.926501
Ran for 11550 frames Cell Events: 572 mean_circ: 0.925961
Ran for 12050 frames Cell Events: 603 mean_circ: 0.926182
Ran for 12550 frames Cell Events: 638 mean_circ: 0.92619
Ran for 13050 frames Cell Events: 668 mean_circ: 0.927242
Ran for 13550 frames Cell Events: 694 mean_circ: 0.926846
Ran for 15050 frames Cell Events: 819 mean_circ: 0.927035
Ran for 15550 frames Cell Events: 841 mean_circ: 0.926572
Ran for 16550 frames Cell Events: 887 mean_circ: 0.926369
Ran for 17050 frames Cell Events: 910 mean_circ: 0.926571
Ran for 17550 frames Cell Events: 928 mean_circ: 0.926667
Ran for 18050 frames Cell Events: 967 mean_circ: 0.926839
Ran for 19050 frames Cell Events: 1022 mean_circ: 0.926681
Ran for 21050 frames Cell Events: 1138 mean_circ: 0.926959
Ran for 21550 frames Cell Events: 1164 mean_circ: 0.926868
Ran for 22050 frames Cell Events: 1190 mean_circ: 0.92692
Ran for 22550 frames Cell Events: 1212 mean_circ: 0.926497
Ran for 23050 frames Cell Events: 1232 mean_circ: 0.92666
Ran for 23550 frames Cell Events: 1262 mean_circ: 0.926561
Ran for 24050 frames Cell Events: 1296 mean_circ: 0.926269
Ran for 25050 frames Cell Events: 1342 mean_circ: 0.925868
Ran for 26550 frames Cell Events: 1445 mean_circ: 0.925761
Ran for 27050 frames Cell Events: 1479 mean_circ: 0.925468
Ran for 27550 frames Cell Events: 1500 mean_circ: 0.925668
Ran for 28050 frames Cell Events: 1519 mean_circ: 0.925636
Ran for 28550 frames Cell Events: 1534 mean_circ: 0.925622
Ran for 30050 frames Cell Events: 1622 mean_circ: 0.926005
Ran for 32550 frames Cell Events: 1753 mean_circ: 0.926153
Ran for 33550 frames Cell Events: 1819 mean_circ: 0.926129
Ran for 34050 frames Cell Events: 1849 mean_circ: 0.926264
Ran for 34550 frames Cell Events: 1876 mean_circ: 0.926404
Ran for 36050 frames Cell Events: 1986 mean_circ: 0.926265
Ran for 36550 frames Cell Events: 2009 mean_circ: 0.92642
Ran for 37550 frames Cell Events: 2081 mean_circ: 0.926593
Ran for 38550 frames Cell Events: 2143 mean_circ: 0.926713
Ran for 39050 frames Cell Events: 2165 mean_circ: 0.926775
Ran for 40050 frames Cell Events: 2206 mean_circ: 0.926788
Ran for 40550 frames Cell Events: 2229 mean_circ: 0.926648
Ran for 41050 frames Cell Events: 2251 mean_circ: 0.926722
Ran for 41550 frames Cell Events: 2285 mean_circ: 0.926758
Ran for 42050 frames Cell Events: 2305 mean_circ: 0.926933
Ran for 42550 frames Cell Events: 2325 mean_circ: 0.926931
Ran for 43050 frames Cell Events: 2345 mean_circ: 0.926935
Ran for 43550 frames Cell Events: 2363 mean_circ: 0.926814
Ran for 44050 frames Cell Events: 2384 mean_circ: 0.926693
Ran for 44550 frames Cell Events: 2407 mean_circ: 0.926802
Ran for 45050 frames Cell Events: 2440 mean_circ: 0.926652
Ran for 46050 frames Cell Events: 2496 mean_circ: 0.926703
Ran for 47550 frames Cell Events: 2564 mean_circ: 0.926913
Ran for 48550 frames Cell Events: 2616 mean_circ: 0.92664
Ran for 49550 frames Cell Events: 2658 mean_circ: 0.926621
Ran for 50050 frames Cell Events: 2677 mean_circ: 0.926786
Ran for 50550 frames Cell Events: 2698 mean_circ: 0.926898
Ran for 51550 frames Cell Events: 2734 mean_circ: 0.92697
Ran for 52050 frames Cell Events: 2765 mean_circ: 0.927066
Ran for 52550 frames Cell Events: 2797 mean_circ: 0.927067
Ran for 54050 frames Cell Events: 2884 mean_circ: 0.926847
Ran for 55050 frames Cell Events: 2958 mean_circ: 0.926857
Ran for 55550 frames Cell Events: 2996 mean_circ: 0.926745
Ran for 56050 frames Cell Events: 3017 mean_circ: 0.92673
Ran for 57050 frames Cell Events: 3056 mean_circ: 0.926854
Ran for 57550 frames Cell Events: 3073 mean_circ: 0.926931
Ran for 58050 frames Cell Events: 3092 mean_circ: 0.926953
Ran for 59550 frames Cell Events: 3167 mean_circ: 0.926958
Ran for 60050 frames Cell Events: 3198 mean_circ: 0.926961
Ran for 60550 frames Cell Events: 3224 mean_circ: 0.92707
Ran for 61550 frames Cell Events: 3269 mean_circ: 0.927123
Ran for 63050 frames Cell Events: 3356 mean_circ: 0.927014
Ran for 63550 frames Cell Events: 3389 mean_circ: 0.927021
Ran for 64050 frames Cell Events: 3412 mean_circ: 0.927012
Ran for 64550 frames Cell Events: 3437 mean_circ: 0.927015
Ran for 65550 frames Cell Events: 3482 mean_circ: 0.926967
Ran for 66050 frames Cell Events: 3517 mean_circ: 0.926967
Ran for 66550 frames Cell Events: 3549 mean_circ: 0.927131
Ran for 67550 frames Cell Events: 3598 mean_circ: 0.927434
Ran for 68050 frames Cell Events: 3614 mean_circ: 0.927441
Ran for 68550 frames Cell Events: 3636 mean_circ: 0.92734
Ran for 69550 frames Cell Events: 3676 mean_circ: 0.927427
Ran for 70050 frames Cell Events: 3712 mean_circ: 0.927564
Ran for 70550 frames Cell Events: 3737 mean_circ: 0.927526
Ran for 71050 frames Cell Events: 3764 mean_circ: 0.927489
Ran for 72050 frames Cell Events: 3820 mean_circ: 0.92764
Ran for 72550 frames Cell Events: 3843 mean_circ: 0.927634
Ran for 73050 frames Cell Events: 3865 mean_circ: 0.927571
Ran for 73550 frames Cell Events: 3881 mean_circ: 0.927577
Ran for 74050 frames Cell Events: 3909 mean_circ: 0.927575
Ran for 75050 frames Cell Events: 3955 mean_circ: 0.927401
Ran for 76050 frames Cell Events: 4018 mean_circ: 0.927491
Ran for 76550 frames Cell Events: 4044 mean_circ: 0.927398
Ran for 77550 frames Cell Events: 4100 mean_circ: 0.927308
Ran for 79550 frames Cell Events: 4206 mean_circ: 0.927121
Ran for 81550 frames Cell Events: 4353 mean_circ: 0.927222
Ran for 82550 frames Cell Events: 4425 mean_circ: 0.927203
Ran for 83050 frames Cell Events: 4457 mean_circ: 0.927174
Ran for 85050 frames Cell Events: 4615 mean_circ: 0.927175
Ran for 85550 frames Cell Events: 4654 mean_circ: 0.927198
Ran for 86050 frames Cell Events: 4677 mean_circ: 0.927246
Ran for 86550 frames Cell Events: 4708 mean_circ: 0.927224
Ran for 88050 frames Cell Events: 4772 mean_circ: 0.927042
Ran for 88550 frames Cell Events: 4796 mean_circ: 0.927
Ran for 89050 frames Cell Events: 4818 mean_circ: 0.927041
Ran for 90050 frames Cell Events: 4864 mean_circ: 0.927043
Ran for 90550 frames Cell Events: 4892 mean_circ: 0.926989
Ran for 91550 frames Cell Events: 4937 mean_circ: 0.926942
Ran for 92050 frames Cell Events: 4963 mean_circ: 0.926951
Ran for 93550 frames Cell Events: 5056 mean_circ: 0.927065
Ran for 94550 frames Cell Events: 5106 mean_circ: 0.927081
Ran for 95550 frames Cell Events: 5161 mean_circ: 0.927067
Ran for 96050 frames Cell Events: 5196 mean_circ: 0.927057
Ran for 96550 frames Cell Events: 5217 mean_circ: 0.927122
Ran for 99050 frames Cell Events: 5343 mean_circ: 0.927187
Ran for 99550 frames Cell Events: 5363 mean_circ: 0.927221
Ran for 100050 frames Cell Events: 5387 mean_circ: 0.92721
Ran for 101050 frames Cell Events: 5451 mean_circ: 0.927168
Ran for 101550 frames Cell Events: 5486 mean_circ: 0.927179
Ran for 102050 frames Cell Events: 5521 mean_circ: 0.9271
Ran for 103050 frames Cell Events: 5582 mean_circ: 0.927199
Ran for 104050 frames Cell Events: 5628 mean_circ: 0.927204
Ran for 105550 frames Cell Events: 5671 mean_circ: 0.927251
Ran for 106050 frames Cell Events: 5706 mean_circ: 0.927171
Ran for 106550 frames Cell Events: 5731 mean_circ: 0.927186
Ran for 107550 frames Cell Events: 5789 mean_circ: 0.927173
Ran for 108550 frames Cell Events: 5848 mean_circ: 0.927203
Ran for 109050 frames Cell Events: 5869 mean_circ: 0.927278
Ran for 110050 frames Cell Events: 5909 mean_circ: 0.927079
Ran for 111550 frames Cell Events: 6003 mean_circ: 0.927056
Ran for 112050 frames Cell Events: 6033 mean_circ: 0.927024
Ran for 113050 frames Cell Events: 6105 mean_circ: 0.926804
Ran for 114050 frames Cell Events: 6157 mean_circ: 0.926845
Ran for 116050 frames Cell Events: 6302 mean_circ: 0.926941
Ran for 117550 frames Cell Events: 6391 mean_circ: 0.926983
Ran for 119050 frames Cell Events: 6498 mean_circ: 0.92697
Ran for 120050 frames Cell Events: 6558 mean_circ: 0.927044
Ran for 121050 frames Cell Events: 6611 mean_circ: 0.927045
Ran for 122050 frames Cell Events: 6649 mean_circ: 0.927072
Ran for 123050 frames Cell Events: 6695 mean_circ: 0.92708
Ran for 123550 frames Cell Events: 6723 mean_circ: 0.927127
Ran for 124550 frames Cell Events: 6772 mean_circ: 0.927153
Ran for 125050 frames Cell Events: 6808 mean_circ: 0.92721
Ran for 125550 frames Cell Events: 6827 mean_circ: 0.92719
Ran for 128050 frames Cell Events: 6994 mean_circ: 0.927218
Ran for 129550 frames Cell Events: 7099 mean_circ: 0.927302
Ran for 131550 frames Cell Events: 7208 mean_circ: 0.927292
Ran for 132050 frames Cell Events: 7230 mean_circ: 0.927265
Ran for 132550 frames Cell Events: 7250 mean_circ: 0.92727
Ran for 133550 frames Cell Events: 7303 mean_circ: 0.927255
Ran for 135050 frames Cell Events: 7399 mean_circ: 0.92723
Ran for 137050 frames Cell Events: 7537 mean_circ: 0.927114
Ran for 137550 frames Cell Events: 7563 mean_circ: 0.927105
Ran for 138050 frames Cell Events: 7585 mean_circ: 0.927096
Ran for 138550 frames Cell Events: 7608 mean_circ: 0.927085
Ran for 139050 frames Cell Events: 7634 mean_circ: 0.927093
Ran for 143550 frames Cell Events: 7912 mean_circ: 0.927077
Ran for 145050 frames Cell Events: 8011 mean_circ: 0.927042
Ran for 145550 frames Cell Events: 8030 mean_circ: 0.927107
Ran for 146050 frames Cell Events: 8057 mean_circ: 0.927104
Ran for 147050 frames Cell Events: 8099 mean_circ: 0.92711
Ran for 148550 frames Cell Events: 8172 mean_circ: 0.927077
Ran for 149050 frames Cell Events: 8195 mean_circ: 0.927085
Ran for 150050 frames Cell Events: 8261 mean_circ: 0.927091
Ran for 151050 frames Cell Events: 8321 mean_circ: 0.927109
Ran for 152550 frames Cell Events: 8405 mean_circ: 0.927023
Ran for 153050 frames Cell Events: 8424 mean_circ: 0.92701
Ran for 154050 frames Cell Events: 8482 mean_circ: 0.927006
Ran for 155050 frames Cell Events: 8524 mean_circ: 0.926976
Ran for 156050 frames Cell Events: 8566 mean_circ: 0.926998
Ran for 156550 frames Cell Events: 8604 mean_circ: 0.927055
Ran for 157050 frames Cell Events: 8620 mean_circ: 0.927057



End this measurment..



Stopping time: 2.54546e+009
****************************************
Measurement finished

Mean Circularitiy: 0.92702
Mean Area: 645.335
Mean FL-1 intensity: 3005.63
Mean FL-2 intensity: 3632.49
Mean FL-3 intensity: 0

frames processed: 155878
Total Time: 78753.2ms
Number of Runs (new bg img) 0
Frames collected: 157352
Skipped frames: 80154
Rescued Frames: 6007487
Samples DAQ: 39337920
Samples written: 78675722
Samples per Channel: 39337861
FPS: 1998.04

Samples per second: 499508
Expected samples/s: 500000

Events: 8634
Events per second: 109.634
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :235882
# 1 frames :65024
# 2 frames :3240
# 3 frames :1328
# 4 frames :611
# 5 frames :275
# 6 frames :2962
# 7 frames :1274
# 8 frames :1113
# 9 frames :605
# 10 frames :417
# 11 frames :293
# 12 frames :257
# 13 frames :206
# 14 frames :210
# 15 frames :165
# 16 frames :127
# 17 frames :104
# 18 frames :85
# 19 frames :45
# 20 frames :48
# 21 frames :47
# 22 frames :51
# 23 frames :32
# 24 frames :23
# 25 frames :28
# 26 frames :19
# 27 frames :22
# 28 frames :24
# 29 frames :20
# 30 frames :16
# 31 frames :9
# 32 frames :12
# 33 frames :11
# 34 frames :5
# 35 frames :6
# 36 frames :5
# 37 frames :4
# 38 frames :3
# 39 frames :5
# 40 frames :3
# 41 frames :5
# 42 frames :3
# 43 frames :2
# 44 frames :1
# 45 frames :4
# 46 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :2
# 55 frames :1
# 67 frames :1
# 76 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7008
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0