Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.39.21 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7ca155c156066b09e9c1468860613ed4",
"name": "M1_20170615_1407_NK_B_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:39:42"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8143 (max 8144) in '8143'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7174
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.25818e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 99 mean_circ: 0.935456
Ran for 3050 frames Cell Events: 183 mean_circ: 0.939048
Ran for 4550 frames Cell Events: 233 mean_circ: 0.940001
Ran for 5050 frames Cell Events: 255 mean_circ: 0.940317
Ran for 7550 frames Cell Events: 374 mean_circ: 0.943094
Ran for 10550 frames Cell Events: 563 mean_circ: 0.943661
Ran for 11050 frames Cell Events: 588 mean_circ: 0.94399
Ran for 11550 frames Cell Events: 609 mean_circ: 0.943743
Ran for 12050 frames Cell Events: 626 mean_circ: 0.944054
Ran for 13550 frames Cell Events: 706 mean_circ: 0.943356
Ran for 14050 frames Cell Events: 735 mean_circ: 0.943261
Ran for 15050 frames Cell Events: 791 mean_circ: 0.943154
Ran for 15550 frames Cell Events: 816 mean_circ: 0.943053
Ran for 16050 frames Cell Events: 854 mean_circ: 0.942527
Ran for 17050 frames Cell Events: 935 mean_circ: 0.942766
Ran for 17550 frames Cell Events: 963 mean_circ: 0.942972
Ran for 18050 frames Cell Events: 995 mean_circ: 0.942994
Ran for 19050 frames Cell Events: 1038 mean_circ: 0.942713
Ran for 19550 frames Cell Events: 1063 mean_circ: 0.942496
Ran for 20050 frames Cell Events: 1089 mean_circ: 0.942188
Ran for 21050 frames Cell Events: 1142 mean_circ: 0.942308
Ran for 22550 frames Cell Events: 1242 mean_circ: 0.941846
Ran for 23550 frames Cell Events: 1284 mean_circ: 0.941785
Ran for 24550 frames Cell Events: 1350 mean_circ: 0.941581
Ran for 25050 frames Cell Events: 1375 mean_circ: 0.941758
Ran for 25550 frames Cell Events: 1399 mean_circ: 0.941733
Ran for 26050 frames Cell Events: 1426 mean_circ: 0.941693
Ran for 27550 frames Cell Events: 1501 mean_circ: 0.941647
Ran for 28550 frames Cell Events: 1563 mean_circ: 0.941887
Ran for 29050 frames Cell Events: 1592 mean_circ: 0.941978
Ran for 29550 frames Cell Events: 1616 mean_circ: 0.942069
Ran for 30550 frames Cell Events: 1675 mean_circ: 0.94234
Ran for 31550 frames Cell Events: 1720 mean_circ: 0.942184
Ran for 32050 frames Cell Events: 1745 mean_circ: 0.942097
Ran for 32550 frames Cell Events: 1777 mean_circ: 0.941938
Ran for 33050 frames Cell Events: 1797 mean_circ: 0.941949
Ran for 33550 frames Cell Events: 1826 mean_circ: 0.941994
Ran for 34050 frames Cell Events: 1853 mean_circ: 0.942106
Ran for 35550 frames Cell Events: 1937 mean_circ: 0.94195
Ran for 37550 frames Cell Events: 2036 mean_circ: 0.941922
Ran for 38050 frames Cell Events: 2062 mean_circ: 0.941807
Ran for 38550 frames Cell Events: 2082 mean_circ: 0.941785
Ran for 39550 frames Cell Events: 2135 mean_circ: 0.941886
Ran for 40050 frames Cell Events: 2162 mean_circ: 0.941878
Ran for 40550 frames Cell Events: 2181 mean_circ: 0.941823
Ran for 41050 frames Cell Events: 2202 mean_circ: 0.941821
Ran for 43550 frames Cell Events: 2362 mean_circ: 0.941918
Ran for 44550 frames Cell Events: 2427 mean_circ: 0.9417
Ran for 45050 frames Cell Events: 2454 mean_circ: 0.941734
Ran for 46050 frames Cell Events: 2501 mean_circ: 0.941812
Ran for 46550 frames Cell Events: 2530 mean_circ: 0.941842
Ran for 47050 frames Cell Events: 2557 mean_circ: 0.941859
Ran for 48550 frames Cell Events: 2641 mean_circ: 0.941586
Ran for 50050 frames Cell Events: 2727 mean_circ: 0.941724
Ran for 51050 frames Cell Events: 2771 mean_circ: 0.94166
Ran for 51550 frames Cell Events: 2802 mean_circ: 0.941752
Ran for 52550 frames Cell Events: 2849 mean_circ: 0.941685
Ran for 53050 frames Cell Events: 2869 mean_circ: 0.941707
Ran for 54050 frames Cell Events: 2920 mean_circ: 0.941642
Ran for 54550 frames Cell Events: 2944 mean_circ: 0.941652
Ran for 55550 frames Cell Events: 3002 mean_circ: 0.941536
Ran for 56050 frames Cell Events: 3029 mean_circ: 0.941505
Ran for 56550 frames Cell Events: 3050 mean_circ: 0.941496
Ran for 58550 frames Cell Events: 3154 mean_circ: 0.941255
Ran for 59550 frames Cell Events: 3203 mean_circ: 0.941101
Ran for 60050 frames Cell Events: 3230 mean_circ: 0.941135
Ran for 60550 frames Cell Events: 3259 mean_circ: 0.941101
Ran for 61050 frames Cell Events: 3286 mean_circ: 0.941157
Ran for 61550 frames Cell Events: 3307 mean_circ: 0.94113
Ran for 62550 frames Cell Events: 3358 mean_circ: 0.941273
Ran for 63050 frames Cell Events: 3377 mean_circ: 0.941252
Ran for 64550 frames Cell Events: 3450 mean_circ: 0.941227
Ran for 65050 frames Cell Events: 3476 mean_circ: 0.941161
Ran for 65550 frames Cell Events: 3490 mean_circ: 0.941191
Ran for 66050 frames Cell Events: 3509 mean_circ: 0.941191
Ran for 67050 frames Cell Events: 3552 mean_circ: 0.941228
Ran for 67550 frames Cell Events: 3594 mean_circ: 0.941228
Ran for 68550 frames Cell Events: 3633 mean_circ: 0.94133
Ran for 69050 frames Cell Events: 3656 mean_circ: 0.941362
Ran for 69550 frames Cell Events: 3673 mean_circ: 0.941374
Ran for 72050 frames Cell Events: 3820 mean_circ: 0.941324
Ran for 74050 frames Cell Events: 3907 mean_circ: 0.941509
Ran for 74550 frames Cell Events: 3939 mean_circ: 0.941549
Ran for 75050 frames Cell Events: 3980 mean_circ: 0.941541
Ran for 75550 frames Cell Events: 4005 mean_circ: 0.941502
Ran for 76550 frames Cell Events: 4066 mean_circ: 0.941352
Ran for 79050 frames Cell Events: 4201 mean_circ: 0.940955
Ran for 80550 frames Cell Events: 4282 mean_circ: 0.940934
Ran for 81550 frames Cell Events: 4316 mean_circ: 0.940996
Ran for 82050 frames Cell Events: 4347 mean_circ: 0.940906
Ran for 82550 frames Cell Events: 4368 mean_circ: 0.940876
Ran for 83050 frames Cell Events: 4389 mean_circ: 0.940963
Ran for 84050 frames Cell Events: 4428 mean_circ: 0.940917
Ran for 85550 frames Cell Events: 4501 mean_circ: 0.941017
Ran for 86050 frames Cell Events: 4530 mean_circ: 0.941043
Ran for 87050 frames Cell Events: 4593 mean_circ: 0.941121
Ran for 87550 frames Cell Events: 4623 mean_circ: 0.941121
Ran for 89050 frames Cell Events: 4710 mean_circ: 0.941021
Ran for 91050 frames Cell Events: 4826 mean_circ: 0.94104
Ran for 91550 frames Cell Events: 4845 mean_circ: 0.94101
Ran for 92550 frames Cell Events: 4903 mean_circ: 0.941011
Ran for 93050 frames Cell Events: 4925 mean_circ: 0.941001
Ran for 94550 frames Cell Events: 5001 mean_circ: 0.940943
Ran for 95050 frames Cell Events: 5024 mean_circ: 0.940955
Ran for 98050 frames Cell Events: 5201 mean_circ: 0.940862
Ran for 100050 frames Cell Events: 5309 mean_circ: 0.940864
Ran for 100550 frames Cell Events: 5338 mean_circ: 0.940923
Ran for 101050 frames Cell Events: 5367 mean_circ: 0.940907
Ran for 101550 frames Cell Events: 5389 mean_circ: 0.940935
Ran for 103550 frames Cell Events: 5510 mean_circ: 0.940967
Ran for 104050 frames Cell Events: 5528 mean_circ: 0.941005
Ran for 104550 frames Cell Events: 5553 mean_circ: 0.941015
Ran for 105050 frames Cell Events: 5587 mean_circ: 0.941011
Ran for 106550 frames Cell Events: 5661 mean_circ: 0.941054
Ran for 108050 frames Cell Events: 5735 mean_circ: 0.941046
Ran for 110550 frames Cell Events: 5853 mean_circ: 0.940971
Ran for 111550 frames Cell Events: 5916 mean_circ: 0.941031
Ran for 112050 frames Cell Events: 5940 mean_circ: 0.940966
Ran for 112550 frames Cell Events: 5961 mean_circ: 0.940939
Ran for 114050 frames Cell Events: 6040 mean_circ: 0.940985
Ran for 115550 frames Cell Events: 6147 mean_circ: 0.940922
Ran for 117550 frames Cell Events: 6268 mean_circ: 0.940833
Ran for 118050 frames Cell Events: 6299 mean_circ: 0.940844
Ran for 118550 frames Cell Events: 6327 mean_circ: 0.940755
Ran for 119050 frames Cell Events: 6354 mean_circ: 0.940767
Ran for 121550 frames Cell Events: 6507 mean_circ: 0.940735
Ran for 122550 frames Cell Events: 6560 mean_circ: 0.940705
Ran for 123550 frames Cell Events: 6607 mean_circ: 0.940683
Ran for 124050 frames Cell Events: 6633 mean_circ: 0.940661
Ran for 124550 frames Cell Events: 6650 mean_circ: 0.940665
Ran for 125050 frames Cell Events: 6678 mean_circ: 0.940705
Ran for 125550 frames Cell Events: 6700 mean_circ: 0.940687
Ran for 126550 frames Cell Events: 6745 mean_circ: 0.940744
Ran for 127050 frames Cell Events: 6766 mean_circ: 0.940777
Ran for 127550 frames Cell Events: 6792 mean_circ: 0.940793
Ran for 128050 frames Cell Events: 6811 mean_circ: 0.940721
Ran for 128550 frames Cell Events: 6829 mean_circ: 0.94064
Ran for 130550 frames Cell Events: 6942 mean_circ: 0.940627
Ran for 135550 frames Cell Events: 7241 mean_circ: 0.940673
Ran for 136550 frames Cell Events: 7297 mean_circ: 0.940597
Ran for 137550 frames Cell Events: 7337 mean_circ: 0.940557
Ran for 138050 frames Cell Events: 7358 mean_circ: 0.940578
Ran for 138550 frames Cell Events: 7381 mean_circ: 0.940577
Ran for 139050 frames Cell Events: 7401 mean_circ: 0.940589
Ran for 139550 frames Cell Events: 7422 mean_circ: 0.940599
Ran for 140050 frames Cell Events: 7442 mean_circ: 0.940584
Ran for 140550 frames Cell Events: 7477 mean_circ: 0.940566
Ran for 141550 frames Cell Events: 7530 mean_circ: 0.940539
Ran for 143050 frames Cell Events: 7611 mean_circ: 0.940596
Ran for 144550 frames Cell Events: 7708 mean_circ: 0.940569
Ran for 145550 frames Cell Events: 7757 mean_circ: 0.940539
Ran for 146550 frames Cell Events: 7816 mean_circ: 0.940554
Ran for 147550 frames Cell Events: 7870 mean_circ: 0.940508
Ran for 148050 frames Cell Events: 7902 mean_circ: 0.940512
Ran for 148550 frames Cell Events: 7919 mean_circ: 0.940459
Ran for 149550 frames Cell Events: 7967 mean_circ: 0.940485
Ran for 151050 frames Cell Events: 8043 mean_circ: 0.940497
Ran for 151550 frames Cell Events: 8076 mean_circ: 0.94049
Ran for 152550 frames Cell Events: 8126 mean_circ: 0.940512



End this measurment..



Stopping time: 1.25895e+008
****************************************
Measurement finished

Mean Circularitiy: 0.94053
Mean Area: 599.899
Mean FL-1 intensity: 3047.57
Mean FL-2 intensity: 3992.23
Mean FL-3 intensity: 0

frames processed: 150553
Total Time: 76473.2ms
Number of Runs (new bg img) 0
Frames collected: 152816
Skipped frames: 7413
Rescued Frames: 936947
Samples DAQ: 38204438
Samples written: 76407742
Samples per Channel: 38203871
FPS: 1998.29

Samples per second: 499572
Expected samples/s: 500000

Events: 8144
Events per second: 106.495
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :231612
# 1 frames :60894
# 2 frames :3168
# 3 frames :1160
# 4 frames :594
# 5 frames :297
# 6 frames :2318
# 7 frames :1190
# 8 frames :991
# 9 frames :567
# 10 frames :511
# 11 frames :400
# 12 frames :286
# 13 frames :182
# 14 frames :254
# 15 frames :188
# 16 frames :155
# 17 frames :128
# 18 frames :98
# 19 frames :59
# 20 frames :59
# 21 frames :55
# 22 frames :52
# 23 frames :46
# 24 frames :33
# 25 frames :27
# 26 frames :18
# 27 frames :15
# 28 frames :17
# 29 frames :16
# 30 frames :18
# 31 frames :10
# 32 frames :13
# 33 frames :9
# 34 frames :5
# 35 frames :12
# 36 frames :5
# 37 frames :4
# 38 frames :6
# 39 frames :3
# 40 frames :2
# 41 frames :3
# 42 frames :2
# 43 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :4
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 53 frames :1
# 54 frames :2
# 58 frames :2
# 62 frames :1
# 64 frames :1
# 72 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7174
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0