Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.40.18 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1517625d3ea1fe88cf27e28d20593494",
"name": "M1_20170615_1419_dsRNA_Y2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:40:42"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9191 (max 9192) in '9191'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6801
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.265e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 156 mean_circ: 0.941701
Ran for 2550 frames Cell Events: 191 mean_circ: 0.938116
Ran for 3050 frames Cell Events: 236 mean_circ: 0.938116
Ran for 3550 frames Cell Events: 260 mean_circ: 0.94013
Ran for 4050 frames Cell Events: 287 mean_circ: 0.94128
Ran for 4550 frames Cell Events: 312 mean_circ: 0.94127
Ran for 5050 frames Cell Events: 351 mean_circ: 0.940632
Ran for 6550 frames Cell Events: 460 mean_circ: 0.939854
Ran for 7050 frames Cell Events: 499 mean_circ: 0.939744
Ran for 8550 frames Cell Events: 624 mean_circ: 0.939866
Ran for 12050 frames Cell Events: 861 mean_circ: 0.939294
Ran for 13050 frames Cell Events: 933 mean_circ: 0.939564
Ran for 14550 frames Cell Events: 1021 mean_circ: 0.939659
Ran for 15550 frames Cell Events: 1081 mean_circ: 0.939903
Ran for 17050 frames Cell Events: 1178 mean_circ: 0.939307
Ran for 18550 frames Cell Events: 1301 mean_circ: 0.939413
Ran for 19050 frames Cell Events: 1331 mean_circ: 0.939434
Ran for 19550 frames Cell Events: 1362 mean_circ: 0.939536
Ran for 20050 frames Cell Events: 1398 mean_circ: 0.939612
Ran for 21050 frames Cell Events: 1451 mean_circ: 0.939875
Ran for 21550 frames Cell Events: 1481 mean_circ: 0.93986
Ran for 22050 frames Cell Events: 1509 mean_circ: 0.94008
Ran for 22550 frames Cell Events: 1542 mean_circ: 0.940141
Ran for 23050 frames Cell Events: 1566 mean_circ: 0.94028
Ran for 23550 frames Cell Events: 1587 mean_circ: 0.940375
Ran for 26050 frames Cell Events: 1771 mean_circ: 0.940232
Ran for 29550 frames Cell Events: 2039 mean_circ: 0.93989
Ran for 33050 frames Cell Events: 2281 mean_circ: 0.940259
Ran for 33550 frames Cell Events: 2319 mean_circ: 0.940353
Ran for 34550 frames Cell Events: 2368 mean_circ: 0.940226
Ran for 35550 frames Cell Events: 2435 mean_circ: 0.940411
Ran for 36050 frames Cell Events: 2465 mean_circ: 0.940564
Ran for 37050 frames Cell Events: 2536 mean_circ: 0.940625
Ran for 38050 frames Cell Events: 2606 mean_circ: 0.940788
Ran for 39550 frames Cell Events: 2712 mean_circ: 0.940846
Ran for 41550 frames Cell Events: 2841 mean_circ: 0.940801
Ran for 45050 frames Cell Events: 3110 mean_circ: 0.941097
Ran for 48550 frames Cell Events: 3354 mean_circ: 0.941601
Ran for 49050 frames Cell Events: 3386 mean_circ: 0.941487
Ran for 51550 frames Cell Events: 3502 mean_circ: 0.941467
Ran for 52550 frames Cell Events: 3579 mean_circ: 0.941387
Ran for 53050 frames Cell Events: 3617 mean_circ: 0.941401
Ran for 54050 frames Cell Events: 3694 mean_circ: 0.941408
Ran for 54550 frames Cell Events: 3720 mean_circ: 0.941411
Ran for 55050 frames Cell Events: 3756 mean_circ: 0.941438
Ran for 56550 frames Cell Events: 3869 mean_circ: 0.941467
Ran for 57550 frames Cell Events: 3913 mean_circ: 0.94149
Ran for 58050 frames Cell Events: 3949 mean_circ: 0.941466
Ran for 60050 frames Cell Events: 4060 mean_circ: 0.941227
Ran for 61050 frames Cell Events: 4131 mean_circ: 0.94107
Ran for 62050 frames Cell Events: 4201 mean_circ: 0.941092
Ran for 63550 frames Cell Events: 4306 mean_circ: 0.941074
Ran for 64550 frames Cell Events: 4397 mean_circ: 0.941061
Ran for 65050 frames Cell Events: 4432 mean_circ: 0.941176
Ran for 65550 frames Cell Events: 4460 mean_circ: 0.941171
Ran for 66050 frames Cell Events: 4490 mean_circ: 0.941169
Ran for 66550 frames Cell Events: 4522 mean_circ: 0.941262
Ran for 67050 frames Cell Events: 4570 mean_circ: 0.941312
Ran for 67550 frames Cell Events: 4609 mean_circ: 0.941279
Ran for 68050 frames Cell Events: 4629 mean_circ: 0.941288
Ran for 69550 frames Cell Events: 4749 mean_circ: 0.941238
Ran for 70050 frames Cell Events: 4785 mean_circ: 0.941252
Ran for 74550 frames Cell Events: 5098 mean_circ: 0.940817
Ran for 79550 frames Cell Events: 5469 mean_circ: 0.940576
Ran for 80550 frames Cell Events: 5554 mean_circ: 0.940528
Ran for 81050 frames Cell Events: 5589 mean_circ: 0.940572
Ran for 82050 frames Cell Events: 5653 mean_circ: 0.940522
Ran for 84550 frames Cell Events: 5802 mean_circ: 0.940564
Ran for 87550 frames Cell Events: 6016 mean_circ: 0.940531
Ran for 88050 frames Cell Events: 6041 mean_circ: 0.940481
Ran for 89050 frames Cell Events: 6119 mean_circ: 0.940491
Ran for 90050 frames Cell Events: 6185 mean_circ: 0.94054
Ran for 90550 frames Cell Events: 6222 mean_circ: 0.940502
Ran for 91050 frames Cell Events: 6251 mean_circ: 0.940469
Ran for 91550 frames Cell Events: 6274 mean_circ: 0.940443
Ran for 92550 frames Cell Events: 6343 mean_circ: 0.940392
Ran for 94050 frames Cell Events: 6425 mean_circ: 0.940373
Ran for 95550 frames Cell Events: 6516 mean_circ: 0.940413
Ran for 96550 frames Cell Events: 6599 mean_circ: 0.940417
Ran for 97050 frames Cell Events: 6638 mean_circ: 0.940446
Ran for 100050 frames Cell Events: 6849 mean_circ: 0.940421
Ran for 100550 frames Cell Events: 6881 mean_circ: 0.940407
Ran for 102550 frames Cell Events: 7033 mean_circ: 0.940392
Ran for 103050 frames Cell Events: 7059 mean_circ: 0.940423
Ran for 103550 frames Cell Events: 7086 mean_circ: 0.940356
Ran for 104050 frames Cell Events: 7108 mean_circ: 0.940368
Ran for 105050 frames Cell Events: 7170 mean_circ: 0.940419
Ran for 105550 frames Cell Events: 7213 mean_circ: 0.940419
Ran for 106050 frames Cell Events: 7250 mean_circ: 0.940473
Ran for 106550 frames Cell Events: 7274 mean_circ: 0.940529
Ran for 109050 frames Cell Events: 7444 mean_circ: 0.940504
Ran for 110550 frames Cell Events: 7549 mean_circ: 0.940473
Ran for 111050 frames Cell Events: 7586 mean_circ: 0.940491
Ran for 111550 frames Cell Events: 7618 mean_circ: 0.940487
Ran for 112550 frames Cell Events: 7669 mean_circ: 0.940502
Ran for 113050 frames Cell Events: 7704 mean_circ: 0.94054
Ran for 113550 frames Cell Events: 7737 mean_circ: 0.940512
Ran for 114550 frames Cell Events: 7817 mean_circ: 0.94047
Ran for 115550 frames Cell Events: 7873 mean_circ: 0.940423
Ran for 117050 frames Cell Events: 7966 mean_circ: 0.940399
Ran for 117550 frames Cell Events: 8003 mean_circ: 0.940405
Ran for 118050 frames Cell Events: 8028 mean_circ: 0.94041
Ran for 118550 frames Cell Events: 8060 mean_circ: 0.940389
Ran for 119050 frames Cell Events: 8094 mean_circ: 0.940394
Ran for 120050 frames Cell Events: 8146 mean_circ: 0.940418
Ran for 122050 frames Cell Events: 8296 mean_circ: 0.940293
Ran for 123550 frames Cell Events: 8385 mean_circ: 0.9403
Ran for 124050 frames Cell Events: 8407 mean_circ: 0.940302
Ran for 124550 frames Cell Events: 8437 mean_circ: 0.940281
Ran for 125050 frames Cell Events: 8475 mean_circ: 0.940282
Ran for 126050 frames Cell Events: 8540 mean_circ: 0.940332
Ran for 127050 frames Cell Events: 8598 mean_circ: 0.940336
Ran for 127550 frames Cell Events: 8634 mean_circ: 0.940317
Ran for 129050 frames Cell Events: 8760 mean_circ: 0.940317
Ran for 129550 frames Cell Events: 8805 mean_circ: 0.940317
Ran for 130050 frames Cell Events: 8844 mean_circ: 0.940332
Ran for 132550 frames Cell Events: 9033 mean_circ: 0.940349
Ran for 133050 frames Cell Events: 9068 mean_circ: 0.940285
Ran for 134550 frames Cell Events: 9169 mean_circ: 0.940358



End this measurment..



Stopping time: 1.26567e+008
****************************************
Measurement finished

Mean Circularitiy: 0.940346
Mean Area: 612.554
Mean FL-1 intensity: 3466.18
Mean FL-2 intensity: 3067.15
Mean FL-3 intensity: 0

frames processed: 133890
Total Time: 67518.4ms
Number of Runs (new bg img) 0
Frames collected: 134890
Skipped frames: 2618
Rescued Frames: 163960
Samples DAQ: 33722755
Samples written: 67444940
Samples per Channel: 33722470
FPS: 1997.82

Samples per second: 499456
Expected samples/s: 500000

Events: 9192
Events per second: 136.141
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :215930
# 1 frames :41997
# 2 frames :2093
# 3 frames :1105
# 4 frames :532
# 5 frames :249
# 6 frames :2324
# 7 frames :890
# 8 frames :764
# 9 frames :596
# 10 frames :684
# 11 frames :404
# 12 frames :233
# 13 frames :232
# 14 frames :253
# 15 frames :193
# 16 frames :197
# 17 frames :153
# 18 frames :125
# 19 frames :85
# 20 frames :101
# 21 frames :69
# 22 frames :65
# 23 frames :54
# 24 frames :52
# 25 frames :38
# 26 frames :34
# 27 frames :23
# 28 frames :28
# 29 frames :21
# 30 frames :26
# 31 frames :19
# 32 frames :15
# 33 frames :13
# 34 frames :12
# 35 frames :10
# 36 frames :10
# 37 frames :7
# 38 frames :5
# 39 frames :4
# 40 frames :7
# 41 frames :6
# 42 frames :3
# 44 frames :1
# 46 frames :2
# 47 frames :2
# 49 frames :2
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 59 frames :1
# 74 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6801
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0