Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.42.19 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4cf8f427a9920abbb94331f7d8f25606",
"name": "M1_20170615_1441_dsRNA_Z2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:42:42"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8858 (max 8859) in '8858'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6847
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.27851e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.927413
Ran for 1050 frames Cell Events: 58 mean_circ: 0.921049
Ran for 2050 frames Cell Events: 123 mean_circ: 0.925461
Ran for 2550 frames Cell Events: 150 mean_circ: 0.918531
Ran for 3550 frames Cell Events: 210 mean_circ: 0.920776
Ran for 4550 frames Cell Events: 295 mean_circ: 0.920776
Ran for 5550 frames Cell Events: 373 mean_circ: 0.913171
Ran for 7050 frames Cell Events: 416 mean_circ: 0.914237
Ran for 7550 frames Cell Events: 438 mean_circ: 0.914527
Ran for 8050 frames Cell Events: 467 mean_circ: 0.914402
Ran for 9550 frames Cell Events: 577 mean_circ: 0.913901
Ran for 10050 frames Cell Events: 605 mean_circ: 0.9145
Ran for 10550 frames Cell Events: 626 mean_circ: 0.914554
Ran for 11550 frames Cell Events: 691 mean_circ: 0.914051
Ran for 17050 frames Cell Events: 1084 mean_circ: 0.914567
Ran for 17550 frames Cell Events: 1099 mean_circ: 0.914867
Ran for 18550 frames Cell Events: 1174 mean_circ: 0.91475
Ran for 20050 frames Cell Events: 1264 mean_circ: 0.914525
Ran for 20550 frames Cell Events: 1289 mean_circ: 0.914396
Ran for 23550 frames Cell Events: 1480 mean_circ: 0.914237
Ran for 24050 frames Cell Events: 1512 mean_circ: 0.913935
Ran for 24550 frames Cell Events: 1535 mean_circ: 0.914068
Ran for 25050 frames Cell Events: 1572 mean_circ: 0.913549
Ran for 25550 frames Cell Events: 1592 mean_circ: 0.913506
Ran for 27550 frames Cell Events: 1721 mean_circ: 0.913562
Ran for 29050 frames Cell Events: 1846 mean_circ: 0.913369
Ran for 31550 frames Cell Events: 2026 mean_circ: 0.913816
Ran for 34050 frames Cell Events: 2214 mean_circ: 0.914169
Ran for 35550 frames Cell Events: 2315 mean_circ: 0.913891
Ran for 36050 frames Cell Events: 2335 mean_circ: 0.913934
Ran for 38050 frames Cell Events: 2458 mean_circ: 0.913985
Ran for 39050 frames Cell Events: 2539 mean_circ: 0.914086
Ran for 41050 frames Cell Events: 2692 mean_circ: 0.913775
Ran for 42550 frames Cell Events: 2785 mean_circ: 0.913603
Ran for 43550 frames Cell Events: 2841 mean_circ: 0.913662
Ran for 44050 frames Cell Events: 2874 mean_circ: 0.913819
Ran for 45050 frames Cell Events: 2945 mean_circ: 0.913642
Ran for 47050 frames Cell Events: 3067 mean_circ: 0.913787
Ran for 51050 frames Cell Events: 3357 mean_circ: 0.913716
Ran for 52550 frames Cell Events: 3426 mean_circ: 0.913928
Ran for 53550 frames Cell Events: 3486 mean_circ: 0.913936
Ran for 54550 frames Cell Events: 3546 mean_circ: 0.91377
Ran for 55050 frames Cell Events: 3576 mean_circ: 0.913847
Ran for 56050 frames Cell Events: 3637 mean_circ: 0.913938
Ran for 57550 frames Cell Events: 3734 mean_circ: 0.913952
Ran for 59050 frames Cell Events: 3844 mean_circ: 0.914267
Ran for 60050 frames Cell Events: 3924 mean_circ: 0.914517
Ran for 60550 frames Cell Events: 3953 mean_circ: 0.914585
Ran for 62550 frames Cell Events: 4120 mean_circ: 0.914588
Ran for 67550 frames Cell Events: 4470 mean_circ: 0.914512
Ran for 71050 frames Cell Events: 4678 mean_circ: 0.9143
Ran for 72050 frames Cell Events: 4733 mean_circ: 0.914149
Ran for 72550 frames Cell Events: 4764 mean_circ: 0.914217
Ran for 73550 frames Cell Events: 4830 mean_circ: 0.914179
Ran for 74550 frames Cell Events: 4919 mean_circ: 0.914192
Ran for 76550 frames Cell Events: 5075 mean_circ: 0.913978
Ran for 77050 frames Cell Events: 5108 mean_circ: 0.914028
Ran for 79550 frames Cell Events: 5285 mean_circ: 0.914026
Ran for 81050 frames Cell Events: 5389 mean_circ: 0.913924
Ran for 82550 frames Cell Events: 5507 mean_circ: 0.913863
Ran for 83550 frames Cell Events: 5568 mean_circ: 0.913834
Ran for 84050 frames Cell Events: 5595 mean_circ: 0.913791
Ran for 84550 frames Cell Events: 5617 mean_circ: 0.913748
Ran for 85050 frames Cell Events: 5652 mean_circ: 0.913689
Ran for 86050 frames Cell Events: 5725 mean_circ: 0.913594
Ran for 86550 frames Cell Events: 5747 mean_circ: 0.913477
Ran for 87050 frames Cell Events: 5767 mean_circ: 0.913503
Ran for 90050 frames Cell Events: 5934 mean_circ: 0.913509
Ran for 91550 frames Cell Events: 6021 mean_circ: 0.913639
Ran for 92050 frames Cell Events: 6057 mean_circ: 0.913608
Ran for 95050 frames Cell Events: 6265 mean_circ: 0.91342
Ran for 96050 frames Cell Events: 6325 mean_circ: 0.913274
Ran for 98550 frames Cell Events: 6497 mean_circ: 0.913267
Ran for 99550 frames Cell Events: 6566 mean_circ: 0.91313
Ran for 101050 frames Cell Events: 6655 mean_circ: 0.913174
Ran for 102550 frames Cell Events: 6756 mean_circ: 0.91321
Ran for 104050 frames Cell Events: 6866 mean_circ: 0.913323
Ran for 105050 frames Cell Events: 6946 mean_circ: 0.913253
Ran for 105550 frames Cell Events: 6978 mean_circ: 0.913173
Ran for 106550 frames Cell Events: 7070 mean_circ: 0.913158
Ran for 107050 frames Cell Events: 7092 mean_circ: 0.913238
Ran for 109050 frames Cell Events: 7240 mean_circ: 0.913257
Ran for 111050 frames Cell Events: 7383 mean_circ: 0.913188
Ran for 111550 frames Cell Events: 7412 mean_circ: 0.913222
Ran for 112550 frames Cell Events: 7473 mean_circ: 0.913126
Ran for 113050 frames Cell Events: 7511 mean_circ: 0.913126
Ran for 113550 frames Cell Events: 7543 mean_circ: 0.913192
Ran for 114050 frames Cell Events: 7584 mean_circ: 0.913117
Ran for 115050 frames Cell Events: 7664 mean_circ: 0.913156
Ran for 115550 frames Cell Events: 7694 mean_circ: 0.913257
Ran for 116050 frames Cell Events: 7715 mean_circ: 0.913235
Ran for 116550 frames Cell Events: 7736 mean_circ: 0.913266
Ran for 118050 frames Cell Events: 7864 mean_circ: 0.913336
Ran for 119050 frames Cell Events: 7921 mean_circ: 0.913378
Ran for 119550 frames Cell Events: 7943 mean_circ: 0.913366
Ran for 120550 frames Cell Events: 8008 mean_circ: 0.913304
Ran for 121550 frames Cell Events: 8081 mean_circ: 0.913284
Ran for 123050 frames Cell Events: 8214 mean_circ: 0.913303
Ran for 123550 frames Cell Events: 8245 mean_circ: 0.913303
Ran for 124550 frames Cell Events: 8297 mean_circ: 0.913416
Ran for 126550 frames Cell Events: 8422 mean_circ: 0.913411
Ran for 127050 frames Cell Events: 8459 mean_circ: 0.913377
Ran for 127550 frames Cell Events: 8494 mean_circ: 0.913392
Ran for 131550 frames Cell Events: 8768 mean_circ: 0.913422



End this measurment..



Stopping time: 1.27918e+008
****************************************
Measurement finished

Mean Circularitiy: 0.913438
Mean Area: 642.248
Mean FL-1 intensity: 3061.86
Mean FL-2 intensity: 2775.25
Mean FL-3 intensity: 0

frames processed: 130501
Total Time: 66441.5ms
Number of Runs (new bg img) 0
Frames collected: 132730
Skipped frames: 8028
Rescued Frames: 514515
Samples DAQ: 33182862
Samples written: 66364722
Samples per Channel: 33182361
FPS: 1997.7

Samples per second: 499422
Expected samples/s: 500000

Events: 8859
Events per second: 133.335
jumped back (PMT): 33
#Frames read, frequency
# 0 frames :218837
# 1 frames :32767
# 2 frames :2290
# 3 frames :988
# 4 frames :633
# 5 frames :366
# 6 frames :1560
# 7 frames :904
# 8 frames :729
# 9 frames :571
# 10 frames :661
# 11 frames :408
# 12 frames :268
# 13 frames :193
# 14 frames :232
# 15 frames :206
# 16 frames :204
# 17 frames :178
# 18 frames :141
# 19 frames :104
# 20 frames :89
# 21 frames :110
# 22 frames :81
# 23 frames :64
# 24 frames :55
# 25 frames :54
# 26 frames :46
# 27 frames :51
# 28 frames :36
# 29 frames :36
# 30 frames :33
# 31 frames :21
# 32 frames :24
# 33 frames :19
# 34 frames :28
# 35 frames :18
# 36 frames :21
# 37 frames :15
# 38 frames :16
# 39 frames :11
# 40 frames :12
# 41 frames :14
# 42 frames :9
# 43 frames :9
# 44 frames :5
# 45 frames :5
# 46 frames :7
# 47 frames :7
# 48 frames :6
# 49 frames :4
# 50 frames :4
# 51 frames :2
# 53 frames :3
# 54 frames :1
# 56 frames :5
# 57 frames :4
# 58 frames :1
# 61 frames :3
# 62 frames :2
# 63 frames :1
# 66 frames :1
# 67 frames :1
# 68 frames :1
# 72 frames :1
# 81 frames :1
# 95 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6847
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0