Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.45.53 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d309adb03ebde3b12f8e0f69e2a7d01a",
"name": "M1_20170615_1607_dsRNA_Y3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:46:18"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10005 (max 10006) in
'10005'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5869
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32982e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.93758
Ran for 1550 frames Cell Events: 100 mean_circ: 0.939634
Ran for 2050 frames Cell Events: 125 mean_circ: 0.938952
Ran for 2550 frames Cell Events: 153 mean_circ: 0.935852
Ran for 4050 frames Cell Events: 245 mean_circ: 0.937019
Ran for 8050 frames Cell Events: 590 mean_circ: 0.93872
Ran for 11050 frames Cell Events: 816 mean_circ: 0.93857
Ran for 11550 frames Cell Events: 850 mean_circ: 0.938701
Ran for 12550 frames Cell Events: 916 mean_circ: 0.938451
Ran for 14550 frames Cell Events: 1047 mean_circ: 0.938008
Ran for 15050 frames Cell Events: 1077 mean_circ: 0.93814
Ran for 15550 frames Cell Events: 1104 mean_circ: 0.938072
Ran for 16550 frames Cell Events: 1147 mean_circ: 0.938258
Ran for 19550 frames Cell Events: 1325 mean_circ: 0.939753
Ran for 20550 frames Cell Events: 1379 mean_circ: 0.940178
Ran for 22050 frames Cell Events: 1494 mean_circ: 0.940354
Ran for 22550 frames Cell Events: 1535 mean_circ: 0.940354
Ran for 23550 frames Cell Events: 1588 mean_circ: 0.940805
Ran for 24550 frames Cell Events: 1654 mean_circ: 0.940635
Ran for 29050 frames Cell Events: 1965 mean_circ: 0.94027
Ran for 30550 frames Cell Events: 2058 mean_circ: 0.940026
Ran for 31050 frames Cell Events: 2097 mean_circ: 0.939991
Ran for 35550 frames Cell Events: 2459 mean_circ: 0.940569
Ran for 36050 frames Cell Events: 2479 mean_circ: 0.940586
Ran for 36550 frames Cell Events: 2504 mean_circ: 0.940592
Ran for 42050 frames Cell Events: 2914 mean_circ: 0.940544
Ran for 44050 frames Cell Events: 3035 mean_circ: 0.940593
Ran for 45550 frames Cell Events: 3123 mean_circ: 0.940699
Ran for 46050 frames Cell Events: 3156 mean_circ: 0.940656
Ran for 50550 frames Cell Events: 3459 mean_circ: 0.940518
Ran for 53050 frames Cell Events: 3635 mean_circ: 0.94049
Ran for 56050 frames Cell Events: 3847 mean_circ: 0.940504
Ran for 56550 frames Cell Events: 3887 mean_circ: 0.940302
Ran for 59550 frames Cell Events: 4133 mean_circ: 0.940343
Ran for 60550 frames Cell Events: 4201 mean_circ: 0.940313
Ran for 65550 frames Cell Events: 4543 mean_circ: 0.94023
Ran for 66050 frames Cell Events: 4576 mean_circ: 0.940195
Ran for 66550 frames Cell Events: 4601 mean_circ: 0.9402
Ran for 67050 frames Cell Events: 4643 mean_circ: 0.940281
Ran for 67550 frames Cell Events: 4674 mean_circ: 0.940282
Ran for 68050 frames Cell Events: 4709 mean_circ: 0.940162
Ran for 70050 frames Cell Events: 4848 mean_circ: 0.940252
Ran for 70550 frames Cell Events: 4884 mean_circ: 0.940086
Ran for 71050 frames Cell Events: 4915 mean_circ: 0.940046
Ran for 71550 frames Cell Events: 4941 mean_circ: 0.940014
Ran for 72050 frames Cell Events: 4979 mean_circ: 0.940116
Ran for 74550 frames Cell Events: 5136 mean_circ: 0.94027
Ran for 78050 frames Cell Events: 5407 mean_circ: 0.940397
Ran for 79550 frames Cell Events: 5531 mean_circ: 0.940318
Ran for 80550 frames Cell Events: 5599 mean_circ: 0.940274
Ran for 81550 frames Cell Events: 5684 mean_circ: 0.94036
Ran for 83050 frames Cell Events: 5820 mean_circ: 0.940424
Ran for 86050 frames Cell Events: 6093 mean_circ: 0.940447
Ran for 86550 frames Cell Events: 6128 mean_circ: 0.940436
Ran for 87050 frames Cell Events: 6154 mean_circ: 0.940402
Ran for 89550 frames Cell Events: 6348 mean_circ: 0.940426
Ran for 90050 frames Cell Events: 6372 mean_circ: 0.940361
Ran for 90550 frames Cell Events: 6402 mean_circ: 0.94024
Ran for 91550 frames Cell Events: 6458 mean_circ: 0.94026
Ran for 92550 frames Cell Events: 6539 mean_circ: 0.940349
Ran for 93050 frames Cell Events: 6577 mean_circ: 0.940375
Ran for 93550 frames Cell Events: 6608 mean_circ: 0.940329
Ran for 95550 frames Cell Events: 6754 mean_circ: 0.940423
Ran for 96050 frames Cell Events: 6786 mean_circ: 0.9405
Ran for 97550 frames Cell Events: 6894 mean_circ: 0.940551
Ran for 99050 frames Cell Events: 7005 mean_circ: 0.940575
Ran for 99550 frames Cell Events: 7030 mean_circ: 0.940583
Ran for 103050 frames Cell Events: 7257 mean_circ: 0.940665
Ran for 103550 frames Cell Events: 7281 mean_circ: 0.940632
Ran for 104050 frames Cell Events: 7312 mean_circ: 0.940649
Ran for 105550 frames Cell Events: 7413 mean_circ: 0.940705
Ran for 106050 frames Cell Events: 7430 mean_circ: 0.940705
Ran for 107550 frames Cell Events: 7495 mean_circ: 0.940808
Ran for 108550 frames Cell Events: 7544 mean_circ: 0.940851
Ran for 109050 frames Cell Events: 7572 mean_circ: 0.940865
Ran for 109550 frames Cell Events: 7592 mean_circ: 0.940854
Ran for 110050 frames Cell Events: 7620 mean_circ: 0.940811
Ran for 111050 frames Cell Events: 7683 mean_circ: 0.94072
Ran for 112050 frames Cell Events: 7746 mean_circ: 0.940639
Ran for 113050 frames Cell Events: 7830 mean_circ: 0.940604
Ran for 116550 frames Cell Events: 8131 mean_circ: 0.940286
Ran for 117050 frames Cell Events: 8161 mean_circ: 0.940252
Ran for 117550 frames Cell Events: 8195 mean_circ: 0.940282
Ran for 118050 frames Cell Events: 8232 mean_circ: 0.940334
Ran for 119050 frames Cell Events: 8299 mean_circ: 0.940367
Ran for 119550 frames Cell Events: 8331 mean_circ: 0.940349
Ran for 120050 frames Cell Events: 8349 mean_circ: 0.940288
Ran for 121550 frames Cell Events: 8446 mean_circ: 0.940241
Ran for 122550 frames Cell Events: 8520 mean_circ: 0.940281
Ran for 124550 frames Cell Events: 8666 mean_circ: 0.940396
Ran for 126050 frames Cell Events: 8764 mean_circ: 0.940405
Ran for 126550 frames Cell Events: 8802 mean_circ: 0.940352
Ran for 129050 frames Cell Events: 8985 mean_circ: 0.940295
Ran for 130550 frames Cell Events: 9094 mean_circ: 0.940225
Ran for 134050 frames Cell Events: 9406 mean_circ: 0.940001
Ran for 139550 frames Cell Events: 9822 mean_circ: 0.940149
Ran for 140050 frames Cell Events: 9854 mean_circ: 0.940154
Ran for 140550 frames Cell Events: 9886 mean_circ: 0.940142
Ran for 141550 frames Cell Events: 9932 mean_circ: 0.940158



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.33053e+008
****************************************
Measurement finished

Mean Circularitiy: 0.940201
Mean Area: 618.797
Mean FL-1 intensity: 3602.71
Mean FL-2 intensity: 3380.99
Mean FL-3 intensity: 0

frames processed: 139250
Total Time: 71301.2ms
Number of Runs (new bg img) 0
Frames collected: 142476
Skipped frames: 21714
Rescued Frames: 1179719
Samples DAQ: 35621079
Samples written: 71238138
Samples per Channel: 35619069
FPS: 1998.23

Samples per second: 499558
Expected samples/s: 500000

Events: 10006
Events per second: 140.334
jumped back (PMT): 2945
#Frames read, frequency
# 0 frames :235899
# 1 frames :35243
# 2 frames :2175
# 3 frames :971
# 4 frames :559
# 5 frames :276
# 6 frames :1293
# 7 frames :946
# 8 frames :740
# 9 frames :478
# 10 frames :654
# 11 frames :385
# 12 frames :282
# 13 frames :230
# 14 frames :209
# 15 frames :257
# 16 frames :239
# 17 frames :187
# 18 frames :150
# 19 frames :119
# 20 frames :97
# 21 frames :112
# 22 frames :93
# 23 frames :95
# 24 frames :83
# 25 frames :51
# 26 frames :53
# 27 frames :72
# 28 frames :58
# 29 frames :46
# 30 frames :40
# 31 frames :41
# 32 frames :39
# 33 frames :41
# 34 frames :39
# 35 frames :19
# 36 frames :22
# 37 frames :19
# 38 frames :13
# 39 frames :19
# 40 frames :12
# 41 frames :13
# 42 frames :20
# 43 frames :4
# 44 frames :14
# 45 frames :8
# 46 frames :9
# 47 frames :7
# 48 frames :8
# 49 frames :9
# 50 frames :4
# 51 frames :5
# 52 frames :3
# 53 frames :3
# 54 frames :6
# 55 frames :6
# 56 frames :3
# 57 frames :2
# 58 frames :7
# 59 frames :3
# 60 frames :2
# 63 frames :2
# 64 frames :1
# 65 frames :1
# 66 frames :2
# 70 frames :1
# 76 frames :1
# 77 frames :1
# 78 frames :2
# 79 frames :2
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5869
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0