Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.46.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "37fff337a0c188e23033f8e2910a203b",
"name": "M1_20170615_1611_dsRNA_Y3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:46:46"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8177 (max 8179) in '8177'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5846
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.3323e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 164 mean_circ: 0.933013
Ran for 3550 frames Cell Events: 233 mean_circ: 0.932447
Ran for 4050 frames Cell Events: 270 mean_circ: 0.93223
Ran for 5050 frames Cell Events: 343 mean_circ: 0.932148
Ran for 5550 frames Cell Events: 375 mean_circ: 0.931158
Ran for 8050 frames Cell Events: 594 mean_circ: 0.930776
Ran for 9550 frames Cell Events: 716 mean_circ: 0.931002
Ran for 10050 frames Cell Events: 764 mean_circ: 0.931473
Ran for 10550 frames Cell Events: 805 mean_circ: 0.930953
Ran for 11050 frames Cell Events: 843 mean_circ: 0.930418
Ran for 13050 frames Cell Events: 1042 mean_circ: 0.931662
Ran for 15550 frames Cell Events: 1188 mean_circ: 0.931394
Ran for 16050 frames Cell Events: 1216 mean_circ: 0.931267
Ran for 16550 frames Cell Events: 1247 mean_circ: 0.93129
Ran for 17550 frames Cell Events: 1316 mean_circ: 0.93101
Ran for 18050 frames Cell Events: 1344 mean_circ: 0.931004
Ran for 19550 frames Cell Events: 1455 mean_circ: 0.930676
Ran for 20050 frames Cell Events: 1509 mean_circ: 0.930676
Ran for 20550 frames Cell Events: 1541 mean_circ: 0.930583
Ran for 21050 frames Cell Events: 1570 mean_circ: 0.930402
Ran for 21550 frames Cell Events: 1620 mean_circ: 0.930402
Ran for 24050 frames Cell Events: 1842 mean_circ: 0.929433
Ran for 24550 frames Cell Events: 1876 mean_circ: 0.929555
Ran for 25050 frames Cell Events: 1908 mean_circ: 0.929499
Ran for 27050 frames Cell Events: 2076 mean_circ: 0.929732
Ran for 27550 frames Cell Events: 2124 mean_circ: 0.929732
Ran for 28050 frames Cell Events: 2172 mean_circ: 0.929553
Ran for 28550 frames Cell Events: 2210 mean_circ: 0.929507
Ran for 29050 frames Cell Events: 2246 mean_circ: 0.929404
Ran for 30050 frames Cell Events: 2348 mean_circ: 0.929528
Ran for 30550 frames Cell Events: 2400 mean_circ: 0.929232
Ran for 32050 frames Cell Events: 2549 mean_circ: 0.92927
Ran for 32550 frames Cell Events: 2571 mean_circ: 0.92949
Ran for 34550 frames Cell Events: 2737 mean_circ: 0.929497
Ran for 35550 frames Cell Events: 2794 mean_circ: 0.929495
Ran for 36050 frames Cell Events: 2836 mean_circ: 0.929503
Ran for 36550 frames Cell Events: 2882 mean_circ: 0.929509
Ran for 40050 frames Cell Events: 3193 mean_circ: 0.929982
Ran for 43050 frames Cell Events: 3435 mean_circ: 0.929979
Ran for 44050 frames Cell Events: 3523 mean_circ: 0.930053
Ran for 45050 frames Cell Events: 3595 mean_circ: 0.930125
Ran for 46050 frames Cell Events: 3650 mean_circ: 0.93001
Ran for 46550 frames Cell Events: 3680 mean_circ: 0.930009
Ran for 48050 frames Cell Events: 3808 mean_circ: 0.929976
Ran for 49550 frames Cell Events: 3923 mean_circ: 0.929963
Ran for 55050 frames Cell Events: 4391 mean_circ: 0.929912
Ran for 56550 frames Cell Events: 4504 mean_circ: 0.930166
Ran for 58050 frames Cell Events: 4597 mean_circ: 0.930195
Ran for 58550 frames Cell Events: 4622 mean_circ: 0.930034
Ran for 60050 frames Cell Events: 4697 mean_circ: 0.929928
Ran for 60550 frames Cell Events: 4742 mean_circ: 0.929913
Ran for 61050 frames Cell Events: 4776 mean_circ: 0.929912
Ran for 62050 frames Cell Events: 4815 mean_circ: 0.929871
Ran for 63050 frames Cell Events: 4884 mean_circ: 0.92993
Ran for 64050 frames Cell Events: 4943 mean_circ: 0.929885
Ran for 64550 frames Cell Events: 4966 mean_circ: 0.929784
Ran for 65050 frames Cell Events: 4996 mean_circ: 0.929741
Ran for 65550 frames Cell Events: 5025 mean_circ: 0.929746
Ran for 66050 frames Cell Events: 5047 mean_circ: 0.929703
Ran for 68050 frames Cell Events: 5170 mean_circ: 0.929677
Ran for 70550 frames Cell Events: 5360 mean_circ: 0.9297
Ran for 71550 frames Cell Events: 5444 mean_circ: 0.929704
Ran for 74050 frames Cell Events: 5635 mean_circ: 0.929469
Ran for 75050 frames Cell Events: 5727 mean_circ: 0.929416
Ran for 77050 frames Cell Events: 5917 mean_circ: 0.929557
Ran for 77550 frames Cell Events: 5944 mean_circ: 0.929532
Ran for 78050 frames Cell Events: 5991 mean_circ: 0.929531
Ran for 80050 frames Cell Events: 6158 mean_circ: 0.929402
Ran for 80550 frames Cell Events: 6185 mean_circ: 0.929391
Ran for 82550 frames Cell Events: 6347 mean_circ: 0.92947
Ran for 83050 frames Cell Events: 6385 mean_circ: 0.92947
Ran for 84550 frames Cell Events: 6507 mean_circ: 0.929162
Ran for 85550 frames Cell Events: 6585 mean_circ: 0.929162
Ran for 86550 frames Cell Events: 6643 mean_circ: 0.92926
Ran for 87550 frames Cell Events: 6697 mean_circ: 0.929249
Ran for 89050 frames Cell Events: 6747 mean_circ: 0.929286
Ran for 89550 frames Cell Events: 6782 mean_circ: 0.92929
Ran for 91550 frames Cell Events: 6956 mean_circ: 0.929196
Ran for 93050 frames Cell Events: 7081 mean_circ: 0.929181
Ran for 94550 frames Cell Events: 7224 mean_circ: 0.929181
Ran for 96050 frames Cell Events: 7332 mean_circ: 0.92932
Ran for 96550 frames Cell Events: 7366 mean_circ: 0.929329
Ran for 99050 frames Cell Events: 7556 mean_circ: 0.929318
Ran for 100050 frames Cell Events: 7637 mean_circ: 0.929347
Ran for 100550 frames Cell Events: 7670 mean_circ: 0.929311
Ran for 102550 frames Cell Events: 7837 mean_circ: 0.929282
Ran for 103050 frames Cell Events: 7875 mean_circ: 0.929294
Ran for 104050 frames Cell Events: 7951 mean_circ: 0.92932



End this measurment..



Stopping time: 1.33284e+008
****************************************
Measurement finished

Mean Circularitiy: 0.929086
Mean Area: 613.706
Mean FL-1 intensity: 3367.12
Mean FL-2 intensity: 3575.81
Mean FL-3 intensity: 0

frames processed: 103871
Total Time: 53544ms
Number of Runs (new bg img) 0
Frames collected: 106885
Skipped frames: 3014
Rescued Frames: 67086
Samples DAQ: 26723240
Samples written: 53442592
Samples per Channel: 26721296
FPS: 1996.21

Samples per second: 499053
Expected samples/s: 500000

Events: 8179
Events per second: 152.753
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :175722
# 1 frames :27507
# 2 frames :1806
# 3 frames :760
# 4 frames :387
# 5 frames :1019
# 6 frames :1055
# 7 frames :709
# 8 frames :434
# 9 frames :393
# 10 frames :401
# 11 frames :304
# 12 frames :240
# 13 frames :236
# 14 frames :214
# 15 frames :177
# 16 frames :154
# 17 frames :113
# 18 frames :107
# 19 frames :93
# 20 frames :82
# 21 frames :67
# 22 frames :59
# 23 frames :52
# 24 frames :47
# 25 frames :40
# 26 frames :31
# 27 frames :36
# 28 frames :40
# 29 frames :28
# 30 frames :19
# 31 frames :10
# 32 frames :28
# 33 frames :23
# 34 frames :12
# 35 frames :13
# 36 frames :10
# 37 frames :8
# 38 frames :7
# 39 frames :5
# 40 frames :11
# 41 frames :10
# 42 frames :3
# 43 frames :5
# 44 frames :5
# 45 frames :2
# 46 frames :5
# 47 frames :4
# 48 frames :6
# 49 frames :5
# 50 frames :3
# 51 frames :3
# 52 frames :1
# 53 frames :2
# 54 frames :2
# 55 frames :2
# 56 frames :4
# 57 frames :1
# 58 frames :1
# 64 frames :2
# 65 frames :1
# 66 frames :2
# 67 frames :2
# 68 frames :1
# 70 frames :1
# 75 frames :2
# 76 frames :1
# 80 frames :1
# 85 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5846
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0