Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.50.36 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6c90de9d8f3f0903ed4bd889fc3a2f2e",
"name": "M1_20170615_1708_dsRNA_Y1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:50:57"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8384 (max 8385) in '8384'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5528
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.3665e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 82 mean_circ: 0.955124
Ran for 2550 frames Cell Events: 183 mean_circ: 0.953326
Ran for 3050 frames Cell Events: 206 mean_circ: 0.95349
Ran for 4550 frames Cell Events: 280 mean_circ: 0.953183
Ran for 6050 frames Cell Events: 376 mean_circ: 0.952842
Ran for 8050 frames Cell Events: 488 mean_circ: 0.953655
Ran for 8550 frames Cell Events: 506 mean_circ: 0.953357
Ran for 9550 frames Cell Events: 574 mean_circ: 0.953249
Ran for 10050 frames Cell Events: 599 mean_circ: 0.953518
Ran for 12550 frames Cell Events: 768 mean_circ: 0.953308
Ran for 14050 frames Cell Events: 855 mean_circ: 0.953042
Ran for 14550 frames Cell Events: 890 mean_circ: 0.952949
Ran for 16050 frames Cell Events: 980 mean_circ: 0.952166
Ran for 16550 frames Cell Events: 1007 mean_circ: 0.951809
Ran for 17050 frames Cell Events: 1029 mean_circ: 0.95198
Ran for 17550 frames Cell Events: 1067 mean_circ: 0.951889
Ran for 18050 frames Cell Events: 1088 mean_circ: 0.952101
Ran for 19050 frames Cell Events: 1153 mean_circ: 0.952207
Ran for 20050 frames Cell Events: 1226 mean_circ: 0.952439
Ran for 20550 frames Cell Events: 1273 mean_circ: 0.952516
Ran for 22050 frames Cell Events: 1362 mean_circ: 0.952298
Ran for 22550 frames Cell Events: 1386 mean_circ: 0.952302
Ran for 23050 frames Cell Events: 1405 mean_circ: 0.952426
Ran for 23550 frames Cell Events: 1435 mean_circ: 0.952308
Ran for 24050 frames Cell Events: 1459 mean_circ: 0.952555
Ran for 24550 frames Cell Events: 1486 mean_circ: 0.952704
Ran for 25050 frames Cell Events: 1512 mean_circ: 0.952689
Ran for 27050 frames Cell Events: 1650 mean_circ: 0.952803
Ran for 27550 frames Cell Events: 1679 mean_circ: 0.952797
Ran for 32050 frames Cell Events: 1957 mean_circ: 0.952267
Ran for 33050 frames Cell Events: 2023 mean_circ: 0.952403
Ran for 34050 frames Cell Events: 2092 mean_circ: 0.952355
Ran for 34550 frames Cell Events: 2129 mean_circ: 0.952301
Ran for 36050 frames Cell Events: 2220 mean_circ: 0.952344
Ran for 36550 frames Cell Events: 2249 mean_circ: 0.952199
Ran for 37550 frames Cell Events: 2326 mean_circ: 0.952279
Ran for 39050 frames Cell Events: 2411 mean_circ: 0.952173
Ran for 39550 frames Cell Events: 2434 mean_circ: 0.952166
Ran for 40050 frames Cell Events: 2453 mean_circ: 0.952172
Ran for 40550 frames Cell Events: 2477 mean_circ: 0.952303
Ran for 41050 frames Cell Events: 2524 mean_circ: 0.95235
Ran for 42550 frames Cell Events: 2636 mean_circ: 0.952452
Ran for 43050 frames Cell Events: 2669 mean_circ: 0.95243
Ran for 44050 frames Cell Events: 2732 mean_circ: 0.952266
Ran for 45050 frames Cell Events: 2775 mean_circ: 0.952291
Ran for 45550 frames Cell Events: 2796 mean_circ: 0.952375
Ran for 46050 frames Cell Events: 2810 mean_circ: 0.952337
Ran for 46550 frames Cell Events: 2834 mean_circ: 0.952362
Ran for 47550 frames Cell Events: 2871 mean_circ: 0.952308
Ran for 48050 frames Cell Events: 2892 mean_circ: 0.952372
Ran for 48550 frames Cell Events: 2919 mean_circ: 0.952357
Ran for 50550 frames Cell Events: 3063 mean_circ: 0.952183
Ran for 51550 frames Cell Events: 3125 mean_circ: 0.9523
Ran for 52550 frames Cell Events: 3169 mean_circ: 0.952388
Ran for 53550 frames Cell Events: 3228 mean_circ: 0.952564
Ran for 54550 frames Cell Events: 3281 mean_circ: 0.952509
Ran for 56050 frames Cell Events: 3351 mean_circ: 0.952669
Ran for 56550 frames Cell Events: 3387 mean_circ: 0.952719
Ran for 57550 frames Cell Events: 3453 mean_circ: 0.952782
Ran for 58050 frames Cell Events: 3481 mean_circ: 0.952813
Ran for 58550 frames Cell Events: 3517 mean_circ: 0.952789
Ran for 59050 frames Cell Events: 3557 mean_circ: 0.952836
Ran for 62050 frames Cell Events: 3744 mean_circ: 0.952987
Ran for 64050 frames Cell Events: 3853 mean_circ: 0.953016
Ran for 65050 frames Cell Events: 3924 mean_circ: 0.952975
Ran for 67050 frames Cell Events: 4038 mean_circ: 0.95285
Ran for 67550 frames Cell Events: 4066 mean_circ: 0.952826
Ran for 68550 frames Cell Events: 4131 mean_circ: 0.952796
Ran for 72050 frames Cell Events: 4346 mean_circ: 0.952797
Ran for 72550 frames Cell Events: 4385 mean_circ: 0.952827
Ran for 73550 frames Cell Events: 4469 mean_circ: 0.952793
Ran for 74050 frames Cell Events: 4496 mean_circ: 0.952799
Ran for 74550 frames Cell Events: 4527 mean_circ: 0.952788
Ran for 75050 frames Cell Events: 4546 mean_circ: 0.952725
Ran for 75550 frames Cell Events: 4562 mean_circ: 0.952746
Ran for 76050 frames Cell Events: 4590 mean_circ: 0.952612
Ran for 77550 frames Cell Events: 4689 mean_circ: 0.952582
Ran for 78050 frames Cell Events: 4728 mean_circ: 0.952538
Ran for 78550 frames Cell Events: 4776 mean_circ: 0.952433
Ran for 79050 frames Cell Events: 4790 mean_circ: 0.952417
Ran for 80050 frames Cell Events: 4879 mean_circ: 0.952457
Ran for 80550 frames Cell Events: 4925 mean_circ: 0.952439
Ran for 81550 frames Cell Events: 4986 mean_circ: 0.952414
Ran for 82550 frames Cell Events: 5046 mean_circ: 0.95242
Ran for 83050 frames Cell Events: 5072 mean_circ: 0.952452
Ran for 84050 frames Cell Events: 5137 mean_circ: 0.952443
Ran for 85050 frames Cell Events: 5184 mean_circ: 0.952406
Ran for 86050 frames Cell Events: 5256 mean_circ: 0.952447
Ran for 86550 frames Cell Events: 5283 mean_circ: 0.952467
Ran for 89050 frames Cell Events: 5462 mean_circ: 0.952585
Ran for 91050 frames Cell Events: 5572 mean_circ: 0.952604
Ran for 92050 frames Cell Events: 5619 mean_circ: 0.952613
Ran for 92550 frames Cell Events: 5648 mean_circ: 0.952567
Ran for 94550 frames Cell Events: 5783 mean_circ: 0.952447
Ran for 95050 frames Cell Events: 5816 mean_circ: 0.952509
Ran for 96550 frames Cell Events: 5914 mean_circ: 0.952356
Ran for 98550 frames Cell Events: 6031 mean_circ: 0.952381
Ran for 99050 frames Cell Events: 6058 mean_circ: 0.952377
Ran for 100550 frames Cell Events: 6150 mean_circ: 0.952422
Ran for 101050 frames Cell Events: 6174 mean_circ: 0.952429
Ran for 103050 frames Cell Events: 6285 mean_circ: 0.952309
Ran for 104050 frames Cell Events: 6346 mean_circ: 0.952307
Ran for 104550 frames Cell Events: 6378 mean_circ: 0.952297
Ran for 106050 frames Cell Events: 6462 mean_circ: 0.952274
Ran for 107050 frames Cell Events: 6507 mean_circ: 0.952286
Ran for 107550 frames Cell Events: 6535 mean_circ: 0.952283
Ran for 108550 frames Cell Events: 6599 mean_circ: 0.952302
Ran for 110050 frames Cell Events: 6675 mean_circ: 0.952315
Ran for 111050 frames Cell Events: 6746 mean_circ: 0.952232
Ran for 112550 frames Cell Events: 6837 mean_circ: 0.952269
Ran for 114550 frames Cell Events: 6983 mean_circ: 0.952194
Ran for 115550 frames Cell Events: 7057 mean_circ: 0.952212
Ran for 116050 frames Cell Events: 7092 mean_circ: 0.952211
Ran for 116550 frames Cell Events: 7119 mean_circ: 0.952212
Ran for 119550 frames Cell Events: 7341 mean_circ: 0.952148
Ran for 120050 frames Cell Events: 7371 mean_circ: 0.952164
Ran for 121050 frames Cell Events: 7449 mean_circ: 0.95218
Ran for 121550 frames Cell Events: 7472 mean_circ: 0.952177
Ran for 122050 frames Cell Events: 7519 mean_circ: 0.952177
Ran for 122550 frames Cell Events: 7552 mean_circ: 0.952166
Ran for 123050 frames Cell Events: 7574 mean_circ: 0.952174
Ran for 123550 frames Cell Events: 7594 mean_circ: 0.952186
Ran for 124050 frames Cell Events: 7614 mean_circ: 0.952188
Ran for 124550 frames Cell Events: 7643 mean_circ: 0.95224
Ran for 125050 frames Cell Events: 7674 mean_circ: 0.952237
Ran for 128050 frames Cell Events: 7909 mean_circ: 0.952256
Ran for 130550 frames Cell Events: 8082 mean_circ: 0.952229
Ran for 131050 frames Cell Events: 8113 mean_circ: 0.952216
Ran for 133050 frames Cell Events: 8286 mean_circ: 0.95215
Ran for 134050 frames Cell Events: 8339 mean_circ: 0.952148
Ran for 134550 frames Cell Events: 8368 mean_circ: 0.952123



End this measurment..



Stopping time: 1.36717e+008
****************************************
Measurement finished

Mean Circularitiy: 0.952111
Mean Area: 656.313
Mean FL-1 intensity: 3400.87
Mean FL-2 intensity: 2949.09
Mean FL-3 intensity: 0

frames processed: 134725
Total Time: 67603.6ms
Number of Runs (new bg img) 0
Frames collected: 135000
Skipped frames: 9382
Rescued Frames: 795166
Samples DAQ: 33750088
Samples written: 67499778
Samples per Channel: 33749889
FPS: 1996.94

Samples per second: 499232
Expected samples/s: 500000

Events: 8385
Events per second: 124.032
jumped back (PMT): 4218
#Frames read, frequency
# 0 frames :207935
# 1 frames :48811
# 2 frames :3526
# 3 frames :1222
# 4 frames :541
# 5 frames :1733
# 6 frames :1477
# 7 frames :963
# 8 frames :545
# 9 frames :422
# 10 frames :632
# 11 frames :443
# 12 frames :342
# 13 frames :223
# 14 frames :185
# 15 frames :153
# 16 frames :119
# 17 frames :100
# 18 frames :96
# 19 frames :71
# 20 frames :72
# 21 frames :54
# 22 frames :57
# 23 frames :29
# 24 frames :28
# 25 frames :34
# 26 frames :26
# 27 frames :18
# 28 frames :20
# 29 frames :14
# 30 frames :14
# 31 frames :5
# 32 frames :8
# 33 frames :9
# 34 frames :10
# 35 frames :4
# 36 frames :6
# 37 frames :6
# 38 frames :3
# 39 frames :1
# 40 frames :1
# 41 frames :2
# 43 frames :1
# 45 frames :1
# 46 frames :2
# 48 frames :1
# 50 frames :3
# 52 frames :1
# 53 frames :2
# 55 frames :1
# 62 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5528
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0