Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.54.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "839a94801d5c93244488ad7b79ca77ad",
"name": "M1_20170615_1806_dsRNA_W1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:54:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8378 (max 8380) in '8378'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6392
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.40157e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 37 mean_circ: 0.907679
Ran for 2050 frames Cell Events: 170 mean_circ: 0.928124
Ran for 3050 frames Cell Events: 240 mean_circ: 0.934161
Ran for 3550 frames Cell Events: 274 mean_circ: 0.933604
Ran for 5550 frames Cell Events: 443 mean_circ: 0.935673
Ran for 6050 frames Cell Events: 487 mean_circ: 0.935116
Ran for 6550 frames Cell Events: 525 mean_circ: 0.935742
Ran for 7050 frames Cell Events: 556 mean_circ: 0.93645
Ran for 8050 frames Cell Events: 601 mean_circ: 0.936326
Ran for 9050 frames Cell Events: 669 mean_circ: 0.936122
Ran for 10050 frames Cell Events: 721 mean_circ: 0.936435
Ran for 10550 frames Cell Events: 758 mean_circ: 0.936696
Ran for 12050 frames Cell Events: 847 mean_circ: 0.937054
Ran for 12550 frames Cell Events: 863 mean_circ: 0.937293
Ran for 14050 frames Cell Events: 961 mean_circ: 0.937377
Ran for 16050 frames Cell Events: 1092 mean_circ: 0.93772
Ran for 17050 frames Cell Events: 1153 mean_circ: 0.937685
Ran for 18050 frames Cell Events: 1230 mean_circ: 0.938065
Ran for 19050 frames Cell Events: 1304 mean_circ: 0.937943
Ran for 19550 frames Cell Events: 1331 mean_circ: 0.938087
Ran for 20550 frames Cell Events: 1382 mean_circ: 0.938411
Ran for 21050 frames Cell Events: 1400 mean_circ: 0.938389
Ran for 21550 frames Cell Events: 1430 mean_circ: 0.938535
Ran for 22050 frames Cell Events: 1450 mean_circ: 0.938492
Ran for 23550 frames Cell Events: 1566 mean_circ: 0.938694
Ran for 24550 frames Cell Events: 1639 mean_circ: 0.938872
Ran for 25050 frames Cell Events: 1672 mean_circ: 0.938996
Ran for 26050 frames Cell Events: 1727 mean_circ: 0.939046
Ran for 26550 frames Cell Events: 1765 mean_circ: 0.938847
Ran for 27050 frames Cell Events: 1794 mean_circ: 0.938875
Ran for 27550 frames Cell Events: 1820 mean_circ: 0.93886
Ran for 28050 frames Cell Events: 1853 mean_circ: 0.93889
Ran for 29550 frames Cell Events: 1971 mean_circ: 0.938908
Ran for 30550 frames Cell Events: 2058 mean_circ: 0.938908
Ran for 32050 frames Cell Events: 2145 mean_circ: 0.938986
Ran for 32550 frames Cell Events: 2185 mean_circ: 0.939093
Ran for 33050 frames Cell Events: 2233 mean_circ: 0.939238
Ran for 34550 frames Cell Events: 2335 mean_circ: 0.939324
Ran for 36550 frames Cell Events: 2436 mean_circ: 0.939251
Ran for 37550 frames Cell Events: 2519 mean_circ: 0.939363
Ran for 38050 frames Cell Events: 2548 mean_circ: 0.939433
Ran for 38550 frames Cell Events: 2566 mean_circ: 0.939283
Ran for 39050 frames Cell Events: 2592 mean_circ: 0.939288
Ran for 40050 frames Cell Events: 2652 mean_circ: 0.939385
Ran for 40550 frames Cell Events: 2688 mean_circ: 0.93942
Ran for 41550 frames Cell Events: 2748 mean_circ: 0.939255
Ran for 42050 frames Cell Events: 2778 mean_circ: 0.939197
Ran for 42550 frames Cell Events: 2807 mean_circ: 0.939233
Ran for 43050 frames Cell Events: 2832 mean_circ: 0.939259
Ran for 43550 frames Cell Events: 2852 mean_circ: 0.939329
Ran for 44050 frames Cell Events: 2884 mean_circ: 0.939185
Ran for 46050 frames Cell Events: 2999 mean_circ: 0.939309
Ran for 47050 frames Cell Events: 3055 mean_circ: 0.93921
Ran for 48550 frames Cell Events: 3155 mean_circ: 0.939283
Ran for 49050 frames Cell Events: 3184 mean_circ: 0.939126
Ran for 49550 frames Cell Events: 3210 mean_circ: 0.939196
Ran for 50050 frames Cell Events: 3235 mean_circ: 0.939281
Ran for 50550 frames Cell Events: 3248 mean_circ: 0.939309
Ran for 52050 frames Cell Events: 3351 mean_circ: 0.939361
Ran for 54550 frames Cell Events: 3504 mean_circ: 0.939533
Ran for 55050 frames Cell Events: 3538 mean_circ: 0.939549
Ran for 56050 frames Cell Events: 3613 mean_circ: 0.939644
Ran for 56550 frames Cell Events: 3644 mean_circ: 0.93967
Ran for 57550 frames Cell Events: 3715 mean_circ: 0.939512
Ran for 58550 frames Cell Events: 3775 mean_circ: 0.939577
Ran for 59550 frames Cell Events: 3840 mean_circ: 0.939613
Ran for 60050 frames Cell Events: 3865 mean_circ: 0.93967
Ran for 60550 frames Cell Events: 3889 mean_circ: 0.939577
Ran for 61050 frames Cell Events: 3909 mean_circ: 0.93963
Ran for 62050 frames Cell Events: 3974 mean_circ: 0.939615
Ran for 62550 frames Cell Events: 3992 mean_circ: 0.939577
Ran for 64050 frames Cell Events: 4080 mean_circ: 0.939502
Ran for 65050 frames Cell Events: 4163 mean_circ: 0.939508
Ran for 65550 frames Cell Events: 4183 mean_circ: 0.939483
Ran for 66550 frames Cell Events: 4251 mean_circ: 0.939522
Ran for 67050 frames Cell Events: 4293 mean_circ: 0.939546
Ran for 67550 frames Cell Events: 4319 mean_circ: 0.939603
Ran for 68050 frames Cell Events: 4346 mean_circ: 0.939631
Ran for 70050 frames Cell Events: 4516 mean_circ: 0.939597
Ran for 71050 frames Cell Events: 4569 mean_circ: 0.93959
Ran for 72050 frames Cell Events: 4644 mean_circ: 0.939543
Ran for 73550 frames Cell Events: 4790 mean_circ: 0.939476
Ran for 74050 frames Cell Events: 4821 mean_circ: 0.939446
Ran for 75050 frames Cell Events: 4895 mean_circ: 0.939412
Ran for 76050 frames Cell Events: 4960 mean_circ: 0.939344
Ran for 77050 frames Cell Events: 5049 mean_circ: 0.939193
Ran for 77550 frames Cell Events: 5080 mean_circ: 0.939099
Ran for 78550 frames Cell Events: 5156 mean_circ: 0.939108
Ran for 79050 frames Cell Events: 5182 mean_circ: 0.939136
Ran for 79550 frames Cell Events: 5230 mean_circ: 0.939136
Ran for 80550 frames Cell Events: 5303 mean_circ: 0.939132
Ran for 81050 frames Cell Events: 5326 mean_circ: 0.939148
Ran for 83550 frames Cell Events: 5538 mean_circ: 0.939037
Ran for 84550 frames Cell Events: 5590 mean_circ: 0.93901
Ran for 85050 frames Cell Events: 5614 mean_circ: 0.938965
Ran for 86050 frames Cell Events: 5666 mean_circ: 0.938915
Ran for 87050 frames Cell Events: 5755 mean_circ: 0.938939
Ran for 89550 frames Cell Events: 5944 mean_circ: 0.938831
Ran for 90550 frames Cell Events: 6016 mean_circ: 0.938923
Ran for 91050 frames Cell Events: 6040 mean_circ: 0.938958
Ran for 92050 frames Cell Events: 6114 mean_circ: 0.938947
Ran for 93050 frames Cell Events: 6184 mean_circ: 0.938969
Ran for 93550 frames Cell Events: 6222 mean_circ: 0.938911
Ran for 94050 frames Cell Events: 6248 mean_circ: 0.938895
Ran for 94550 frames Cell Events: 6278 mean_circ: 0.938892
Ran for 95550 frames Cell Events: 6348 mean_circ: 0.938875
Ran for 97050 frames Cell Events: 6470 mean_circ: 0.938833
Ran for 98550 frames Cell Events: 6567 mean_circ: 0.938762
Ran for 99050 frames Cell Events: 6595 mean_circ: 0.93881
Ran for 99550 frames Cell Events: 6618 mean_circ: 0.938806
Ran for 100550 frames Cell Events: 6694 mean_circ: 0.938716
Ran for 102050 frames Cell Events: 6798 mean_circ: 0.938639
Ran for 102550 frames Cell Events: 6832 mean_circ: 0.938658
Ran for 103050 frames Cell Events: 6861 mean_circ: 0.938699
Ran for 104050 frames Cell Events: 6926 mean_circ: 0.938657
Ran for 104550 frames Cell Events: 6957 mean_circ: 0.938677
Ran for 106050 frames Cell Events: 7054 mean_circ: 0.938598
Ran for 108050 frames Cell Events: 7188 mean_circ: 0.938671
Ran for 110050 frames Cell Events: 7363 mean_circ: 0.938541
Ran for 112050 frames Cell Events: 7524 mean_circ: 0.93862
Ran for 112550 frames Cell Events: 7548 mean_circ: 0.938646
Ran for 113050 frames Cell Events: 7575 mean_circ: 0.938707
Ran for 114050 frames Cell Events: 7676 mean_circ: 0.938741
Ran for 114550 frames Cell Events: 7700 mean_circ: 0.938697
Ran for 115050 frames Cell Events: 7726 mean_circ: 0.938714
Ran for 115550 frames Cell Events: 7759 mean_circ: 0.938719
Ran for 116050 frames Cell Events: 7791 mean_circ: 0.938611
Ran for 116550 frames Cell Events: 7819 mean_circ: 0.938634
Ran for 117050 frames Cell Events: 7847 mean_circ: 0.938638
Ran for 117550 frames Cell Events: 7873 mean_circ: 0.938596
Ran for 118550 frames Cell Events: 7946 mean_circ: 0.938545
Ran for 119550 frames Cell Events: 7997 mean_circ: 0.938554
Ran for 120050 frames Cell Events: 8032 mean_circ: 0.938515
Ran for 120550 frames Cell Events: 8070 mean_circ: 0.938538
Ran for 121050 frames Cell Events: 8099 mean_circ: 0.938495
Ran for 122050 frames Cell Events: 8149 mean_circ: 0.938489
Ran for 122550 frames Cell Events: 8170 mean_circ: 0.938456
Ran for 123550 frames Cell Events: 8249 mean_circ: 0.938377
Ran for 125050 frames Cell Events: 8344 mean_circ: 0.938374



End this measurment..



Stopping time: 1.4022e+008
****************************************
Measurement finished

Mean Circularitiy: 0.938371
Mean Area: 626.65
Mean FL-1 intensity: 5420.06
Mean FL-2 intensity: 5403.38
Mean FL-3 intensity: 0

frames processed: 125317
Total Time: 62825.6ms
Number of Runs (new bg img) 0
Frames collected: 125537
Skipped frames: 14565
Rescued Frames: 1344327
Samples DAQ: 31389336
Samples written: 62768306
Samples per Channel: 31384153
FPS: 1998.18

Samples per second: 499544
Expected samples/s: 500000

Events: 8380
Events per second: 133.385
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :192405
# 1 frames :46691
# 2 frames :2557
# 3 frames :1086
# 4 frames :418
# 5 frames :2275
# 6 frames :1033
# 7 frames :1006
# 8 frames :528
# 9 frames :358
# 10 frames :411
# 11 frames :513
# 12 frames :368
# 13 frames :229
# 14 frames :190
# 15 frames :134
# 16 frames :106
# 17 frames :79
# 18 frames :71
# 19 frames :58
# 20 frames :60
# 21 frames :44
# 22 frames :38
# 23 frames :38
# 24 frames :30
# 25 frames :30
# 26 frames :17
# 27 frames :20
# 28 frames :17
# 29 frames :15
# 30 frames :11
# 31 frames :6
# 32 frames :19
# 33 frames :1
# 34 frames :6
# 35 frames :5
# 36 frames :2
# 38 frames :4
# 39 frames :2
# 40 frames :4
# 44 frames :1
# 47 frames :1
# 52 frames :1
# 55 frames :2
# 58 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6392
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0