Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.59.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0655b470c0126264eeae3449ff365346",
"name": "M1_20170615_1914_dsRNA_X7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:59:44"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8372 (max 8373) in '8372'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5302
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.44223e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 62 mean_circ: 0.939219
Ran for 2550 frames Cell Events: 177 mean_circ: 0.943503
Ran for 3550 frames Cell Events: 256 mean_circ: 0.943593
Ran for 4050 frames Cell Events: 281 mean_circ: 0.943817
Ran for 6050 frames Cell Events: 411 mean_circ: 0.943146
Ran for 6550 frames Cell Events: 444 mean_circ: 0.943184
Ran for 8550 frames Cell Events: 558 mean_circ: 0.943492
Ran for 10050 frames Cell Events: 643 mean_circ: 0.943815
Ran for 10550 frames Cell Events: 678 mean_circ: 0.943452
Ran for 12550 frames Cell Events: 806 mean_circ: 0.943684
Ran for 13050 frames Cell Events: 833 mean_circ: 0.943677
Ran for 13550 frames Cell Events: 867 mean_circ: 0.943358
Ran for 14050 frames Cell Events: 897 mean_circ: 0.9432
Ran for 14550 frames Cell Events: 935 mean_circ: 0.942481
Ran for 15550 frames Cell Events: 995 mean_circ: 0.941764
Ran for 16550 frames Cell Events: 1048 mean_circ: 0.942123
Ran for 18050 frames Cell Events: 1165 mean_circ: 0.941863
Ran for 19050 frames Cell Events: 1239 mean_circ: 0.942293
Ran for 20550 frames Cell Events: 1333 mean_circ: 0.942381
Ran for 21550 frames Cell Events: 1380 mean_circ: 0.942871
Ran for 23050 frames Cell Events: 1489 mean_circ: 0.942657
Ran for 24550 frames Cell Events: 1606 mean_circ: 0.942792
Ran for 26050 frames Cell Events: 1683 mean_circ: 0.94283
Ran for 26550 frames Cell Events: 1709 mean_circ: 0.942467
Ran for 27550 frames Cell Events: 1779 mean_circ: 0.942205
Ran for 28050 frames Cell Events: 1806 mean_circ: 0.942278
Ran for 28550 frames Cell Events: 1835 mean_circ: 0.942481
Ran for 29550 frames Cell Events: 1919 mean_circ: 0.942565
Ran for 30050 frames Cell Events: 1950 mean_circ: 0.942621
Ran for 31550 frames Cell Events: 2014 mean_circ: 0.942521
Ran for 34050 frames Cell Events: 2176 mean_circ: 0.942733
Ran for 35550 frames Cell Events: 2273 mean_circ: 0.94296
Ran for 36550 frames Cell Events: 2329 mean_circ: 0.942974
Ran for 37050 frames Cell Events: 2356 mean_circ: 0.943038
Ran for 38050 frames Cell Events: 2418 mean_circ: 0.942968
Ran for 39550 frames Cell Events: 2540 mean_circ: 0.942821
Ran for 40050 frames Cell Events: 2570 mean_circ: 0.942843
Ran for 40550 frames Cell Events: 2598 mean_circ: 0.94284
Ran for 41550 frames Cell Events: 2683 mean_circ: 0.942875
Ran for 42050 frames Cell Events: 2715 mean_circ: 0.942888
Ran for 42550 frames Cell Events: 2736 mean_circ: 0.94293
Ran for 43050 frames Cell Events: 2768 mean_circ: 0.942957
Ran for 44050 frames Cell Events: 2815 mean_circ: 0.943145
Ran for 44550 frames Cell Events: 2841 mean_circ: 0.943249
Ran for 45050 frames Cell Events: 2867 mean_circ: 0.943185
Ran for 45550 frames Cell Events: 2895 mean_circ: 0.943238
Ran for 46550 frames Cell Events: 2949 mean_circ: 0.943272
Ran for 47050 frames Cell Events: 2973 mean_circ: 0.943249
Ran for 48050 frames Cell Events: 3026 mean_circ: 0.943283
Ran for 48550 frames Cell Events: 3045 mean_circ: 0.943244
Ran for 49050 frames Cell Events: 3078 mean_circ: 0.943273
Ran for 50550 frames Cell Events: 3214 mean_circ: 0.943322
Ran for 51050 frames Cell Events: 3244 mean_circ: 0.943334
Ran for 51550 frames Cell Events: 3275 mean_circ: 0.943367
Ran for 52050 frames Cell Events: 3311 mean_circ: 0.943297
Ran for 53050 frames Cell Events: 3362 mean_circ: 0.943387
Ran for 53550 frames Cell Events: 3401 mean_circ: 0.943393
Ran for 54050 frames Cell Events: 3426 mean_circ: 0.94331
Ran for 55050 frames Cell Events: 3484 mean_circ: 0.943198
Ran for 57050 frames Cell Events: 3635 mean_circ: 0.943161
Ran for 57550 frames Cell Events: 3671 mean_circ: 0.94328
Ran for 59550 frames Cell Events: 3787 mean_circ: 0.943368
Ran for 60050 frames Cell Events: 3812 mean_circ: 0.943443
Ran for 61550 frames Cell Events: 3921 mean_circ: 0.94361
Ran for 63550 frames Cell Events: 4047 mean_circ: 0.94353
Ran for 64050 frames Cell Events: 4086 mean_circ: 0.943626
Ran for 65050 frames Cell Events: 4144 mean_circ: 0.943548
Ran for 67050 frames Cell Events: 4289 mean_circ: 0.943358
Ran for 67550 frames Cell Events: 4324 mean_circ: 0.943278
Ran for 68050 frames Cell Events: 4349 mean_circ: 0.943331
Ran for 69050 frames Cell Events: 4410 mean_circ: 0.943313
Ran for 70050 frames Cell Events: 4451 mean_circ: 0.943398
Ran for 71050 frames Cell Events: 4516 mean_circ: 0.943345
Ran for 71550 frames Cell Events: 4544 mean_circ: 0.943374
Ran for 72550 frames Cell Events: 4612 mean_circ: 0.943407
Ran for 73050 frames Cell Events: 4641 mean_circ: 0.943343
Ran for 73550 frames Cell Events: 4663 mean_circ: 0.943389
Ran for 74050 frames Cell Events: 4680 mean_circ: 0.943353
Ran for 74550 frames Cell Events: 4711 mean_circ: 0.943373
Ran for 76050 frames Cell Events: 4808 mean_circ: 0.943271
Ran for 76550 frames Cell Events: 4821 mean_circ: 0.9433
Ran for 77050 frames Cell Events: 4851 mean_circ: 0.943298
Ran for 78550 frames Cell Events: 4953 mean_circ: 0.943388
Ran for 80050 frames Cell Events: 5098 mean_circ: 0.94329
Ran for 80550 frames Cell Events: 5136 mean_circ: 0.943353
Ran for 81550 frames Cell Events: 5223 mean_circ: 0.94337
Ran for 82050 frames Cell Events: 5247 mean_circ: 0.943391
Ran for 84050 frames Cell Events: 5399 mean_circ: 0.943426
Ran for 84550 frames Cell Events: 5422 mean_circ: 0.943386
Ran for 85550 frames Cell Events: 5489 mean_circ: 0.94337
Ran for 86550 frames Cell Events: 5556 mean_circ: 0.943278
Ran for 87550 frames Cell Events: 5631 mean_circ: 0.943341
Ran for 89050 frames Cell Events: 5731 mean_circ: 0.943239
Ran for 89550 frames Cell Events: 5755 mean_circ: 0.943229
Ran for 93550 frames Cell Events: 6030 mean_circ: 0.943274
Ran for 94050 frames Cell Events: 6057 mean_circ: 0.943262
Ran for 94550 frames Cell Events: 6098 mean_circ: 0.943224
Ran for 95050 frames Cell Events: 6135 mean_circ: 0.943175
Ran for 96050 frames Cell Events: 6195 mean_circ: 0.94316
Ran for 97550 frames Cell Events: 6289 mean_circ: 0.943089
Ran for 98050 frames Cell Events: 6318 mean_circ: 0.943105
Ran for 99050 frames Cell Events: 6389 mean_circ: 0.94313
Ran for 100050 frames Cell Events: 6469 mean_circ: 0.943141
Ran for 100550 frames Cell Events: 6502 mean_circ: 0.943117
Ran for 101050 frames Cell Events: 6543 mean_circ: 0.943032
Ran for 101550 frames Cell Events: 6577 mean_circ: 0.942983
Ran for 103050 frames Cell Events: 6675 mean_circ: 0.942891
Ran for 103550 frames Cell Events: 6705 mean_circ: 0.942843
Ran for 104050 frames Cell Events: 6728 mean_circ: 0.94287
Ran for 104550 frames Cell Events: 6760 mean_circ: 0.942869
Ran for 105550 frames Cell Events: 6851 mean_circ: 0.942874
Ran for 107050 frames Cell Events: 6980 mean_circ: 0.942709
Ran for 107550 frames Cell Events: 7010 mean_circ: 0.942742
Ran for 108050 frames Cell Events: 7037 mean_circ: 0.942727
Ran for 109050 frames Cell Events: 7106 mean_circ: 0.942719
Ran for 109550 frames Cell Events: 7128 mean_circ: 0.942698
Ran for 110050 frames Cell Events: 7150 mean_circ: 0.942686
Ran for 110550 frames Cell Events: 7170 mean_circ: 0.942692
Ran for 111550 frames Cell Events: 7244 mean_circ: 0.942731
Ran for 113550 frames Cell Events: 7355 mean_circ: 0.942735
Ran for 115050 frames Cell Events: 7451 mean_circ: 0.942788
Ran for 115550 frames Cell Events: 7487 mean_circ: 0.942731
Ran for 116550 frames Cell Events: 7570 mean_circ: 0.942703
Ran for 117550 frames Cell Events: 7649 mean_circ: 0.942687
Ran for 118550 frames Cell Events: 7755 mean_circ: 0.942642
Ran for 119050 frames Cell Events: 7786 mean_circ: 0.942565
Ran for 119550 frames Cell Events: 7819 mean_circ: 0.94261
Ran for 120050 frames Cell Events: 7854 mean_circ: 0.942572
Ran for 120550 frames Cell Events: 7874 mean_circ: 0.942629
Ran for 121050 frames Cell Events: 7904 mean_circ: 0.942615
Ran for 124050 frames Cell Events: 8132 mean_circ: 0.942595
Ran for 124550 frames Cell Events: 8164 mean_circ: 0.94261
Ran for 126050 frames Cell Events: 8248 mean_circ: 0.942576
Ran for 126550 frames Cell Events: 8274 mean_circ: 0.942552



End this measurment..



Stopping time: 1.44287e+008
****************************************
Measurement finished

Mean Circularitiy: 0.942498
Mean Area: 646.517
Mean FL-1 intensity: 3440.08
Mean FL-2 intensity: 2835.93
Mean FL-3 intensity: 0

frames processed: 127633
Total Time: 64108.3ms
Number of Runs (new bg img) 0
Frames collected: 128037
Skipped frames: 22278
Rescued Frames: 2265559
Samples DAQ: 32010537
Samples written: 64018646
Samples per Channel: 32009323
FPS: 1997.2

Samples per second: 499301
Expected samples/s: 500000

Events: 8373
Events per second: 130.607
jumped back (PMT): 4132
#Frames read, frequency
# 0 frames :198723
# 1 frames :44310
# 2 frames :3284
# 3 frames :1171
# 4 frames :558
# 5 frames :1982
# 6 frames :1239
# 7 frames :963
# 8 frames :520
# 9 frames :380
# 10 frames :696
# 11 frames :335
# 12 frames :343
# 13 frames :226
# 14 frames :183
# 15 frames :158
# 16 frames :118
# 17 frames :119
# 18 frames :97
# 19 frames :86
# 20 frames :51
# 21 frames :41
# 22 frames :39
# 23 frames :36
# 24 frames :26
# 25 frames :21
# 26 frames :18
# 27 frames :26
# 28 frames :19
# 29 frames :12
# 30 frames :13
# 31 frames :9
# 32 frames :5
# 33 frames :8
# 34 frames :9
# 35 frames :3
# 36 frames :4
# 37 frames :7
# 38 frames :2
# 39 frames :3
# 40 frames :4
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 46 frames :2
# 47 frames :1
# 51 frames :1
# 53 frames :2
# 56 frames :1
# 57 frames :1
# 63 frames :1
# 71 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5302
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0