Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.59.51 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "208d14f5ba6644c3f28288d5ef8ce559",
"name": "M1_20170615_1917_dsRNA_X7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:00:16"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9878 (max 9879) in '9878'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5221
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.44384e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 32 mean_circ: 0.954155
Ran for 1050 frames Cell Events: 59 mean_circ: 0.932674
Ran for 2050 frames Cell Events: 120 mean_circ: 0.934479
Ran for 3550 frames Cell Events: 255 mean_circ: 0.936142
Ran for 4050 frames Cell Events: 285 mean_circ: 0.933172
Ran for 4550 frames Cell Events: 325 mean_circ: 0.932133
Ran for 9550 frames Cell Events: 808 mean_circ: 0.931545
Ran for 10050 frames Cell Events: 845 mean_circ: 0.931545
Ran for 12050 frames Cell Events: 973 mean_circ: 0.931077
Ran for 12550 frames Cell Events: 1004 mean_circ: 0.931197
Ran for 14550 frames Cell Events: 1166 mean_circ: 0.931504
Ran for 15050 frames Cell Events: 1208 mean_circ: 0.931147
Ran for 16550 frames Cell Events: 1315 mean_circ: 0.930959
Ran for 17050 frames Cell Events: 1350 mean_circ: 0.931122
Ran for 19050 frames Cell Events: 1525 mean_circ: 0.930858
Ran for 19550 frames Cell Events: 1565 mean_circ: 0.930915
Ran for 22050 frames Cell Events: 1757 mean_circ: 0.930305
Ran for 23050 frames Cell Events: 1825 mean_circ: 0.929929
Ran for 24050 frames Cell Events: 1902 mean_circ: 0.930163
Ran for 24550 frames Cell Events: 1930 mean_circ: 0.930285
Ran for 26550 frames Cell Events: 2084 mean_circ: 0.930419
Ran for 28050 frames Cell Events: 2145 mean_circ: 0.930471
Ran for 28550 frames Cell Events: 2182 mean_circ: 0.930493
Ran for 29550 frames Cell Events: 2257 mean_circ: 0.930872
Ran for 30550 frames Cell Events: 2320 mean_circ: 0.930905
Ran for 31550 frames Cell Events: 2389 mean_circ: 0.930591
Ran for 32050 frames Cell Events: 2411 mean_circ: 0.930574
Ran for 32550 frames Cell Events: 2451 mean_circ: 0.930683
Ran for 33050 frames Cell Events: 2482 mean_circ: 0.930554
Ran for 33550 frames Cell Events: 2515 mean_circ: 0.930554
Ran for 37050 frames Cell Events: 2753 mean_circ: 0.930383
Ran for 37550 frames Cell Events: 2784 mean_circ: 0.930446
Ran for 38050 frames Cell Events: 2824 mean_circ: 0.930476
Ran for 42550 frames Cell Events: 3185 mean_circ: 0.930071
Ran for 43050 frames Cell Events: 3229 mean_circ: 0.93013
Ran for 44050 frames Cell Events: 3302 mean_circ: 0.930175
Ran for 45050 frames Cell Events: 3386 mean_circ: 0.930107
Ran for 45550 frames Cell Events: 3430 mean_circ: 0.930386
Ran for 46050 frames Cell Events: 3460 mean_circ: 0.93039
Ran for 46550 frames Cell Events: 3500 mean_circ: 0.930544
Ran for 47050 frames Cell Events: 3537 mean_circ: 0.930399
Ran for 47550 frames Cell Events: 3563 mean_circ: 0.93027
Ran for 49550 frames Cell Events: 3714 mean_circ: 0.930343
Ran for 51050 frames Cell Events: 3846 mean_circ: 0.930249
Ran for 51550 frames Cell Events: 3876 mean_circ: 0.930318
Ran for 52050 frames Cell Events: 3914 mean_circ: 0.930332
Ran for 52550 frames Cell Events: 3952 mean_circ: 0.930285
Ran for 53550 frames Cell Events: 4022 mean_circ: 0.93016
Ran for 54050 frames Cell Events: 4063 mean_circ: 0.93013
Ran for 55550 frames Cell Events: 4189 mean_circ: 0.930003
Ran for 56550 frames Cell Events: 4254 mean_circ: 0.930159
Ran for 57550 frames Cell Events: 4308 mean_circ: 0.930164
Ran for 58050 frames Cell Events: 4341 mean_circ: 0.930134
Ran for 58550 frames Cell Events: 4371 mean_circ: 0.930109
Ran for 59050 frames Cell Events: 4410 mean_circ: 0.930119
Ran for 59550 frames Cell Events: 4451 mean_circ: 0.930245
Ran for 61550 frames Cell Events: 4606 mean_circ: 0.930084
Ran for 64050 frames Cell Events: 4824 mean_circ: 0.930132
Ran for 65550 frames Cell Events: 4970 mean_circ: 0.930105
Ran for 66550 frames Cell Events: 5035 mean_circ: 0.930054
Ran for 69050 frames Cell Events: 5183 mean_circ: 0.930177
Ran for 70050 frames Cell Events: 5245 mean_circ: 0.930249
Ran for 71050 frames Cell Events: 5321 mean_circ: 0.930272
Ran for 73550 frames Cell Events: 5512 mean_circ: 0.930141
Ran for 75050 frames Cell Events: 5600 mean_circ: 0.930099
Ran for 76050 frames Cell Events: 5677 mean_circ: 0.929983
Ran for 76550 frames Cell Events: 5716 mean_circ: 0.929883
Ran for 77050 frames Cell Events: 5749 mean_circ: 0.929932
Ran for 78050 frames Cell Events: 5812 mean_circ: 0.929933
Ran for 78550 frames Cell Events: 5850 mean_circ: 0.929917
Ran for 79050 frames Cell Events: 5887 mean_circ: 0.929924
Ran for 82550 frames Cell Events: 6125 mean_circ: 0.929707
Ran for 83550 frames Cell Events: 6175 mean_circ: 0.929674
Ran for 84550 frames Cell Events: 6237 mean_circ: 0.929639
Ran for 86550 frames Cell Events: 6401 mean_circ: 0.929696
Ran for 87050 frames Cell Events: 6421 mean_circ: 0.929736
Ran for 87550 frames Cell Events: 6447 mean_circ: 0.929762
Ran for 89050 frames Cell Events: 6560 mean_circ: 0.929659
Ran for 89550 frames Cell Events: 6590 mean_circ: 0.929564
Ran for 90050 frames Cell Events: 6618 mean_circ: 0.929583
Ran for 92550 frames Cell Events: 6773 mean_circ: 0.929497
Ran for 93050 frames Cell Events: 6797 mean_circ: 0.929554
Ran for 93550 frames Cell Events: 6836 mean_circ: 0.929609
Ran for 94550 frames Cell Events: 6898 mean_circ: 0.929597
Ran for 96050 frames Cell Events: 7014 mean_circ: 0.92965
Ran for 96550 frames Cell Events: 7055 mean_circ: 0.929669
Ran for 97050 frames Cell Events: 7089 mean_circ: 0.92971
Ran for 97550 frames Cell Events: 7116 mean_circ: 0.929731
Ran for 98550 frames Cell Events: 7186 mean_circ: 0.929752
Ran for 100050 frames Cell Events: 7285 mean_circ: 0.929625
Ran for 101550 frames Cell Events: 7377 mean_circ: 0.929693
Ran for 102050 frames Cell Events: 7410 mean_circ: 0.929669
Ran for 103050 frames Cell Events: 7476 mean_circ: 0.929639
Ran for 104050 frames Cell Events: 7535 mean_circ: 0.929647
Ran for 107550 frames Cell Events: 7831 mean_circ: 0.929512
Ran for 108050 frames Cell Events: 7857 mean_circ: 0.929535
Ran for 110550 frames Cell Events: 8084 mean_circ: 0.929606
Ran for 112550 frames Cell Events: 8215 mean_circ: 0.929534
Ran for 113550 frames Cell Events: 8275 mean_circ: 0.929561
Ran for 116050 frames Cell Events: 8516 mean_circ: 0.929481
Ran for 116550 frames Cell Events: 8548 mean_circ: 0.929617
Ran for 117050 frames Cell Events: 8580 mean_circ: 0.929618
Ran for 117550 frames Cell Events: 8612 mean_circ: 0.929563
Ran for 119050 frames Cell Events: 8732 mean_circ: 0.929527
Ran for 119550 frames Cell Events: 8764 mean_circ: 0.929497
Ran for 122550 frames Cell Events: 9034 mean_circ: 0.929433
Ran for 123550 frames Cell Events: 9085 mean_circ: 0.92943
Ran for 124050 frames Cell Events: 9108 mean_circ: 0.929434
Ran for 124550 frames Cell Events: 9137 mean_circ: 0.929447
Ran for 125050 frames Cell Events: 9179 mean_circ: 0.92946
Ran for 126050 frames Cell Events: 9258 mean_circ: 0.929439
Ran for 127050 frames Cell Events: 9357 mean_circ: 0.929448
Ran for 128050 frames Cell Events: 9456 mean_circ: 0.929401
Ran for 128550 frames Cell Events: 9482 mean_circ: 0.929387
Ran for 129050 frames Cell Events: 9515 mean_circ: 0.929383
Ran for 132550 frames Cell Events: 9802 mean_circ: 0.929293
Ran for 133050 frames Cell Events: 9844 mean_circ: 0.929357
Ran for 133550 frames Cell Events: 9879 mean_circ: 0.92936



End this measurment..



Stopping time: 1.44451e+008
****************************************
Measurement finished

Mean Circularitiy: 0.929344
Mean Area: 645.411
Mean FL-1 intensity: 3293.7
Mean FL-2 intensity: 3125.57
Mean FL-3 intensity: 0

frames processed: 132257
Total Time: 66884.2ms
Number of Runs (new bg img) 0
Frames collected: 133615
Skipped frames: 23636
Rescued Frames: 2344953
Samples DAQ: 33403658
Samples written: 66807220
Samples per Channel: 33403610
FPS: 1997.71

Samples per second: 499425
Expected samples/s: 500000

Events: 9879
Events per second: 147.703
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :213749
# 1 frames :39639
# 2 frames :2922
# 3 frames :1214
# 4 frames :558
# 5 frames :2234
# 6 frames :1204
# 7 frames :961
# 8 frames :615
# 9 frames :488
# 10 frames :682
# 11 frames :363
# 12 frames :346
# 13 frames :262
# 14 frames :200
# 15 frames :165
# 16 frames :130
# 17 frames :130
# 18 frames :94
# 19 frames :87
# 20 frames :78
# 21 frames :34
# 22 frames :55
# 23 frames :47
# 24 frames :53
# 25 frames :38
# 26 frames :39
# 27 frames :33
# 28 frames :32
# 29 frames :10
# 30 frames :15
# 31 frames :20
# 32 frames :15
# 33 frames :14
# 34 frames :14
# 35 frames :12
# 36 frames :9
# 37 frames :9
# 38 frames :5
# 39 frames :4
# 40 frames :7
# 41 frames :5
# 42 frames :6
# 43 frames :8
# 44 frames :4
# 45 frames :1
# 47 frames :1
# 48 frames :1
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 60 frames :1
# 61 frames :1
# 73 frames :2
# 74 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5221
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0