Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.04.22 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "178bb3902e4c5eafd01450f752513edc",
"name": "M1_20170615_2010_dsRNA_X8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:04:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10002 (max 10003) in
'10002'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1109
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.47579e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 32 mean_circ: 0.925473
Ran for 1050 frames Cell Events: 48 mean_circ: 0.922694
Ran for 2050 frames Cell Events: 85 mean_circ: 0.929137
Ran for 3050 frames Cell Events: 124 mean_circ: 0.931213
Ran for 3550 frames Cell Events: 138 mean_circ: 0.92739
Ran for 4550 frames Cell Events: 171 mean_circ: 0.928817
Ran for 5050 frames Cell Events: 206 mean_circ: 0.931808
Ran for 6050 frames Cell Events: 256 mean_circ: 0.932752
Ran for 6550 frames Cell Events: 279 mean_circ: 0.933723
Ran for 7050 frames Cell Events: 294 mean_circ: 0.933685
Ran for 7550 frames Cell Events: 315 mean_circ: 0.933329
Ran for 8550 frames Cell Events: 365 mean_circ: 0.933179
Ran for 9050 frames Cell Events: 388 mean_circ: 0.93354
Ran for 10050 frames Cell Events: 436 mean_circ: 0.9344
Ran for 11050 frames Cell Events: 482 mean_circ: 0.934842
Ran for 12050 frames Cell Events: 528 mean_circ: 0.935088
Ran for 12550 frames Cell Events: 552 mean_circ: 0.934955
Ran for 13550 frames Cell Events: 595 mean_circ: 0.935161
Ran for 14050 frames Cell Events: 627 mean_circ: 0.934808
Ran for 14550 frames Cell Events: 647 mean_circ: 0.934162
Ran for 15050 frames Cell Events: 663 mean_circ: 0.934585
Ran for 16050 frames Cell Events: 694 mean_circ: 0.933912
Ran for 17550 frames Cell Events: 774 mean_circ: 0.933592
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Ran for 19050 frames Cell Events: 824 mean_circ: 0.933515
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Ran for 22050 frames Cell Events: 953 mean_circ: 0.933522
Ran for 22550 frames Cell Events: 973 mean_circ: 0.933814
Ran for 23050 frames Cell Events: 990 mean_circ: 0.933625
Ran for 23550 frames Cell Events: 1011 mean_circ: 0.9338
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Ran for 35050 frames Cell Events: 1496 mean_circ: 0.933078
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Ran for 38050 frames Cell Events: 1638 mean_circ: 0.932405
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Ran for 44050 frames Cell Events: 1894 mean_circ: 0.931961
Ran for 45050 frames Cell Events: 1935 mean_circ: 0.931813
Ran for 45550 frames Cell Events: 1950 mean_circ: 0.93186
Ran for 46050 frames Cell Events: 1967 mean_circ: 0.931537
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Ran for 54550 frames Cell Events: 2309 mean_circ: 0.932033
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Ran for 56550 frames Cell Events: 2397 mean_circ: 0.932409
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Ran for 63050 frames Cell Events: 2650 mean_circ: 0.932776
Ran for 63550 frames Cell Events: 2672 mean_circ: 0.932716
Ran for 64550 frames Cell Events: 2709 mean_circ: 0.932544
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Ran for 65550 frames Cell Events: 2761 mean_circ: 0.932668
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Ran for 66550 frames Cell Events: 2804 mean_circ: 0.932765
Ran for 67050 frames Cell Events: 2827 mean_circ: 0.93281
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Ran for 70550 frames Cell Events: 2966 mean_circ: 0.932604
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Ran for 71550 frames Cell Events: 2992 mean_circ: 0.932431
Ran for 72050 frames Cell Events: 2999 mean_circ: 0.932499
Ran for 73050 frames Cell Events: 3053 mean_circ: 0.932594
Ran for 73550 frames Cell Events: 3071 mean_circ: 0.932586
Ran for 74050 frames Cell Events: 3085 mean_circ: 0.93261
Ran for 75050 frames Cell Events: 3117 mean_circ: 0.932567
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Ran for 76050 frames Cell Events: 3160 mean_circ: 0.932679
Ran for 77550 frames Cell Events: 3243 mean_circ: 0.932438
Ran for 78550 frames Cell Events: 3299 mean_circ: 0.932325
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Ran for 80050 frames Cell Events: 3360 mean_circ: 0.932319
Ran for 80550 frames Cell Events: 3381 mean_circ: 0.932313
Ran for 81050 frames Cell Events: 3397 mean_circ: 0.932349
Ran for 81550 frames Cell Events: 3412 mean_circ: 0.93241
Ran for 82550 frames Cell Events: 3455 mean_circ: 0.932274
Ran for 83050 frames Cell Events: 3471 mean_circ: 0.932287
Ran for 83550 frames Cell Events: 3490 mean_circ: 0.93221
Ran for 84050 frames Cell Events: 3500 mean_circ: 0.932258
Ran for 84550 frames Cell Events: 3513 mean_circ: 0.932174
Ran for 85050 frames Cell Events: 3528 mean_circ: 0.932192
Ran for 86550 frames Cell Events: 3605 mean_circ: 0.932372
Ran for 87050 frames Cell Events: 3625 mean_circ: 0.93244
Ran for 87550 frames Cell Events: 3638 mean_circ: 0.932465
Ran for 89050 frames Cell Events: 3699 mean_circ: 0.932569
Ran for 90050 frames Cell Events: 3733 mean_circ: 0.932483
Ran for 90550 frames Cell Events: 3763 mean_circ: 0.932521
Ran for 91050 frames Cell Events: 3790 mean_circ: 0.932533
Ran for 91550 frames Cell Events: 3807 mean_circ: 0.932345
Ran for 92050 frames Cell Events: 3829 mean_circ: 0.932265
Ran for 92550 frames Cell Events: 3843 mean_circ: 0.932196
Ran for 93550 frames Cell Events: 3882 mean_circ: 0.932104
Ran for 94550 frames Cell Events: 3915 mean_circ: 0.932133
Ran for 95050 frames Cell Events: 3931 mean_circ: 0.932148
Ran for 96050 frames Cell Events: 3966 mean_circ: 0.932053
Ran for 96550 frames Cell Events: 3981 mean_circ: 0.932064
Ran for 97050 frames Cell Events: 3992 mean_circ: 0.932048
Ran for 97550 frames Cell Events: 4012 mean_circ: 0.932022
Ran for 98050 frames Cell Events: 4032 mean_circ: 0.932059
Ran for 99050 frames Cell Events: 4067 mean_circ: 0.931947
Ran for 99550 frames Cell Events: 4084 mean_circ: 0.931991
Ran for 100550 frames Cell Events: 4138 mean_circ: 0.932057
Ran for 101550 frames Cell Events: 4178 mean_circ: 0.932081
Ran for 102050 frames Cell Events: 4194 mean_circ: 0.932058
Ran for 103050 frames Cell Events: 4248 mean_circ: 0.932099
Ran for 104550 frames Cell Events: 4327 mean_circ: 0.931994
Ran for 105050 frames Cell Events: 4345 mean_circ: 0.931965
Ran for 106050 frames Cell Events: 4389 mean_circ: 0.931912
Ran for 106550 frames Cell Events: 4404 mean_circ: 0.931855
Ran for 107550 frames Cell Events: 4455 mean_circ: 0.931811
Ran for 108050 frames Cell Events: 4475 mean_circ: 0.931819
Ran for 109550 frames Cell Events: 4557 mean_circ: 0.931736
Ran for 110050 frames Cell Events: 4577 mean_circ: 0.931718
Ran for 111050 frames Cell Events: 4615 mean_circ: 0.931697
Ran for 111550 frames Cell Events: 4628 mean_circ: 0.931796
Ran for 112050 frames Cell Events: 4649 mean_circ: 0.931829
Ran for 112550 frames Cell Events: 4667 mean_circ: 0.931747
Ran for 113550 frames Cell Events: 4709 mean_circ: 0.931742
Ran for 114050 frames Cell Events: 4729 mean_circ: 0.931802
Ran for 116550 frames Cell Events: 4834 mean_circ: 0.931778
Ran for 117050 frames Cell Events: 4863 mean_circ: 0.931836
Ran for 117550 frames Cell Events: 4878 mean_circ: 0.931817
Ran for 118050 frames Cell Events: 4906 mean_circ: 0.931775
Ran for 118550 frames Cell Events: 4926 mean_circ: 0.931775
Ran for 119550 frames Cell Events: 4962 mean_circ: 0.931668
Ran for 120050 frames Cell Events: 4978 mean_circ: 0.931678
Ran for 121550 frames Cell Events: 5031 mean_circ: 0.931731
Ran for 122550 frames Cell Events: 5079 mean_circ: 0.931659
Ran for 123050 frames Cell Events: 5105 mean_circ: 0.931584
Ran for 123550 frames Cell Events: 5133 mean_circ: 0.931515
Ran for 124050 frames Cell Events: 5151 mean_circ: 0.93158
Ran for 124550 frames Cell Events: 5177 mean_circ: 0.931605
Ran for 125050 frames Cell Events: 5197 mean_circ: 0.931608
Ran for 125550 frames Cell Events: 5222 mean_circ: 0.931597
Ran for 127050 frames Cell Events: 5308 mean_circ: 0.931653
Ran for 129050 frames Cell Events: 5419 mean_circ: 0.931743
Ran for 129550 frames Cell Events: 5444 mean_circ: 0.931711
Ran for 130550 frames Cell Events: 5488 mean_circ: 0.931586
Ran for 131050 frames Cell Events: 5508 mean_circ: 0.931623
Ran for 131550 frames Cell Events: 5525 mean_circ: 0.931597
Ran for 132050 frames Cell Events: 5543 mean_circ: 0.93154
Ran for 133050 frames Cell Events: 5584 mean_circ: 0.931517
Ran for 133550 frames Cell Events: 5603 mean_circ: 0.93149
Ran for 134550 frames Cell Events: 5642 mean_circ: 0.931516
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Ran for 140550 frames Cell Events: 5932 mean_circ: 0.931438
Ran for 141550 frames Cell Events: 5997 mean_circ: 0.931428
Ran for 143050 frames Cell Events: 6076 mean_circ: 0.931358
Ran for 143550 frames Cell Events: 6086 mean_circ: 0.931369
Ran for 144050 frames Cell Events: 6111 mean_circ: 0.931374
Ran for 144550 frames Cell Events: 6141 mean_circ: 0.931259
Ran for 145550 frames Cell Events: 6172 mean_circ: 0.931266
Ran for 147550 frames Cell Events: 6247 mean_circ: 0.931233
Ran for 148550 frames Cell Events: 6288 mean_circ: 0.931196
Ran for 149050 frames Cell Events: 6307 mean_circ: 0.931182
Ran for 149550 frames Cell Events: 6329 mean_circ: 0.931188
Ran for 150550 frames Cell Events: 6380 mean_circ: 0.931209
Ran for 151050 frames Cell Events: 6397 mean_circ: 0.931251
Ran for 151550 frames Cell Events: 6410 mean_circ: 0.931253
Ran for 152550 frames Cell Events: 6449 mean_circ: 0.931274
Ran for 153050 frames Cell Events: 6472 mean_circ: 0.931276
Ran for 153550 frames Cell Events: 6488 mean_circ: 0.931285
Ran for 154050 frames Cell Events: 6509 mean_circ: 0.931232
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Ran for 156050 frames Cell Events: 6603 mean_circ: 0.931086
Ran for 157550 frames Cell Events: 6654 mean_circ: 0.931118
Ran for 158050 frames Cell Events: 6670 mean_circ: 0.931069
Ran for 159050 frames Cell Events: 6717 mean_circ: 0.931065
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Ran for 162050 frames Cell Events: 6825 mean_circ: 0.931081
Ran for 163050 frames Cell Events: 6885 mean_circ: 0.931059
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Ran for 174550 frames Cell Events: 7359 mean_circ: 0.93097
Ran for 176050 frames Cell Events: 7426 mean_circ: 0.930833
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Ran for 181550 frames Cell Events: 7656 mean_circ: 0.930788
Ran for 182050 frames Cell Events: 7684 mean_circ: 0.930756
Ran for 183550 frames Cell Events: 7734 mean_circ: 0.930688
Ran for 184550 frames Cell Events: 7765 mean_circ: 0.930712
Ran for 185050 frames Cell Events: 7780 mean_circ: 0.930653
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Ran for 189050 frames Cell Events: 7950 mean_circ: 0.930645
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Ran for 190050 frames Cell Events: 7994 mean_circ: 0.930572
Ran for 191050 frames Cell Events: 8033 mean_circ: 0.930551
Ran for 191550 frames Cell Events: 8062 mean_circ: 0.930519
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Ran for 196050 frames Cell Events: 8276 mean_circ: 0.930211
Ran for 196550 frames Cell Events: 8292 mean_circ: 0.93023
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Ran for 198050 frames Cell Events: 8367 mean_circ: 0.930165
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Ran for 200550 frames Cell Events: 8467 mean_circ: 0.930051
Ran for 201050 frames Cell Events: 8488 mean_circ: 0.929991
Ran for 201550 frames Cell Events: 8503 mean_circ: 0.929968
Ran for 202050 frames Cell Events: 8523 mean_circ: 0.929997
Ran for 203050 frames Cell Events: 8563 mean_circ: 0.930032
Ran for 203550 frames Cell Events: 8580 mean_circ: 0.930022
Ran for 204050 frames Cell Events: 8597 mean_circ: 0.930023
Ran for 204550 frames Cell Events: 8625 mean_circ: 0.93002
Ran for 205050 frames Cell Events: 8639 mean_circ: 0.93001
Ran for 205550 frames Cell Events: 8656 mean_circ: 0.930007
Ran for 206550 frames Cell Events: 8699 mean_circ: 0.930012
Ran for 207550 frames Cell Events: 8742 mean_circ: 0.930039
Ran for 208050 frames Cell Events: 8767 mean_circ: 0.929967
Ran for 209550 frames Cell Events: 8841 mean_circ: 0.929951
Ran for 210050 frames Cell Events: 8858 mean_circ: 0.929949
Ran for 210550 frames Cell Events: 8874 mean_circ: 0.929967
Ran for 211050 frames Cell Events: 8889 mean_circ: 0.929971
Ran for 212050 frames Cell Events: 8946 mean_circ: 0.929967
Ran for 212550 frames Cell Events: 8971 mean_circ: 0.929967
Ran for 213550 frames Cell Events: 9011 mean_circ: 0.929902
Ran for 214050 frames Cell Events: 9032 mean_circ: 0.9299
Ran for 214550 frames Cell Events: 9063 mean_circ: 0.9299
Ran for 215050 frames Cell Events: 9083 mean_circ: 0.929941
Ran for 216550 frames Cell Events: 9146 mean_circ: 0.929987
Ran for 217050 frames Cell Events: 9161 mean_circ: 0.929979
Ran for 217550 frames Cell Events: 9176 mean_circ: 0.929982
Ran for 218050 frames Cell Events: 9198 mean_circ: 0.930015
Ran for 218550 frames Cell Events: 9222 mean_circ: 0.930036
Ran for 219050 frames Cell Events: 9240 mean_circ: 0.930007
Ran for 219550 frames Cell Events: 9270 mean_circ: 0.92996
Ran for 220550 frames Cell Events: 9318 mean_circ: 0.92988
Ran for 221050 frames Cell Events: 9337 mean_circ: 0.929892
Ran for 221550 frames Cell Events: 9350 mean_circ: 0.929879
Ran for 222550 frames Cell Events: 9392 mean_circ: 0.92989
Ran for 223050 frames Cell Events: 9418 mean_circ: 0.929869
Ran for 223550 frames Cell Events: 9440 mean_circ: 0.929834
Ran for 225050 frames Cell Events: 9509 mean_circ: 0.929794
Ran for 225550 frames Cell Events: 9526 mean_circ: 0.929797
Ran for 226050 frames Cell Events: 9549 mean_circ: 0.929822
Ran for 226550 frames Cell Events: 9570 mean_circ: 0.929795
Ran for 227050 frames Cell Events: 9589 mean_circ: 0.92976
Ran for 227550 frames Cell Events: 9611 mean_circ: 0.929783
Ran for 228550 frames Cell Events: 9643 mean_circ: 0.929718
Ran for 229050 frames Cell Events: 9669 mean_circ: 0.92969
Ran for 231550 frames Cell Events: 9799 mean_circ: 0.929617
Ran for 232050 frames Cell Events: 9819 mean_circ: 0.929583
Ran for 232550 frames Cell Events: 9844 mean_circ: 0.92959
Ran for 233050 frames Cell Events: 9870 mean_circ: 0.929568
Ran for 233550 frames Cell Events: 9895 mean_circ: 0.929602
Ran for 234050 frames Cell Events: 9914 mean_circ: 0.929589
Ran for 234550 frames Cell Events: 9953 mean_circ: 0.929582
Ran for 235050 frames Cell Events: 9972 mean_circ: 0.929583
Ran for 235550 frames Cell Events: 9996 mean_circ: 0.929558



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.47697e+008
****************************************
Measurement finished

Mean Circularitiy: 0.929551
Mean Area: 698.858
Mean FL-1 intensity: 3210.34
Mean FL-2 intensity: 3658.68
Mean FL-3 intensity: 0

frames processed: 235167
Total Time: 117951ms
Number of Runs (new bg img) 0
Frames collected: 235704
Skipped frames: 30294
Rescued Frames: 3083930
Samples DAQ: 58926319
Samples written: 117851972
Samples per Channel: 58925986
FPS: 1998.32

Samples per second: 499580
Expected samples/s: 500000

Events: 10003
Events per second: 84.8064
jumped back (PMT): 6951
#Frames read, frequency
# 0 frames :342466
# 1 frames :107025
# 2 frames :6991
# 3 frames :2220
# 4 frames :1191
# 5 frames :1900
# 6 frames :2881
# 7 frames :1330
# 8 frames :785
# 9 frames :641
# 10 frames :862
# 11 frames :824
# 12 frames :523
# 13 frames :329
# 14 frames :249
# 15 frames :181
# 16 frames :178
# 17 frames :115
# 18 frames :124
# 19 frames :80
# 20 frames :70
# 21 frames :55
# 22 frames :29
# 23 frames :31
# 24 frames :34
# 25 frames :21
# 26 frames :36
# 27 frames :27
# 28 frames :22
# 29 frames :25
# 30 frames :21
# 31 frames :13
# 32 frames :10
# 33 frames :8
# 34 frames :10
# 35 frames :6
# 36 frames :3
# 37 frames :5
# 38 frames :3
# 39 frames :2
# 40 frames :3
# 41 frames :4
# 42 frames :3
# 44 frames :1
# 46 frames :1
# 47 frames :2
# 49 frames :1
# 50 frames :1
# 51 frames :1
# 54 frames :1
# 56 frames :1
# 58 frames :2
# 61 frames :2
# 62 frames :1
# 76 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1109
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0